(Linear) (Six-base) MAPSORT of: KIEE5678.seq Check: 6331 from: 1 to: 1413 KIEE5678.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1365 1413 Size: 1365 48 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 874 1293 1413 Size: 874 419 120 Fragments arranged by size: 874 419 120 AhdI GACnn_n'nnGTC Cuts at: 0 672 1413 Size: 672 741 AlwNI CAG_nnn'CTG Cuts at: 0 81 582 1032 1413 Size: 81 501 450 381 Fragments arranged by size: 501 450 381 81 ApaI G_GGCC'C Cuts at: 0 288 1413 Size: 288 1125 ApoI r'AATT_y Cuts at: 0 152 1413 Size: 152 1261 AvrII C'CTAG_G Cuts at: 0 280 678 1413 Size: 280 398 735 Fragments arranged by size: 735 398 280 BanI G'GyrC_C Cuts at: 0 1099 1413 Size: 1099 314 BanII G_rGCy'C Cuts at: 0 288 1413 Size: 288 1125 BciVI GTATCCnnnnn_n' Cuts at: 0 703 1413 Size: 703 710 BglII A'GATC_T Cuts at: 0 815 1413 Size: 815 598 BlpI GC'TnA_GC Cuts at: 0 950 1413 Size: 950 463 Bme1580I G_kGCm'C Cuts at: 0 288 1413 Size: 288 1125 BmgBI CAC'GTC Cuts at: 0 1397 1413 Size: 1397 16 BmrI ACTGGGnnnn_n' Cuts at: 0 484 543 1413 Size: 484 59 870 Fragments arranged by size: 870 484 59 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 1235 1413 Size: 604 631 178 Fragments arranged by size: 631 604 178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 430 1413 Size: 116 314 983 Fragments arranged by size: 983 314 116 BsaI GGTCTCn'nnnn_ Cuts at: 0 434 1413 Size: 434 979 BsaHI Gr'CG_yC Cuts at: 0 640 1413 Size: 640 773 BsaWI w'CCGG_w Cuts at: 0 864 1413 Size: 864 549 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 22 883 1413 Size: 22 861 530 Fragments arranged by size: 861 530 22 BseYI C'CCAG_C Cuts at: 0 81 1413 Size: 81 1332 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 1039 1413 Size: 178 861 374 Fragments arranged by size: 861 374 178 BsmI GAATG_Cn' Cuts at: 0 1282 1413 Size: 1282 131 Bsp1286I G_dGCh'C Cuts at: 0 288 1413 Size: 288 1125 BspHI T'CATG_A Cuts at: 0 773 1413 Size: 773 640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1365 1413 Size: 1365 48 BsrDI GCAATG_nn' Cuts at: 0 428 1413 Size: 428 985 BsrFI r'CCGG_y Cuts at: 0 350 1413 Size: 350 1063 BstAPI GCAn_nnn'nTGC Cuts at: 0 976 1413 Size: 976 437 BstXI CCAn_nnnn'nTGG Cuts at: 0 1141 1413 Size: 1141 272 BstYI r'GATC_y Cuts at: 0 815 1029 1413 Size: 815 214 384 Fragments arranged by size: 815 384 214 BtsI GCAGTG_nn' Cuts at: 0 977 1413 Size: 977 436 DraIII CAC_nnn'GTG Cuts at: 0 1062 1413 Size: 1062 351 EaeI y'GGCC_r Cuts at: 0 827 1010 1413 Size: 827 183 403 Fragments arranged by size: 827 403 183 EarI CTCTTCn'nnn_ Cuts at: 0 797 1078 1379 1413 Size: 797 281 301 34 Fragments arranged by size: 797 301 281 34 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 874 1235 1293 1413 Size: 604 270 361 58 120 Fragments arranged by size: 604 361 270 120 58 EcoO109I rG'GnC_Cy Cuts at: 0 284 924 1413 Size: 284 640 489 Fragments arranged by size: 640 489 284 FspI TGC'GCA Cuts at: 0 434 1413 Size: 434 979 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 658 690 866 898 1413 Size: 658 32 176 32 515 Fragments arranged by size: 658 515 176 32 32 MfeI C'AATT_G Cuts at: 0 66 1413 Size: 66 1347 MscI TGG'CCA Cuts at: 0 829 1012 1413 Size: 829 183 401 Fragments arranged by size: 829 401 183 MslI CAynn'nnrTG Cuts at: 0 1133 1139 1413 Size: 1133 6 274 Fragments arranged by size: 1133 274 6 MspA1I CmG'CkG Cuts at: 0 954 1313 1413 Size: 954 359 100 Fragments arranged by size: 954 359 100 PflMI CCAn_nnn'nTGG Cuts at: 0 582 1413 Size: 582 831 PfoI T'CCnGG_A Cuts at: 0 1025 1413 Size: 1025 388 PspOMI G'GGCC_C Cuts at: 0 284 1413 Size: 284 1129 PstI C_TGCA'G Cuts at: 0 1304 1413 Size: 1304 109 PvuII CAG'CTG Cuts at: 0 954 1313 1413 Size: 954 359 100 Fragments arranged by size: 954 359 100 SapI GCTCTTCn'nnn_ Cuts at: 0 1379 1413 Size: 1379 34 SfcI C'TryA_G Cuts at: 0 1300 1413 Size: 1300 113 SmlI C'TyrA_G Cuts at: 0 131 445 1413 Size: 131 314 968 Fragments arranged by size: 968 314 131 StyI C'CwwG_G Cuts at: 0 280 528 678 1166 1413 Size: 280 248 150 488 247 Fragments arranged by size: 488 280 248 247 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 230 1413 Size: 230 1183 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1308 1340 1413 Size: 1308 32 73 Fragments arranged by size: 1308 73 32 Tth111I GACn'n_nGTC Cuts at: 0 1117 1413 Size: 1117 296 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 803 924 1164 1413 Size: 803 121 240 249 Fragments arranged by size: 803 249 240 121 Enzymes that do cut: AarI AcuI AhdI AlwNI ApaI ApoI AvrII BanI BanII BciVI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI BtsI DraIII EaeI EarI Eco57MI EcoO109I FspI Hin4I MfeI MscI MslI MspA1I PflMI PfoI PspOMI PstI PvuII SapI SfcI SmlI StyI TaqII TstI Tth111I XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI BglI BmtI BplI Bpu10I BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XhoI XmaI XmnI ZraI