################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI Hin1I HindIII HpaI KasI KpnI MaeI MfeI MflI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SbfI ScaI SfcI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 652 GTCGAC GT^[AC][GT]AC Alw44I 301 GTGCAC G^TGCAC AlwI 711 GATCC GGATC(4/5) ApaLI 301 GTGCAC G^TGCAC AspHI 301 GTGCAC G[AT]GC[AT]^C AviII 407 TGCGCA TGC^GCA BbsI 577 GTCTTC GAAGAC(2/6) BpuAI 577 GTCTTC GAAGAC(2/6) BsaBI 711 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 301 GTGCAC G[AT]GC[AT]^C BsmFI 1058 GTCCC GGGAC(10/14) BspMI 587 ACCTGC ACCTGC(4/8) BsrBI 793 CCGCTC CCGCTC(-3/-3) BssSI 109 CTCGTG CACGAG(-5/-1) BstAPI 911 GCACGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DdeI 481 CTGAG C^T[ACGT]AG DpnII 711 GATC ^GATC EcoT14I 557 CCATGG C^C[AT][AT]GG EcoT22I 1083 ATGCAT ATGCA^T FspI 407 TGCGCA TGC^GCA HgaI 857 GCGTC GACGC(5/10) HinfI 1012 GAGTC G^A[ACGT]TC KspI 665 CCGCGG CCGC^GG MaeII 5 ACGT A^CGT MaeIII 700 GTGAC ^GT[ACGT]AC MamI 711 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 711 GATC ^GATC MseI 194 TTAA T^TAA MslI 129 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 557 CCATGG C^CATGG NdeII 711 GATC ^GATC NsiI 1083 ATGCAT ATGCA^T PaeR7I 1090 CTCGAG C^TCGAG PleI 1012 GAGTC GAGTC(4/5) PvuII 502 CAGCTG CAG^CTG SacII 665 CCGCGG CCGC^GG SalI 652 GTCGAC G^TCGAC Sau3AI 711 GATC ^GATC SexAI 833 ACCTGGT A^CC[AT]GGT SfiI 925 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1090 CTCGAG C^T[CT][GA]AG StyI 557 CCATGG C^C[AT][AT]GG Tru9I 194 TTAA T^TAA Tsp45I 700 GTGAC ^GT[GC]AC TspRI 990 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 251 CCATCTACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1090 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AscI 644 GGCGCGCC GG^CGCGCC 683 GGCGCGCC BalI 374 TGGCCA TGG^CCA 506 TGGCCA BglI 38 GCCAGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 926 GCCTGCTGGGC BsaI 837 GGTCTC GGTCTC(1/5) 882 GGTCTC BsgI 391 CTGCAC GTGCAG(16/14) 567 CTGCAC BsmBI 6 CGTCTC CGTCTC(1/5) 354 CGTCTC BsrI 495 CCAGT ACTGG(1/-1) 786 CCAGT HaeII 538 AGCGCC [GA]GCGC^[CT] 1069 GGCGCT HincII 652 GTCGAC GT[CT]^[GA]AC 859 GTCAAC HindII 652 GTCGAC GT[CT]^[GA]AC 859 GTCAAC HphI 699 GGTGA GGTGA(8/7) 320 TCACC MluNI 374 TGGCCA TGG^CCA 506 TGGCCA MscI 374 TGGCCA TGG^CCA 506 TGGCCA ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AluI ApaI AvaI BanI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsoBI Bsp1286I BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI Ksp632I MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV SapI Sau96I ScrFI SfaNI TaqI TseI TthHB8I