(Linear) (Six-base) MAPSORT of: KIEE4447.seq Check: 3713 from: 1 to: 1386 KIEE4447.orf With 182 enzymes: SgfI * March 22, 2012 18:03 .. AatII G_ACGT'C Cuts at: 0 1192 1386 Size: 1192 194 AccI GT'mk_AC Cuts at: 0 605 1386 Size: 605 781 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 617 1244 1386 Size: 617 627 142 Fragments arranged by size: 627 617 142 AflIII A'CryG_T Cuts at: 0 290 395 1386 Size: 290 105 991 Fragments arranged by size: 991 290 105 AhdI GACnn_n'nnGTC Cuts at: 0 1281 1386 Size: 1281 105 AleI CACnn'nnGTG Cuts at: 0 220 886 1386 Size: 220 666 500 Fragments arranged by size: 666 500 220 ApaI G_GGCC'C Cuts at: 0 341 1386 Size: 341 1045 ApoI r'AATT_y Cuts at: 0 568 1386 Size: 568 818 AvaI C'yCGr_G Cuts at: 0 196 1386 Size: 196 1190 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1245 1278 1386 Size: 1245 33 108 Fragments arranged by size: 1245 108 33 BanI G'GyrC_C Cuts at: 0 475 1386 Size: 475 911 BanII G_rGCy'C Cuts at: 0 167 341 1386 Size: 167 174 1045 Fragments arranged by size: 1045 174 167 BbsI GAAGACnn'nnnn_ Cuts at: 0 984 1237 1386 Size: 984 253 149 Fragments arranged by size: 984 253 149 BbvCI CC'TCA_GC Cuts at: 0 907 1386 Size: 907 479 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1172 1206 1386 Size: 1172 34 180 Fragments arranged by size: 1172 180 34 BclI T'GATC_A Cuts at: 0 32 1386 Size: 32 1354 BglII A'GATC_T Cuts at: 0 380 1386 Size: 380 1006 Bme1580I G_kGCm'C Cuts at: 0 341 520 700 1386 Size: 341 179 180 686 Fragments arranged by size: 686 341 180 179 BmgBI CAC'GTC Cuts at: 0 291 1386 Size: 291 1095 BmrI ACTGGGnnnn_n' Cuts at: 0 1145 1294 1341 1386 Size: 1145 149 47 45 Fragments arranged by size: 1145 149 47 45 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 642 1386 Size: 484 158 744 Fragments arranged by size: 744 484 158 Bpu10I CC'TnA_GC Cuts at: 0 907 1386 Size: 907 479 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 550 1386 Size: 550 836 BsaI GGTCTCn'nnnn_ Cuts at: 0 115 1386 Size: 115 1271 BsaHI Gr'CG_yC Cuts at: 0 1189 1386 Size: 1189 197 BseYI C'CCAG_C Cuts at: 0 201 858 1043 1179 1386 Size: 201 657 185 136 207 Fragments arranged by size: 657 207 201 185 136 Bsp1286I G_dGCh'C Cuts at: 0 167 341 520 700 1386 Size: 167 174 179 180 686 Fragments arranged by size: 686 180 179 174 167 BspHI T'CATG_A Cuts at: 0 29 737 1386 Size: 29 708 649 Fragments arranged by size: 708 649 29 BsrFI r'CCGG_y Cuts at: 0 1356 1386 Size: 1356 30 BstEII G'GTnAC_C Cuts at: 0 879 1386 Size: 879 507 BstXI CCAn_nnnn'nTGG Cuts at: 0 156 636 957 1386 Size: 156 480 321 429 Fragments arranged by size: 480 429 321 156 BstYI r'GATC_y Cuts at: 0 152 380 1386 Size: 152 228 1006 Fragments arranged by size: 1006 228 152 BtgI C'CryG_G Cuts at: 0 884 956 1016 1386 Size: 884 72 60 370 Fragments arranged by size: 884 370 72 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 408 1386 Size: 408 978 DrdI GACnn_nn'nnGTC Cuts at: 0 238 1386 Size: 238 1148 EarI CTCTTCn'nnn_ Cuts at: 0 1220 1386 Size: 1220 166 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 661 1386 Size: 661 725 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 617 642 1244 1386 Size: 484 133 25 602 142 Fragments arranged by size: 602 484 142 133 25 EcoO109I rG'GnC_Cy Cuts at: 0 337 1386 Size: 337 1049 FspI TGC'GCA Cuts at: 0 551 1386 Size: 551 835 FspAI rTGC'GCAy Cuts at: 0 551 1386 Size: 551 835 HaeII r_GCGC'y Cuts at: 0 351 920 1003 1386 Size: 351 569 83 383 Fragments arranged by size: 569 383 351 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 197 229 1267 1299 1386 Size: 197 32 1038 32 87 Fragments arranged by size: 1038 197 87 32 32 HincII GTy'rAC Cuts at: 0 1118 1386 Size: 1118 268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 287 324 1386 Size: 287 37 1062 Fragments arranged by size: 1062 287 37 MslI CAynn'nnrTG Cuts at: 0 220 271 289 736 886 955 973 1386 Size: 220 51 18 447 150 69 18 413 Fragments arranged by size: 447 413 220 150 69 51 18 18 NcoI C'CATG_G Cuts at: 0 956 1386 Size: 956 430 PflMI CCAn_nnn'nTGG Cuts at: 0 681 962 1386 Size: 681 281 424 Fragments arranged by size: 681 424 281 PspOMI G'GGCC_C Cuts at: 0 337 1386 Size: 337 1049 PstI C_TGCA'G Cuts at: 0 513 1386 Size: 513 873 SfcI C'TryA_G Cuts at: 0 509 1386 Size: 509 877 SmaI CCC'GGG Cuts at: 0 198 1386 Size: 198 1188 SmlI C'TyrA_G Cuts at: 0 529 1386 Size: 529 857 SrfI GCCC'GGGC Cuts at: 0 198 1386 Size: 198 1188 StyI C'CwwG_G Cuts at: 0 956 1067 1386 Size: 956 111 319 Fragments arranged by size: 956 319 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 244 1386 Size: 244 1142 TatI w'GTAC_w Cuts at: 0 278 1386 Size: 278 1108 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 633 636 1386 Size: 633 3 750 Fragments arranged by size: 750 633 3 XmaI C'CCGG_G Cuts at: 0 196 1386 Size: 196 1190 XmnI GAAnn'nnTTC Cuts at: 0 323 1386 Size: 323 1063 ZraI GAC'GTC Cuts at: 0 1190 1386 Size: 1190 196 Enzymes that do cut: AatII AccI AcuI AflIII AhdI AleI ApaI ApoI AvaI BaeI BanI BanII BbsI BbvCI BcgI BclI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BseYI Bsp1286I BspHI BsrFI BstEII BstXI BstYI BtgI BtgZI DrdI EarI EciI Eco57MI EcoO109I FspI FspAI HaeII Hin4I HincII MmeI MslI NcoI PflMI PspOMI PstI SfcI SmaI SmlI SrfI StyI TaqII TatI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BciVI BfrBI BglI BlpI BmtI BplI BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EaeI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I XbaI XhoI