Restriction map for
pFN21AE9510
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmFI
BsmI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
SbfI ScaI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwI 1023 GATCC GGATC(4/5)
AlwNI 562 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 834 AGCGCT AGC^GCT
Asp700I 1266 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 484 GTGCTC G[AT]GC[AT]^C
BbsI 97 GTCTTC GAAGAC(2/6)
BfaI 1219 CTAG C^TAG
BpuAI 97 GTCTTC GAAGAC(2/6)
BsaAI 337 TACGTG [CT]AC^GT[GA]
BsiHKAI 484 GTGCTC G[AT]GC[AT]^C
BsmAI 82 GAGAC GTCTC(1/5)
BsmBI 82 GAGACG CGTCTC(1/5)
BstAPI 1395 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1022 AGATCC [GA]^GATC[CT]
DraI 1378 TTTAAA TTT^AAA
DraIII 1296 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 211 CTCTTC CTCTTC(1/4)
Eco47III 834 AGCGCT AGC^GCT
EcoT14I 914 CCAAGG C^C[AT][AT]GG
Ksp632I 211 CTCTTC CTCTTC(1/4)
KspI 988 CCGCGG CCGC^GG
MaeI 1219 CTAG C^TAG
MflI 1022 AGATCC [GA]^GATC[CT]
MseI 1379 TTAA T^TAA
NaeI 1089 GCCGGC GCC^GGC
NgoMIV 1089 GCCGGC G^CCGGC
NheI 1218 GCTAGC G^CTAGC
NspI 1097 GCATGC [GA]CATG^[CT]
PvuII 184 CAGCTG CAG^CTG
SacII 988 CCGCGG CCGC^GG
SexAI 1148 ACCAGGT A^CC[AT]GGT
SfiI 1110 GGCCAACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 733 CTCAAG C^T[CT][GA]AG
SphI 1097 GCATGC GCATG^C
StuI 447 AGGCCT AGG^CCT
StyI 914 CCAAGG C^C[AT][AT]GG
Tru9I 1379 TTAA T^TAA
XcmI 1003 CCAGCCTCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1022 AGATCC [GA]^GATC[CT]
XmnI 1266 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 851 CGCG CG^CG
989 CGCG
ApaI 696 GGGCCC GGGCC^C
992 GGGCCC
AviII 800 TGCGCA TGC^GCA
1154 TGCGCA
BalI 2 TGGCCA TGG^CCA
659 TGGCCA
BglI 1084 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1111 GCCAACGTGGC
BpmI 1008 CTCCAG CTGGAG(16/14)
814 CTGGAG
BseRI 793 GAGGAG GAGGAG(10/8)
817 GAGGAG
Bsp1407I 272 TGTACA T^GTACA
641 TGTACA
BspMI 614 ACCTGC ACCTGC(4/8)
1397 ACCTGC
BsrFI 686 ACCGGC [GA]^CCGG[CT]
1089 GCCGGC
BsrGI 272 TGTACA T^GTACA
641 TGTACA
BstUI 851 CGCG CG^CG
989 CGCG
BstXI 434 CCACCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
716 CCAAGACGCTGG
Cfr10I 686 ACCGGC [GA]^CCGG[CT]
1089 GCCGGC
DdeI 124 CTGAG C^T[ACGT]AG
891 CTCAG
FspI 800 TGCGCA TGC^GCA
1154 TGCGCA
HinfI 674 GAGTC G^A[ACGT]TC
883 GAGTC
MluNI 2 TGGCCA TGG^CCA
659 TGGCCA
MscI 2 TGGCCA TGG^CCA
659 TGGCCA
MspA1I 184 CAGCTG C[AC]G^C[GT]G
988 CCGCGG
MvnI 851 CGCG CG^CG
989 CGCG
PleI 674 GAGTC GAGTC(4/5)
883 GAGTC
PstI 187 CTGCAG CTGCA^G
982 CTGCAG
SfcI 187 CTGCAG C^T[GA][CT]AG
982 CTGCAG
SspBI 272 TGTACA T^GTACA
641 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BsiYI
BslI BsoBI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MnlI MslI
MspI MvaI MwoI NarI NciI
NdeII NlaIII NlaIV RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfoI
SmaI TseI Tsp45I TspRI XmaCI
XmaI