(Circular) (Six-base) MAPSORT of: pFN21AE9510.seq Check: 9483 from: 1 to: 6142 pFN21AE9510 6142 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3411 3411 Size: 6142 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 1937 Size: 1548 4594 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2203 4205 5589 5857 1632 Size: 571 2002 1384 268 1917 Fragments arranged by size: 2002 1917 1384 571 268 AfeI AGC'GCT Cuts at: 2841 2841 Size: 6142 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 5041 6018 1203 Size: 3838 977 1327 Fragments arranged by size: 3838 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3173 1184 Size: 1989 4153 AlwNI CAG_nnn'CTG Cuts at: 2572 3885 5457 2572 Size: 1313 1572 3257 Fragments arranged by size: 3257 1572 1313 ApaI G_GGCC'C Cuts at: 2705 3001 2705 Size: 296 5846 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 3451 3677 3829 5938 1493 Size: 1958 226 152 2109 1697 Fragments arranged by size: 2109 1958 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2035 2696 2780 2793 3183 3467 1870 Size: 73 92 661 84 13 390 284 4545 Fragments arranged by size: 4545 661 390 284 92 84 73 13 BamHI G'GATC_C Cuts at: 3472 3839 3472 Size: 367 5775 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2306 2497 2542 3399 Size: 325 217 627 462 57 191 45 857 Cuts at: 3399 3463 4918 618 Size: 64 1455 1842 Fragments arranged by size: 1842 1455 857 627 462 325 217 191 64 57 45 BanII G_rGCy'C Cuts at: 1391 2705 2747 3001 3461 1391 Size: 1314 42 254 460 4072 Fragments arranged by size: 4072 1314 460 254 42 BbeI G_GCGC'C Cuts at: 2253 2310 2501 2253 Size: 57 191 5894 Fragments arranged by size: 5894 191 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2095 928 Size: 971 196 4975 Fragments arranged by size: 4975 971 196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3095 3122 4753 Size: 107 122 71 631 605 1423 27 1631 Cuts at: 4753 136 Size: 1525 Fragments arranged by size: 1631 1525 1423 631 605 122 107 71 27 BglII A'GATC_T Cuts at: 6137 6137 Size: 6142 BlpI GC'TnA_GC Cuts at: 3559 3559 Size: 6142 Bme1580I G_kGCm'C Cuts at: 1790 2547 2705 3001 4203 5359 1790 Size: 757 158 296 1202 1156 2573 Fragments arranged by size: 2573 1202 1156 757 296 158 BmgBI CAC'GTC Cuts at: 1318 2064 1318 Size: 746 5396 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3873 4831 442 Size: 416 53 83 399 24 2456 958 1753 Fragments arranged by size: 2456 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3227 1056 Size: 2171 3971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2911 2911 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2840 2998 4802 1239 Size: 1601 158 1804 2579 Fragments arranged by size: 2579 1804 1601 158 Bpu10I CC'TnA_GC Cuts at: 2129 2896 2129 Size: 767 5375 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2723 4267 5132 5430 2723 Size: 1544 865 298 3435 Fragments arranged by size: 3435 1544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4805 882 Size: 617 3306 2219 Fragments arranged by size: 3306 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2344 3933 493 Size: 711 1140 1589 2702 Fragments arranged by size: 2702 1589 1140 711 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2250 2307 2498 3245 Size: 53 83 186 936 717 57 191 747 Cuts at: 3245 3949 4331 4966 275 Size: 704 382 635 1451 Fragments arranged by size: 1451 936 747 717 704 635 382 191 186 83 57 53 BsaWI w'CCGG_w Cuts at: 4575 4973 5247 5394 5805 4575 Size: 398 274 147 411 4912 Fragments arranged by size: 4912 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2996 3026 1178 Size: 30 165 30 1593 30 4294 Fragments arranged by size: 4294 1593 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2813 2837 1186 Size: 1627 24 4491 Fragments arranged by size: 4491 1627 24 BseYI C'CCAG_C Cuts at: 2581 2929 3007 5345 5841 2581 Size: 348 78 2338 496 2882 Fragments arranged by size: 2882 2338 496 348 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5569 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 4203 4288 5359 2493 Size: 1710 85 1071 3276 Fragments arranged by size: 3276 1710 1071 85 BsmI GAATG_Cn' Cuts at: 3653 3746 3653 Size: 93 6049 BsmBI CGTCTCn'nnnn_ Cuts at: 2081 2081 Size: 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2493 2547 2705 2747 3001 3461 4203 Size: 399 703 54 158 42 254 460 742 Cuts at: 4203 4288 5359 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 703 460 399 254 158 85 54 42 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 2364 2457 2634 4031 5761 2364 Size: 93 177 1397 1730 2745 Fragments arranged by size: 2745 1730 1397 177 93 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2628 3411 3497 844 Size: 1784 783 86 3489 Fragments arranged by size: 3489 1784 783 86 BsrBI CCG'CTC Cuts at: 1394 1823 4029 1394 Size: 429 2206 3507 Fragments arranged by size: 3507 2206 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2106 2604 2815 3061 4637 4811 65 Size: 1183 858 498 211 246 1576 174 1396 Fragments arranged by size: 1576 1396 1183 858 498 246 211 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2691 3094 4786 4973 1700 Size: 110 881 403 1692 187 2869 Fragments arranged by size: 2869 1692 881 403 187 110 BsrGI T'GTAC_A Cuts at: 96 2277 2646 96 Size: 2181 369 3592 Fragments arranged by size: 3592 2181 369 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 3406 794 Size: 2612 3530 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstXI CCAn_nnnn'nTGG Cuts at: 2446 2728 2446 Size: 282 5860 BstYI r'GATC_y Cuts at: 1972 3027 3472 3839 4224 4241 5693 5779 5791 Size: 1055 445 367 385 17 1452 86 12 Cuts at: 5791 6137 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1055 445 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2310 2862 2993 513 Size: 552 939 306 552 131 3662 Fragments arranged by size: 3662 939 552 552 306 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3127 487 Size: 683 56 1901 3502 Fragments arranged by size: 3502 1901 683 56 BtsI GCAGTG_nn' Cuts at: 2592 2908 3432 3658 4452 4472 2592 Size: 316 524 226 794 20 4262 Fragments arranged by size: 4262 794 524 316 226 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3385 3446 3385 Size: 61 6081 DraIII CAC_nnn'GTG Cuts at: 3306 3306 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2664 3114 Size: 54 1425 50 275 18 177 657 450 Cuts at: 3114 3589 4478 6107 8 Size: 475 889 1629 43 Fragments arranged by size: 1629 1425 889 657 475 450 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 4072 1353 Size: 869 1850 3423 Fragments arranged by size: 3423 1850 869 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4729 5103 5249 387 Size: 1064 138 3140 374 146 1280 Fragments arranged by size: 3140 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2203 2840 2998 4205 4802 5589 5857 Size: 393 571 637 158 1207 597 787 268 Cuts at: 5857 1239 Size: 1524 Fragments arranged by size: 1524 1207 787 637 597 571 393 268 158 EcoO109I rG'GnC_Cy Cuts at: 2701 2827 2998 2701 Size: 126 171 5845 Fragments arranged by size: 5845 171 126 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 FspI TGC'GCA Cuts at: 2807 3161 4648 2807 Size: 354 1487 4301 Fragments arranged by size: 4301 1487 354 HaeII r_GCGC'y Cuts at: 1678 2253 2310 2501 2843 5289 1678 Size: 575 57 191 342 2446 2531 Fragments arranged by size: 2531 2446 575 342 191 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4783 4815 4857 4889 1373 Size: 32 135 32 3211 32 42 32 2626 Fragments arranged by size: 3211 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2182 2203 3337 3486 3732 669 Size: 988 281 244 21 1134 149 246 3079 Fragments arranged by size: 3079 1134 988 281 246 244 149 21 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KasI G'GCGC_C Cuts at: 2249 2306 2497 2249 Size: 57 191 5894 Fragments arranged by size: 5894 191 57 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2601 5256 5440 1305 Size: 1296 2655 184 2007 Fragments arranged by size: 2655 2007 1296 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2666 10 Size: 54 1425 343 177 657 3486 Fragments arranged by size: 3486 1425 657 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 2444 2639 4100 4459 4618 518 Size: 648 993 285 195 1461 359 159 2042 Fragments arranged by size: 2042 1461 993 648 359 285 195 159 MspA1I CmG'CkG Cuts at: 1606 2191 2995 3558 4235 5383 5628 1606 Size: 585 804 563 677 1148 245 2120 Fragments arranged by size: 2120 1148 804 677 585 563 245 NaeI GCC'GGC Cuts at: 3096 3096 Size: 6142 NarI GG'CG_CC Cuts at: 2250 2307 2498 2250 Size: 57 191 5894 Fragments arranged by size: 5894 191 57 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4651 Fragments arranged by size: 4651 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NgoMIV G'CCGG_C Cuts at: 3094 3094 Size: 6142 NheI G'CTAG_C Cuts at: 1052 3223 1052 Size: 2171 3971 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 3106 3500 5045 1132 Size: 97 1877 394 1545 2229 Fragments arranged by size: 2229 1877 1545 394 97 PasI CC'CwG_GG Cuts at: 1348 2302 1348 Size: 954 5188 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6142 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PspOMI G'GGCC_C Cuts at: 2701 2997 2701 Size: 296 5846 PstI C_TGCA'G Cuts at: 830 2196 2991 3494 830 Size: 1366 795 503 3478 Fragments arranged by size: 3478 1366 795 503 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2191 2191 Size: 6142 SacII CC_GC'GG Cuts at: 2996 2996 Size: 6142 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SexAI A'CCwGG_T Cuts at: 3153 3153 Size: 6142 SfcI C'TryA_G Cuts at: 826 1045 2192 2987 3490 4625 5306 5497 826 Size: 219 1147 795 503 1135 681 191 1471 Fragments arranged by size: 1471 1147 1135 795 681 503 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3122 3122 Size: 6142 SfoI GGC'GCC Cuts at: 2251 2308 2499 2251 Size: 57 191 5894 Fragments arranged by size: 5894 191 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 2782 2795 3185 3469 1872 Size: 910 13 390 284 4545 Fragments arranged by size: 4545 910 390 284 13 SmlI C'TyrA_G Cuts at: 820 1017 1943 2738 4246 5147 5409 820 Size: 197 926 795 1508 901 262 1553 Fragments arranged by size: 1553 1508 926 901 795 262 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3106 3500 1132 Size: 1974 394 3774 Fragments arranged by size: 3774 1974 394 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 2454 1517 Size: 937 5205 StyI C'CwwG_G Cuts at: 513 1065 2004 2919 3581 513 Size: 552 939 915 662 3074 Fragments arranged by size: 3074 939 915 662 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2924 4169 4186 4339 4524 4969 325 Size: 1255 1344 1245 17 153 185 445 1498 Fragments arranged by size: 1498 1344 1255 1245 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 2646 4388 Size: 274 80 33 51 1443 300 369 1742 Cuts at: 4388 6100 96 Size: 1712 138 Fragments arranged by size: 1742 1712 1443 369 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6102 6134 6102 Size: 32 6110 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3015 3015 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 2780 2793 3183 3467 1870 Size: 910 13 390 284 4545 Fragments arranged by size: 4545 910 390 284 13 XmnI GAAnn'nnTTC Cuts at: 3275 4271 3275 Size: 996 5146 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SrfI SwaI