(Circular) (Six-base) MAPSORT of: pFN21AE2398.seq Check: 6125 from: 1 to: 5719 pFN21AE2398 5719 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2775 3529 278 Size: 53 83 186 936 1239 754 2468 Fragments arranged by size: 2468 1239 936 754 186 83 53 AccI GT'mk_AC Cuts at: 1937 3062 1937 Size: 1125 4594 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3782 5166 5434 1632 Size: 2150 1384 268 1917 Fragments arranged by size: 2150 1917 1384 268 AfeI AGC'GCT Cuts at: 2513 2513 Size: 5719 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 4618 5595 1203 Size: 3415 977 1327 Fragments arranged by size: 3415 1327 977 AgeI A'CCGG_T Cuts at: 2538 4550 2538 Size: 2012 3707 AhdI GACnn_n'nnGTC Cuts at: 4448 4448 Size: 5719 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2569 1184 Size: 1385 4334 AlwNI CAG_nnn'CTG Cuts at: 3462 5034 3462 Size: 1572 4147 ApaI G_GGCC'C Cuts at: 2743 2743 Size: 5719 ApaLI G'TGCA_C Cuts at: 3776 4932 3776 Size: 1156 4563 ApoI r'AATT_y Cuts at: 1493 3028 3254 3406 5515 1493 Size: 1535 226 152 2109 1697 Fragments arranged by size: 2109 1697 1535 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 3044 1870 Size: 73 1101 4545 Fragments arranged by size: 4545 1101 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2245 2278 2245 Size: 33 5686 BamHI G'GATC_C Cuts at: 2172 3049 3416 2172 Size: 877 367 4475 Fragments arranged by size: 4475 877 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3040 4495 618 Size: 325 217 627 1253 1455 1842 Fragments arranged by size: 1842 1455 1253 627 325 217 BanII G_rGCy'C Cuts at: 1391 2275 2743 3013 3038 1391 Size: 884 468 270 25 4072 Fragments arranged by size: 4072 884 468 270 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2219 928 Size: 971 320 4428 Fragments arranged by size: 4428 971 320 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3910 3944 1903 Size: 34 1973 34 3678 Fragments arranged by size: 3678 1973 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 2334 1548 Size: 786 4933 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2073 2892 4330 Size: 107 122 71 631 605 401 819 1438 Cuts at: 4330 136 Size: 1525 Fragments arranged by size: 1525 1438 819 631 605 401 122 107 71 BglII A'GATC_T Cuts at: 5714 5714 Size: 5719 BlpI GC'TnA_GC Cuts at: 3136 3136 Size: 5719 Bme1580I G_kGCm'C Cuts at: 1790 2743 3780 4936 1790 Size: 953 1037 1156 2573 Fragments arranged by size: 2573 1156 1037 953 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5719 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2245 3450 4408 Size: 416 53 83 399 24 828 1205 958 Cuts at: 4408 442 Size: 1753 Fragments arranged by size: 1753 1205 958 828 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2326 4379 1239 Size: 959 128 2053 2579 Fragments arranged by size: 2579 2053 959 128 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2261 3844 4709 5007 2261 Size: 1583 865 298 2973 Fragments arranged by size: 2973 1583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4382 882 Size: 617 2883 2219 Fragments arranged by size: 2883 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3510 493 Size: 711 2306 2702 Fragments arranged by size: 2702 2306 711 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 2322 2772 3526 Size: 53 83 186 936 558 231 450 754 Cuts at: 3526 3908 4543 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 754 635 558 450 382 231 186 83 53 BsaWI w'CCGG_w Cuts at: 2538 2559 2598 2691 2862 4152 4550 4824 4971 Size: 21 39 93 171 1290 398 274 147 Cuts at: 4971 5382 2538 Size: 411 2875 Fragments arranged by size: 2875 1290 411 398 274 171 147 93 39 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5494 Fragments arranged by size: 5494 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5719 BseYI C'CCAG_C Cuts at: 2450 4922 5418 2450 Size: 2472 496 2751 Fragments arranged by size: 2751 2472 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2364 1163 Size: 573 628 4518 Fragments arranged by size: 4518 628 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2754 2836 3169 3930 4958 1540 Size: 275 186 753 82 333 761 1028 2301 Fragments arranged by size: 2301 1028 761 753 333 275 186 82 BsiHKAI G_wGCw'C Cuts at: 2184 3013 3780 3865 4936 2184 Size: 829 767 85 1071 2967 Fragments arranged by size: 2967 1071 829 767 85 BsmI GAATG_Cn' Cuts at: 2226 3230 3323 2226 Size: 1004 93 4622 Fragments arranged by size: 4622 1004 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2275 2743 3013 3038 3780 3865 Size: 399 394 91 468 270 25 742 85 Cuts at: 3865 4936 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 468 399 394 270 91 85 25 BspEI T'CCGG_A Cuts at: 2559 2862 5382 2559 Size: 303 2520 2896 Fragments arranged by size: 2896 2520 303 BspHI T'CATG_A Cuts at: 2331 3608 5338 2331 Size: 1277 1730 2712 Fragments arranged by size: 2712 1730 1277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 2668 3074 844 Size: 1283 541 406 3489 Fragments arranged by size: 3489 1283 541 406 BsrBI CCG'CTC Cuts at: 1394 1823 3606 1394 Size: 429 1783 3507 Fragments arranged by size: 3507 1783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4214 4388 65 Size: 1183 2966 174 1396 Fragments arranged by size: 2966 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2538 2835 2891 4363 4550 1700 Size: 110 728 297 56 1472 187 2869 Fragments arranged by size: 2869 1472 728 297 187 110 56 BsrGI T'GTAC_A Cuts at: 96 2523 96 Size: 2427 3292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 3779 4791 3779 Size: 1012 4707 BstAPI GCAn_nnn'nTGC Cuts at: 794 2515 794 Size: 1721 3998 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5719 BstYI r'GATC_y Cuts at: 1972 2172 2239 3049 3416 3801 3818 5270 5356 Size: 200 67 810 367 385 17 1452 86 Cuts at: 5356 5368 5714 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 810 385 367 346 200 86 67 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2748 513 Size: 552 939 744 3484 Fragments arranged by size: 3484 939 744 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2177 487 Size: 683 56 951 4029 Fragments arranged by size: 4029 951 683 56 BtsI GCAGTG_nn' Cuts at: 3235 4029 4049 3235 Size: 794 20 4905 Fragments arranged by size: 4905 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 2400 3023 2400 Size: 623 5096 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2460 2733 Size: 54 1425 50 275 18 336 294 273 Cuts at: 2733 2751 2820 2833 2850 2889 3166 4055 5684 Size: 18 69 13 17 39 277 889 1629 Cuts at: 5684 8 Size: 43 Fragments arranged by size: 1629 1425 889 336 294 277 275 273 69 54 50 43 39 18 18 17 13 EagI C'GGCC_G Cuts at: 1537 1812 2751 2833 3166 1537 Size: 275 939 82 333 4090 Fragments arranged by size: 4090 939 333 275 82 EarI CTCTTCn'nnn_ Cuts at: 1353 3649 1353 Size: 2296 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4306 4680 4826 387 Size: 1064 138 2717 374 146 1280 Fragments arranged by size: 2717 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3011 3011 Size: 5719 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2326 3782 4379 5166 5434 1239 Size: 393 566 128 1456 597 787 268 1524 Fragments arranged by size: 1524 1456 787 597 566 393 268 128 EcoO109I rG'GnC_Cy Cuts at: 2310 2589 2739 2740 2310 Size: 279 150 1 5289 Fragments arranged by size: 5289 279 150 1 EcoRI G'AATT_C Cuts at: 3028 3406 3028 Size: 378 5341 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2530 2530 Size: 5719 FspI TGC'GCA Cuts at: 2015 4225 2015 Size: 2210 3509 HaeII r_GCGC'y Cuts at: 1678 2133 2515 2995 4866 1678 Size: 455 382 480 1871 2531 Fragments arranged by size: 2531 1871 480 455 382 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2218 2224 2250 2256 4360 Size: 32 135 32 646 6 26 6 2104 Cuts at: 4360 4392 4434 4466 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2104 646 135 42 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2326 2683 3063 3309 669 Size: 988 281 388 357 380 246 3079 Fragments arranged by size: 3079 988 388 380 357 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5719 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2082 4833 5017 1305 Size: 777 2751 184 2007 Fragments arranged by size: 2751 2007 777 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2462 2822 2852 10 Size: 54 1425 343 630 360 30 2877 Fragments arranged by size: 2877 1425 630 360 343 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2336 3677 4036 4195 518 Size: 648 981 189 1341 359 159 2042 Fragments arranged by size: 2042 1341 981 648 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2019 2553 2617 2750 2832 2998 3135 3812 Size: 413 534 64 133 82 166 137 677 Cuts at: 3812 4960 5205 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 534 413 245 166 137 133 82 64 NaeI GCC'GGC Cuts at: 2837 2893 2837 Size: 56 5663 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4228 Fragments arranged by size: 4228 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NgoMIV G'CCGG_C Cuts at: 2835 2891 2835 Size: 56 5663 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 2751 3166 2751 Size: 415 5304 NspI r_CATG'y Cuts at: 1132 1229 3077 4622 1132 Size: 97 1848 1545 2229 Fragments arranged by size: 2229 1848 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5719 PciI A'CATG_T Cuts at: 4618 4618 Size: 5719 PflMI CCAn_nnn'nTGG Cuts at: 1108 2041 2952 1108 Size: 933 911 3875 Fragments arranged by size: 3875 933 911 PfoI T'CCnGG_A Cuts at: 2175 2175 Size: 5719 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PspOMI G'GGCC_C Cuts at: 2739 2739 Size: 5719 PstI C_TGCA'G Cuts at: 830 2673 3071 830 Size: 1843 398 3478 Fragments arranged by size: 3478 1843 398 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 PvuII CAG'CTG Cuts at: 2553 2617 2998 2553 Size: 64 381 5274 Fragments arranged by size: 5274 381 64 RsrII CG'GwC_CG Cuts at: 2292 2292 Size: 5719 SacI G_AGCT'C Cuts at: 3013 3013 Size: 5719 SacII CC_GC'GG Cuts at: 2751 2751 Size: 5719 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SbfI CC_TGCA'GG Cuts at: 2673 3071 2673 Size: 398 5321 ScaI AGT'ACT Cuts at: 2978 3967 5679 2978 Size: 989 1712 3018 Fragments arranged by size: 3018 1712 989 SfcI C'TryA_G Cuts at: 826 1045 2669 3067 4202 4883 5074 826 Size: 219 1624 398 1135 681 191 1471 Fragments arranged by size: 1624 1471 1135 681 398 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 3046 1872 Size: 1174 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 3823 4724 4986 820 Size: 197 926 333 1547 901 262 1553 Fragments arranged by size: 1553 1547 926 901 333 262 197 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 3077 1132 Size: 1945 3774 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5719 StyI C'CwwG_G Cuts at: 513 1065 2004 2419 3158 513 Size: 552 939 415 739 3074 Fragments arranged by size: 3074 939 739 552 415 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 2176 2309 2330 2591 3746 3763 Size: 1255 579 17 133 21 261 1155 17 Cuts at: 3763 3916 4101 4546 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1155 579 445 261 185 153 133 21 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 2523 2976 Size: 274 80 33 51 1443 276 270 453 Cuts at: 2976 3965 5677 96 Size: 989 1712 138 Fragments arranged by size: 1712 1443 989 453 276 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5679 5711 5679 Size: 32 5687 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2764 2995 2764 Size: 231 5488 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5719 XmaI C'CCGG_G Cuts at: 1870 3044 1870 Size: 1174 4545 XmnI GAAnn'nnTTC Cuts at: 3848 3848 Size: 5719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2773 3527 276 Size: 53 83 186 936 1239 754 2468 Fragments arranged by size: 2468 1239 936 754 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI SanDI SapI SexAI SfiI SfoI SrfI SwaI