Restriction map for
pFN21AE0800
(Linear) (Six-base) MAPSORT of: KIEE0800.seq Check: 5968 from: 1 to: 4593
KIEE0800.orf
With 182 enzymes: SgfI *
September 2, 2010 12:21 ..
Acc65I G'GTAC_C
Cuts at: 0 2371 4593
Size: 2371 2222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1531 1949 2579 2939 3644 3752 4593
Size: 1531 418 630 360 705 108 841
Fragments arranged by size:
1531 841 705 630 418 360 108
AflIII A'CryG_T
Cuts at: 0 643 815 972 1076 2471 4593
Size: 643 172 157 104 1395 2122
Fragments arranged by size:
2122 1395 643 172 157 104
AgeI A'CCGG_T
Cuts at: 0 4288 4593
Size: 4288 305
AhdI GACnn_n'nnGTC
Cuts at: 0 479 4593
Size: 479 4114
AlwNI CAG_nnn'CTG
Cuts at: 0 2254 3361 4593
Size: 2254 1107 1232
Fragments arranged by size:
2254 1232 1107
ApoI r'AATT_y
Cuts at: 0 526 1189 1310 1714 1780 2121 2319 2899
Size: 526 663 121 404 66 341 198 580
Cuts at: 2899 3973 4341 4593
Size: 1074 368 252
Fragments arranged by size:
1074 663 580 526 404 368 341 252
198 121 66
AseI AT'TA_AT
Cuts at: 0 1661 3217 4593
Size: 1661 1556 1376
Fragments arranged by size:
1661 1556 1376
AvaI C'yCGr_G
Cuts at: 0 4523 4593
Size: 4523 70
AvrII C'CTAG_G
Cuts at: 0 3111 4593
Size: 3111 1482
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2879 2912 4593
Size: 2879 33 1681
Fragments arranged by size:
2879 1681 33
BamHI G'GATC_C
Cuts at: 0 742 3252 4329 4593
Size: 742 2510 1077 264
Fragments arranged by size:
2510 1077 742 264
BanI G'GyrC_C
Cuts at: 0 1849 2371 2836 4291 4593
Size: 1849 522 465 1455 302
Fragments arranged by size:
1849 1455 522 465 302
BbsI GAAGACnn'nnnn_
Cuts at: 0 1537 1591 2022 2255 2851 2955 3512 4163
Size: 1537 54 431 233 596 104 557 651
Cuts at: 4163 4258 4265 4593
Size: 95 7 328
Fragments arranged by size:
1537 651 596 557 431 328 233 104
95 54 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 1915 4593
Size: 1915 2678
BclI T'GATC_A
Cuts at: 0 990 2466 4593
Size: 990 1476 2127
Fragments arranged by size:
2127 1476 990
BglII A'GATC_T
Cuts at: 0 2137 4593
Size: 2137 2456
BlpI GC'TnA_GC
Cuts at: 0 2428 4593
Size: 2428 2165
Bme1580I G_kGCm'C
Cuts at: 0 1852 4593
Size: 1852 2741
BmrI ACTGGGnnnn_n'
Cuts at: 0 1248 2582 4216 4593
Size: 1248 1334 1634 377
Fragments arranged by size:
1634 1334 1248 377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1820 4593
Size: 1820 2773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1729 4593
Size: 1729 2864
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 3728 4593
Size: 601 3127 865
Fragments arranged by size:
3127 865 601
BsaAI yAC'GTr
Cuts at: 0 3015 4593
Size: 3015 1578
BsaBI GATnn'nnATC
Cuts at: 0 3251 4593
Size: 3251 1342
BsaWI w'CCGG_w
Cuts at: 0 330 4288 4593
Size: 330 3958 305
Fragments arranged by size:
3958 330 305
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2843 4511 4593
Size: 2843 1668 82
Fragments arranged by size:
2843 1668 82
BseYI C'CCAG_C
Cuts at: 0 173 411 3278 4593
Size: 173 238 2867 1315
Fragments arranged by size:
2867 1315 238 173
BsmI GAATG_Cn'
Cuts at: 0 3122 4593
Size: 3122 1471
Bsp1286I G_dGCh'C
Cuts at: 0 1852 4593
Size: 1852 2741
