(Circular) (Six-base) MAPSORT of: pFN21AE5485.seq Check: 4897 from: 1 to: 5719 pFN21AE5485 5719 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3529 278 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3062 1937 Size: 1125 4594 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 2497 2963 3782 5166 5434 1632 Size: 708 157 466 819 1384 268 1917 Fragments arranged by size: 1917 1384 819 708 466 268 157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 2856 4618 5595 1203 Size: 1653 1762 977 1327 Fragments arranged by size: 1762 1653 1327 977 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 4448 4448 Size: 5719 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5719 AlwNI CAG_nnn'CTG Cuts at: 3462 5034 3462 Size: 1572 4147 ApaLI G'TGCA_C Cuts at: 3776 4932 3776 Size: 1156 4563 ApoI r'AATT_y Cuts at: 1493 2365 3028 3254 3406 5515 1493 Size: 872 663 226 152 2109 1697 Fragments arranged by size: 2109 1697 872 663 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 3044 1870 Size: 73 1101 4545 Fragments arranged by size: 4545 1101 73 BamHI G'GATC_C Cuts at: 2226 3049 3416 2226 Size: 823 367 4529 Fragments arranged by size: 4529 823 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3040 4495 618 Size: 325 217 627 1253 1455 1842 Fragments arranged by size: 1842 1455 1253 627 325 217 BanII G_rGCy'C Cuts at: 1391 3038 1391 Size: 1647 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2328 928 Size: 971 429 4319 Fragments arranged by size: 4319 971 429 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3910 3944 1903 Size: 34 50 34 1889 34 3678 Fragments arranged by size: 3678 1889 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2257 3611 4821 2257 Size: 1354 1210 3155 Fragments arranged by size: 3155 1354 1210 BclI T'GATC_A Cuts at: 1548 2667 1548 Size: 1119 4600 BfrBI ATG'CAT Cuts at: 2339 2339 Size: 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4330 136 Size: 107 122 71 631 605 2658 1525 Fragments arranged by size: 2658 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5714 5714 Size: 5719 BlpI GC'TnA_GC Cuts at: 3136 3136 Size: 5719 Bme1580I G_kGCm'C Cuts at: 1790 3780 4936 1790 Size: 1990 1156 2573 Fragments arranged by size: 2573 1990 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5719 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3450 4408 442 Size: 416 53 83 399 24 2033 958 1753 Fragments arranged by size: 2033 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2010 4379 1239 Size: 771 2369 2579 Fragments arranged by size: 2579 2369 771 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2693 3844 4709 5007 2693 Size: 1151 865 298 3405 Fragments arranged by size: 3405 1151 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4382 882 Size: 617 2883 2219 Fragments arranged by size: 2883 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3510 493 Size: 711 2306 2702 Fragments arranged by size: 2702 2306 711 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3526 3908 4543 275 Size: 53 83 186 936 1993 382 635 1451 Fragments arranged by size: 1993 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4152 4550 4824 4971 5382 4152 Size: 398 274 147 411 4489 Fragments arranged by size: 4489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5494 Fragments arranged by size: 5494 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2569 2652 1186 Size: 1383 83 4253 Fragments arranged by size: 4253 1383 83 BseYI C'CCAG_C Cuts at: 2759 4922 5418 2759 Size: 2163 496 3060 Fragments arranged by size: 3060 2163 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2527 1163 Size: 573 791 4355 Fragments arranged by size: 4355 791 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3169 3930 4958 1540 Size: 275 186 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3780 3865 4936 3780 Size: 85 1071 4563 Fragments arranged by size: 4563 1071 85 BsmI GAATG_Cn' Cuts at: 2339 3230 3323 2339 Size: 891 93 4735 Fragments arranged by size: 4735 891 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3038 3780 3865 4936 1391 Size: 399 1248 742 85 1071 2174 Fragments arranged by size: 2174 1248 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5382 5382 Size: 5719 BspHI T'CATG_A Cuts at: 2271 3608 5338 2271 Size: 1337 1730 2652 Fragments arranged by size: 2652 1730 1337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3074 844 Size: 2230 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3606 1394 Size: 429 1783 3507 Fragments arranged by size: 3507 1783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2151 2743 4214 4388 65 Size: 1183 903 592 1471 174 1396 Fragments arranged by size: 1471 1396 1183 903 592 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4363 4550 1700 Size: 110 2553 187 2869 Fragments arranged by size: 2869 2553 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 3779 4791 3779 Size: 1012 4707 BstAPI GCAn_nnn'nTGC Cuts at: 794 2529 794 Size: 1735 3984 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5719 BstXI CCAn_nnnn'nTGG Cuts at: 3009 3009 Size: 5719 BstYI r'GATC_y Cuts at: 1972 2226 3049 3416 3801 3818 5270 5356 5368 Size: 254 823 367 385 17 1452 86 12 Cuts at: 5368 5714 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 823 385 367 346 254 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5167 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4980 Fragments arranged by size: 4980 683 56 BtsI GCAGTG_nn' Cuts at: 3235 4029 4049 3235 Size: 794 20 4905 Fragments arranged by size: 4905 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3166 4055 5684 Size: 54 1425 50 275 18 1336 889 1629 Cuts at: 5684 8 Size: 43 Fragments arranged by size: 1629 1425 1336 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3166 1537 Size: 275 1354 4090 Fragments arranged by size: 4090 1354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2956 3649 1353 Size: 1603 693 3423 Fragments arranged by size: 3423 1603 693 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4306 4680 4826 387 Size: 1064 138 2717 374 146 1280 Fragments arranged by size: 2717 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2010 2340 2497 2963 3782 4379 5166 Size: 393 378 330 157 466 819 597 787 Cuts at: 5166 5434 1239 Size: 268 1524 Fragments arranged by size: 1524 819 787 597 466 393 378 330 268 157 EcoRI G'AATT_C Cuts at: 3028 3406 3028 Size: 378 5341 FspI TGC'GCA Cuts at: 4225 4225 Size: 5719 HaeII r_GCGC'y Cuts at: 1678 4866 1678 Size: 3188 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2644 2676 2689 2721 4360 Size: 32 135 32 1072 32 13 32 1639 Cuts at: 4360 4392 4434 4466 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1639 1072 135 42 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 3063 3309 669 Size: 988 281 1125 246 3079 Fragments arranged by size: 3079 1125 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5719 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 2775 2877 4833 5017 1305 Size: 1302 168 102 1956 184 2007 Fragments arranged by size: 2007 1956 1302 184 168 102 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3897 Fragments arranged by size: 3897 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3677 4036 4195 518 Size: 648 2511 359 159 2042 Fragments arranged by size: 2511 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2186 3135 3812 4960 5205 1606 Size: 580 949 677 1148 245 2120 Fragments arranged by size: 2120 1148 949 677 580 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5167 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NsiI A_TGCA'T Cuts at: 2341 2341 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 2183 2527 2860 3077 4622 1132 Size: 97 954 344 333 217 1545 2229 Fragments arranged by size: 2229 1545 954 344 333 217 97 PasI CC'CwG_GG Cuts at: 1348 2951 1348 Size: 1603 4116 PciI A'CATG_T Cuts at: 2856 4618 2856 Size: 1762 3957 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5719 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PstI C_TGCA'G Cuts at: 830 2080 3071 830 Size: 1250 991 3478 Fragments arranged by size: 3478 1250 991 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 PvuII CAG'CTG Cuts at: 2186 2186 Size: 5719 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SapI GCTCTTCn'nnn_ Cuts at: 2956 2956 Size: 5719 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 3967 5679 3967 Size: 1712 4007 SfcI C'TryA_G Cuts at: 826 1045 2076 3067 4202 4883 5074 826 Size: 219 1031 991 1135 681 191 1471 Fragments arranged by size: 1471 1135 1031 991 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 3046 1872 Size: 1174 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2708 3823 4724 4986 820 Size: 197 926 765 1115 901 262 1553 Fragments arranged by size: 1553 1115 926 901 765 262 197 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 3077 1132 Size: 1945 3774 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5719 StyI C'CwwG_G Cuts at: 513 1065 3158 513 Size: 552 2093 3074 Fragments arranged by size: 3074 2093 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 3746 3763 3916 4101 4546 325 Size: 1255 1035 1131 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1131 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3965 5677 96 Size: 274 80 33 51 1443 1988 1712 138 Fragments arranged by size: 1988 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5679 5711 5679 Size: 32 5687 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5719 XmaI C'CCGG_G Cuts at: 1870 3044 1870 Size: 1174 4545 XmnI GAAnn'nnTTC Cuts at: 2193 3848 2193 Size: 1655 4064 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3527 276 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI