(Circular) (Six-base) MAPSORT of: pFN21AE0800.seq Check: 2873 from: 1 to: 9298 pFN21AE0800 9298 bp With 182 enzymes: SgfI * March 28, 2012 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7108 278 Size: 53 83 186 936 5572 2468 Fragments arranged by size: 5572 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6641 1937 Size: 4704 4594 Acc65I G'GTAC_C Cuts at: 4376 6619 4376 Size: 2243 7055 AclI AA'CG_TT Cuts at: 1563 7425 7798 1563 Size: 5862 373 3063 Fragments arranged by size: 5862 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3536 3954 4584 4944 5649 5757 7361 8745 Size: 1904 418 630 360 705 108 1604 1384 Cuts at: 8745 9013 1632 Size: 268 1917 Fragments arranged by size: 1917 1904 1604 1384 705 630 418 360 268 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9101 AflIII A'CryG_T Cuts at: 1203 2648 2820 2977 3081 4476 8197 9174 1203 Size: 1445 172 157 104 1395 3721 977 1327 Fragments arranged by size: 3721 1445 1395 1327 977 172 157 104 AgeI A'CCGG_T Cuts at: 6293 8129 6293 Size: 1836 7462 AhdI GACnn_n'nnGTC Cuts at: 2484 8027 2484 Size: 5543 3755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9298 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9298 AlwNI CAG_nnn'CTG Cuts at: 4259 5366 7041 8613 4259 Size: 1107 1675 1572 4944 Fragments arranged by size: 4944 1675 1572 1107 ApaLI G'TGCA_C Cuts at: 7355 8511 7355 Size: 1156 8142 ApoI r'AATT_y Cuts at: 1493 2531 3194 3315 3719 3785 4126 4324 4904 Size: 1038 663 121 404 66 341 198 580 Cuts at: 4904 5978 6346 6607 6833 6985 9094 1493 Size: 1074 368 261 226 152 2109 1697 Fragments arranged by size: 2109 1697 1074 1038 663 580 404 368 341 261 226 198 152 121 66 AseI AT'TA_AT Cuts at: 160 1032 3666 5222 7852 160 Size: 872 2634 1556 2630 1606 Fragments arranged by size: 2634 2630 1606 1556 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9298 AvaI C'yCGr_G Cuts at: 1870 1943 6528 6623 1870 Size: 73 4585 95 4545 Fragments arranged by size: 4585 4545 95 73 AvrII C'CTAG_G Cuts at: 5116 5116 Size: 9298 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4884 4917 4884 Size: 33 9265 BamHI G'GATC_C Cuts at: 2747 5257 6334 6628 6995 2747 Size: 2510 1077 294 367 5050 Fragments arranged by size: 5050 2510 1077 367 294 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3854 4376 4841 6296 6619 Size: 325 217 627 2067 522 465 1455 323 Cuts at: 6619 8074 618 Size: 1455 1842 Fragments arranged by size: 2067 1842 1455 1455 627 522 465 325 323 217 BanII G_rGCy'C Cuts at: 1391 6617 1391 Size: 5226 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3542 3596 4027 4260 4856 4960 5517 Size: 971 1643 54 431 233 596 104 557 Cuts at: 5517 6168 6263 6270 928 Size: 651 95 7 3956 Fragments arranged by size: 3956 1643 971 651 596 557 431 233 104 95 54 7 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7489 7523 1903 Size: 34 5552 34 3678 Fragments arranged by size: 5552 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3920 7190 8400 3920 Size: 3270 1210 4818 Fragments arranged by size: 4818 3270 1210 BclI T'GATC_A Cuts at: 1548 2995 4471 1548 Size: 1447 1476 6375 Fragments arranged by size: 6375 1476 1447 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7909 136 Size: 107 122 71 631 605 6237 1525 Fragments arranged by size: 6237 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4142 9293 4142 Size: 5151 4147 BlpI GC'TnA_GC Cuts at: 4433 6715 4433 Size: 2282 7016 Bme1580I G_kGCm'C Cuts at: 1790 3857 7359 8515 1790 Size: 2067 3502 1156 2573 Fragments arranged by size: 3502 2573 2067 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3253 4587 6221 Size: 416 53 83 399 24 1836 1334 1634 Cuts at: 6221 7029 7987 442 Size: 808 958 1753 Fragments arranged by size: 1836 1753 1634 1334 958 808 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9298 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3825 3825 Size: 9298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3734 7958 1239 Size: 2495 4224 2579 Fragments arranged by size: 4224 2579 2495 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2606 5733 7423 8288 8586 2606 Size: 3127 1690 865 298 3318 Fragments arranged by size: 3318 3127 1690 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7961 882 Size: 617 6462 2219 Fragments arranged by size: 6462 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5020 7089 493 Size: 711 3816 2069 2702 Fragments arranged by size: 3816 2702 2069 711 BsaBI GATnn'nnATC Cuts at: 5256 9292 5256 Size: 4036 5262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7105 7487 8122 275 Size: 53 83 186 936 5572 382 635 1451 Fragments arranged by size: 5572 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2335 6293 7731 8129 8403 8550 8961 2335 Size: 3958 1438 398 274 147 411 2672 Fragments arranged by size: 3958 2672 1438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 9073 Fragments arranged by size: 9073 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4848 6516 1186 Size: 3662 1668 3968 Fragments arranged by size: 3968 3662 1668 BseYI C'CCAG_C Cuts at: 2178 2416 5283 8501 8997 2178 Size: 238 2867 3218 496 2479 Fragments arranged by size: 3218 2867 2479 496 238 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 8725 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6748 7509 8537 1540 Size: 275 186 4747 761 1028 2301 Fragments arranged by size: 4747 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 7359 7444 8515 7359 Size: 85 1071 8142 Fragments arranged by size: 8142 1071 85 BsmI GAATG_Cn' Cuts at: 5127 6809 6902 5127 Size: 1682 93 7523 Fragments arranged by size: 7523 1682 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3857 6617 7359 7444 8515 1391 Size: 399 2067 2760 742 85 1071 2174 Fragments arranged by size: 2760 2174 2067 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2335 8961 2335 Size: 6626 2672 BspHI T'CATG_A Cuts at: 7187 8917 7187 Size: 1730 7568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6653 844 Size: 5809 3489 BsrBI CCG'CTC Cuts at: 1394 1823 7185 1394 Size: 429 5362 3507 Fragments arranged by size: 5362 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2018 2952 7793 7967 65 Size: 1183 770 934 4841 174 1396 Fragments arranged by size: 4841 1396 1183 934 770 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6293 7942 8129 1700 Size: 110 4483 1649 187 2869 Fragments arranged by size: 4483 2869 1649 187 110 BsrGI T'GTAC_A Cuts at: 96 2247 96 Size: 2151 7147 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9298 BssSI C'ACGA_G Cuts at: 5940 7358 8370 5940 Size: 1418 1012 6868 Fragments arranged by size: 6868 1418 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9298 BstBI TT'CG_AA Cuts at: 6452 8102 6452 Size: 1650 7648 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9298 BstXI CCAn_nnnn'nTGG Cuts at: 3406 4247 3406 Size: 841 8457 BstYI r'GATC_y Cuts at: 1972 2747 4142 5257 6334 6628 6995 7380 7397 Size: 775 1395 1115 1077 294 367 385 17 Cuts at: 7397 8849 8935 8947 9293 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1395 1115 1077 775 385 367 346 294 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4047 4047 Size: 9298 BtgI C'CryG_G Cuts at: 513 1065 2004 4542 5954 513 Size: 552 939 2538 1412 3857 Fragments arranged by size: 3857 2538 1412 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8559 Fragments arranged by size: 8559 683 56 BtsI GCAGTG_nn' Cuts at: 5917 6169 6814 7608 7628 5917 Size: 252 645 794 20 7587 Fragments arranged by size: 7587 794 645 252 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9298 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9263 DraI TTT'AAA Cuts at: 2683 5031 6602 2683 Size: 2348 1571 5379 Fragments arranged by size: 5379 2348 1571 DraIII CAC_nnn'GTG Cuts at: 3366 3366 Size: 9298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8305 809 Size: 507 6989 1802 Fragments arranged by size: 6989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6745 7634 9263 Size: 54 1425 50 275 18 4915 889 1629 Cuts at: 9263 8 Size: 43 Fragments arranged by size: 4915 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6745 1537 Size: 275 4933 4090 Fragments arranged by size: 4933 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2596 2722 3699 3786 3794 5310 5867 6567 Size: 1243 126 977 87 8 1516 557 700 Cuts at: 6567 7228 1353 Size: 661 3423 Fragments arranged by size: 3423 1516 1243 977 700 661 557 126 87 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2129 7885 8259 8405 387 Size: 1064 138 540 5756 374 146 1280 Fragments arranged by size: 5756 1280 1064 540 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3536 3734 3954 4584 4944 5649 5757 Size: 393 1904 198 220 630 360 705 108 Cuts at: 5757 7361 7958 8745 9013 1239 Size: 1604 597 787 268 1524 Fragments arranged by size: 1904 1604 1524 787 705 630 597 393 360 268 220 198 108 EcoNI CCTnn'n_nnAGG Cuts at: 3383 3462 5589 3383 Size: 79 2127 7092 Fragments arranged by size: 7092 2127 79 EcoO109I rG'GnC_Cy Cuts at: 2299 2299 Size: 9298 EcoRI G'AATT_C Cuts at: 3315 6346 6607 6985 3315 Size: 3031 261 378 5628 Fragments arranged by size: 5628 3031 378 261 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2789 5597 6294 2789 Size: 2808 697 5793 Fragments arranged by size: 5793 2808 697 FspI TGC'GCA Cuts at: 7804 7804 Size: 9298 HaeII r_GCGC'y Cuts at: 1678 8445 1678 Size: 6767 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2470 2502 3532 3564 3894 Size: 32 135 32 898 32 1030 32 330 Cuts at: 3894 3926 3967 3999 4843 4875 5882 5914 6413 Size: 32 41 32 844 32 1007 32 499 Cuts at: 6413 6445 6497 6529 7939 7971 8013 8045 1373 Size: 32 52 32 1410 32 42 32 2626 Fragments arranged by size: 2626 1410 1030 1007 898 844 499 330 135 52 42 41 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2050 2947 3999 6642 6888 669 Size: 988 281 112 897 1052 2643 246 3079 Fragments arranged by size: 3079 2643 1052 988 897 281 246 112 HindIII A'AGCT_T Cuts at: 748 3489 748 Size: 2741 6557 HpaI GTT'AAC Cuts at: 3999 6888 3999 Size: 2889 6409 KpnI G_GTAC'C Cuts at: 4380 6623 4380 Size: 2243 7055 MfeI C'AATT_G Cuts at: 2120 2328 3182 6897 2120 Size: 208 854 3715 4521 Fragments arranged by size: 4521 3715 854 208 MluI A'CGCG_T Cuts at: 9174 9174 Size: 9298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2820 4933 5031 5557 8412 8596 1305 Size: 1515 2113 98 526 2855 184 2007 Fragments arranged by size: 2855 2113 2007 1515 526 184 98 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7476 Fragments arranged by size: 7476 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4281 5681 7256 7615 7774 518 Size: 648 843 2272 1400 1575 359 159 2042 Fragments arranged by size: 2272 2042 1575 1400 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3290 6518 6714 7391 8539 8784 1606 Size: 1684 3228 196 677 1148 245 2120 Fragments arranged by size: 3228 2120 1684 1148 677 245 196 NcoI C'CATG_G Cuts at: 513 1065 2004 4542 513 Size: 552 939 2538 5269 Fragments arranged by size: 5269 2538 939 552 NdeI CA'TA_TG Cuts at: 387 2734 6501 387 Size: 2347 3767 3184 Fragments arranged by size: 3767 3184 2347 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9298 NotI GC'GGCC_GC Cuts at: 6745 6745 Size: 9298 NspI r_CATG'y Cuts at: 1132 1229 2652 2824 2981 3085 3180 4995 6656 Size: 97 1423 172 157 104 95 1815 1661 Cuts at: 6656 8201 1132 Size: 1545 2229 Fragments arranged by size: 2229 1815 1661 1545 1423 172 157 104 97 95 PacI TTA_AT'TAA Cuts at: 5226 5226 Size: 9298 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9298 PciI A'CATG_T Cuts at: 2648 2820 2977 3081 8197 2648 Size: 172 157 104 5116 3749 Fragments arranged by size: 5116 3749 172 157 104 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9298 PmeI GTTT'AAAC Cuts at: 6602 6602 Size: 9298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7367 7399 8880 8912 7367 Size: 32 1481 32 7753 Fragments arranged by size: 7753 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2299 2299 Size: 9298 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9298 PsiI TTA'TAA Cuts at: 6868 6868 Size: 9298 PstI C_TGCA'G Cuts at: 830 5441 6650 830 Size: 4611 1209 3478 Fragments arranged by size: 4611 3478 1209 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9298 PvuII CAG'CTG Cuts at: 3290 6518 3290 Size: 3228 6070 SalI G'TCGA_C Cuts at: 1936 6640 1936 Size: 4704 4594 SanDI GG'GwC_CC Cuts at: 2299 2299 Size: 9298 SapI GCTCTTCn'nnn_ Cuts at: 2596 2722 2596 Size: 126 9172 SbfI CC_TGCA'GG Cuts at: 6650 6650 Size: 9298 ScaI AGT'ACT Cuts at: 7546 9258 7546 Size: 1712 7586 SexAI A'CCwGG_T Cuts at: 4174 5812 4174 Size: 1638 7660 SfcI C'TryA_G Cuts at: 826 1045 2218 4873 5437 6646 7781 8462 8653 Size: 219 1173 2655 564 1209 1135 681 191 Cuts at: 8653 826 Size: 1471 Fragments arranged by size: 2655 1471 1209 1173 1135 681 564 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9298 SmaI CCC'GGG Cuts at: 1872 6625 1872 Size: 4753 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2621 5748 7402 8303 8565 820 Size: 197 926 678 3127 1654 901 262 1553 Fragments arranged by size: 3127 1654 1553 926 901 678 262 197 SnaBI TAC'GTA Cuts at: 493 7089 493 Size: 6596 2702 SpeI A'CTAG_T Cuts at: 152 4982 152 Size: 4830 4468 SphI G_CATG'C Cuts at: 1132 6656 1132 Size: 5524 3774 SspI AAT'ATT Cuts at: 5 52 3839 3923 5090 7222 5 Size: 47 3787 84 1167 2132 2081 Fragments arranged by size: 3787 2132 2081 1167 84 47 StuI AGG'CCT Cuts at: 1517 3420 5270 5775 1517 Size: 1903 1850 505 5040 Fragments arranged by size: 5040 1903 1850 505 StyI C'CwwG_G Cuts at: 513 1065 2004 2964 3421 4542 5116 5415 5553 Size: 552 939 960 457 1121 574 299 138 Cuts at: 5553 5604 6477 6737 513 Size: 51 873 260 3074 Fragments arranged by size: 3074 1121 960 939 873 574 552 457 299 260 138 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3235 4569 7325 7342 7495 7680 8125 Size: 1255 1655 1334 2756 17 153 185 445 Cuts at: 8125 325 Size: 1498 Fragments arranged by size: 2756 1655 1498 1334 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 3555 7544 Size: 274 80 33 51 1443 270 1308 3989 Cuts at: 7544 9256 96 Size: 1712 138 Fragments arranged by size: 3989 1712 1443 1308 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2221 2253 2317 2349 9258 9290 2221 Size: 32 64 32 6909 32 2229 Fragments arranged by size: 6909 2229 64 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9298 XbaI T'CTAG_A Cuts at: 4822 6634 4822 Size: 1812 7486 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2536 4312 5499 2536 Size: 1776 1187 6335 Fragments arranged by size: 6335 1776 1187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9298 XmaI C'CCGG_G Cuts at: 1870 6623 1870 Size: 4753 4545 XmnI GAAnn'nnTTC Cuts at: 2570 4818 7427 2570 Size: 2248 2609 4441 Fragments arranged by size: 4441 2609 2248 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7106 276 Size: 53 83 186 936 5572 2468 Fragments arranged by size: 5572 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI