Restriction map for
pFN21AE2398
(Linear) (Six-base) MAPSORT of: KIEE2398.seq Check: 3429 from: 1 to: 1014
KIEE2398.orf
With 182 enzymes: SgfI *
February 7, 2012 15:11 ..
AatII G_ACGT'C
Cuts at: 0 770 1014
Size: 770 244
AfeI AGC'GCT
Cuts at: 0 508 1014
Size: 508 506
AgeI A'CCGG_T
Cuts at: 0 533 1014
Size: 533 481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 564 1014
Size: 564 450
ApaI G_GGCC'C
Cuts at: 0 738 1014
Size: 738 276
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 240 273 1014
Size: 240 33 741
Fragments arranged by size:
741 240 33
BamHI G'GATC_C
Cuts at: 0 167 1014
Size: 167 847
BanII G_rGCy'C
Cuts at: 0 270 738 1008 1014
Size: 270 468 270 6
Fragments arranged by size:
468 270 270 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 214 1014
Size: 214 800
BclI T'GATC_A
Cuts at: 0 329 1014
Size: 329 685
BglI GCCn_nnn'nGGC
Cuts at: 0 68 887 1014
Size: 68 819 127
Fragments arranged by size:
819 127 68
Bme1580I G_kGCm'C
Cuts at: 0 738 1014
Size: 738 276
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 1014
Size: 240 774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 321 1014
Size: 193 128 693
Fragments arranged by size:
693 193 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1014
Size: 256 758
BsaHI Gr'CG_yC
Cuts at: 0 86 317 767 1014
Size: 86 231 450 247
Fragments arranged by size:
450 247 231 86
BsaWI w'CCGG_w
Cuts at: 0 533 554 593 686 857 1014
Size: 533 21 39 93 171 157
Fragments arranged by size:
533 171 157 93 39 21
BseYI C'CCAG_C
Cuts at: 0 445 1014
Size: 445 569
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 1014
Size: 359 655
BsiEI CG_ry'CG
Cuts at: 0 749 831 1014
Size: 749 82 183
Fragments arranged by size:
749 183 82
BsiHKAI G_wGCw'C
Cuts at: 0 179 1008 1014
Size: 179 829 6
Fragments arranged by size:
829 179 6
BsmI GAATG_Cn'
Cuts at: 0 221 1014
Size: 221 793
Bsp1286I G_dGCh'C
Cuts at: 0 179 270 738 1008 1014
Size: 179 91 468 270 6
Fragments arranged by size:
468 270 179 91 6
BspEI T'CCGG_A
Cuts at: 0 554 857 1014
Size: 554 303 157
Fragments arranged by size:
554 303 157
BspHI T'CATG_A
Cuts at: 0 326 1014
Size: 326 688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 663 1014
Size: 122 541 351
Fragments arranged by size:
541 351 122
BsrFI r'CCGG_y
Cuts at: 0 533 830 886 1014
Size: 533 297 56 128
Fragments arranged by size:
533 297 128 56
BsrGI T'GTAC_A
Cuts at: 0 518 1014
Size: 518 496
BstAPI GCAn_nnn'nTGC
Cuts at: 0 510 1014
Size: 510 504
BstYI r'GATC_y
Cuts at: 0 167 234 1014
Size: 167 67 780
Fragments arranged by size:
780 167 67
BtgI C'CryG_G
Cuts at: 0 743 1014
Size: 743 271
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 172 1014
Size: 172 842
DraI TTT'AAA
Cuts at: 0 395 1014
Size: 395 619
EaeI y'GGCC_r
Cuts at: 0 161 455 728 746 815 828 845 884
Size: 161 294 273 18 69 13 17 39
Cuts at: 884 1014
Size: 130
Fragments arranged by size:
294 273 161 130 69 39 18 17
13
EagI C'GGCC_G
Cuts at: 0 746 828 1014
Size: 746 82 186
Fragments arranged by size:
746 186 82
EcoICRI GAG'CTC
Cuts at: 0 1006 1014
Size: 1006 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 321 1014
Size: 193 128 693
Fragments arranged by size:
693 193 128
EcoO109I rG'GnC_Cy
Cuts at: 0 305 584 734 735 1014
Size: 305 279 150 1 279
Fragments arranged by size:
305 279 279 150 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 525 1014
Size: 525 489
FspI TGC'GCA
Cuts at: 0 10 1014
Size: 10 1004
HaeII r_GCGC'y
Cuts at: 0 128 510 990 1014
Size: 128 382 480 24
Fragments arranged by size:
480 382 128 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 213 219 245 251 1014
Size: 213 6 26 6 763
Fragments arranged by size:
763 213 26 6 6
HincII GTy'rAC
Cuts at: 0 321 678 1014
Size: 321 357 336
Fragments arranged by size:
357 336 321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 1014
Size: 77 937
MscI TGG'CCA
Cuts at: 0 457 817 847 1014
Size: 457 360 30 167
Fragments arranged by size:
457 360 167 30
MslI CAynn'nnrTG
Cuts at: 0 142 331 1014
Size: 142 189 683
Fragments arranged by size:
683 189 142
MspA1I CmG'CkG
Cuts at: 0 14 548 612 745 827 993 1014
Size: 14 534 64 133 82 166 21
Fragments arranged by size:
534 166 133 82 64 21 14
NaeI GCC'GGC
Cuts at: 0 832 888 1014
Size: 832 56 126
Fragments arranged by size:
832 126 56
NgoMIV G'CCGG_C
Cuts at: 0 830 886 1014
Size: 830 56 128
Fragments arranged by size:
830 128 56
NotI GC'GGCC_GC
Cuts at: 0 746 1014
Size: 746 268
PflMI CCAn_nnn'nTGG
Cuts at: 0 36 947 1014
Size: 36 911 67
Fragments arranged by size:
911 67 36
PfoI T'CCnGG_A
Cuts at: 0 170 1014
Size: 170 844
PspOMI G'GGCC_C
Cuts at: 0 734 1014
Size: 734 280
PstI C_TGCA'G
Cuts at: 0 668 1014
Size: 668 346
PvuII CAG'CTG
Cuts at: 0 548 612 993 1014
Size: 548 64 381 21
Fragments arranged by size:
548 381 64 21
RsrII CG'GwC_CG
Cuts at: 0 287 1014
Size: 287 727
SacI G_AGCT'C
Cuts at: 0 1008 1014
Size: 1008 6
SacII CC_GC'GG
Cuts at: 0 746 1014
Size: 746 268
SbfI CC_TGCA'GG
Cuts at: 0 668 1014
Size: 668 346
ScaI AGT'ACT
Cuts at: 0 973 1014
Size: 973 41
SfcI C'TryA_G
Cuts at: 0 664 1014
Size: 664 350
SmlI C'TyrA_G
Cuts at: 0 271 1014
Size: 271 743
StyI C'CwwG_G
Cuts at: 0 414 1014
Size: 414 600
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 154 171 304 325 586 1014
Size: 154 17 133 21 261 428
Fragments arranged by size:
428 261 154 133 21 17
TatI w'GTAC_w
Cuts at: 0 248 518 971 1014
Size: 248 270 453 43
Fragments arranged by size:
453 270 248 43
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 759 990 1014
Size: 759 231 24
Fragments arranged by size:
759 231 24
ZraI GAC'GTC
Cuts at: 0 768 1014
Size: 768 246
Enzymes that do cut:
AatII AfeI AgeI AlfI ApaI BaeI BamHI BanII
BbsI BclI BglI Bme1580I BmrI BpmI BpuEI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrFI BsrGI BstAPI BstYI BtgI BtgZI
DraI EaeI EagI EcoICRI Eco57MI EcoO109I FalI FspI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NgoMIV NotI PflMI PfoI PspOMI PstI PvuII RsrII
SacI SacII SbfI ScaI SfcI SmlI StyI TaqII
TatI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BanI BbeI BbvCI BcgI BciVI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaXI BseRI BsiWI BsmBI BsrBI BsrDI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI
CspCI DraIII DrdI EarI EciI EcoNI EcoRI EcoRV
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI SalI SanDI SapI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI