Restriction map for pFN21AE1677
 (Circular) (Six-base) MAPSORT of: pFN21AE1677.seq  Check: 5507  from: 1  to: 5284



pFN21AE1677  5284 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:52  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3094     278 
   Size:         53      83     186     936    1558    2468

  Fragments arranged by size: 

                2468    1558     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2627    1937 
   Size:        690    4594
 
Acc65I G'GTAC_C

Cuts at:   2605    2605 
   Size:       5284
 
AclI AA'CG_TT

Cuts at:   1563    3411    3784    1563 
   Size:       1848     373    3063

  Fragments arranged by size: 

                3063    1848     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2106    3347    4731    4999    1632 
   Size:        474    1241    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1241     474     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5087
 
AflIII A'CryG_T

Cuts at:   1203    4183    5160    1203 
   Size:       2980     977    1327

  Fragments arranged by size: 

                2980    1327     977
 
AgeI A'CCGG_T

Cuts at:   4115    4115 
   Size:       5284
 
AhdI GACnn_n'nnGTC

Cuts at:   4013    4013 
   Size:       5284
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5284
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5284
 
AlwNI CAG_nnn'CTG

Cuts at:   3027    4599    3027 
   Size:       1572    3712
 
ApaLI G'TGCA_C

Cuts at:   3341    4497    3341 
   Size:       1156    4128
 
ApoI r'AATT_y

Cuts at:   1493    2054    2152    2322    2593    2819    2971    5080    1493 
   Size:        561      98     170     271     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     561     271     226     170     152      98
            
 
AseI AT'TA_AT

Cuts at:    160    1032    3838     160 
   Size:        872    2806    1606

  Fragments arranged by size: 

                2806    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5284
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2609    1870 
   Size:         73     666    4545

  Fragments arranged by size: 

                4545     666      73
 
BamHI G'GATC_C

Cuts at:   2614    2981    2614 
   Size:        367    4917
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2605    4060     618 
   Size:        325     217     627     818    1455    1842

  Fragments arranged by size: 

                1842    1455     818     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2603    1391 
   Size:       1212    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2031    2586     928 
   Size:        971     132     555    3626

  Fragments arranged by size: 

                3626     971     555     132
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3475    3509    1903 
   Size:         34    1538      34    3678

  Fragments arranged by size: 

                3678    1538      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2386    2471    3176    4386    2386 
   Size:         85     705    1210    3284

  Fragments arranged by size: 

                3284    1210     705      85
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5284
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3895     136 
   Size:        107     122      71     631     605    2223    1525

  Fragments arranged by size: 

                2223    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2552    5279    2552 
   Size:       2727    2557
 
BlpI GC'TnA_GC

Cuts at:   2701    2701 
   Size:       5284
 
Bme1580I G_kGCm'C

Cuts at:   1790    3345    4501    1790 
   Size:       1555    1156    2573

  Fragments arranged by size: 

                2573    1555    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5284
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3015    3973     442 
   Size:        416      53      83     399      24    1598     958    1753

  Fragments arranged by size: 

                1753    1598     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5284
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3944    1239 
   Size:       2705    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3409    4274    4572    3409 
   Size:        865     298    4121

  Fragments arranged by size: 

                4121     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2499    3947     882 
   Size:        617    1000    1448    2219

  Fragments arranged by size: 

                2219    1448    1000     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3075     493 
   Size:        711    1871    2702

  Fragments arranged by size: 

                2702    1871     711
 
BsaBI GATnn'nnATC

Cuts at:   5278    5278 
   Size:       5284
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3091    3473    4108     275 
   Size:         53      83     186     936    1558     382     635    1451

  Fragments arranged by size: 

                1558    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3717    4115    4389    4536    4947    3717 
   Size:        398     274     147     411    4054

  Fragments arranged by size: 

                4054     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5059

  Fragments arranged by size: 

                5059     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5284
 
BseYI C'CCAG_C

Cuts at:   4487    4983    4487 
   Size:        496    4788
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4711
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2734    3495    4523    1540 
   Size:        275     186     733     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     733     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3345    3430    4501    3345 
   Size:         85    1071    4128

  Fragments arranged by size: 

                4128    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2795    2888    2795 
   Size:         93    5191
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2603    3345    3430    4501    1391 
   Size:        399     813     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     813     742     399      85
 
BspEI T'CCGG_A

Cuts at:   4947    4947 
   Size:       5284
 
BspHI T'CATG_A

Cuts at:   3173    4903    3173 
   Size:       1730    3554
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2639     844 
   Size:       1795    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3171    1394 
   Size:        429    1348    3507

  Fragments arranged by size: 

                3507    1348     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2055    3779    3953      65 
   Size:       1183     807    1724     174    1396

  Fragments arranged by size: 

                1724    1396    1183     807     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3928    4115    1700 
   Size:        110    2118     187    2869

  Fragments arranged by size: 

                2869    2118     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5284
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5284
 
BssSI C'ACGA_G

Cuts at:   3344    4356    3344 
   Size:       1012    4272
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5284
 
BstBI TT'CG_AA

Cuts at:   4088    4088 
   Size:       5284
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5284
 
BstYI r'GATC_y

Cuts at:   1972    2552    2614    2981    3366    3383    4835    4921    4933 
   Size:        580      62     367     385      17    1452      86      12

Cuts at:   4933    5279    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     580     385     367     346      86      62
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3793

  Fragments arranged by size: 

                3793     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4545

  Fragments arranged by size: 

                4545     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2800    3594    3614    2800 
   Size:        794      20    4470

  Fragments arranged by size: 

                4470     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5284
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5249
 
DraI TTT'AAA

Cuts at:   2515    2588    2515 
   Size:         73    5211
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4291     809 
   Size:        507    2975    1802

  Fragments arranged by size: 

                2975    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2731    3620    5249 
   Size:         54    1425      50     275      18     901     889    1629

Cuts at:   5249       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     901     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2731    1537 
   Size:        275     919    4090

  Fragments arranged by size: 

                4090     919     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2177    3214    1353 
   Size:        824    1037    3423

  Fragments arranged by size: 

                3423    1037     824
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3871    4245    4391     387 
   Size:       1064     138    2282     374     146    1280

  Fragments arranged by size: 

                2282    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2106    3347    3944    4731    4999    1239 
   Size:        393     474    1241     597     787     268    1524

  Fragments arranged by size: 

                1524    1241     787     597     474     393     268
 
EcoRI G'AATT_C

Cuts at:   2593    2971    2593 
   Size:        378    4906
 
FspI TGC'GCA

Cuts at:   3790    3790 
   Size:       5284
 
HaeII r_GCGC'y

Cuts at:   1678    4431    1678 
   Size:       2753    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3925    3957    3999    4031    1373 
   Size:         32     135      32    2353      32      42      32    2626

  Fragments arranged by size: 

                2626    2353     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2628    2874     669 
   Size:        988     281     690     246    3079

  Fragments arranged by size: 

                3079     988     690     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2123     748 
   Size:       1375    3909
 
HpaI GTT'AAC

Cuts at:   2874    2874 
   Size:       5284
 
KpnI G_GTAC'C

Cuts at:   2609    2609 
   Size:       5284
 
MfeI C'AATT_G

Cuts at:   2029    2883    2029 
   Size:        854    4430
 
MluI A'CGCG_T

Cuts at:   5160    5160 
   Size:       5284
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4398    4582    1305 
   Size:       3093     184    2007

  Fragments arranged by size: 

                3093    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3462

  Fragments arranged by size: 

                3462    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3242    3601    3760     518 
   Size:        648    2076     359     159    2042

  Fragments arranged by size: 

                2076    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2700    3377    4525    4770    1606 
   Size:       1094     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1094     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3793

  Fragments arranged by size: 

                3793     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5284
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5284
 
NotI GC'GGCC_GC

Cuts at:   2731    2731 
   Size:       5284
 
NspI r_CATG'y

Cuts at:   1132    1229    2642    4187    1132 
   Size:         97    1413    1545    2229

  Fragments arranged by size: 

                2229    1545    1413      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5284
 
PciI A'CATG_T

Cuts at:   4183    4183 
   Size:       5284
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5284
 
PmeI GTTT'AAAC

Cuts at:   2588    2588 
   Size:       5284
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3353    3385    4866    4898    3353 
   Size:         32    1481      32    3739

  Fragments arranged by size: 

                3739    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5284
 
PsiI TTA'TAA

Cuts at:   2258    2854    2258 
   Size:        596    4688
 
PstI C_TGCA'G

Cuts at:    830    2636     830 
   Size:       1806    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5284
 
SalI G'TCGA_C

Cuts at:   1936    2626    1936 
   Size:        690    4594
 
SbfI CC_TGCA'GG

Cuts at:   2636    2636 
   Size:       5284
 
ScaI AGT'ACT

Cuts at:   3532    5244    3532 
   Size:       1712    3572
 
SfcI C'TryA_G

Cuts at:    826    1045    2224    2632    3767    4448    4639     826 
   Size:        219    1179     408    1135     681     191    1471

  Fragments arranged by size: 

                1471    1179    1135     681     408     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5284
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5284
 
SmaI CCC'GGG

Cuts at:   1872    2611    1872 
   Size:        739    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3388    4289    4551     820 
   Size:        197     926    1445     901     262    1553

  Fragments arranged by size: 

                1553    1445     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3075     493 
   Size:       2582    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5284
 
SphI G_CATG'C

Cuts at:   1132    2642    1132 
   Size:       1510    3774
 
SspI AAT'ATT

Cuts at:      5      52    3208       5 
   Size:         47    3156    2081

  Fragments arranged by size: 

                3156    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5284
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2182    2723     513 
   Size:        552     939     178     541    3074

  Fragments arranged by size: 

                3074     939     552     541     178
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3311    3328    3481    3666    4111     325 
   Size:       1255    1731      17     153     185     445    1498

  Fragments arranged by size: 

                1731    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3530    5242      96 
   Size:        274      80      33      51    1443    1553    1712     138

  Fragments arranged by size: 

                1712    1553    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5244    5276    5244 
   Size:         32    5252
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5284
 
XbaI T'CTAG_A

Cuts at:   2620    2620 
   Size:       5284
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5284
 
XmaI C'CCGG_G

Cuts at:   1870    2609    1870 
   Size:        739    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3413    3413 
   Size:       5284
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3092     276 
   Size:         53      83     186     936    1558    2468

  Fragments arranged by size: 

                2468    1558     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com