################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NarI NdeI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AlwI 296 GATCC GGATC(4/5) AlwNI 502 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 171 CCTCAGG CC^T[ACGT]AGG ApaI 57 GGGCCC GGGCC^C AspHI 316 GTGCTC G[AT]GC[AT]^C BanII 57 GGGCCC G[GA]GC[CT]^C BbsI 646 GTCTTC GAAGAC(2/6) BciVI 231 GTATCC GTATCC(6/5) BfaI 25 CTAG C^TAG BpuAI 646 GTCTTC GAAGAC(2/6) BsgI 352 CTGCAC GTGCAG(16/14) BsiHKAI 316 GTGCTC G[AT]GC[AT]^C BsmFI 199 GGGAC GGGAC(10/14) BspMI 640 GCAGGT ACCTGC(4/8) BsrI 61 CCAGT ACTGG(1/-1) BstYI 295 AGATCC [GA]^GATC[CT] Bsu36I 171 CCTCAGG CC^T[ACGT]AGG CfoI 638 GCGC GCG^C CpoI 611 CGGACCG CG^G[AT]CCG DpnII 296 GATC ^GATC Eco81I 171 CCTCAGG CC^T[ACGT]AGG HhaI 638 GCGC GCG^C HinP1I 638 GCGC G^CGC MaeI 25 CTAG C^TAG MaeII 518 ACGT A^CGT MboI 296 GATC ^GATC MflI 295 AGATCC [GA]^GATC[CT] MspA1I 627 CAGCTG C[AC]G^C[GT]G NdeII 296 GATC ^GATC NheI 24 GCTAGC G^CTAGC PaeR7I 548 CTCGAG C^TCGAG PpuMI 503 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 627 CAGCTG CAG^CTG RsrII 611 CGGACCG CG^G[AT]CCG Sau3AI 296 GATC ^GATC SexAI 356 ACCTGGT A^CC[AT]GGT SmaI 689 CCCGGG CCC^GGG SmlI 548 CTCGAG C^T[CT][GA]AG StuI 438 AGGCCT AGG^CCT TaqI 549 TCGA T^CGA TfiI 467 GAATC G^A[AT]TC Tsp509I 684 AATT ^AATT TspRI 314 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 549 TCGA T^CGA XcmI 530 CCATGGGTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 548 CTCGAG C^TCGAG XhoII 295 AGATCC [GA]^GATC[CT] XmaCI 689 CCCGGG C^CCGGG XmaI 689 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaI 548 CTCGAG C^[CT]CG[GA]G 689 CCCGGG AvaII 504 GGTCC G^G[AT]CC 612 GGACC BmyI 57 GGGCCC G[AGT]GC[ACT]^C 316 GTGCTC BpmI 41 CTCCAG CTGGAG(16/14) 479 CTGGAG BsoBI 548 CTCGAG C^[CT]CG[GA]G 689 CCCGGG Bsp1286I 57 GGGCCC G[AGT]GC[ACT]^C 316 GTGCTC BsrFI 72 GCCGGC [GA]^CCGG[CT] 188 GCCGGC BstF5I 384 GGATG GGATG(2/0) 474 GGATG BstXI 450 CCAAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 471 CCAGGATGCTGG Cfr10I 72 GCCGGC [GA]^CCGG[CT] 188 GCCGGC FokI 384 GGATG GGATG(9/13) 474 GGATG MaeIII 397 GTGAC ^GT[ACGT]AC 520 GTGAC MboII 333 GAAGA GAAGA(8/7) 647 TCTTC MseI 368 TTAA T^TAA 707 TTAA NaeI 72 GCCGGC GCC^GGC 188 GCCGGC NcoI 134 CCATGG C^CATGG 530 CCATGG NgoMIV 72 GCCGGC G^CCGGC 188 GCCGGC PstI 163 CTGCAG CTGCA^G 193 CTGCAG SfaNI 265 GATGC GCATC(5/9) 475 GATGC Tru9I 368 TTAA T^TAA 707 TTAA Tsp45I 397 GTGAC ^GT[GC]AC 520 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AluI BbvI BcnI BsaJI BsiYI BslI BsmAI BssKI BstNI Cac8I Cfr13I DdeI DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MnlI MspI MvaI MwoI NciI NlaIII NlaIV PleI Sau96I ScrFI SfcI StyI TseI