################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmFI BsmI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AlwI 1023 GATCC GGATC(4/5) AlwNI 562 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 834 AGCGCT AGC^GCT Asp700I 1266 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 484 GTGCTC G[AT]GC[AT]^C BbsI 97 GTCTTC GAAGAC(2/6) BfaI 1219 CTAG C^TAG BpuAI 97 GTCTTC GAAGAC(2/6) BsaAI 337 TACGTG [CT]AC^GT[GA] BsiHKAI 484 GTGCTC G[AT]GC[AT]^C BsmAI 82 GAGAC GTCTC(1/5) BsmBI 82 GAGACG CGTCTC(1/5) BstAPI 1395 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1022 AGATCC [GA]^GATC[CT] DraI 1378 TTTAAA TTT^AAA DraIII 1296 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 211 CTCTTC CTCTTC(1/4) Eco47III 834 AGCGCT AGC^GCT EcoT14I 914 CCAAGG C^C[AT][AT]GG Ksp632I 211 CTCTTC CTCTTC(1/4) KspI 988 CCGCGG CCGC^GG MaeI 1219 CTAG C^TAG MflI 1022 AGATCC [GA]^GATC[CT] MseI 1379 TTAA T^TAA NaeI 1089 GCCGGC GCC^GGC NgoMIV 1089 GCCGGC G^CCGGC NheI 1218 GCTAGC G^CTAGC NspI 1097 GCATGC [GA]CATG^[CT] PvuII 184 CAGCTG CAG^CTG SacII 988 CCGCGG CCGC^GG SexAI 1148 ACCAGGT A^CC[AT]GGT SfiI 1110 GGCCAACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 733 CTCAAG C^T[CT][GA]AG SphI 1097 GCATGC GCATG^C StuI 447 AGGCCT AGG^CCT StyI 914 CCAAGG C^C[AT][AT]GG Tru9I 1379 TTAA T^TAA XcmI 1003 CCAGCCTCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1022 AGATCC [GA]^GATC[CT] XmnI 1266 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 851 CGCG CG^CG 989 CGCG ApaI 696 GGGCCC GGGCC^C 992 GGGCCC AviII 800 TGCGCA TGC^GCA 1154 TGCGCA BalI 2 TGGCCA TGG^CCA 659 TGGCCA BglI 1084 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1111 GCCAACGTGGC BpmI 1008 CTCCAG CTGGAG(16/14) 814 CTGGAG BseRI 793 GAGGAG GAGGAG(10/8) 817 GAGGAG Bsp1407I 272 TGTACA T^GTACA 641 TGTACA BspMI 614 ACCTGC ACCTGC(4/8) 1397 ACCTGC BsrFI 686 ACCGGC [GA]^CCGG[CT] 1089 GCCGGC BsrGI 272 TGTACA T^GTACA 641 TGTACA BstUI 851 CGCG CG^CG 989 CGCG BstXI 434 CCACCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 716 CCAAGACGCTGG Cfr10I 686 ACCGGC [GA]^CCGG[CT] 1089 GCCGGC DdeI 124 CTGAG C^T[ACGT]AG 891 CTCAG FspI 800 TGCGCA TGC^GCA 1154 TGCGCA HinfI 674 GAGTC G^A[ACGT]TC 883 GAGTC MluNI 2 TGGCCA TGG^CCA 659 TGGCCA MscI 2 TGGCCA TGG^CCA 659 TGGCCA MspA1I 184 CAGCTG C[AC]G^C[GT]G 988 CCGCGG MvnI 851 CGCG CG^CG 989 CGCG PleI 674 GAGTC GAGTC(4/5) 883 GAGTC PstI 187 CTGCAG CTGCA^G 982 CTGCAG SfcI 187 CTGCAG C^T[GA][CT]AG 982 CTGCAG SspBI 272 TGTACA T^GTACA 641 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcyI AfaI AluI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI BsoBI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaII HphI ItaI KasI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NarI NciI NdeII NlaIII NlaIV RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfoI SmaI TseI Tsp45I TspRI XmaCI XmaI