BspEI T'CCGG_A
Cuts at: 0 330 4593
Size: 330 4263
BsrDI GCAATG_nn'
Cuts at: 0 13 947 4593
Size: 13 934 3646
Fragments arranged by size:
3646 934 13
BsrFI r'CCGG_y
Cuts at: 0 4288 4593
Size: 4288 305
BsrGI T'GTAC_A
Cuts at: 0 242 4593
Size: 242 4351
BssSI C'ACGA_G
Cuts at: 0 3935 4593
Size: 3935 658
BstBI TT'CG_AA
Cuts at: 0 4447 4593
Size: 4447 146
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1401 2242 4593
Size: 1401 841 2351
Fragments arranged by size:
2351 1401 841
BstYI r'GATC_y
Cuts at: 0 742 2137 3252 4329 4593
Size: 742 1395 1115 1077 264
Fragments arranged by size:
1395 1115 1077 742 264
Bsu36I CC'TnA_GG
Cuts at: 0 2042 4593
Size: 2042 2551
BtgI C'CryG_G
Cuts at: 0 2537 3949 4593
Size: 2537 1412 644
Fragments arranged by size:
2537 1412 644
BtsI GCAGTG_nn'
Cuts at: 0 3912 4164 4593
Size: 3912 252 429
Fragments arranged by size:
3912 429 252
DraI TTT'AAA
Cuts at: 0 678 3026 4593
Size: 678 2348 1567
Fragments arranged by size:
2348 1567 678
DraIII CAC_nnn'GTG
Cuts at: 0 1361 4593
Size: 1361 3232
EarI CTCTTCn'nnn_
Cuts at: 0 591 717 1694 1781 1789 3305 3862 4562
Size: 591 126 977 87 8 1516 557 700
Cuts at: 4562 4593
Size: 31
Fragments arranged by size:
1516 977 700 591 557 126 87 31
8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 124 4593
Size: 124 4469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1531 1729 1949 2579 2939 3644 3752 4593
Size: 1531 198 220 630 360 705 108 841
Fragments arranged by size:
1531 841 705 630 360 220 198 108
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1378 1457 3584 4593
Size: 1378 79 2127 1009
Fragments arranged by size:
2127 1378 1009 79
EcoO109I rG'GnC_Cy
Cuts at: 0 294 4593
Size: 294 4299
EcoRI G'AATT_C
Cuts at: 0 1310 4341 4593
Size: 1310 3031 252
Fragments arranged by size:
3031 1310 252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 784 3592 4289 4593
Size: 784 2808 697 304
Fragments arranged by size:
2808 784 697 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 465 497 1527 1559 1889 1921 1962 1994
Size: 465 32 1030 32 330 32 41 32
Cuts at: 1994 2838 2870 3877 3909 4408 4440 4492 4524
Size: 844 32 1007 32 499 32 52 32
Cuts at: 4524 4593
Size: 69
Fragments arranged by size:
1030 1007 844 499 465 330 69 52
41 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 45 942 1994 4593
Size: 45 897 1052 2599
Fragments arranged by size:
2599 1052 897 45
HindIII A'AGCT_T
Cuts at: 0 1484 4593
Size: 1484 3109
HpaI GTT'AAC
Cuts at: 0 1994 4593
Size: 1994 2599
KpnI G_GTAC'C
Cuts at: 0 2375 4593
Size: 2375 2218
MfeI C'AATT_G
Cuts at: 0 115 323 1177 4593
Size: 115 208 854 3416
Fragments arranged by size:
3416 854 208 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 2928 3026 3552 4593
Size: 815 2113 98 526 1041
Fragments arranged by size:
2113 1041 815 526 98
MslI CAynn'nnrTG
Cuts at: 0 2276 3676 4593
Size: 2276 1400 917
Fragments arranged by size:
2276 1400 917
MspA1I CmG'CkG
Cuts at: 0 1285 4513 4593
Size: 1285 3228 80
Fragments arranged by size:
3228 1285 80
NcoI C'CATG_G
Cuts at: 0 2537 4593
Size: 2537 2056
NdeI CA'TA_TG
Cuts at: 0 729 4496 4593
Size: 729 3767 97
Fragments arranged by size:
3767 729 97
NspI r_CATG'y
Cuts at: 0 647 819 976 1080 1175 2990 4593
Size: 647 172 157 104 95 1815 1603
Fragments arranged by size:
1815 1603 647 172 157 104 95
PacI TTA_AT'TAA
Cuts at: 0 3221 4593
Size: 3221 1372
PciI A'CATG_T
Cuts at: 0 643 815 972 1076 4593
Size: 643 172 157 104 3517
Fragments arranged by size:
3517 643 172 157 104
PpuMI rG'GwC_Cy
Cuts at: 0 294 4593
Size: 294 4299
PstI C_TGCA'G
Cuts at: 0 3436 4593
Size: 3436 1157
PvuII CAG'CTG
Cuts at: 0 1285 4513 4593
Size: 1285 3228 80
Fragments arranged by size:
3228 1285 80
SanDI GG'GwC_CC
Cuts at: 0 294 4593
Size: 294 4299
SapI GCTCTTCn'nnn_
Cuts at: 0 591 717 4593
Size: 591 126 3876
Fragments arranged by size:
3876 591 126
SexAI A'CCwGG_T
Cuts at: 0 2169 3807 4593
Size: 2169 1638 786
Fragments arranged by size:
2169 1638 786
SfcI C'TryA_G
Cuts at: 0 213 2868 3432 4593
Size: 213 2655 564 1161
Fragments arranged by size:
2655 1161 564 213
SmlI C'TyrA_G
Cuts at: 0 616 3743 4593
Size: 616 3127 850
Fragments arranged by size:
3127 850 616
SpeI A'CTAG_T
Cuts at: 0 2977 4593
Size: 2977 1616
SspI AAT'ATT
Cuts at: 0 1834 1918 3085 4593
Size: 1834 84 1167 1508
Fragments arranged by size:
1834 1508 1167 84
StuI AGG'CCT
Cuts at: 0 1415 3265 3770 4593
Size: 1415 1850 505 823
Fragments arranged by size:
1850 1415 823 505
StyI C'CwwG_G
Cuts at: 0 959 1416 2537 3111 3410 3548 3599 4472
Size: 959 457 1121 574 299 138 51 873
Cuts at: 4472 4593
Size: 121
Fragments arranged by size:
1121 959 873 574 457 299 138 121
51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1230 2564 4593
Size: 1230 1334 2029
Fragments arranged by size:
2029 1334 1230
TatI w'GTAC_w
Cuts at: 0 242 1550 4593
Size: 242 1308 3043
Fragments arranged by size:
3043 1308 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 216 248 312 344 4593
Size: 216 32 64 32 4249
Fragments arranged by size:
4249 216 64 32 32
XbaI T'CTAG_A
Cuts at: 0 2817 4593
Size: 2817 1776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 531 2307 3494 4593
Size: 531 1776 1187 1099
Fragments arranged by size:
1776 1187 1099 531
XmnI GAAnn'nnTTC
Cuts at: 0 565 2813 4593
Size: 565 2248 1780
Fragments arranged by size:
2248 1780 565
Enzymes that do cut:
Acc65I AcuI AflIII AgeI AhdI AlwNI ApoI AseI
AvaI AvrII BaeI BamHI BanI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaAI
BsaBI BsaWI BseRI BseYI BsmI Bsp1286I BspEI BsrDI
BsrFI BsrGI BssSI BstBI BstXI BstYI Bsu36I BtgI
BtsI DraI DraIII EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HincII HindIII HpaI KpnI MfeI
MmeI MslI MspA1I NcoI NdeI NspI PacI PciI
PpuMI PstI PvuII SanDI SapI SexAI SfcI SmlI
SpeI SspI StuI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AleI AlfI
AloI ApaI ApaLI AscI AsiSI BanII BbeI BbvCI
BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaI BsaHI
BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI BspHI BspMI
BsrBI BssHII BstAPI BstEII BstZ17I BtgZI ClaI CspCI
DrdI EaeI EagI EcoICRI EcoRV FseI FspI FspAI
HaeII KasI MluI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI Tth111I XhoI
XmaI ZraI