(Circular) (Six-base) MAPSORT of: pFN21AE5275.seq Check: 6278 from: 1 to: 5356 pFN21AE5275 5356 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2027 3166 278 Size: 53 83 186 936 491 1139 2468 Fragments arranged by size: 2468 1139 936 491 186 83 53 AccI GT'mk_AC Cuts at: 1937 2699 1937 Size: 762 4594 Acc65I G'GTAC_C Cuts at: 2677 2677 Size: 5356 AclI AA'CG_TT Cuts at: 1563 3483 3856 1563 Size: 1920 373 3063 Fragments arranged by size: 3063 1920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 2444 2563 3419 4803 5071 1632 Size: 581 231 119 856 1384 268 1917 Fragments arranged by size: 1917 1384 856 581 268 231 119 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5159 AflIII A'CryG_T Cuts at: 1203 4255 5232 1203 Size: 3052 977 1327 Fragments arranged by size: 3052 1327 977 AgeI A'CCGG_T Cuts at: 4187 4187 Size: 5356 AhdI GACnn_n'nnGTC Cuts at: 4085 4085 Size: 5356 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2336 1184 Size: 1152 4204 AlwNI CAG_nnn'CTG Cuts at: 3099 4671 3099 Size: 1572 3784 ApaLI G'TGCA_C Cuts at: 3413 4569 3413 Size: 1156 4200 ApoI r'AATT_y Cuts at: 1493 2665 2891 3043 5152 1493 Size: 1172 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 226 152 AseI AT'TA_AT Cuts at: 160 1032 3910 160 Size: 872 2878 1606 Fragments arranged by size: 2878 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 AvaI C'yCGr_G Cuts at: 1870 1943 2681 1870 Size: 73 738 4545 Fragments arranged by size: 4545 738 73 BamHI G'GATC_C Cuts at: 2686 3053 2686 Size: 367 4989 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2393 2677 4132 618 Size: 325 217 627 606 284 1455 1842 Fragments arranged by size: 1842 1455 627 606 325 284 217 BanII G_rGCy'C Cuts at: 1391 2349 2675 1391 Size: 958 326 4072 Fragments arranged by size: 4072 958 326 BbeI G_GCGC'C Cuts at: 2397 2397 Size: 5356 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2362 928 Size: 971 463 3922 Fragments arranged by size: 3922 971 463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3547 3581 1903 Size: 34 1610 34 3678 Fragments arranged by size: 3678 1610 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3248 4458 3248 Size: 1210 4146 BclI T'GATC_A Cuts at: 1548 1548 Size: 5356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3967 136 Size: 107 122 71 631 605 2295 1525 Fragments arranged by size: 2295 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2493 5351 2493 Size: 2858 2498 BlpI GC'TnA_GC Cuts at: 2773 2773 Size: 5356 Bme1580I G_kGCm'C Cuts at: 1790 3417 4573 1790 Size: 1627 1156 2573 Fragments arranged by size: 2573 1627 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2496 3087 4045 Size: 416 53 83 399 24 1079 591 958 Cuts at: 4045 442 Size: 1753 Fragments arranged by size: 1753 1079 958 591 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 4016 1239 Size: 1124 1653 2579 Fragments arranged by size: 2579 1653 1124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2537 3481 4346 4644 2537 Size: 944 865 298 3249 Fragments arranged by size: 3249 944 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4019 882 Size: 617 2520 2219 Fragments arranged by size: 2520 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3147 493 Size: 711 1943 2702 Fragments arranged by size: 2702 1943 711 BsaBI GATnn'nnATC Cuts at: 5350 5350 Size: 5356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 2394 3163 3545 Size: 53 83 186 936 491 370 769 382 Cuts at: 3545 4180 275 Size: 635 1451 Fragments arranged by size: 1451 936 769 635 491 382 370 186 83 53 BsaWI w'CCGG_w Cuts at: 3789 4187 4461 4608 5019 3789 Size: 398 274 147 411 4126 Fragments arranged by size: 4126 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 1178 Size: 30 165 30 786 30 4315 Fragments arranged by size: 4315 786 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 1186 Size: 1162 4194 BseYI C'CCAG_C Cuts at: 4559 5055 4559 Size: 496 4860 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2372 2642 1163 Size: 573 636 270 3877 Fragments arranged by size: 3877 636 573 270 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2806 3567 4595 1540 Size: 275 186 805 761 1028 2301 Fragments arranged by size: 2301 1028 805 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3417 3502 4573 3417 Size: 85 1071 4200 Fragments arranged by size: 4200 1071 85 BsmI GAATG_Cn' Cuts at: 2867 2960 2867 Size: 93 5263 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2349 2675 3417 3502 4573 1391 Size: 399 559 326 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 559 399 326 85 BspEI T'CCGG_A Cuts at: 5019 5019 Size: 5356 BspHI T'CATG_A Cuts at: 3245 4975 3245 Size: 1730 3626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2711 844 Size: 1867 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2631 3243 1394 Size: 429 808 612 3507 Fragments arranged by size: 3507 808 612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3851 4025 65 Size: 1183 2603 174 1396 Fragments arranged by size: 2603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4000 4187 1700 Size: 110 2190 187 2869 Fragments arranged by size: 2869 2190 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5356 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5356 BssSI C'ACGA_G Cuts at: 3416 4428 3416 Size: 1012 4344 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5356 BstBI TT'CG_AA Cuts at: 4160 4160 Size: 5356 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5356 BstXI CCAn_nnnn'nTGG Cuts at: 2383 2383 Size: 5356 BstYI r'GATC_y Cuts at: 1972 2493 2686 3053 3438 3455 4907 4993 5005 Size: 521 193 367 385 17 1452 86 12 Cuts at: 5005 5351 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 521 385 367 346 193 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2360 2360 Size: 5356 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3865 Fragments arranged by size: 3865 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4617 Fragments arranged by size: 4617 683 56 BtsI GCAGTG_nn' Cuts at: 2083 2546 2630 2872 3666 3686 2083 Size: 463 84 242 794 20 3753 Fragments arranged by size: 3753 794 463 242 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5321 DraI TTT'AAA Cuts at: 2660 2660 Size: 5356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2408 4363 809 Size: 507 1092 1955 1802 Fragments arranged by size: 1955 1802 1092 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2803 3692 5321 Size: 54 1425 50 275 18 973 889 1629 Cuts at: 5321 8 Size: 43 Fragments arranged by size: 1629 1425 973 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2803 1537 Size: 275 991 4090 Fragments arranged by size: 4090 991 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3286 1353 Size: 1933 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3943 4317 4463 387 Size: 1064 138 2354 374 146 1280 Fragments arranged by size: 2354 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2363 2444 2563 3419 4016 4803 Size: 393 581 150 81 119 856 597 787 Cuts at: 4803 5071 1239 Size: 268 1524 Fragments arranged by size: 1524 856 787 597 581 393 268 150 119 81 EcoNI CCTnn'n_nnAGG Cuts at: 2101 2101 Size: 5356 EcoO109I rG'GnC_Cy Cuts at: 2428 2428 Size: 5356 EcoRI G'AATT_C Cuts at: 2665 3043 2665 Size: 378 4978 FspI TGC'GCA Cuts at: 2615 3862 2615 Size: 1247 4109 HaeII r_GCGC'y Cuts at: 1678 2397 4503 1678 Size: 719 2106 2531 Fragments arranged by size: 2531 2106 719 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2485 2513 2517 2545 3997 Size: 32 135 32 913 28 4 28 1452 Cuts at: 3997 4029 4071 4103 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1452 913 135 42 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2700 2946 669 Size: 988 281 762 246 3079 Fragments arranged by size: 3079 988 762 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5356 HpaI GTT'AAC Cuts at: 2946 2946 Size: 5356 KasI G'GCGC_C Cuts at: 2393 2393 Size: 5356 KpnI G_GTAC'C Cuts at: 2681 2681 Size: 5356 MfeI C'AATT_G Cuts at: 2955 2955 Size: 5356 MluI A'CGCG_T Cuts at: 5232 5232 Size: 5356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2282 2292 4470 4654 1305 Size: 977 10 2178 184 2007 Fragments arranged by size: 2178 2007 977 184 10 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3534 Fragments arranged by size: 3534 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2538 3314 3673 3832 518 Size: 648 1372 776 359 159 2042 Fragments arranged by size: 2042 1372 776 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2149 2646 2772 3449 4597 4842 1606 Size: 543 497 126 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 543 497 245 126 NarI GG'CG_CC Cuts at: 2394 2394 Size: 5356 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3865 Fragments arranged by size: 3865 939 552 NdeI CA'TA_TG Cuts at: 387 2155 387 Size: 1768 3588 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5356 NotI GC'GGCC_GC Cuts at: 2803 2803 Size: 5356 NspI r_CATG'y Cuts at: 1132 1229 2714 4259 1132 Size: 97 1485 1545 2229 Fragments arranged by size: 2229 1545 1485 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5356 PciI A'CATG_T Cuts at: 4255 4255 Size: 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5356 PfoI T'CCnGG_A Cuts at: 2018 2018 Size: 5356 PmeI GTTT'AAAC Cuts at: 2660 2660 Size: 5356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3425 3457 4938 4970 3425 Size: 32 1481 32 3811 Fragments arranged by size: 3811 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2428 2428 Size: 5356 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5356 PsiI TTA'TAA Cuts at: 2926 2926 Size: 5356 PstI C_TGCA'G Cuts at: 830 2014 2220 2708 830 Size: 1184 206 488 3478 Fragments arranged by size: 3478 1184 488 206 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5356 PvuII CAG'CTG Cuts at: 2149 2646 2149 Size: 497 4859 SalI G'TCGA_C Cuts at: 1936 2698 1936 Size: 762 4594 SbfI CC_TGCA'GG Cuts at: 2708 2708 Size: 5356 ScaI AGT'ACT Cuts at: 3604 5316 3604 Size: 1712 3644 SfcI C'TryA_G Cuts at: 826 1045 2010 2216 2509 2704 3839 4520 4711 Size: 219 965 206 293 195 1135 681 191 Cuts at: 4711 826 Size: 1471 Fragments arranged by size: 1471 1135 965 681 293 219 206 195 191 SfoI GGC'GCC Cuts at: 2395 2395 Size: 5356 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5356 SmaI CCC'GGG Cuts at: 1872 2683 1872 Size: 811 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 3460 4361 4623 820 Size: 197 926 609 908 901 262 1553 Fragments arranged by size: 1553 926 908 901 609 262 197 SnaBI TAC'GTA Cuts at: 493 3147 493 Size: 2654 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5356 SphI G_CATG'C Cuts at: 1132 2714 1132 Size: 1582 3774 SspI AAT'ATT Cuts at: 5 52 3280 5 Size: 47 3228 2081 Fragments arranged by size: 3228 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5356 StyI C'CwwG_G Cuts at: 513 1065 2004 2795 513 Size: 552 939 791 3074 Fragments arranged by size: 3074 939 791 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3383 3400 3553 3738 4183 325 Size: 1255 1803 17 153 185 445 1498 Fragments arranged by size: 1803 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3602 5314 96 Size: 274 80 33 51 1443 1625 1712 138 Fragments arranged by size: 1712 1625 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5316 5348 5316 Size: 32 5324 Tth111I GACn'n_nGTC Cuts at: 1316 2597 1316 Size: 1281 4075 XbaI T'CTAG_A Cuts at: 2692 2692 Size: 5356 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5356 XmaI C'CCGG_G Cuts at: 1870 2681 1870 Size: 811 4545 XmnI GAAnn'nnTTC Cuts at: 3485 3485 Size: 5356 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2025 3164 276 Size: 53 83 186 936 491 1139 2468 Fragments arranged by size: 2468 1139 936 491 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI