################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AvaI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BpuAI BsaAI BsaBI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 999 CGCG CG^CG AcsI 152 AAATTC [GA]^AATT[CT] AcyI 639 GACGCC G[GA]^CG[CT]C AhdI 667 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1029 GGATC GGATC(4/5) ApaI 284 GGGCCC GGGCC^C ApoI 152 AAATTC [GA]^AATT[CT] AspEI 667 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 432 TGCGCA TGC^GCA BanI 1099 GGCACC G^G[CT][GA]CC BanII 284 GGGCCC G[GA]GC[CT]^C BbvI 939 GCTGC GCAGC(8/12) BciVI 709 GGATAC GTATCC(6/5) BcnI 1272 CCGGG CC^[GC]GG BglII 815 AGATCT A^GATCT BlpI 949 GCTCAGC GC^T[ACGT]AGC BmyI 284 GGGCCC G[AGT]GC[ACT]^C Bpu1102I 949 GCTCAGC GC^T[ACGT]AGC BsaHI 639 GACGCC G[GA]^CG[CT]C BsaI 440 GAGACC GGTCTC(1/5) BsaWI 864 ACCGGA [AT]^CCGG[AT] BsmI 1276 GAATGC GAATGC(1/-1) Bsp1286I 284 GGGCCC G[AGT]GC[ACT]^C BspHI 773 TCATGA T^CATGA BspMI 1374 GCAGGT ACCTGC(4/8) BsrDI 429 CATTGC GCAATG(2/0) BsrFI 350 ACCGGC [GA]^CCGG[CT] BstAPI 970 GCAGTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 483 GGATG GGATG(2/0) BstUI 999 CGCG CG^CG BstXI 1134 CCATGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 949 GCTCAGC GC^T[ACGT]AGC Cfr10I 350 ACCGGC [GA]^CCGG[CT] DraIII 1057 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 667 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FokI 483 GGATG GGATG(9/13) FspI 432 TGCGCA TGC^GCA HgaI 639 GACGC GACGC(5/10) Hin1I 639 GACGCC G[GA]^CG[CT]C MfeI 66 CAATTG C^AATTG MunI 66 CAATTG C^AATTG MvnI 999 CGCG CG^CG NciI 1272 CCGGG CC^[GC]GG PflFI 1114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 576 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1300 CTGCAG CTGCA^G RcaI 773 TCATGA T^CATGA SapI 1384 GAAGAGC GCTCTTC(1/4) SfcI 1300 CTGCAG C^T[GA][CT]AG TseI 939 GCTGC G^C[AT]GC Tth111I 1114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 576 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 187 GTAC GT^AC 580 GTAC AvrII 280 CCTAGG C^CTAGG 678 CCTAGG BalI 827 TGGCCA TGG^CCA 1010 TGGCCA BlnI 280 CCTAGG C^CTAGG 678 CCTAGG BpmI 583 CTGGAG CTGGAG(16/14) 1214 CTGGAG BseRI 7 GAGGAG GAGGAG(10/8) 868 GAGGAG BsgI 193 CTGCAC GTGCAG(16/14) 1054 CTGCAC BsmAI 440 GAGAC GTCTC(1/5) 873 GAGAC BsmFI 675 GTCCC GGGAC(10/14) 1398 GTCCC BstYI 815 AGATCT [GA]^GATC[CT] 1029 GGATCT DraII 283 AGGGCCC [GA]G^G[ACGT]CC[CT] 923 AGGCCCT EaeI 827 TGGCCA [CT]^GGCC[GA] 1010 TGGCCA EcoO109I 283 AGGGCCC [GA]G^G[ACGT]CC[CT] 923 AGGCCCT Fnu4HI 408 GCGGC GC^[ACGT]GC 939 GCTGC ItaI 408 GCGGC GC^[ACGT]GC 939 GCTGC MaeII 189 ACGT A^CGT 1396 ACGT MflI 815 AGATCT [GA]^GATC[CT] 1029 GGATCT MluNI 827 TGGCCA TGG^CCA 1010 TGGCCA MscI 827 TGGCCA TGG^CCA 1010 TGGCCA MslI 1129 CACGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1135 CATGAGAATG MspA1I 952 CAGCTG C[AC]G^C[GT]G 1311 CAGCTG MwoI 591 GCAAAGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 970 GCAGTGGTTGC PvuII 952 CAGCTG CAG^CTG 1311 CAGCTG RsaI 187 GTAC GT^AC 580 GTAC SmlI 131 CTCAAG C^T[CT][GA]AG 445 CTCAAG TaqI 884 TCGA T^CGA 1347 TCGA TthHB8I 884 TCGA T^CGA 1347 TCGA XhoII 815 AGATCT [GA]^GATC[CT] 1029 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AluI AlwNI AvaII BfaI BsaJI BsiYI BslI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I HaeIII HapII HhaI HinP1I HinfI HpaII HphI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspI MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I Tsp45I Tsp509I TspRI XcmI