Restriction map for
pFN21AE2398
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AhdI Alw44I AlwNI AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvrII BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BseRI BsiWI BsmAI BsmBI BsoBI
BspDI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
DraIII DrdI Eam1105I EarI Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KasI KpnI
Ksp632I MaeIII MamI MfeI MluI
MunI NarI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI StuI SwaI Tsp45I
Tsp509I TspRI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 766 GACGTC GACGT^C
AgeI 533 ACCGGT A^CCGGT
Aor51HI 506 AGCGCT AGC^GCT
ApaI 734 GGGCCC GGGCC^C
AviII 8 TGCGCA TGC^GCA
BamHI 167 GGATCC G^GATCC
BbsI 207 GAAGAC GAAGAC(2/6)
BclI 329 TGATCA T^GATCA
BpuAI 207 GAAGAC GAAGAC(2/6)
BsgI 338 GTGCAG GTGCAG(16/14)
BsmI 215 GAATGC GAATGC(1/-1)
Bsp1407I 518 TGTACA T^GTACA
BspHI 326 TCATGA T^CATGA
BsrGI 518 TGTACA T^GTACA
BstAPI 504 GCAGCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 286 CGGACCG CG^G[AT]CCG
DraI 393 TTTAAA TTT^AAA
DsaI 743 CCGCGG C^C[GA][CT]GG
Eco47III 506 AGCGCT AGC^GCT
EcoT14I 414 CCAAGG C^C[AT][AT]GG
FbaI 329 TGATCA T^GATCA
FspI 8 TGCGCA TGC^GCA
KspI 743 CCGCGG CCGC^GG
MseI 394 TTAA T^TAA
NotI 745 GCGGCCGC GC^GGCCGC
PinAI 533 ACCGGT A^CCGGT
PstI 664 CTGCAG CTGCA^G
RcaI 326 TCATGA T^CATGA
RsrII 286 CGGACCG CG^G[AT]CCG
SacI 1004 GAGCTC GAGCT^C
SacII 743 CCGCGG CCGC^GG
SbfI 663 CCTGCAGG CCTGCA^GG
ScaI 971 AGTACT AGT^ACT
SfcI 664 CTGCAG C^T[GA][CT]AG
SmlI 271 CTCAAG C^T[CT][GA]AG
Sse8387I 663 CCTGCAGG CCTGCA^GG
SspBI 518 TGTACA T^GTACA
StyI 414 CCAAGG C^C[AT][AT]GG
TaqI 1002 TCGA T^CGA
TfiI 483 GAATC G^A[AT]TC
Tru9I 394 TTAA T^TAA
TthHB8I 1002 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 554 TCCGGA T^CCGGA
857 TCCGGA
AspHI 175 GAGCAC G[AT]GC[AT]^C
1004 GAGCTC
BfaI 122 CTAG C^TAG
430 CTAG
BglI 62 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
881 GCCTGGCCGGC
BpmI 172 CTGGAG CTGGAG(16/14)
300 CTGGAG
BseAI 554 TCCGGA T^CCGGA
857 TCCGGA
BsiEI 746 CGGCCG CG[GA][CT]^CG
828 CGGCCG
BsiHKAI 175 GAGCAC G[AT]GC[AT]^C
1004 GAGCTC
BsmFI 56 GGGAC GGGAC(10/14)
705 GTCCC
BspEI 554 TCCGGA T^CCGGA
857 TCCGGA
BspMI 113 ACCTGC ACCTGC(4/8)
672 GCAGGT
BsrI 246 CCAGT ACTGG(1/-1)
633 CCAGT
BstYI 167 GGATCC [GA]^GATC[CT]
234 AGATCC
DdeI 717 CTCAG C^T[ACGT]AG
791 CTCAG
EagI 746 CGGCCG C^GGCCG
828 CGGCCG
EclXI 746 CGGCCG C^GGCCG
828 CGGCCG
Eco52I 746 CGGCCG C^GGCCG
828 CGGCCG
HgaI 85 GACGC GACGC(5/10)
317 GCGTC
HincII 319 GTCAAC GT[CT]^[GA]AC
676 GTCAAC
HindII 319 GTCAAC GT[CT]^[GA]AC
676 GTCAAC
MaeI 122 CTAG C^TAG
430 CTAG
MboII 207 GAAGA GAAGA(8/7)
965 TCTTC
MflI 167 GGATCC [GA]^GATC[CT]
234 AGATCC
MroI 554 TCCGGA T^CCGGA
857 TCCGGA
MslI 138 CACCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
327 CATGATCATG
NaeI 830 GCCGGC GCC^GGC
886 GCCGGC
NgoMIV 830 GCCGGC G^CCGGC
886 GCCGGC
PflMI 30 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
941 CCATTTCTTGG
PleI 199 GACTC GAGTC(4/5)
228 GAGTC
Van91I 30 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
941 CCATTTCTTGG
XcmI 752 CCACGACCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
983 CCAAGCGCCAGCTGG
XhoII 167 GGATCC [GA]^GATC[CT]
234 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BalI BanII BbvI
BcnI BmyI BsaHI BsaJI BsaWI
BsiYI BslI Bsp1286I BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI