################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvrII BalI BamHI BbeI BbrPI BciVI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FseI HgaI HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 1188 GACGTC GACGT^C AccI 604 GTCTAC GT^[AC][GT]AC AcsI 568 AAATTT [GA]^AATT[CT] AcyI 1188 GACGTC G[GA]^CG[CT]C AhdI 1276 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 152 GGATC GGATC(4/5) ApaI 337 GGGCCC GGGCC^C ApoI 568 AAATTT [GA]^AATT[CT] Asp700I 319 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1276 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 196 CCCGGG C^[CT]CG[GA]G AviII 549 TGCGCA TGC^GCA BanI 475 GGCACC G^G[CT][GA]CC BbvCI 906 CCTCAGC CCTCAGC(-5/-2) BcgI 1183 GCAGGGACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 32 TGATCA T^GATCA BglII 380 AGATCT A^GATCT BsaHI 1188 GACGTC G[GA]^CG[CT]C BsaI 121 GAGACC GGTCTC(1/5) BsoBI 196 CCCGGG C^[CT]CG[GA]G BsrFI 1356 GCCGGT [GA]^CCGG[CT] BstEII 879 GGTCACC G^GT[ACGT]ACC BstPI 879 GGTCACC G^GT[ACGT]ACC Cfr10I 1356 GCCGGT [GA]^CCGG[CT] DraII 336 GGGGCCC [GA]G^G[ACGT]CC[CT] DrdI 232 GACATCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1276 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1225 GAAGAG CTCTTC(1/4) EcoO109I 336 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 879 GGTCACC G^GT[ACGT]ACC FbaI 32 TGATCA T^GATCA FspI 549 TGCGCA TGC^GCA Hin1I 1188 GACGTC G[GA]^CG[CT]C HincII 1116 GTCAAC GT[CT]^[GA]AC HindII 1116 GTCAAC GT[CT]^[GA]AC Ksp632I 1225 GAAGAG CTCTTC(1/4) MseI 332 TTAA T^TAA NcoI 956 CCATGG C^CATGG PstI 509 CTGCAG CTGCA^G SfcI 509 CTGCAG C^T[GA][CT]AG SmaI 196 CCCGGG CCC^GGG SmlI 529 CTTGAG C^T[CT][GA]AG TaqI 1192 TCGA T^CGA Tru9I 332 TTAA T^TAA TthHB8I 1192 TCGA T^CGA XmaCI 196 CCCGGG C^CCGGG XmaI 196 CCCGGG C^CCGGG XmnI 319 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflIII 290 ACGTGT A^C[GA][CT]GT 395 ACACGT BanII 163 GGGCTC G[GA]GC[CT]^C 337 GGGCCC BbsI 1230 GAAGAC GAAGAC(2/6) 991 GTCTTC BpmI 657 CTCCAG CTGGAG(16/14) 463 CTGGAG BpuAI 1230 GAAGAC GAAGAC(2/6) 991 GTCTTC BspHI 29 TCATGA T^CATGA 737 TCATGA BstYI 152 GGATCT [GA]^GATC[CT] 380 AGATCT EcoT14I 956 CCATGG C^C[AT][AT]GG 1067 CCTTGG HinfI 850 GAATC G^A[ACGT]TC 1289 GAATC MflI 152 GGATCT [GA]^GATC[CT] 380 AGATCT PflMI 675 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 956 CCATGGACTGG RcaI 29 TCATGA T^CATGA 737 TCATGA StyI 956 CCATGG C^C[AT][AT]GG 1067 CCTTGG TfiI 850 GAATC G^A[AT]TC 1289 GAATC TspRI 110 CAGTG CA[GC]TG[ACGT][ACGT]^ 699 CACTG Van91I 675 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 956 CCATGGACTGG XcmI 626 CCACCAAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 629 CCAAGTCGGTGGTGG XhoII 152 GGATCT [GA]^GATC[CT] 380 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I