(Circular) (Six-base) MAPSORT of: pFN21AE1963.seq Check: 8671 from: 1 to: 6277 pFN21AE1963 6277 bp With 182 enzymes: SgfI * October 5, 2016 16:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2910 4087 278 Size: 53 83 186 936 1374 1177 2468 Fragments arranged by size: 2468 1374 1177 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2790 3375 3620 1937 Size: 853 585 245 4594 Fragments arranged by size: 4594 853 585 245 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2822 2851 2942 3379 4340 5724 5992 1632 Size: 1190 29 91 437 961 1384 268 1917 Fragments arranged by size: 1917 1384 1190 961 437 268 91 29 AfeI AGC'GCT Cuts at: 2864 2864 Size: 6277 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 3033 5176 6153 1203 Size: 1830 2143 977 1327 Fragments arranged by size: 2143 1830 1327 977 AgeI A'CCGG_T Cuts at: 5108 5108 Size: 6277 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6277 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6277 AlwNI CAG_nnn'CTG Cuts at: 4020 5592 4020 Size: 1572 4705 ApaI G_GGCC'C Cuts at: 2109 2462 2568 2109 Size: 353 106 5818 Fragments arranged by size: 5818 353 106 ApaLI G'TGCA_C Cuts at: 3013 4334 5490 3013 Size: 1321 1156 3800 Fragments arranged by size: 3800 1321 1156 ApoI r'AATT_y Cuts at: 1493 3586 3812 3964 6073 1493 Size: 2093 226 152 2109 1697 Fragments arranged by size: 2109 2093 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4831 160 Size: 872 3799 1606 Fragments arranged by size: 3799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 2101 2276 2347 2376 2486 2659 2973 Size: 73 158 175 71 29 110 173 314 Cuts at: 2973 3602 1870 Size: 629 4545 Fragments arranged by size: 4545 629 314 175 173 158 110 73 71 29 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2096 3251 3598 5053 618 Size: 325 217 627 309 1155 347 1455 1842 Fragments arranged by size: 1842 1455 1155 627 347 325 309 217 BanII G_rGCy'C Cuts at: 1391 2109 2277 2462 2568 3282 3596 1391 Size: 718 168 185 106 714 314 4072 Fragments arranged by size: 4072 718 714 314 185 168 106 BbeI G_GCGC'C Cuts at: 2100 2100 Size: 6277 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2889 928 Size: 971 990 4316 Fragments arranged by size: 4316 990 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2250 2284 2753 2787 4468 Size: 34 50 34 229 34 469 34 1681 Cuts at: 4468 4502 1903 Size: 34 3678 Fragments arranged by size: 3678 1681 469 229 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4169 5379 4169 Size: 1210 5067 BclI T'GATC_A Cuts at: 1548 1548 Size: 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 2118 2993 Size: 107 122 71 631 605 406 40 875 Cuts at: 2993 4888 136 Size: 1895 1525 Fragments arranged by size: 1895 1525 875 631 605 406 122 107 71 40 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 2109 2462 2568 3017 3090 3096 4338 5494 Size: 319 353 106 449 73 6 1242 1156 Cuts at: 5494 1790 Size: 2573 Fragments arranged by size: 2573 1242 1156 449 353 319 106 73 6 BmgBI CAC'GTC Cuts at: 1318 2548 3487 1318 Size: 1230 939 4108 Fragments arranged by size: 4108 1230 939 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3013 4008 4966 Size: 416 53 83 399 24 1596 995 958 Cuts at: 4966 442 Size: 1753 Fragments arranged by size: 1753 1596 995 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2926 2926 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2309 2489 2861 2879 2921 3542 4937 1239 Size: 1070 180 372 18 42 621 1395 2579 Fragments arranged by size: 2579 1395 1070 621 372 180 42 18 Bpu10I CC'TnA_GC Cuts at: 3057 3416 3057 Size: 359 5918 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4402 5267 5565 4402 Size: 865 298 5114 Fragments arranged by size: 5114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2420 4940 882 Size: 617 921 2520 2219 Fragments arranged by size: 2520 2219 921 617 BsaAI yAC'GTr Cuts at: 493 1204 3091 4068 493 Size: 711 1887 977 2702 Fragments arranged by size: 2702 1887 977 711 BsaBI GATnn'nnATC Cuts at: 6271 6271 Size: 6277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 2070 2097 2217 Size: 53 83 186 936 486 51 27 120 Cuts at: 2217 2907 3146 3402 4084 4466 5101 275 Size: 690 239 256 682 382 635 1451 Fragments arranged by size: 1451 936 690 682 635 486 382 256 239 186 120 83 53 51 27 BsaWI w'CCGG_w Cuts at: 4710 5108 5382 5529 5940 4710 Size: 398 274 147 411 5047 Fragments arranged by size: 5047 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2107 2137 1178 Size: 30 165 30 704 30 5318 Fragments arranged by size: 5318 704 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2321 2780 3314 1186 Size: 1135 459 534 4149 Fragments arranged by size: 4149 1135 534 459 BseYI C'CCAG_C Cuts at: 2108 2146 2453 2895 3082 5480 5976 2108 Size: 38 307 442 187 2398 496 2409 Fragments arranged by size: 2409 2398 496 442 307 187 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3269 3362 1163 Size: 573 1533 93 4078 Fragments arranged by size: 4078 1533 573 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2256 3109 3339 3727 4488 5516 Size: 275 186 255 853 230 388 761 1028 Cuts at: 5516 1540 Size: 2301 Fragments arranged by size: 2301 1028 853 761 388 275 255 230 186 BsiHKAI G_wGCw'C Cuts at: 2838 3017 3282 4338 4423 5494 2838 Size: 179 265 1056 85 1071 3621 Fragments arranged by size: 3621 1071 1056 265 179 85 BsmI GAATG_Cn' Cuts at: 3788 3881 3788 Size: 93 6184 BsmBI CGTCTCn'nnnn_ Cuts at: 2390 2914 2390 Size: 524 5753 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2277 2462 2568 2838 3017 3090 Size: 399 319 168 185 106 270 179 73 Cuts at: 3090 3096 3282 3596 4338 4423 5494 1391 Size: 6 186 314 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 319 314 270 186 185 179 168 106 85 73 6 BspEI T'CCGG_A Cuts at: 5940 5940 Size: 6277 BspHI T'CATG_A Cuts at: 4166 5896 4166 Size: 1730 4547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2343 3632 844 Size: 1499 1289 3489 Fragments arranged by size: 3489 1499 1289 BsrBI CCG'CTC Cuts at: 1394 1823 2230 4164 1394 Size: 429 407 1934 3507 Fragments arranged by size: 3507 1934 429 407 BsrDI GCAATG_nn' Cuts at: 65 1248 4772 4946 65 Size: 1183 3524 174 1396 Fragments arranged by size: 3524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2957 4921 5108 1700 Size: 110 1147 1964 187 2869 Fragments arranged by size: 2869 1964 1147 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 3074 4337 5349 3074 Size: 1263 1012 4002 Fragments arranged by size: 4002 1263 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6277 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 2623 1184 Size: 1439 4838 BstYI r'GATC_y Cuts at: 1972 3607 3974 4359 4376 5828 5914 5926 6272 Size: 1635 367 385 17 1452 86 12 346 Cuts at: 6272 1972 Size: 1977 Fragments arranged by size: 1977 1635 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3327 3327 Size: 6277 BtgI C'CryG_G Cuts at: 513 1065 2126 2180 2559 513 Size: 552 1061 54 379 4231 Fragments arranged by size: 4231 1061 552 379 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 3286 3793 4587 4607 3286 Size: 507 794 20 4956 Fragments arranged by size: 4956 794 507 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 3581 3581 Size: 6277 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3367 5284 809 Size: 507 2051 1917 1802 Fragments arranged by size: 2051 1917 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2508 2955 Size: 54 1425 50 275 18 423 255 447 Cuts at: 2955 2985 3102 3336 3477 3724 4613 6242 8 Size: 30 117 234 141 247 889 1629 43 Fragments arranged by size: 1629 1425 889 447 423 275 255 247 234 141 117 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2253 3336 3724 1537 Size: 275 441 1083 388 4090 Fragments arranged by size: 4090 1083 441 388 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2345 4207 1353 Size: 992 1862 3423 Fragments arranged by size: 3423 1862 992 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2959 3017 4864 5238 5384 387 Size: 1064 138 1370 58 1847 374 146 1280 Fragments arranged by size: 1847 1370 1280 1064 374 146 138 58 EcoICRI GAG'CTC Cuts at: 3280 3280 Size: 6277 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2309 2489 2822 2851 2861 2879 2921 Size: 393 677 180 333 29 10 18 42 Cuts at: 2921 2942 3379 3542 4340 4937 5724 5992 1239 Size: 21 437 163 798 597 787 268 1524 Fragments arranged by size: 1524 798 787 677 597 437 393 333 268 180 163 42 29 21 18 10 EcoNI CCTnn'n_nnAGG Cuts at: 2210 3296 2210 Size: 1086 5191 EcoO109I rG'GnC_Cy Cuts at: 2105 2175 2281 2458 2459 2503 2564 2572 2806 Size: 70 106 177 1 44 61 8 234 Cuts at: 2806 3156 2105 Size: 350 5226 Fragments arranged by size: 5226 350 234 177 106 70 61 44 8 1 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FseI GG_CCGG'CC Cuts at: 2961 2961 Size: 6277 FspI TGC'GCA Cuts at: 2552 4783 2552 Size: 2231 4046 HaeII r_GCGC'y Cuts at: 1678 2100 2372 2866 3445 3517 5424 1678 Size: 422 272 494 579 72 1907 2531 Fragments arranged by size: 2531 1907 579 494 422 272 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3167 3199 4918 4950 4992 Size: 32 135 32 1595 32 1719 32 42 Cuts at: 4992 5024 1373 Size: 32 2626 Fragments arranged by size: 2626 1719 1595 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3621 3867 669 Size: 988 281 1683 246 3079 Fragments arranged by size: 3079 1683 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6277 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KasI G'GCGC_C Cuts at: 2096 2096 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3876 3876 Size: 6277 MluI A'CGCG_T Cuts at: 3033 6153 3033 Size: 3120 3157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5391 5575 1305 Size: 4086 184 2007 Fragments arranged by size: 4086 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2987 3479 10 Size: 54 1425 343 1155 492 2808 Fragments arranged by size: 2808 1425 1155 492 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3485 4235 4594 4753 518 Size: 648 2319 750 359 159 2042 Fragments arranged by size: 2319 2042 750 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2150 2182 2252 2258 2561 2871 2899 2944 Size: 544 32 70 6 303 310 28 45 Cuts at: 2944 3693 4370 5518 5763 1606 Size: 749 677 1148 245 2120 Fragments arranged by size: 2120 1148 749 677 544 310 303 245 70 45 32 28 6 NaeI GCC'GGC Cuts at: 2959 2959 Size: 6277 NarI GG'CG_CC Cuts at: 2097 2097 Size: 6277 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5725 NdeI CA'TA_TG Cuts at: 387 387 Size: 6277 NgoMIV G'CCGG_C Cuts at: 2957 2957 Size: 6277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 2253 3724 2253 Size: 1471 4806 NspI r_CATG'y Cuts at: 1132 1229 3635 5180 1132 Size: 97 2406 1545 2229 Fragments arranged by size: 2406 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3151 1348 Size: 1803 4474 PciI A'CATG_T Cuts at: 5176 5176 Size: 6277 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6277 PfoI T'CCnGG_A Cuts at: 3531 3531 Size: 6277 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PmlI CAC'GTG Cuts at: 3091 3091 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2175 2281 2503 2572 2175 Size: 106 222 69 5880 Fragments arranged by size: 5880 222 106 69 PshAI GACnn'nnGTC Cuts at: 1613 2610 1613 Size: 997 5280 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PspOMI G'GGCC_C Cuts at: 2105 2458 2564 2105 Size: 353 106 5818 Fragments arranged by size: 5818 353 106 PspXI vC'TCGA_Gb Cuts at: 2486 2486 Size: 6277 PstI C_TGCA'G Cuts at: 830 2143 2263 3629 830 Size: 1313 120 1366 3478 Fragments arranged by size: 3478 1366 1313 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2150 2899 2944 2150 Size: 749 45 5483 Fragments arranged by size: 5483 749 45 SacI G_AGCT'C Cuts at: 3282 3282 Size: 6277 SacII CC_GC'GG Cuts at: 2183 2562 2183 Size: 379 5898 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SanDI GG'GwC_CC Cuts at: 2281 2281 Size: 6277 SapI GCTCTTCn'nnn_ Cuts at: 2345 2345 Size: 6277 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 2474 4525 6237 2474 Size: 2051 1712 2514 Fragments arranged by size: 2514 2051 1712 SexAI A'CCwGG_T Cuts at: 2619 2673 2619 Size: 54 6223 SfcI C'TryA_G Cuts at: 826 1045 2139 2259 3625 4760 5441 5632 826 Size: 219 1094 120 1366 1135 681 191 1471 Fragments arranged by size: 1471 1366 1135 1094 681 219 191 120 SfiI GGCCn_nnn'nGGCC Cuts at: 2993 2993 Size: 6277 SfoI GGC'GCC Cuts at: 2098 2098 Size: 6277 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 2103 2278 2661 2975 3604 1872 Size: 231 175 383 314 629 4545 Fragments arranged by size: 4545 629 383 314 231 175 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 4381 5282 5544 820 Size: 197 926 543 1895 901 262 1553 Fragments arranged by size: 1895 1553 926 901 543 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 4201 5 Size: 47 4149 2081 Fragments arranged by size: 4149 2081 47 StuI AGG'CCT Cuts at: 1517 2846 3245 1517 Size: 1329 399 4549 Fragments arranged by size: 4549 1329 399 StyI C'CwwG_G Cuts at: 513 1065 2037 2171 2568 3405 3716 513 Size: 552 972 134 397 837 311 3074 Fragments arranged by size: 3074 972 837 552 397 311 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3032 4304 4321 4474 4659 5104 325 Size: 1255 1452 1272 17 153 185 445 1498 Fragments arranged by size: 1498 1452 1272 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 2721 2754 Size: 274 80 33 51 1443 495 249 33 Cuts at: 2754 3192 4523 6235 96 Size: 438 1331 1712 138 Fragments arranged by size: 1712 1443 1331 495 438 274 249 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2107 2139 6237 6269 2107 Size: 32 4098 32 2115 Fragments arranged by size: 4098 2115 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6277 XbaI T'CTAG_A Cuts at: 2422 3613 2422 Size: 1191 5086 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3487 3487 Size: 6277 XhoI C'TCGA_G Cuts at: 1943 2486 1943 Size: 543 5734 XmaI C'CCGG_G Cuts at: 1870 2101 2276 2659 2973 3602 1870 Size: 231 175 383 314 629 4545 Fragments arranged by size: 4545 629 383 314 231 175 XmnI GAAnn'nnTTC Cuts at: 4406 4406 Size: 6277 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2908 4085 276 Size: 53 83 186 936 1374 1177 2468 Fragments arranged by size: 2468 1374 1177 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FalI FspAI NruI NsiI PacI PsrI RsrII SrfI SwaI