################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HphI KasI KpnI KspI MaeII MamI MluI MluNI MroI MscI MslI MspA1I MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AvaII 239 GGACC G^G[AT]CC BbvI 4 GCTGC GCAGC(8/12) BcnI 236 CCCGG CC^[GC]GG BglII 547 AGATCT A^GATCT BsaI 500 GAGACC GGTCTC(1/5) BsmAI 500 GAGAC GTCTC(1/5) BsmFI 424 GTCCC GGGAC(10/14) BsrDI 43 GCAATG GCAATG(2/0) BsrI 13 CCAGT ACTGG(1/-1) BstF5I 531 GGATG GGATG(2/0) BstYI 547 AGATCT [GA]^GATC[CT] Cfr13I 239 GGACC G^G[ACGT]CC DpnII 548 GATC ^GATC DraI 508 TTTAAA TTT^AAA EarI 166 CTCTTC CTCTTC(1/4) EcoT14I 177 CCAAGG C^C[AT][AT]GG Fnu4HI 4 GCTGC GC^[ACGT]GC FokI 531 GGATG GGATG(9/13) HaeIII 188 GGCC GG^CC HapII 237 CCGG C^CGG HindIII 118 AAGCTT A^AGCTT HpaII 237 CCGG C^CGG ItaI 4 GCTGC GC^[ACGT]GC Ksp632I 166 CTCTTC CTCTTC(1/4) MboI 548 GATC ^GATC MfeI 24 CAATTG C^AATTG MflI 547 AGATCT [GA]^GATC[CT] MspI 237 CCGG C^CGG MunI 24 CAATTG C^AATTG NciI 236 CCCGG CC^[GC]GG NdeII 548 GATC ^GATC NlaIII 433 CATG CATG^ Sau3AI 548 GATC ^GATC Sau96I 239 GGACC G^G[ACGT]CC SfcI 219 CTGTAG C^T[GA][CT]AG StyI 177 CCAAGG C^C[AT][AT]GG TseI 4 GCTGC G^C[AT]GC Tsp45I 398 GTGAC ^GT[GC]AC TspRI 561 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 547 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BbsI 19 GAAGAC GAAGAC(2/6) 574 GAAGAC BciVI 387 GGATAC GTATCC(6/5) 472 GGATAC BfaI 278 CTAG C^TAG 481 CTAG BpuAI 19 GAAGAC GAAGAC(2/6) 574 GAAGAC BstNI 96 CCTGG CC^[AT]GG 427 CCAGG Cac8I 116 GCAAGC GC[ACGT]^[ACGT]GC 272 GCCAGC EcoRII 96 CCTGG ^CC[AT]GG 427 CCAGG MaeI 278 CTAG C^TAG 481 CTAG MaeIII 292 GTAAC ^GT[ACGT]AC 398 GTGAC MseI 356 TTAA T^TAA 509 TTAA MvaI 96 CCTGG CC^[AT]GG 427 CCAGG SfaNI 444 GATGC GCATC(5/9) 29 GCATC Tru9I 356 TTAA T^TAA 509 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AcsI AluI ApoI BsaJI BssKI HinfI MboII MnlI ScrFI TfiI Tsp509I