(Circular) (Six-base) MAPSORT of: pFN21AE5678.seq Check: 2235 from: 1 to: 6118 pFN21AE5678 6118 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3370 3370 Size: 6118 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3461 1937 Size: 1524 4594 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2879 3298 4181 5565 5833 1632 Size: 1247 419 883 1384 268 1917 Fragments arranged by size: 1917 1384 1247 883 419 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 5017 5994 1203 Size: 3814 977 1327 Fragments arranged by size: 3814 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 2677 4847 2677 Size: 2170 3948 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AlwNI CAG_nnn'CTG Cuts at: 2086 2587 3037 3861 5433 2086 Size: 501 450 824 1572 2771 Fragments arranged by size: 2771 1572 824 501 450 ApaI G_GGCC'C Cuts at: 2293 2293 Size: 6118 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2157 3427 3653 3805 5914 1493 Size: 664 1270 226 152 2109 1697 Fragments arranged by size: 2109 1697 1270 664 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 3443 1870 Size: 73 1500 4545 Fragments arranged by size: 4545 1500 73 AvrII C'CTAG_G Cuts at: 2285 2683 2285 Size: 398 5720 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3104 3439 4894 618 Size: 325 217 627 1317 335 1455 1842 Fragments arranged by size: 1842 1455 1317 627 335 325 217 BanII G_rGCy'C Cuts at: 1391 2293 3437 1391 Size: 902 1144 4072 Fragments arranged by size: 4072 1144 902 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2708 4010 5220 2708 Size: 1302 1210 3606 Fragments arranged by size: 3606 1302 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4729 136 Size: 107 122 71 631 605 3057 1525 Fragments arranged by size: 3057 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2820 6113 2820 Size: 3293 2825 BlpI GC'TnA_GC Cuts at: 2955 3535 2955 Size: 580 5538 Bme1580I G_kGCm'C Cuts at: 1790 2293 4179 5335 1790 Size: 503 1886 1156 2573 Fragments arranged by size: 2573 1886 1156 503 BmgBI CAC'GTC Cuts at: 1318 3402 1318 Size: 2084 4034 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2489 2548 3849 Size: 416 53 83 399 24 1072 59 1301 Cuts at: 3849 4807 442 Size: 958 1753 Fragments arranged by size: 1753 1301 1072 958 416 399 83 59 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2609 3240 4778 1239 Size: 1370 631 1538 2579 Fragments arranged by size: 2579 1538 1370 631 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2121 2435 4243 5108 5406 2121 Size: 314 1808 865 298 2833 Fragments arranged by size: 2833 1808 865 314 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2439 4781 882 Size: 617 940 2342 2219 Fragments arranged by size: 2342 2219 940 617 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2645 3925 4307 4942 Size: 53 83 186 936 1112 1280 382 635 Cuts at: 4942 275 Size: 1451 Fragments arranged by size: 1451 1280 1112 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2869 4551 4949 5223 5370 5781 2869 Size: 1682 398 274 147 411 3206 Fragments arranged by size: 3206 1682 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5893 Fragments arranged by size: 5893 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2888 1186 Size: 841 861 4416 Fragments arranged by size: 4416 861 841 BseYI C'CCAG_C Cuts at: 2086 5321 5817 2086 Size: 3235 496 2387 Fragments arranged by size: 3235 2387 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 3044 1163 Size: 573 447 861 4237 Fragments arranged by size: 4237 861 573 447 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4179 4264 5335 4179 Size: 85 1071 4962 Fragments arranged by size: 4962 1071 85 BsmI GAATG_Cn' Cuts at: 3287 3629 3722 3287 Size: 342 93 5683 Fragments arranged by size: 5683 342 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2293 3437 4179 4264 5335 1391 Size: 399 503 1144 742 85 1071 2174 Fragments arranged by size: 2174 1144 1071 742 503 399 85 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 2778 4007 5737 2778 Size: 1229 1730 3159 Fragments arranged by size: 3159 1730 1229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3370 3473 844 Size: 2526 103 3489 Fragments arranged by size: 3489 2526 103 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2433 4613 4787 65 Size: 1183 1185 2180 174 1396 Fragments arranged by size: 2180 1396 1185 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2355 4762 4949 1700 Size: 110 545 2407 187 2869 Fragments arranged by size: 2869 2407 545 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 2981 794 Size: 2187 3931 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstXI CCAn_nnnn'nTGG Cuts at: 3146 3146 Size: 6118 BstYI r'GATC_y Cuts at: 1972 2820 3034 3448 3815 4200 4217 5669 5755 Size: 848 214 414 367 385 17 1452 86 Cuts at: 5755 5767 6113 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 848 414 385 367 346 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5566 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 2982 3634 4428 4448 2982 Size: 652 794 20 4652 Fragments arranged by size: 4652 794 652 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DraIII CAC_nnn'GTG Cuts at: 3067 3067 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2832 3015 3565 Size: 54 1425 50 275 18 1002 183 550 Cuts at: 3565 4454 6083 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1002 889 550 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2802 3083 3384 4048 1353 Size: 1449 281 301 664 3423 Fragments arranged by size: 3423 1449 664 301 281 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2609 2879 3240 3298 4181 4778 5565 Size: 393 977 270 361 58 883 597 787 Cuts at: 5565 5833 1239 Size: 268 1524 Fragments arranged by size: 1524 977 883 787 597 393 361 270 268 58 EcoO109I rG'GnC_Cy Cuts at: 2289 2929 2289 Size: 640 5478 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 FspI TGC'GCA Cuts at: 2439 4624 2439 Size: 2185 3933 HaeII r_GCGC'y Cuts at: 1678 5265 1678 Size: 3587 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2663 2695 2871 2903 4759 Size: 32 135 32 1091 32 176 32 1856 Cuts at: 4759 4791 4833 4865 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1856 1091 176 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3708 669 Size: 988 281 1524 246 3079 Fragments arranged by size: 3079 1524 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6118 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 2071 3717 2071 Size: 1646 4472 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 5232 5416 1305 Size: 684 3243 184 2007 Fragments arranged by size: 3243 2007 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2834 3017 10 Size: 54 1425 343 1002 183 3111 Fragments arranged by size: 3111 1425 1002 343 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 3138 3144 4076 4435 4594 518 Size: 648 1972 6 932 359 159 2042 Fragments arranged by size: 2042 1972 932 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2959 3318 3534 4211 5359 5604 1606 Size: 1353 359 216 677 1148 245 2120 Fragments arranged by size: 2120 1353 1148 677 359 245 216 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5566 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 3476 5021 1132 Size: 97 2247 1545 2229 Fragments arranged by size: 2247 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 2587 1108 Size: 1479 4639 PfoI T'CCnGG_A Cuts at: 3030 3030 Size: 6118 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PspOMI G'GGCC_C Cuts at: 2289 2289 Size: 6118 PstI C_TGCA'G Cuts at: 830 3309 3470 830 Size: 2479 161 3478 Fragments arranged by size: 3478 2479 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 2959 3318 2959 Size: 359 5759 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SapI GCTCTTCn'nnn_ Cuts at: 3384 3384 Size: 6118 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SfcI C'TryA_G Cuts at: 826 1045 3305 3466 4601 5282 5473 826 Size: 219 2260 161 1135 681 191 1471 Fragments arranged by size: 2260 1471 1135 681 219 191 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2136 2450 4222 5123 5385 820 Size: 197 926 193 314 1772 901 262 1553 Fragments arranged by size: 1772 1553 926 901 314 262 197 193 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6118 StyI C'CwwG_G Cuts at: 513 1065 2285 2533 2683 3171 3557 513 Size: 552 1220 248 150 488 386 3074 Fragments arranged by size: 3074 1220 552 488 386 248 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2235 4145 4162 4315 4500 4945 325 Size: 1255 655 1910 17 153 185 445 1498 Fragments arranged by size: 1910 1498 1255 655 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4364 6076 96 Size: 274 80 33 51 1443 2387 1712 138 Fragments arranged by size: 2387 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3313 3345 6078 6110 3313 Size: 32 2733 32 3321 Fragments arranged by size: 3321 2733 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3122 1316 Size: 1806 4312 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2808 2929 3169 2808 Size: 121 240 5757 Fragments arranged by size: 5757 240 121 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 4247 4247 Size: 6118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI