################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII AccII AccIII AciI AclI AflII AgeI AhdI AlwI AlwNI ApaI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaJI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DpnII DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeIII HincII HindII HindIII HpaI KasI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI StyI Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 76 enzymes) # ################################### Acc65I 136 GGTACC G^GTACC AccI 115 GTAGAC GT^[AC][GT]AC AcyI 3 GGCGTC G[GA]^CG[CT]C AfaI 137 GTAC GT^AC AflIII 400 ACATGT A^C[GA][CT]GT AluI 352 AGCT AG^CT Alw44I 128 GTGCAC G^TGCAC AocI 205 CCTCAGG CC^T[ACGT]AGG Aor51HI 303 AGCGCT AGC^GCT ApaLI 128 GTGCAC G^TGCAC Asp718I 136 GGTACC G^GTACC AspHI 128 GTGCAC G[AT]GC[AT]^C AvaII 296 GGTCC G^G[AT]CC BanI 136 GGTACC G^G[CT][GA]CC BbsI 289 GAAGAC GAAGAC(2/6) BbvI 353 GCTGC GCAGC(8/12) BmyI 128 GTGCAC G[AGT]GC[ACT]^C BpuAI 289 GAAGAC GAAGAC(2/6) BsaHI 3 GGCGTC G[GA]^CG[CT]C BsaWI 375 ACCGGA [AT]^CCGG[AT] BseRI 203 CTCCTC GAGGAG(10/8) BsiHKAI 128 GTGCAC G[AT]GC[AT]^C BsiYI 55 CCACCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 55 CCACCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 366 GAGAC GTCTC(1/5) BsmBI 366 GAGACG CGTCTC(1/5) BsmFI 297 GTCCC GGGAC(10/14) Bsp1286I 128 GTGCAC G[AGT]GC[ACT]^C BspHI 438 TCATGA T^CATGA BspLU11I 400 ACATGT A^CATGT BsrDI 257 GCAATG GCAATG(2/0) BstF5I 241 CATCC GGATG(2/0) Bsu36I 205 CCTCAGG CC^T[ACGT]AGG Cac8I 341 GCATGC GC[ACGT]^[ACGT]GC CfoI 304 GCGC GCG^C Cfr13I 296 GGTCC G^G[ACGT]CC DraI 172 TTTAAA TTT^AAA EarI 81 GAAGAG CTCTTC(1/4) Eco47III 303 AGCGCT AGC^GCT Eco81I 205 CCTCAGG CC^T[ACGT]AGG EcoRV 188 GATATC GAT^ATC Fnu4HI 353 GCTGC GC^[ACGT]GC FokI 241 CATCC GGATG(9/13) HaeII 303 AGCGCT [GA]GCGC^[CT] HapII 376 CCGG C^CGG HgaI 4 GCGTC GACGC(5/10) HhaI 304 GCGC GCG^C Hin1I 3 GGCGTC G[GA]^CG[CT]C HinP1I 304 GCGC G^CGC HpaII 376 CCGG C^CGG HphI 223 GGTGA GGTGA(8/7) ItaI 353 GCTGC GC^[ACGT]GC KpnI 136 GGTACC GGTAC^C Ksp632I 81 GAAGAG CTCTTC(1/4) MaeIII 404 GTAAC ^GT[ACGT]AC MslI 241 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 351 CAGCTG C[AC]G^C[GT]G MspI 376 CCGG C^CGG MwoI 332 GCATTATTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 55 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 199 GACTC GAGTC(4/5) PvuII 351 CAGCTG CAG^CTG RcaI 438 TCATGA T^CATGA RsaI 137 GTAC GT^AC Sau96I 296 GGTCC G^G[ACGT]CC SexAI 132 ACCAGGT A^CC[AT]GGT SfcI 22 CTACAG C^T[GA][CT]AG SphI 341 GCATGC GCATG^C SspI 229 AATATT AAT^ATT SwaI 171 ATTTAAAT ATTT^AAAT TaqI 186 TCGA T^CGA TfiI 411 GAATC G^A[AT]TC TseI 353 GCTGC G^C[AT]GC TspRI 106 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 186 TCGA T^CGA Van91I 55 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### Asp700I 154 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 226 GAAAATATTC BsrI 220 ACTGG ACTGG(1/-1) 293 ACTGG BssKI 61 CCTGG ^CC[ACGT]GG 133 CCAGG BstNI 61 CCTGG CC^[AT]GG 133 CCAGG EcoRII 61 CCTGG ^CC[AT]GG 133 CCAGG HinfI 199 GACTC G^A[ACGT]TC 411 GAATC MboII 81 GAAGA GAAGA(8/7) 289 GAAGA MseI 73 TTAA T^TAA 173 TTAA MvaI 61 CCTGG CC^[AT]GG 133 CCAGG NlaIV 136 GGTACC GG[ACGT]^[ACGT]CC 296 GGTCCC NspI 341 GCATGC [GA]CATG^[CT] 400 ACATGT ScrFI 61 CCTGG CC^[ACGT]GG 133 CCAGG Tru9I 73 TTAA T^TAA 173 TTAA XmnI 154 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 226 GAAAATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### AcsI ApoI DdeI MnlI NlaIII Tsp509I