(Circular) (Six-base) MAPSORT of: pFN21AE4447.seq Check: 1570 from: 1 to: 6091 pFN21AE4447 6091 bp With 182 enzymes: SgfI * March 28, 2012 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3197 3901 278 Size: 53 83 186 936 1661 704 2468 Fragments arranged by size: 2468 1661 936 704 186 83 53 AccI GT'mk_AC Cuts at: 1937 2610 3434 1937 Size: 673 824 4594 Fragments arranged by size: 4594 824 673 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2622 3249 4154 5538 5806 1632 Size: 990 627 905 1384 268 1917 Fragments arranged by size: 1917 1384 990 905 627 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 2295 2400 4990 5967 1203 Size: 1092 105 2590 977 1327 Fragments arranged by size: 2590 1327 1092 977 105 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 3286 4820 3286 Size: 1534 4557 AleI CACnn'nnGTG Cuts at: 1166 2225 2891 1166 Size: 1059 666 4366 Fragments arranged by size: 4366 1059 666 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 3834 5406 3834 Size: 1572 4519 ApaI G_GGCC'C Cuts at: 2346 2346 Size: 6091 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2573 3400 3626 3778 5887 1493 Size: 1080 827 226 152 2109 1697 Fragments arranged by size: 2109 1697 1080 827 226 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2201 3416 1870 Size: 73 258 1215 4545 Fragments arranged by size: 4545 1215 258 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3250 3283 3250 Size: 33 6058 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2480 3412 4867 618 Size: 325 217 627 693 932 1455 1842 Fragments arranged by size: 1842 1455 932 693 627 325 217 BanII G_rGCy'C Cuts at: 1391 2172 2346 3410 1391 Size: 781 174 1064 4072 Fragments arranged by size: 4072 1064 781 174 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2989 3242 928 Size: 971 1090 253 3777 Fragments arranged by size: 3777 1090 971 253 BbvCI CC'TCA_GC Cuts at: 2912 2912 Size: 6091 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3177 3211 4282 4316 1903 Size: 34 1240 34 1071 34 3678 Fragments arranged by size: 3678 1240 1071 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 2037 1548 Size: 489 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2385 6086 2385 Size: 3701 2390 BlpI GC'TnA_GC Cuts at: 3508 3508 Size: 6091 Bme1580I G_kGCm'C Cuts at: 1790 2346 2525 2705 4152 5308 1790 Size: 556 179 180 1447 1156 2573 Fragments arranged by size: 2573 1447 1156 556 180 179 BmgBI CAC'GTC Cuts at: 1318 2296 1318 Size: 978 5113 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3150 3299 3346 Size: 416 53 83 399 24 1733 149 47 Cuts at: 3346 3822 4780 442 Size: 476 958 1753 Fragments arranged by size: 1753 1733 958 476 416 399 149 83 53 47 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2489 2647 4751 1239 Size: 1250 158 2104 2579 Fragments arranged by size: 2579 2104 1250 158 Bpu10I CC'TnA_GC Cuts at: 2912 2912 Size: 6091 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2555 4216 5081 5379 2555 Size: 1661 865 298 3267 Fragments arranged by size: 3267 1661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2120 4754 882 Size: 617 621 2634 2219 Fragments arranged by size: 2634 2219 621 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3194 3898 4280 4915 Size: 53 83 186 936 1661 704 382 635 Cuts at: 4915 275 Size: 1451 Fragments arranged by size: 1661 1451 936 704 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5866 Fragments arranged by size: 5866 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6091 BseYI C'CCAG_C Cuts at: 2206 2863 3048 3184 5294 5790 2206 Size: 657 185 136 2110 496 2507 Fragments arranged by size: 2507 2110 657 496 185 136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5518 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4152 4237 5308 4152 Size: 85 1071 4935 Fragments arranged by size: 4935 1071 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2346 2525 2705 3410 4152 4237 Size: 399 382 174 179 180 705 742 85 Cuts at: 4237 5308 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 705 399 382 180 179 174 85 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 2034 2742 3980 5710 2034 Size: 708 1238 1730 2415 Fragments arranged by size: 2415 1730 1238 708 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3446 844 Size: 2602 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4586 4760 65 Size: 1183 3338 174 1396 Fragments arranged by size: 3338 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3361 4735 4922 1700 Size: 110 1551 1374 187 2869 Fragments arranged by size: 2869 1551 1374 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 2884 1184 Size: 1700 4391 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2641 2962 2161 Size: 480 321 5290 Fragments arranged by size: 5290 480 321 BstYI r'GATC_y Cuts at: 1972 2157 2385 3421 3788 4173 4190 5642 5728 Size: 185 228 1036 367 385 17 1452 86 Cuts at: 5728 5740 6086 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1036 385 367 346 228 185 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2889 2961 3021 513 Size: 552 939 885 72 60 3583 Fragments arranged by size: 3583 939 885 552 72 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2413 487 Size: 683 56 1187 4165 Fragments arranged by size: 4165 1187 683 56 BtsI GCAGTG_nn' Cuts at: 3607 4401 4421 3607 Size: 794 20 5277 Fragments arranged by size: 5277 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2243 5098 809 Size: 507 927 2855 1802 Fragments arranged by size: 2855 1802 927 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3538 4427 6056 Size: 54 1425 50 275 18 1708 889 1629 Cuts at: 6056 8 Size: 43 Fragments arranged by size: 1708 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3225 4021 1353 Size: 1872 796 3423 Fragments arranged by size: 3423 1872 796 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2666 4678 5052 5198 387 Size: 1064 138 1077 2012 374 146 1280 Fragments arranged by size: 2012 1280 1077 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2489 2622 2647 3249 4154 4751 5538 Size: 393 857 133 25 602 905 597 787 Cuts at: 5538 5806 1239 Size: 268 1524 Fragments arranged by size: 1524 905 857 787 602 597 393 268 133 25 EcoO109I rG'GnC_Cy Cuts at: 2342 2342 Size: 6091 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FspI TGC'GCA Cuts at: 2556 4597 2556 Size: 2041 4050 FspAI rTGC'GCAy Cuts at: 2556 2556 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 2356 2925 3008 5238 1678 Size: 678 569 83 2230 2531 Fragments arranged by size: 2531 2230 678 569 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2202 2234 3272 3304 4732 Size: 32 135 32 630 32 1038 32 1428 Cuts at: 4732 4764 4806 4838 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1428 1038 630 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3123 3435 3681 669 Size: 988 281 1185 312 246 3079 Fragments arranged by size: 3079 1185 988 312 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2292 2329 5205 5389 1305 Size: 987 37 2876 184 2007 Fragments arranged by size: 2876 2007 987 184 37 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4269 Fragments arranged by size: 4269 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 2276 2294 2741 2891 2960 2978 Size: 648 1059 51 18 447 150 69 18 Cuts at: 2978 4049 4408 4567 518 Size: 1071 359 159 2042 Fragments arranged by size: 2042 1071 1059 648 447 359 159 150 69 51 18 18 MspA1I CmG'CkG Cuts at: 1606 3507 4184 5332 5577 1606 Size: 1901 677 1148 245 2120 Fragments arranged by size: 2120 1901 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2961 513 Size: 552 939 957 3643 Fragments arranged by size: 3643 957 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 3449 4994 1132 Size: 97 2220 1545 2229 Fragments arranged by size: 2229 2220 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 2686 2967 1108 Size: 1578 281 4232 Fragments arranged by size: 4232 1578 281 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PspOMI G'GGCC_C Cuts at: 2342 2342 Size: 6091 PstI C_TGCA'G Cuts at: 830 2518 3443 830 Size: 1688 925 3478 Fragments arranged by size: 3478 1688 925 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 2514 3439 4574 5255 5446 826 Size: 219 1469 925 1135 681 191 1471 Fragments arranged by size: 1471 1469 1135 925 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 2203 3418 1872 Size: 331 1215 4545 Fragments arranged by size: 4545 1215 331 SmlI C'TyrA_G Cuts at: 820 1017 1943 2534 4195 5096 5358 820 Size: 197 926 591 1661 901 262 1553 Fragments arranged by size: 1661 1553 926 901 591 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SrfI GCCC'GGGC Cuts at: 2203 2203 Size: 6091 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2004 2961 3072 3530 513 Size: 552 939 957 111 458 3074 Fragments arranged by size: 3074 957 939 552 458 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2249 4118 4135 4288 4473 4918 325 Size: 1255 669 1869 17 153 185 445 1498 Fragments arranged by size: 1869 1498 1255 669 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 4337 6049 Size: 274 80 33 51 1443 306 2054 1712 Cuts at: 6049 96 Size: 138 Fragments arranged by size: 2054 1712 1443 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 2641 2638 Size: 3 6088 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 2201 3416 1870 Size: 331 1215 4545 Fragments arranged by size: 4545 1215 331 XmnI GAAnn'nnTTC Cuts at: 2328 4220 2328 Size: 1892 4199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3195 3899 276 Size: 53 83 186 936 1661 704 2468 Fragments arranged by size: 2468 1661 936 704 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI