(Circular) (Six-base) MAPSORT of: pFN21AE2040.seq Check: 289 from: 1 to: 6658 pFN21AE2040 6658 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4468 278 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3546 3752 4001 1937 Size: 1609 206 249 4594 Fragments arranged by size: 4594 1609 249 206 Acc65I G'GTAC_C Cuts at: 3979 3979 Size: 6658 AclI AA'CG_TT Cuts at: 1563 4785 5158 1563 Size: 3222 373 3063 Fragments arranged by size: 3222 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2870 4721 6105 6373 1632 Size: 1238 1851 1384 268 1917 Fragments arranged by size: 1917 1851 1384 1238 268 AfeI AGC'GCT Cuts at: 2492 2492 Size: 6658 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6461 AflIII A'CryG_T Cuts at: 1203 3053 5557 6534 1203 Size: 1850 2504 977 1327 Fragments arranged by size: 2504 1850 1327 977 AgeI A'CCGG_T Cuts at: 5489 5489 Size: 6658 AhdI GACnn_n'nnGTC Cuts at: 5387 5387 Size: 6658 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6658 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6658 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2352 2384 2352 Size: 32 6626 AlwNI CAG_nnn'CTG Cuts at: 4401 5973 4401 Size: 1572 5086 ApaI G_GGCC'C Cuts at: 3049 3049 Size: 6658 ApaLI G'TGCA_C Cuts at: 4715 5871 4715 Size: 1156 5502 ApoI r'AATT_y Cuts at: 1493 3967 4193 4345 6454 1493 Size: 2474 226 152 2109 1697 Fragments arranged by size: 2474 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2754 3561 5212 160 Size: 872 1722 807 1651 1606 Fragments arranged by size: 1722 1651 1606 872 807 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 AvaI C'yCGr_G Cuts at: 1870 1943 3983 1870 Size: 73 2040 4545 Fragments arranged by size: 4545 2040 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2772 2805 2772 Size: 33 6625 BamHI G'GATC_C Cuts at: 2959 3988 4355 2959 Size: 1029 367 5262 Fragments arranged by size: 5262 1029 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3244 3979 5434 618 Size: 325 217 627 1457 735 1455 1842 Fragments arranged by size: 1842 1457 1455 735 627 325 217 BanII G_rGCy'C Cuts at: 1391 3049 3317 3977 1391 Size: 1658 268 660 4072 Fragments arranged by size: 4072 1658 660 268 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3488 928 Size: 971 1589 4098 Fragments arranged by size: 4098 1589 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4849 4883 1903 Size: 34 2912 34 3678 Fragments arranged by size: 3678 2912 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3529 4550 5760 3529 Size: 1021 1210 4427 Fragments arranged by size: 4427 1210 1021 BclI T'GATC_A Cuts at: 1548 3715 3775 1548 Size: 2167 60 4431 Fragments arranged by size: 4431 2167 60 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5269 136 Size: 107 122 71 631 605 3597 1525 Fragments arranged by size: 3597 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6653 6653 Size: 6658 BlpI GC'TnA_GC Cuts at: 4075 4075 Size: 6658 Bme1580I G_kGCm'C Cuts at: 1790 3049 3247 4719 5875 1790 Size: 1259 198 1472 1156 2573 Fragments arranged by size: 2573 1472 1259 1156 198 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6658 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2390 4389 5347 Size: 416 53 83 399 24 973 1999 958 Cuts at: 5347 442 Size: 1753 Fragments arranged by size: 1999 1753 973 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6658 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3498 5318 1239 Size: 2259 1820 2579 Fragments arranged by size: 2579 2259 1820 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3331 4783 5648 5946 3331 Size: 1452 865 298 4043 Fragments arranged by size: 4043 1452 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5321 882 Size: 617 3822 2219 Fragments arranged by size: 3822 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4449 493 Size: 711 3245 2702 Fragments arranged by size: 3245 2702 711 BsaBI GATnn'nnATC Cuts at: 6652 6652 Size: 6658 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2216 4465 4847 5482 Size: 53 83 186 936 683 2249 382 635 Cuts at: 5482 275 Size: 1451 Fragments arranged by size: 2249 1451 936 683 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2404 2917 5091 5489 5763 5910 6321 2404 Size: 513 2174 398 274 147 411 2741 Fragments arranged by size: 2741 2174 513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2257 2287 2352 2382 3662 Size: 30 165 30 854 30 65 30 1280 Cuts at: 3662 3692 1178 Size: 30 4144 Fragments arranged by size: 4144 1280 854 165 65 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3748 1186 Size: 2562 4096 BseYI C'CCAG_C Cuts at: 2741 5861 6357 2741 Size: 3120 496 3042 Fragments arranged by size: 3120 3042 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2558 3807 1163 Size: 573 822 1249 4014 Fragments arranged by size: 4014 1249 822 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4108 4869 5897 1540 Size: 275 186 2107 761 1028 2301 Fragments arranged by size: 2301 2107 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3317 4719 4804 5875 3317 Size: 1402 85 1071 4100 Fragments arranged by size: 4100 1402 1071 85 BsmI GAATG_Cn' Cuts at: 4169 4262 4169 Size: 93 6565 BsmBI CGTCTCn'nnnn_ Cuts at: 3345 3741 3345 Size: 396 6262 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3049 3247 3317 3977 4719 4804 5875 Size: 399 1259 198 70 660 742 85 1071 Cuts at: 5875 1391 Size: 2174 Fragments arranged by size: 2174 1259 1071 742 660 399 198 85 70 BspEI T'CCGG_A Cuts at: 2404 2917 6321 2404 Size: 513 3404 2741 Fragments arranged by size: 3404 2741 513 BspHI T'CATG_A Cuts at: 2211 4547 6277 2211 Size: 2336 1730 2592 Fragments arranged by size: 2592 2336 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4013 844 Size: 3169 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4545 1394 Size: 429 2722 3507 Fragments arranged by size: 3507 2722 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5153 5327 65 Size: 1183 3905 174 1396 Fragments arranged by size: 3905 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5302 5489 1700 Size: 110 3492 187 2869 Fragments arranged by size: 3492 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3200 96 Size: 3104 3554 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6658 BssSI C'ACGA_G Cuts at: 4718 5730 4718 Size: 1012 5646 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6658 BstBI TT'CG_AA Cuts at: 5462 5462 Size: 6658 BstEII G'GTnAC_C Cuts at: 1184 2306 2699 1184 Size: 1122 393 5143 Fragments arranged by size: 5143 1122 393 BstYI r'GATC_y Cuts at: 1972 2959 3988 4355 4740 4757 6209 6295 6307 Size: 987 1029 367 385 17 1452 86 12 Cuts at: 6307 6653 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1029 987 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3753 3753 Size: 6658 Bsu36I CC'TnA_GG Cuts at: 2990 3866 2990 Size: 876 5782 BtgI C'CryG_G Cuts at: 513 1065 2004 2556 2841 513 Size: 552 939 552 285 4330 Fragments arranged by size: 4330 939 552 552 285 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2304 487 Size: 683 56 1078 4841 Fragments arranged by size: 4841 1078 683 56 BtsI GCAGTG_nn' Cuts at: 4174 4968 4988 4174 Size: 794 20 5844 Fragments arranged by size: 5844 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6658 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6623 DraI TTT'AAA Cuts at: 3962 3962 Size: 6658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5665 809 Size: 507 4349 1802 Fragments arranged by size: 4349 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4105 4994 6623 Size: 54 1425 50 275 18 2275 889 1629 Cuts at: 6623 8 Size: 43 Fragments arranged by size: 2275 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4105 1537 Size: 275 2293 4090 Fragments arranged by size: 4090 2293 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2346 2394 3408 3417 4588 1353 Size: 993 48 1014 9 1171 3423 Fragments arranged by size: 3423 1171 1014 993 48 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5245 5619 5765 387 Size: 1064 138 435 3221 374 146 1280 Fragments arranged by size: 3221 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 3315 3315 Size: 6658 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2870 3498 4721 5318 6105 6373 1239 Size: 393 1238 628 1223 597 787 268 1524 Fragments arranged by size: 1524 1238 1223 787 628 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2348 3046 3441 2348 Size: 698 395 5565 Fragments arranged by size: 5565 698 395 EcoRI G'AATT_C Cuts at: 3967 4345 3967 Size: 378 6280 FspI TGC'GCA Cuts at: 5164 5164 Size: 6658 HaeII r_GCGC'y Cuts at: 1678 2494 5805 1678 Size: 816 3311 2531 Fragments arranged by size: 3311 2531 816 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2944 2976 3389 3421 3430 Size: 32 135 32 1372 32 413 32 9 Cuts at: 3430 3462 3902 3934 5299 5331 5373 5405 1373 Size: 32 440 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1372 1365 440 413 135 42 32 32 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 2952 4002 4248 669 Size: 988 281 1014 1050 246 3079 Fragments arranged by size: 3079 1050 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6658 HpaI GTT'AAC Cuts at: 4248 4248 Size: 6658 KpnI G_GTAC'C Cuts at: 3983 3983 Size: 6658 MfeI C'AATT_G Cuts at: 4257 4257 Size: 6658 MluI A'CGCG_T Cuts at: 6534 6534 Size: 6658 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2797 3882 5772 5956 1305 Size: 1492 1085 1890 184 2007 Fragments arranged by size: 2007 1890 1492 1085 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4836 Fragments arranged by size: 4836 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3095 4616 4975 5134 518 Size: 648 1929 1521 359 159 2042 Fragments arranged by size: 2042 1929 1521 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3692 4074 4751 5899 6144 1606 Size: 2086 382 677 1148 245 2120 Fragments arranged by size: 2120 2086 1148 677 382 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2556 2841 513 Size: 552 939 552 285 4330 Fragments arranged by size: 4330 939 552 552 285 NdeI CA'TA_TG Cuts at: 387 3151 387 Size: 2764 3894 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6658 NotI GC'GGCC_GC Cuts at: 4105 4105 Size: 6658 NspI r_CATG'y Cuts at: 1132 1229 3057 4016 5561 1132 Size: 97 1828 959 1545 2229 Fragments arranged by size: 2229 1828 1545 959 97 PacI TTA_AT'TAA Cuts at: 2940 2940 Size: 6658 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6658 PciI A'CATG_T Cuts at: 3053 5557 3053 Size: 2504 4154 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6658 PmeI GTTT'AAAC Cuts at: 3962 3962 Size: 6658 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2352 2384 4727 4759 6240 6272 2352 Size: 32 2343 32 1481 32 2738 Fragments arranged by size: 2738 2343 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2348 3441 2348 Size: 1093 5565 PshAI GACnn'nnGTC Cuts at: 1613 2484 1613 Size: 871 5787 PsiI TTA'TAA Cuts at: 4228 4228 Size: 6658 PspOMI G'GGCC_C Cuts at: 3045 3045 Size: 6658 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2049 2081 2049 Size: 32 6626 PstI C_TGCA'G Cuts at: 830 2224 2821 3697 4010 830 Size: 1394 597 876 313 3478 Fragments arranged by size: 3478 1394 876 597 313 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6658 SacI G_AGCT'C Cuts at: 3317 3317 Size: 6658 SalI G'TCGA_C Cuts at: 1936 4000 1936 Size: 2064 4594 SapI GCTCTTCn'nnn_ Cuts at: 2394 3417 2394 Size: 1023 5635 SbfI CC_TGCA'GG Cuts at: 4010 4010 Size: 6658 ScaI AGT'ACT Cuts at: 2067 3197 3799 4906 6618 2067 Size: 1130 602 1107 1712 2107 Fragments arranged by size: 2107 1712 1130 1107 602 SfcI C'TryA_G Cuts at: 826 1045 2149 2185 2220 2817 3543 3583 3693 Size: 219 1104 36 35 597 726 40 110 Cuts at: 3693 4006 5141 5822 6013 826 Size: 313 1135 681 191 1471 Fragments arranged by size: 1471 1135 1104 726 681 597 313 219 191 110 40 36 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6658 SmaI CCC'GGG Cuts at: 1872 3985 1872 Size: 2113 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3310 4762 5663 5925 820 Size: 197 926 1367 1452 901 262 1553 Fragments arranged by size: 1553 1452 1367 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4449 493 Size: 3956 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6658 SphI G_CATG'C Cuts at: 1132 4016 1132 Size: 2884 3774 SspI AAT'ATT Cuts at: 5 52 4582 5 Size: 47 4530 2081 Fragments arranged by size: 4530 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6658 StyI C'CwwG_G Cuts at: 513 1065 2004 2556 2841 4097 513 Size: 552 939 552 285 1256 3074 Fragments arranged by size: 3074 1256 939 552 552 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2409 2668 3622 4685 4702 4855 5040 Size: 1255 829 259 954 1063 17 153 185 Cuts at: 5040 5485 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1063 954 829 445 259 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2065 2590 3195 Size: 274 80 33 51 1443 88 525 605 Cuts at: 3195 3200 3268 3797 4904 6616 96 Size: 5 68 529 1107 1712 138 Fragments arranged by size: 1712 1443 1107 605 529 525 274 138 88 80 68 51 33 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2563 2595 3335 3367 6618 6650 2563 Size: 32 740 32 3251 32 2571 Fragments arranged by size: 3251 2571 740 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6658 XbaI T'CTAG_A Cuts at: 2927 3994 2927 Size: 1067 5591 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6658 XmaI C'CCGG_G Cuts at: 1870 3983 1870 Size: 2113 4545 XmnI GAAnn'nnTTC Cuts at: 2836 4787 2836 Size: 1951 4707 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4466 276 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PspXI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI