################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AciI 969 GCGG CCGC(-3/-1) AcsI 360 AAATTT [GA]^AATT[CT] AflIII 851 ACATGT A^C[GA][CT]GT ApoI 360 AAATTT [GA]^AATT[CT] Asp700I 184 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 743 GGTCC G^G[AT]CC BamHI 221 GGATCC G^GATCC BbsI 316 GAAGAC GAAGAC(2/6) BciVI 241 GTATCC GTATCC(6/5) BclI 662 TGATCA T^GATCA BfaI 4 CTAG C^TAG BpmI 20 CTCCAG CTGGAG(16/14) BpuAI 316 GAAGAC GAAGAC(2/6) BsgI 537 CTGCAC GTGCAG(16/14) BsmAI 708 GAGAC GTCTC(1/5) BsmI 334 GCATTC GAATGC(1/-1) BspHI 266 TCATGA T^CATGA BspLU11I 851 ACATGT A^CATGT BsrI 463 ACTGG ACTGG(1/-1) BstAPI 518 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 672 CATCC GGATG(2/0) BstXI 997 CCAAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 221 GGATCC [GA]^GATC[CT] Cac8I 913 GCCAGC GC[ACGT]^[ACGT]GC EarI 956 GAAGAG CTCTTC(1/4) EcoT22I 332 ATGCAT ATGCA^T FbaI 662 TGATCA T^GATCA FokI 672 CATCC GGATG(9/13) HaeIII 777 GGCC GG^CC HgaI 319 GACGC GACGC(5/10) HphI 101 TCACC GGTGA(8/7) Ksp632I 956 GAAGAG CTCTTC(1/4) MaeI 4 CTAG C^TAG MflI 221 GGATCC [GA]^GATC[CT] MspA1I 179 CAGCTG C[AC]G^C[GT]G NsiI 332 ATGCAT ATGCA^T PstI 71 CTGCAG CTGCA^G PvuII 179 CAGCTG CAG^CTG RcaI 266 TCATGA T^CATGA SapI 956 GAAGAGC GCTCTTC(1/4) SfaNI 527 GCATC GCATC(5/9) SfcI 71 CTGCAG C^T[GA][CT]AG SmlI 703 CTCAAG C^T[CT][GA]AG TaqI 981 TCGA T^CGA TfiI 933 GATTC G^A[AT]TC TspRI 540 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 981 TCGA T^CGA XhoII 221 GGATCC [GA]^GATC[CT] XmnI 184 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### BseRI 573 CTCCTC GAGGAG(10/8) 656 CTCCTC BsrDI 147 CATTGC GCAATG(2/0) 731 GCAATG Cfr13I 743 GGTCC G^G[ACGT]CC 776 GGGCC PleI 654 GACTC GAGTC(4/5) 1008 GACTC Sau96I 743 GGTCC G^G[ACGT]CC 776 GGGCC Tsp45I 512 GTCAC ^GT[GC]AC 652 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AluI AlwI BbvI BsaJI BsiYI BslI BssKI BstNI DdeI DpnII EcoRII Fnu4HI HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI Sau3AI ScrFI Tru9I TseI Tsp509I