################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 83 GACGTC GACGT^C AcsI 244 GAATTT [GA]^AATT[CT] AcyI 83 GACGTC G[GA]^CG[CT]C AfaI 220 GTAC GT^AC AlwI 415 GATCC GGATC(4/5) ApoI 244 GAATTT [GA]^AATT[CT] BcgI 78 GCAGCGACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 467 GGATAC GTATCC(6/5) BcnI 8 CCCGG CC^[GC]GG BsaHI 83 GACGTC G[GA]^CG[CT]C BseRI 164 CTCCTC GAGGAG(10/8) BsiEI 49 CGGCCG CG[GA][CT]^CG BsmFI 6 GTCCC GGGAC(10/14) BsmI 355 GAATGC GAATGC(1/-1) BsrDI 227 GCAATG GCAATG(2/0) BssHII 41 GCGCGC G^CGCGC BstEII 334 GGTCACC G^GT[ACGT]ACC BstF5I 278 GGATG GGATG(2/0) BstPI 334 GGTCACC G^GT[ACGT]ACC BstYI 414 AGATCC [GA]^GATC[CT] DpnII 415 GATC ^GATC EaeI 49 CGGCCG [CT]^GGCC[GA] EagI 49 CGGCCG C^GGCCG EclXI 49 CGGCCG C^GGCCG Eco52I 49 CGGCCG C^GGCCG EcoO65I 334 GGTCACC G^GT[ACGT]ACC FauI 37 GCGGG CCCGC(4/6) FokI 278 GGATG GGATG(9/13) HgaI 112 GACGC GACGC(5/10) Hin1I 83 GACGTC G[GA]^CG[CT]C MboI 415 GATC ^GATC MflI 414 AGATCC [GA]^GATC[CT] MseI 478 TTAA T^TAA MspA1I 121 CAGCTG C[AC]G^C[GT]G NciI 8 CCCGG CC^[GC]GG NdeII 415 GATC ^GATC NotI 48 GCGGCCGC GC^GGCCGC PvuII 121 CAGCTG CAG^CTG RsaI 220 GTAC GT^AC Sau3AI 415 GATC ^GATC SfaNI 279 GATGC GCATC(5/9) SmlI 133 CTCAAG C^T[CT][GA]AG TfiI 496 GATTC G^A[AT]TC Tru9I 478 TTAA T^TAA Tsp509I 245 AATT ^AATT XcmI 239 CCAGGGAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 414 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BsmAI 190 GAGAC GTCTC(1/5) 437 GAGAC BstAPI 69 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 117 GCAGCAGCTGC Cac8I 37 GCGGGC GC[ACGT]^[ACGT]GC 41 GCGCGC HaeIII 11 GGCC GG^CC 50 GGCC HapII 9 CCGG C^CGG 64 CCGG HpaII 9 CCGG C^CGG 64 CCGG HphI 346 GGTGA GGTGA(8/7) 336 TCACC MspI 9 CCGG C^CGG 64 CCGG PleI 655 GACTC GAGTC(4/5) 145 GAGTC PstI 76 CTGCAG CTGCA^G 124 CTGCAG SfcI 76 CTGCAG C^T[GA][CT]AG 124 CTGCAG Tsp45I 335 GTCAC ^GT[GC]AC 347 GTGAC TspRI 284 CACTG CA[GC]TG[ACGT][ACGT]^ 310 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AccII AciI AluI AlwNI BbvI BpmI BsaJI BsiYI BslI BsrI BssKI BstNI BstUI CfoI DdeI EcoRII Fnu4HI HhaI HinP1I HinfI ItaI MaeII MaeIII MboII MnlI MvaI MvnI MwoI NlaIII ScrFI TseI