(Linear) (Six-base) MAPSORT of: KIEE2040.seq Check: 5044 from: 1 to: 1953 KIEE2040.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AccI GT'mk_AC Cuts at: 0 1541 1747 1953 Size: 1541 206 206 Fragments arranged by size: 1541 206 206 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 865 1953 Size: 865 1088 AfeI AGC'GCT Cuts at: 0 487 1953 Size: 487 1466 AflIII A'CryG_T Cuts at: 0 1048 1953 Size: 1048 905 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 347 379 1953 Size: 347 32 1574 Fragments arranged by size: 1574 347 32 ApaI G_GGCC'C Cuts at: 0 1044 1953 Size: 1044 909 AseI AT'TA_AT Cuts at: 0 749 1556 1953 Size: 749 807 397 Fragments arranged by size: 807 749 397 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 767 800 1953 Size: 767 33 1153 Fragments arranged by size: 1153 767 33 BamHI G'GATC_C Cuts at: 0 954 1953 Size: 954 999 BanI G'GyrC_C Cuts at: 0 1239 1953 Size: 1239 714 BanII G_rGCy'C Cuts at: 0 1044 1312 1953 Size: 1044 268 641 Fragments arranged by size: 1044 641 268 BbsI GAAGACnn'nnnn_ Cuts at: 0 1483 1953 Size: 1483 470 BciVI GTATCCnnnnn_n' Cuts at: 0 1524 1953 Size: 1524 429 BclI T'GATC_A Cuts at: 0 1710 1770 1953 Size: 1710 60 183 Fragments arranged by size: 1710 183 60 Bme1580I G_kGCm'C Cuts at: 0 1044 1242 1953 Size: 1044 198 711 Fragments arranged by size: 1044 711 198 BmrI ACTGGGnnnn_n' Cuts at: 0 385 1953 Size: 385 1568 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1493 1953 Size: 1493 460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1326 1953 Size: 1326 627 BsaHI Gr'CG_yC Cuts at: 0 211 1953 Size: 211 1742 BsaWI w'CCGG_w Cuts at: 0 399 912 1953 Size: 399 513 1041 Fragments arranged by size: 1041 513 399 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 252 282 347 377 1657 1687 1953 Size: 252 30 65 30 1280 30 266 Fragments arranged by size: 1280 266 252 65 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1743 1953 Size: 1743 210 BseYI C'CCAG_C Cuts at: 0 736 1953 Size: 736 1217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 553 1802 1953 Size: 553 1249 151 Fragments arranged by size: 1249 553 151 BsiHKAI G_wGCw'C Cuts at: 0 1312 1953 Size: 1312 641 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1340 1736 1953 Size: 1340 396 217 Fragments arranged by size: 1340 396 217 Bsp1286I G_dGCh'C Cuts at: 0 1044 1242 1312 1953 Size: 1044 198 70 641 Fragments arranged by size: 1044 641 198 70 BspEI T'CCGG_A Cuts at: 0 399 912 1953 Size: 399 513 1041 Fragments arranged by size: 1041 513 399 BspHI T'CATG_A Cuts at: 0 206 1953 Size: 206 1747 BsrGI T'GTAC_A Cuts at: 0 1195 1953 Size: 1195 758 BstEII G'GTnAC_C Cuts at: 0 301 694 1953 Size: 301 393 1259 Fragments arranged by size: 1259 393 301 BstYI r'GATC_y Cuts at: 0 954 1953 Size: 954 999 BstZ17I GTA'TAC Cuts at: 0 1748 1953 Size: 1748 205 Bsu36I CC'TnA_GG Cuts at: 0 985 1861 1953 Size: 985 876 92 Fragments arranged by size: 985 876 92 BtgI C'CryG_G Cuts at: 0 551 836 1953 Size: 551 285 1117 Fragments arranged by size: 1117 551 285 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 299 1953 Size: 299 1654 EarI CTCTTCn'nnn_ Cuts at: 0 341 389 1403 1412 1953 Size: 341 48 1014 9 541 Fragments arranged by size: 1014 541 341 48 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 1953 Size: 19 1934 EcoICRI GAG'CTC Cuts at: 0 1310 1953 Size: 1310 643 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 865 1493 1953 Size: 865 628 460 Fragments arranged by size: 865 628 460 EcoO109I rG'GnC_Cy Cuts at: 0 343 1041 1436 1953 Size: 343 698 395 517 Fragments arranged by size: 698 517 395 343 HaeII r_GCGC'y Cuts at: 0 489 1953 Size: 489 1464 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 939 971 1384 1416 1425 1457 1897 1929 Size: 939 32 413 32 9 32 440 32 Cuts at: 1929 1953 Size: 24 Fragments arranged by size: 939 440 413 32 32 32 32 24 9 HincII GTy'rAC Cuts at: 0 947 1953 Size: 947 1006 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 792 1877 1953 Size: 792 1085 76 Fragments arranged by size: 1085 792 76 MslI CAynn'nnrTG Cuts at: 0 1090 1953 Size: 1090 863 MspA1I CmG'CkG Cuts at: 0 1687 1953 Size: 1687 266 NcoI C'CATG_G Cuts at: 0 551 836 1953 Size: 551 285 1117 Fragments arranged by size: 1117 551 285 NdeI CA'TA_TG Cuts at: 0 1146 1953 Size: 1146 807 NspI r_CATG'y Cuts at: 0 1052 1953 Size: 1052 901 PacI TTA_AT'TAA Cuts at: 0 935 1953 Size: 935 1018 PciI A'CATG_T Cuts at: 0 1048 1953 Size: 1048 905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 347 379 1953 Size: 347 32 1574 Fragments arranged by size: 1574 347 32 PpuMI rG'GwC_Cy Cuts at: 0 343 1436 1953 Size: 343 1093 517 Fragments arranged by size: 1093 517 343 PshAI GACnn'nnGTC Cuts at: 0 479 1953 Size: 479 1474 PspOMI G'GGCC_C Cuts at: 0 1040 1953 Size: 1040 913 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 44 76 1953 Size: 44 32 1877 Fragments arranged by size: 1877 44 32 PstI C_TGCA'G Cuts at: 0 219 816 1692 1953 Size: 219 597 876 261 Fragments arranged by size: 876 597 261 219 SacI G_AGCT'C Cuts at: 0 1312 1953 Size: 1312 641 SapI GCTCTTCn'nnn_ Cuts at: 0 389 1412 1953 Size: 389 1023 541 Fragments arranged by size: 1023 541 389 ScaI AGT'ACT Cuts at: 0 62 1192 1794 1953 Size: 62 1130 602 159 Fragments arranged by size: 1130 602 159 62 SfcI C'TryA_G Cuts at: 0 144 180 215 812 1538 1578 1688 1953 Size: 144 36 35 597 726 40 110 265 Fragments arranged by size: 726 597 265 144 110 40 36 35 SmlI C'TyrA_G Cuts at: 0 1305 1953 Size: 1305 648 StyI C'CwwG_G Cuts at: 0 551 836 1953 Size: 551 285 1117 Fragments arranged by size: 1117 551 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 404 663 1617 1953 Size: 404 259 954 336 Fragments arranged by size: 954 404 336 259 TatI w'GTAC_w Cuts at: 0 60 585 1190 1195 1263 1792 1953 Size: 60 525 605 5 68 529 161 Fragments arranged by size: 605 529 525 161 68 60 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 558 590 1330 1362 1953 Size: 558 32 740 32 591 Fragments arranged by size: 740 591 558 32 32 XbaI T'CTAG_A Cuts at: 0 922 1953 Size: 922 1031 XmnI GAAnn'nnTTC Cuts at: 0 831 1953 Size: 831 1122 Enzymes that do cut: AccI AcuI AfeI AflIII AloI ApaI AseI BaeI BamHI BanI BanII BbsI BciVI BclI Bme1580I BmrI BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrGI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII MmeI MslI MspA1I NcoI NdeI NspI PacI PciI PpiI PpuMI PshAI PspOMI PsrI PstI SacI SapI ScaI SfcI SmlI StyI TaqII TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AsiSI AvaI AvrII BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsiEI BsiWI BsmI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PasI PflMI PfoI PmeI PmlI PsiI PspXI PvuI PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XcmI XhoI XmaI ZraI