Restriction map for
pFN21AE1963
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI AlwI AlwNI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
DraIII Eam1105I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
KpnI MamI MfeI MflI MroI
MseI MunI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XhoII
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 901 GACGTC GACGT^C
AflIII 1028 ACGCGT A^C[GA][CT]GT
Alw44I 1008 GTGCAC G^TGCAC
AocI 1321 CCTGAGG CC^T[ACGT]AGG
Aor51HI 857 AGCGCT AGC^GCT
ApaLI 1008 GTGCAC G^TGCAC
AviII 545 TGCGCA TGC^GCA
BbeI 91 GGCGCC GGCGC^C
BbrPI 1084 CACGTG CAC^GTG
BbsI 877 GAAGAC GAAGAC(2/6)
BfaI 418 CTAG C^TAG
BpuAI 877 GAAGAC GAAGAC(2/6)
BsaAI 1084 CACGTG [CT]AC^GT[GA]
BsaI 421 GAGACC GGTCTC(1/5)
BspMI 329 ACCTGC ACCTGC(4/8)
BsrBI 223 GAGCGG CCGCTC(-3/-3)
BsrFI 952 GCCGGC [GA]^CCGG[CT]
BssSI 1069 CACGAG CACGAG(-5/-1)
BstEII 618 GGTCACC G^GT[ACGT]ACC
BstPI 618 GGTCACC G^GT[ACGT]ACC
Bsu36I 1321 CCTGAGG CC^T[ACGT]AGG
Cfr10I 952 GCCGGC [GA]^CCGG[CT]
DpnII 705 GATC ^GATC
DrdI 1356 GACGTACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 334 CTCTTC CTCTTC(1/4)
Eco47III 857 AGCGCT AGC^GCT
Eco81I 1321 CCTGAGG CC^T[ACGT]AGG
EcoO65I 618 GGTCACC G^GT[ACGT]ACC
FseI 951 GGCCGGCC GGCCGG^CC
FspI 545 TGCGCA TGC^GCA
KasI 91 GGCGCC G^GCGCC
Ksp632I 334 CTCTTC CTCTTC(1/4)
MaeI 418 CTAG C^TAG
MboI 705 GATC ^GATC
MluI 1028 ACGCGT A^CGCGT
MslI 1476 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 952 GCCGGC GCC^GGC
NarI 91 GGCGCC GG^CGCC
NdeII 705 GATC ^GATC
NgoMIV 952 GCCGGC G^CCGGC
NotI 247 GCGGCCGC GC^GGCCGC
PaeR7I 481 CTCGAG C^TCGAG
PmaCI 1084 CACGTG CAC^GTG
PmlI 1084 CACGTG CAC^GTG
PshAI 601 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1273 GAGCTC GAGCT^C
SapI 333 GCTCTTC GCTCTTC(1/4)
Sau3AI 705 GATC ^GATC
ScaI 467 AGTACT AGT^ACT
SfiI 981 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 91 GGCGCC GGC^GCC
SmlI 481 CTCGAG C^T[CT][GA]AG
Tsp509I 1167 AATT ^AATT
XbaI 417 TCTAGA T^CTAGA
XcmI 1475 CCATCGACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 481 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 784 GTCTAC GT^[AC][GT]AC
1369 GTCTAC
BalI 980 TGGCCA TGG^CCA
1472 TGGCCA
BanI 91 GGCGCC G^G[CT][GA]CC
1246 GGTGCC
BcgI 759 CGAGGAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
256 GCAGTCCCCTCG
BsgI 1279 CTGCAC GTGCAG(16/14)
1336 GTGCAG
BsmBI 903 CGTCTC CGTCTC(1/5)
391 GAGACG
EagI 248 CGGCCG C^GGCCG
1331 CGGCCG
EclXI 248 CGGCCG C^GGCCG
1331 CGGCCG
Eco52I 248 CGGCCG C^GGCCG
1331 CGGCCG
EcoNI 201 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1287 CCTGTCTGAGG
HinfI 735 GATTC G^A[ACGT]TC
1305 GATTC
KspI 175 CCGCGG CCGC^GG
554 CCGCGG
MaeIII 619 GTCAC ^GT[ACGT]AC
1394 GTGAC
MboII 877 GAAGA GAAGA(8/7)
335 TCTTC
MluNI 980 TGGCCA TGG^CCA
1472 TGGCCA
MscI 980 TGGCCA TGG^CCA
1472 TGGCCA
PstI 134 CTGCAG CTGCA^G
254 CTGCAG
SacII 175 CCGCGG CCGC^GG
554 CCGCGG
SexAI 614 ACCTGGT A^CC[AT]GGT
668 ACCAGGT
SfaNI 148 GCATC GCATC(5/9)
1430 GCATC
SfcI 134 CTGCAG C^T[GA][CT]AG
254 CTGCAG
StuI 839 AGGCCT AGG^CCT
1238 AGGCCT
TfiI 735 GATTC G^A[AT]TC
1305 GATTC
Tsp45I 619 GTCAC ^GT[GC]AC
1394 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AspHI AvaI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PpuMI
PvuII RsaI Sau96I ScrFI SmaI
StyI TaqI TseI TspRI TthHB8I
XmaCI XmaI