Restriction map for pFN21AB6784
 (Circular) (Six-base) MAPSORT of: pFN21AB6784.seq  Check: 1928  from: 1  to: 5164



pFN21AB6784  5164 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 14:40  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2974     278 
   Size:         53      83     186     936    1438    2468

  Fragments arranged by size: 

                2468    1438     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2507    1937 
   Size:        570    4594
 
Acc65I G'GTAC_C

Cuts at:   2485    2485 
   Size:       5164
 
AclI AA'CG_TT

Cuts at:   1563    3291    3664    1563 
   Size:       1728     373    3063

  Fragments arranged by size: 

                3063    1728     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3227    4611    4879    1632 
   Size:       1595    1384     268    1917

  Fragments arranged by size: 

                1917    1595    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4967
 
AflIII A'CryG_T

Cuts at:   1203    4063    5040    1203 
   Size:       2860     977    1327

  Fragments arranged by size: 

                2860    1327     977
 
AgeI A'CCGG_T

Cuts at:   3995    3995 
   Size:       5164
 
AhdI GACnn_n'nnGTC

Cuts at:   3893    3893 
   Size:       5164
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5164
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5164
 
AlwNI CAG_nnn'CTG

Cuts at:   2907    4479    2907 
   Size:       1572    3592
 
ApaLI G'TGCA_C

Cuts at:   2146    3221    4377    2146 
   Size:       1075    1156    2933

  Fragments arranged by size: 

                2933    1156    1075
 
ApoI r'AATT_y

Cuts at:   1493    2365    2473    2699    2851    4960    1493 
   Size:        872     108     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     872     226     152     108
 
AseI AT'TA_AT

Cuts at:    160    1032    3718     160 
   Size:        872    2686    1606

  Fragments arranged by size: 

                2686    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5164
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2489    1870 
   Size:         73     546    4545

  Fragments arranged by size: 

                4545     546      73
 
BamHI G'GATC_C

Cuts at:   2494    2861    2494 
   Size:        367    4797
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2485    3940     618 
   Size:        325     217     627     698    1455    1842

  Fragments arranged by size: 

                1842    1455     698     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2483    1391 
   Size:       1092    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2144     928 
   Size:        971     245    3948

  Fragments arranged by size: 

                3948     971     245
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3355    3389    1903 
   Size:         34    1418      34    3678

  Fragments arranged by size: 

                3678    1418      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3056    4266    3056 
   Size:       1210    3954
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5164
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2358    3775     136 
   Size:        107     122      71     631     605     686    1417    1525

  Fragments arranged by size: 

                1525    1417     686     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5159    5159 
   Size:       5164
 
BlpI GC'TnA_GC

Cuts at:   2581    2581 
   Size:       5164
 
Bme1580I G_kGCm'C

Cuts at:   1790    2150    3225    4381    1790 
   Size:        360    1075    1156    2573

  Fragments arranged by size: 

                2573    1156    1075     360
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5164
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2895    3853     442 
   Size:        416      53      83     399      24    1478     958    1753

  Fragments arranged by size: 

                1753    1478     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    2051    1056 
   Size:        995    4169
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3824    1239 
   Size:       2585    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3289    4154    4452    3289 
   Size:        865     298    4001

  Fragments arranged by size: 

                4001     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2015    3827     882 
   Size:        617     516    1812    2219

  Fragments arranged by size: 

                2219    1812     617     516
 
BsaAI yAC'GTr

Cuts at:    493    1204    2955     493 
   Size:        711    1751    2702

  Fragments arranged by size: 

                2702    1751     711
 
BsaBI GATnn'nnATC

Cuts at:   5158    5158 
   Size:       5164
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2971    3353    3988     275 
   Size:         53      83     186     936    1438     382     635    1451

  Fragments arranged by size: 

                1451    1438     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3597    3995    4269    4416    4827    3597 
   Size:        398     274     147     411    3934

  Fragments arranged by size: 

                3934     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4939

  Fragments arranged by size: 

                4939     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5164
 
BseYI C'CCAG_C

Cuts at:   4367    4863    4367 
   Size:        496    4668
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2268    1163 
   Size:        573     532    4059

  Fragments arranged by size: 

                4059     573     532
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2614    3375    4403    1540 
   Size:        275     186     613     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     613     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2150    2232    3225    3310    4381    2150 
   Size:         82     993      85    1071    2933

  Fragments arranged by size: 

                2933    1071     993      85      82
 
BsmI GAATG_Cn'

Cuts at:   2675    2768    2675 
   Size:         93    5071
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2150    2232    2483    3225    3310    4381    1391 
   Size:        399     360      82     251     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     360     251      85      82
            
 
BspEI T'CCGG_A

Cuts at:   4827    4827 
   Size:       5164
 
BspHI T'CATG_A

Cuts at:   2433    3053    4783    2433 
   Size:        620    1730    2814

  Fragments arranged by size: 

                2814    1730     620
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2519     844 
   Size:       1675    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3051    1394 
   Size:        429    1228    3507

  Fragments arranged by size: 

                3507    1228     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3659    3833      65 
   Size:       1183    2411     174    1396

  Fragments arranged by size: 

                2411    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2057    3808    3995    1700 
   Size:        110     247    1751     187    2869

  Fragments arranged by size: 

                2869    1751     247     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5164
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5164
 
BssSI C'ACGA_G

Cuts at:   3224    4236    3224 
   Size:       1012    4152
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5164
 
BstBI TT'CG_AA

Cuts at:   3968    3968 
   Size:       5164
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5164
 
BstYI r'GATC_y

Cuts at:   1972    2494    2861    3246    3263    4715    4801    4813    5159 
   Size:        522     367     385      17    1452      86      12     346

Cuts at:   5159    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     522     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2071     513 
   Size:        552     939      67    3606

  Fragments arranged by size: 

                3606     939     552      67
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4425

  Fragments arranged by size: 

                4425     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2680    3474    3494    2680 
   Size:        794      20    4350

  Fragments arranged by size: 

                4350     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5164
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5129
 
DraI TTT'AAA

Cuts at:   2468    2468 
   Size:       5164
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4171     809 
   Size:        507    2855    1802

  Fragments arranged by size: 

                2855    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2611    3500    5129 
   Size:         54    1425      50     275      18     781     889    1629

Cuts at:   5129       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     781     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2611    1537 
   Size:        275     799    4090

  Fragments arranged by size: 

                4090     799     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3094    1353 
   Size:       1741    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3751    4125    4271     387 
   Size:       1064     138    2162     374     146    1280

  Fragments arranged by size: 

                2162    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3227    3824    4611    4879    1239 
   Size:        393    1595     597     787     268    1524

  Fragments arranged by size: 

                1595    1524     787     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2212    2212 
   Size:       5164
 
EcoO109I rG'GnC_Cy

Cuts at:   2350    2350 
   Size:       5164
 
EcoRI G'AATT_C

Cuts at:   2365    2473    2851    2365 
   Size:        108     378    4678

  Fragments arranged by size: 

                4678     378     108
 
EcoRV GAT'ATC

Cuts at:   2089    2089 
   Size:       5164
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2224    2224 
   Size:       5164
 
FspI TGC'GCA

Cuts at:   3670    3670 
   Size:       5164
 
HaeII r_GCGC'y

Cuts at:   1678    4311    1678 
   Size:       2633    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3805    3837    3879    3911    1373 
   Size:         32     135      32    2233      32      42      32    2626

  Fragments arranged by size: 

                2626    2233     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2157    2508    2754     669 
   Size:        988     281     219     351     246    3079

  Fragments arranged by size: 

                3079     988     351     281     246     219
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5164
 
HpaI GTT'AAC

Cuts at:   2754    2754 
   Size:       5164
 
KpnI G_GTAC'C

Cuts at:   2489    2489 
   Size:       5164
 
MfeI C'AATT_G

Cuts at:   2763    2763 
   Size:       5164
 
MluI A'CGCG_T

Cuts at:   5040    5040 
   Size:       5164
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2382    4278    4462    1305 
   Size:       1077    1896     184    2007

  Fragments arranged by size: 

                2007    1896    1077     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3342

  Fragments arranged by size: 

                3342    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3122    3481    3640     518 
   Size:        648    1956     359     159    2042

  Fragments arranged by size: 

                2042    1956     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2311    2580    3257    4405    4650    1606 
   Size:        705     269     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     705     677     269     245
 
NaeI GCC'GGC

Cuts at:   2059    2059 
   Size:       5164
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3673

  Fragments arranged by size: 

                3673     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5164
 
NgoMIV G'CCGG_C

Cuts at:   2057    2057 
   Size:       5164
 
NheI G'CTAG_C

Cuts at:   1052    2047    1052 
   Size:        995    4169
 
NotI GC'GGCC_GC

Cuts at:   2611    2611 
   Size:       5164
 
NspI r_CATG'y

Cuts at:   1132    1229    2055    2522    4067    1132 
   Size:         97     826     467    1545    2229

  Fragments arranged by size: 

                2229    1545     826     467      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5164
 
PciI A'CATG_T

Cuts at:   4063    4063 
   Size:       5164
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5164
 
PmeI GTTT'AAAC

Cuts at:   2468    2468 
   Size:       5164
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3233    3265    4746    4778    3233 
   Size:         32    1481      32    3619

  Fragments arranged by size: 

                3619    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5164
 
PsiI TTA'TAA

Cuts at:   2734    2734 
   Size:       5164
 
PstI C_TGCA'G

Cuts at:    830    2216    2516     830 
   Size:       1386     300    3478

  Fragments arranged by size: 

                3478    1386     300
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5164
 
PvuII CAG'CTG

Cuts at:   2311    2311 
   Size:       5164
 
SalI G'TCGA_C

Cuts at:   1936    2506    1936 
   Size:        570    4594
 
SbfI CC_TGCA'GG

Cuts at:   2516    2516 
   Size:       5164
 
ScaI AGT'ACT

Cuts at:   3412    5124    3412 
   Size:       1712    3452
 
SfcI C'TryA_G

Cuts at:    826    1045    2212    2242    2512    3647    4328    4519     826 
   Size:        219    1167      30     270    1135     681     191    1471

  Fragments arranged by size: 

                1471    1167    1135     681     270     219     191      30
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5164
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5164
 
SmaI CCC'GGG

Cuts at:   1872    2491    1872 
   Size:        619    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3268    4169    4431     820 
   Size:        197     926    1325     901     262    1553

  Fragments arranged by size: 

                1553    1325     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2955     493 
   Size:       2462    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5164
 
SphI G_CATG'C

Cuts at:   1132    2522    1132 
   Size:       1390    3774
 
SspI AAT'ATT

Cuts at:      5      52    2447    3088       5 
   Size:         47    2395     641    2081

  Fragments arranged by size: 

                2395    2081     641      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5164
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2603     513 
   Size:        552     939     599    3074

  Fragments arranged by size: 

                3074     939     599     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2264    3191    3208    3361    3546    3991     325 
   Size:       1255     684     927      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     927     684     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3410    5122      96 
   Size:        274      80      33      51    1443    1433    1712     138

  Fragments arranged by size: 

                1712    1443    1433     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2277    2309    5124    5156    2277 
   Size:         32    2815      32    2285

  Fragments arranged by size: 

                2815    2285      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5164
 
XbaI T'CTAG_A

Cuts at:   2500    2500 
   Size:       5164
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5164
 
XmaI C'CCGG_G

Cuts at:   1870    2489    1870 
   Size:        619    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3293    3293 
   Size:       5164
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2972     276 
   Size:         53      83     186     936    1438    2468

  Fragments arranged by size: 

                2468    1438     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI     FseI    FspAI     KasI     NarI     NruI
     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
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