(Circular) (Six-base) MAPSORT of: pFN21AE3300.seq Check: 6361 from: 1 to: 12370 pFN21AE3300 12370 bp With 182 enzymes: SgfI * May 26, 2016 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2360 2534 2594 2708 3059 3209 3410 3776 3863 Size: 174 60 114 351 150 201 366 87 Cuts at: 3863 4088 4115 4148 4313 4346 4922 5003 5030 Size: 225 27 33 165 33 576 81 27 Cuts at: 5030 5150 5489 5603 5741 5774 5975 6125 8712 Size: 120 339 114 138 33 201 150 2587 Cuts at: 8712 8838 9145 2360 Size: 126 307 5585 Fragments arranged by size: 5585 2587 576 366 351 339 307 225 201 201 174 165 150 150 138 126 120 114 114 87 81 60 33 33 33 27 27 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2721 3363 7017 7626 Size: 53 83 186 936 1185 642 3654 609 Cuts at: 7626 8067 10180 278 Size: 441 2113 2468 Fragments arranged by size: 3654 2468 2113 1185 936 642 609 441 186 83 53 AccI GT'mk_AC Cuts at: 1937 4608 7032 9713 1937 Size: 2671 2424 2681 4594 Fragments arranged by size: 4594 2681 2671 2424 Acc65I G'GTAC_C Cuts at: 9691 9691 Size: 12370 AclI AA'CG_TT Cuts at: 1563 10497 10870 1563 Size: 8934 373 3063 Fragments arranged by size: 8934 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3914 4336 4912 6487 6607 7389 7415 8168 Size: 2282 422 576 1575 120 782 26 753 Cuts at: 8168 10433 11817 12085 1632 Size: 2265 1384 268 1917 Fragments arranged by size: 2282 2265 1917 1575 1384 782 753 576 422 268 120 26 AfeI AGC'GCT Cuts at: 5792 7119 5792 Size: 1327 11043 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 12173 AflIII A'CryG_T Cuts at: 1203 4329 4418 4478 4905 6035 6414 11269 12246 Size: 3126 89 60 427 1130 379 4855 977 Cuts at: 12246 1203 Size: 1327 Fragments arranged by size: 4855 3126 1327 1130 977 427 379 89 60 AgeI A'CCGG_T Cuts at: 11201 11201 Size: 12370 AhdI GACnn_n'nnGTC Cuts at: 5698 9562 11099 5698 Size: 3864 1537 6969 Fragments arranged by size: 6969 3864 1537 AleI CACnn'nnGTG Cuts at: 1166 2730 3659 4997 5190 5735 6725 6812 7097 Size: 1564 929 1338 193 545 990 87 285 Cuts at: 7097 1166 Size: 6439 Fragments arranged by size: 6439 1564 1338 990 929 545 285 193 87 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3122 3455 4472 7566 7913 1184 Size: 1938 333 1017 3094 347 5641 Fragments arranged by size: 5641 3094 1938 1017 347 333 AlwNI CAG_nnn'CTG Cuts at: 2470 3325 3498 3951 4515 5055 5268 5427 5463 Size: 855 173 453 564 540 213 159 36 Cuts at: 5463 6174 6484 7546 7942 9106 9193 9340 9448 Size: 711 310 1062 396 1164 87 147 108 Cuts at: 9448 10113 11685 2470 Size: 665 1572 3155 Fragments arranged by size: 3155 1572 1164 1062 855 711 665 564 540 453 396 310 213 173 159 147 108 87 36 ApaI G_GGCC'C Cuts at: 2168 2292 3224 3344 6134 6146 8884 9347 2168 Size: 124 932 120 2790 12 2738 463 5191 Fragments arranged by size: 5191 2790 2738 932 463 124 120 12 ApaLI G'TGCA_C Cuts at: 3458 3664 4333 4909 5137 5183 5651 9362 10427 Size: 206 669 576 228 46 468 3711 1065 Cuts at: 10427 11583 3458 Size: 1156 4245 Fragments arranged by size: 4245 3711 1156 1065 669 576 468 228 206 46 ApoI r'AATT_y Cuts at: 1493 6260 8887 9679 9905 10057 12166 1493 Size: 4767 2627 792 226 152 2109 1697 Fragments arranged by size: 4767 2627 2109 1697 792 226 152 AseI AT'TA_AT Cuts at: 160 1032 10924 160 Size: 872 9892 1606 Fragments arranged by size: 9892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12370 AvaI C'yCGr_G Cuts at: 1870 1943 2561 3208 3958 4118 6359 6701 8211 Size: 73 618 647 750 160 2241 342 1510 Cuts at: 8211 8762 9695 1870 Size: 551 933 4545 Fragments arranged by size: 4545 2241 1510 933 750 647 618 551 342 160 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6037 6070 6037 Size: 33 12337 BamHI G'GATC_C Cuts at: 4935 9700 10067 4935 Size: 4765 367 7238 Fragments arranged by size: 7238 4765 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2348 2582 2696 2813 Size: 325 217 627 233 328 234 114 117 Cuts at: 2813 3044 3260 3623 3851 3962 4076 4301 4472 Size: 231 216 363 228 111 114 225 171 Cuts at: 4472 4991 5105 5591 5729 5843 5907 5963 6029 Size: 519 114 486 138 114 64 56 66 Cuts at: 6029 7412 7754 8739 8846 8959 8995 9514 9691 Size: 1383 342 985 107 113 36 519 177 Cuts at: 9691 11146 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1383 985 627 519 519 486 363 342 328 325 234 233 231 228 225 217 216 177 171 138 117 114 114 114 114 113 111 107 66 64 56 36 BanII G_rGCy'C Cuts at: 1391 2168 2292 3158 3224 3308 3344 4634 4766 Size: 777 124 866 66 84 36 1290 132 Cuts at: 4766 4976 5367 5711 6134 6146 6219 6233 6273 Size: 210 391 344 423 12 73 14 40 Cuts at: 6273 6792 7362 7488 8386 8867 8884 9272 9347 Size: 519 570 126 898 481 17 388 75 Cuts at: 9347 9389 9689 1391 Size: 42 300 4072 Fragments arranged by size: 4072 1290 898 866 777 570 519 481 423 391 388 344 300 210 132 126 124 84 75 73 66 42 40 36 17 14 12 BbeI G_GCGC'C Cuts at: 2024 3048 5911 6033 7758 2024 Size: 1024 2863 122 1725 6636 Fragments arranged by size: 6636 2863 1725 1024 122 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 6811 7924 928 Size: 971 4912 1113 5374 Fragments arranged by size: 5374 4912 1113 971 BbvCI CC'TCA_GC Cuts at: 2195 2195 Size: 12370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4031 4065 5581 5615 5873 Size: 34 50 34 2010 34 1516 34 258 Cuts at: 5873 5907 10561 10595 1903 Size: 34 4654 34 3678 Fragments arranged by size: 4654 3678 2010 1516 258 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 10262 11472 10262 Size: 1210 11160 BclI T'GATC_A Cuts at: 1548 1548 Size: 12370 BfrBI ATG'CAT Cuts at: 3132 6093 7981 8320 3132 Size: 2961 1888 339 7182 Fragments arranged by size: 7182 2961 1888 339 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2103 2397 2580 Size: 107 122 71 631 605 431 294 183 Cuts at: 2580 2712 2745 2928 3558 3735 3783 3849 4122 Size: 132 33 183 630 177 48 66 273 Cuts at: 4122 4347 4581 4644 4695 4809 4923 5100 5151 Size: 225 234 63 51 114 114 177 51 Cuts at: 5151 5265 5409 5637 5775 6018 6081 6183 8018 Size: 114 144 228 138 243 63 102 1835 Cuts at: 8018 9291 10981 136 Size: 1273 1690 1525 Fragments arranged by size: 1835 1690 1525 1273 631 630 605 431 294 273 243 234 228 225 183 183 177 177 144 138 132 122 114 114 114 107 102 71 66 63 63 51 51 48 33 BglII A'GATC_T Cuts at: 12365 12365 Size: 12370 BlpI GC'TnA_GC Cuts at: 6791 9271 9787 6791 Size: 2480 516 9374 Fragments arranged by size: 9374 2480 516 Bme1580I G_kGCm'C Cuts at: 1790 2168 2275 2292 2351 2667 2699 3224 3344 Size: 378 107 17 59 316 32 525 120 Cuts at: 3344 3462 3554 3668 3854 3965 4079 4337 4418 Size: 118 92 114 186 111 114 258 81 Cuts at: 4418 4507 4823 4913 5141 5187 5261 5295 5594 Size: 89 316 90 228 46 74 34 299 Cuts at: 5594 5642 5655 5966 6014 6134 6146 6254 6636 Size: 48 13 311 48 120 12 108 382 Cuts at: 6636 7417 8399 8694 8742 8884 9347 9366 10431 Size: 781 982 295 48 142 463 19 1065 Cuts at: 10431 11587 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1065 982 781 525 463 382 378 316 316 311 299 295 258 228 186 142 120 120 118 114 114 111 108 107 92 90 89 81 74 59 48 48 48 46 34 32 19 17 13 12 BmgBI CAC'GTC Cuts at: 1318 3241 5290 7099 8641 9331 9402 1318 Size: 1923 2049 1809 1542 690 71 4286 Fragments arranged by size: 4286 2049 1923 1809 1542 690 71 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3576 5236 8113 Size: 416 53 83 399 24 2159 1660 2877 Cuts at: 8113 9350 9436 10101 11059 442 Size: 1237 86 665 958 1753 Fragments arranged by size: 2877 2159 1753 1660 1237 958 665 416 399 86 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12370 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2620 3391 4579 5098 5584 9628 2620 Size: 771 1188 519 486 4044 5362 Fragments arranged by size: 5362 4044 1188 771 519 486 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3811 7058 7478 7709 7868 8057 8633 8851 Size: 2572 3247 420 231 159 189 576 218 Cuts at: 8851 9598 11030 1239 Size: 747 1432 2579 Fragments arranged by size: 3247 2579 2572 1432 747 576 420 231 218 189 159 Bpu10I CC'TnA_GC Cuts at: 2195 9008 9519 2195 Size: 6813 511 5046 Fragments arranged by size: 6813 5046 511 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7370 8435 8516 10495 11360 11658 7370 Size: 1065 81 1979 865 298 8082 Fragments arranged by size: 8082 1979 1065 865 298 81 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2084 3800 4712 7169 7454 7784 8222 Size: 617 585 1716 912 2457 285 330 438 Cuts at: 8222 11033 882 Size: 2811 2219 Fragments arranged by size: 2811 2457 2219 1716 912 617 585 438 330 285 BsaAI yAC'GTr Cuts at: 493 1204 2230 2662 4330 4419 4479 4908 6036 Size: 711 1026 432 1668 89 60 429 1128 Cuts at: 6036 7219 10161 493 Size: 1183 2942 2702 Fragments arranged by size: 2942 2702 1668 1183 1128 1026 711 432 429 89 60 BsaBI GATnn'nnATC Cuts at: 7943 12364 7943 Size: 4421 7949 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 2718 3045 3360 Size: 53 83 186 936 488 697 327 315 Cuts at: 3360 3396 3753 5835 5908 6030 6930 7014 7608 Size: 36 357 2082 73 122 900 84 594 Cuts at: 7608 7623 7755 7994 8064 9452 9609 10177 10559 Size: 15 132 239 70 1388 157 568 382 Cuts at: 10559 11194 275 Size: 635 1451 Fragments arranged by size: 2082 1451 1388 936 900 697 635 594 568 488 382 357 327 315 239 186 157 132 122 84 83 73 70 53 36 15 BsaWI w'CCGG_w Cuts at: 7473 7947 9655 10803 11201 11475 11622 12033 7473 Size: 474 1708 1148 398 274 147 411 7810 Fragments arranged by size: 7810 1708 1148 474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2366 2396 3140 3170 3413 Size: 30 165 30 963 30 744 30 243 Cuts at: 3413 3443 3722 3752 3809 3839 4012 4042 4748 Size: 30 279 30 57 30 173 30 706 Cuts at: 4748 4778 5609 5639 6653 6683 9458 9488 1178 Size: 30 831 30 1014 30 2775 30 4060 Fragments arranged by size: 4060 2775 1014 963 831 744 706 279 243 173 165 57 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4847 5188 5564 6356 6887 6890 7060 7496 Size: 3661 341 376 792 531 3 170 436 Cuts at: 7496 7727 8201 9466 1186 Size: 231 474 1265 4090 Fragments arranged by size: 4090 3661 1265 792 531 474 436 376 341 231 170 3 BseYI C'CCAG_C Cuts at: 2079 2507 3256 3301 4508 5261 5294 5456 6423 Size: 428 749 45 1207 753 33 162 967 Cuts at: 6423 6915 6937 7129 8389 8486 8776 9110 9129 Size: 492 22 192 1260 97 290 334 19 Cuts at: 9129 9150 9347 9497 11573 12069 2079 Size: 21 197 150 2076 496 2380 Fragments arranged by size: 2380 2076 1260 1207 967 753 749 496 492 428 334 290 197 192 162 150 97 45 33 22 21 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3181 3488 4555 4753 4867 4948 5467 Size: 573 1445 307 1067 198 114 81 519 Cuts at: 5467 5746 5879 6457 6788 7082 7187 7190 7748 Size: 279 133 578 331 294 105 3 558 Cuts at: 7748 8084 8360 8483 9038 9164 9179 9353 1163 Size: 336 276 123 555 126 15 174 4180 Fragments arranged by size: 4180 1445 1067 578 573 558 555 519 336 331 307 294 279 276 198 174 133 126 123 114 105 81 15 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6717 6868 7230 7728 8791 9820 Size: 275 186 4716 151 362 498 1063 1029 Cuts at: 9820 10581 11609 1540 Size: 761 1028 2301 Fragments arranged by size: 4716 2301 1063 1029 1028 761 498 362 275 186 151 BsiHKAI G_wGCw'C Cuts at: 3254 3462 3668 3827 4337 4913 4976 5141 5187 Size: 208 206 159 510 576 63 165 46 Cuts at: 5187 5453 5627 5655 6792 8145 9014 9272 9366 Size: 266 174 28 1137 1353 869 258 94 Cuts at: 9366 9525 10431 10516 11587 3254 Size: 159 906 85 1071 4037 Fragments arranged by size: 4037 1353 1137 1071 906 869 576 510 266 258 208 206 174 165 159 159 94 85 63 46 28 BsmI GAATG_Cn' Cuts at: 6155 7106 9580 9651 9881 9974 6155 Size: 951 2474 71 230 93 8551 Fragments arranged by size: 8551 2474 951 230 93 71 BsmBI CGTCTCn'nnnn_ Cuts at: 3379 7685 8825 9595 9616 3379 Size: 4306 1140 770 21 6133 Fragments arranged by size: 6133 4306 1140 770 21 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2275 2292 2351 2667 2699 3158 Size: 399 378 107 17 59 316 32 459 Cuts at: 3158 3224 3254 3308 3344 3462 3554 3668 3827 Size: 66 30 54 36 118 92 114 159 Cuts at: 3827 3854 3965 4079 4337 4418 4507 4634 4766 Size: 27 111 114 258 81 89 127 132 Cuts at: 4766 4823 4913 4976 5141 5187 5261 5295 5367 Size: 57 90 63 165 46 74 34 72 Cuts at: 5367 5453 5594 5627 5642 5655 5711 5966 6014 Size: 86 141 33 15 13 56 255 48 Cuts at: 6014 6134 6146 6219 6233 6254 6273 6636 6792 Size: 120 12 73 14 21 19 363 156 Cuts at: 6792 7362 7417 7488 8145 8386 8399 8694 8742 Size: 570 55 71 657 241 13 295 48 Cuts at: 8742 8867 8884 9014 9272 9347 9366 9389 9525 Size: 125 17 130 258 75 19 23 136 Cuts at: 9525 9689 10431 10516 11587 1391 Size: 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 657 570 459 399 378 363 316 295 258 258 255 241 165 164 159 156 141 136 132 130 127 125 120 118 114 114 111 107 92 90 89 86 85 81 75 74 73 72 71 66 63 59 57 56 55 54 48 48 46 36 34 33 32 30 27 23 21 19 19 17 17 15 14 13 13 12 BspEI T'CCGG_A Cuts at: 7947 9655 12033 7947 Size: 1708 2378 8284 Fragments arranged by size: 8284 2378 1708 BspHI T'CATG_A Cuts at: 7659 10259 11989 7659 Size: 2600 1730 8040 Fragments arranged by size: 8040 2600 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2102 2360 2367 2534 2594 2708 2945 3059 Size: 1258 258 7 167 60 114 237 114 Cuts at: 3059 3209 3410 3776 3863 4088 4115 4148 4313 Size: 150 201 366 87 225 27 33 165 Cuts at: 4313 4346 4922 5003 5030 5150 5489 5603 5741 Size: 33 576 81 27 120 339 114 138 Cuts at: 5741 5774 5975 6065 6125 7569 8712 8838 9145 Size: 33 201 90 60 1444 1143 126 307 Cuts at: 9145 9201 9725 844 Size: 56 524 3489 Fragments arranged by size: 3489 1444 1258 1143 576 524 366 339 307 258 237 225 201 201 167 165 150 138 126 120 114 114 114 90 87 81 60 60 56 33 33 33 27 27 7 BsrBI CCG'CTC Cuts at: 1394 1823 2014 2653 7657 8419 8952 10257 1394 Size: 429 191 639 5004 762 533 1305 3507 Fragments arranged by size: 5004 3507 1305 762 639 533 429 191 BsrDI GCAATG_nn' Cuts at: 65 1248 10865 11039 65 Size: 1183 9617 174 1396 Fragments arranged by size: 9617 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2529 2753 4638 4694 4737 5273 5522 Size: 110 719 224 1885 56 43 536 249 Cuts at: 5522 6539 6716 7050 7195 7284 7524 7723 8790 Size: 1017 177 334 145 89 240 199 1067 Cuts at: 8790 11014 11201 1700 Size: 2224 187 2869 Fragments arranged by size: 2869 2224 1885 1067 1017 719 536 334 249 240 224 199 187 177 145 110 89 56 43 BsrGI T'GTAC_A Cuts at: 96 96 Size: 12370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12370 BssSI C'ACGA_G Cuts at: 2061 2726 10430 11442 2061 Size: 665 7704 1012 2989 Fragments arranged by size: 7704 2989 1012 665 BstAPI GCAn_nnn'nTGC Cuts at: 794 2205 2258 3498 3612 3951 4062 4176 4890 Size: 1411 53 1240 114 339 111 114 714 Cuts at: 4890 5055 5949 6174 6192 6484 7060 8568 9193 Size: 165 894 225 18 292 576 1508 625 Cuts at: 9193 9226 794 Size: 33 3938 Fragments arranged by size: 3938 1508 1411 1240 894 714 625 576 339 292 225 165 114 114 111 53 33 18 BstBI TT'CG_AA Cuts at: 11174 11174 Size: 12370 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 12370 BstXI CCAn_nnnn'nTGG Cuts at: 3406 3634 7099 7966 8708 9355 3406 Size: 228 3465 867 742 647 6421 Fragments arranged by size: 6421 3465 867 742 647 228 BstYI r'GATC_y Cuts at: 1972 4935 7938 9700 10067 10452 10469 11921 12007 Size: 2963 3003 1762 367 385 17 1452 86 Cuts at: 12007 12019 12365 1972 Size: 12 346 1977 Fragments arranged by size: 3003 2963 1977 1762 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 7314 7314 Size: 12370 BtgI C'CryG_G Cuts at: 513 1065 2515 2575 2709 2950 3040 3091 3555 Size: 552 1450 60 134 241 90 51 464 Cuts at: 3555 3844 4183 4578 4666 5530 5633 5839 5973 Size: 289 339 395 88 864 103 206 134 Cuts at: 5973 5992 7008 7018 7179 7377 7488 7575 7633 Size: 19 1016 10 161 198 111 87 58 Cuts at: 7633 8022 8601 8623 513 Size: 389 579 22 4260 Fragments arranged by size: 4260 1450 1016 864 579 552 464 395 389 339 289 241 206 198 161 134 134 111 103 90 88 87 60 58 51 22 19 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2086 2185 8287 487 Size: 683 56 860 99 6102 4570 Fragments arranged by size: 6102 4570 860 683 99 56 BtsI GCAGTG_nn' Cuts at: 2497 3460 3688 3799 4684 5392 5878 6407 6494 Size: 963 228 111 885 708 486 529 87 Cuts at: 6494 6634 7061 7082 7535 8654 9886 10680 10700 Size: 140 427 21 453 1119 1232 794 20 Cuts at: 10700 2497 Size: 4167 Fragments arranged by size: 4167 1232 1119 963 885 794 708 529 486 453 427 228 140 111 87 21 20 ClaI AT'CG_AT Cuts at: 1554 3705 3981 4041 1554 Size: 2151 276 60 9883 Fragments arranged by size: 9883 2151 276 60 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2530 2565 8912 8947 529 Size: 35 1966 35 6347 35 3952 Fragments arranged by size: 6347 3952 1966 35 35 35 DraI TTT'AAA Cuts at: 9674 9674 Size: 12370 DraIII CAC_nnn'GTG Cuts at: 2233 5536 7928 9142 2233 Size: 3303 2392 1214 5461 Fragments arranged by size: 5461 3303 2392 1214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7628 11377 809 Size: 507 6312 3749 1802 Fragments arranged by size: 6312 3749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2461 2551 Size: 54 1425 50 275 18 291 340 90 Cuts at: 2551 2755 3094 3511 5520 6162 6511 6714 6838 Size: 204 339 417 2009 642 349 203 124 Cuts at: 6838 6865 7101 7227 7297 7725 7806 8019 8205 Size: 27 236 126 70 428 81 213 186 Cuts at: 8205 8754 8788 9123 9283 9817 10706 12335 8 Size: 549 34 335 160 534 889 1629 43 Fragments arranged by size: 2009 1629 1425 889 642 549 534 428 417 349 340 339 335 291 275 236 213 204 203 186 160 126 124 90 81 70 54 50 43 34 27 18 EagI C'GGCC_G Cuts at: 1537 1812 6714 6865 7227 7725 8788 9817 1537 Size: 275 4902 151 362 498 1063 1029 4090 Fragments arranged by size: 4902 4090 1063 1029 498 362 275 151 EarI CTCTTCn'nnn_ Cuts at: 1353 6554 7704 10300 1353 Size: 5201 1150 2596 3423 Fragments arranged by size: 5201 3423 2596 1150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7006 8083 10957 11331 11477 387 Size: 1064 138 5417 1077 2874 374 146 1280 Fragments arranged by size: 5417 2874 1280 1077 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4974 6790 9270 4974 Size: 1816 2480 8074 Fragments arranged by size: 8074 2480 1816 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3811 3914 4336 4912 6487 6607 7058 Size: 393 2179 103 422 576 1575 120 451 Cuts at: 7058 7389 7415 7478 7709 7868 8057 8168 8633 Size: 331 26 63 231 159 189 111 465 Cuts at: 8633 8851 9598 10433 11030 11817 12085 1239 Size: 218 747 835 597 787 268 1524 Fragments arranged by size: 2179 1575 1524 835 787 747 597 576 465 451 422 393 331 268 231 218 189 159 120 111 103 63 26 EcoNI CCTnn'n_nnAGG Cuts at: 4148 7367 7847 4148 Size: 3219 480 8671 Fragments arranged by size: 8671 3219 480 EcoO109I rG'GnC_Cy Cuts at: 2154 2179 2289 2934 3341 3908 4188 5434 6131 Size: 25 110 645 407 567 280 1246 697 Cuts at: 6131 6143 6207 7453 8041 8527 8880 8881 9002 Size: 12 64 1246 588 486 353 1 121 Cuts at: 9002 9019 9103 9344 2154 Size: 17 84 241 5180 Fragments arranged by size: 5180 1246 1246 697 645 588 567 486 407 353 280 241 121 110 84 64 25 17 12 1 EcoRI G'AATT_C Cuts at: 9679 10057 9679 Size: 378 11992 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6637 6637 Size: 12370 FspI TGC'GCA Cuts at: 5319 6761 6884 7565 8363 10876 5319 Size: 1442 123 681 798 2513 6813 Fragments arranged by size: 6813 2513 1442 798 681 123 HaeII r_GCGC'y Cuts at: 1678 2024 2042 3030 3048 5794 5911 6033 7121 Size: 346 18 988 18 2746 117 122 1088 Cuts at: 7121 7285 7758 7843 8777 9078 11517 1678 Size: 164 473 85 934 301 2439 2531 Fragments arranged by size: 2746 2531 2439 1088 988 934 473 346 301 164 122 117 85 18 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3236 3268 3339 3371 4262 Size: 32 135 32 1664 32 71 32 891 Cuts at: 4262 4294 4376 4408 5435 5467 5684 5716 5804 Size: 32 82 32 1027 32 217 32 88 Cuts at: 5804 5836 6206 6238 8630 8662 8921 8953 9548 Size: 32 370 32 2392 32 259 32 595 Cuts at: 9548 9580 11011 11043 11085 11117 1373 Size: 32 1431 32 42 32 2626 Fragments arranged by size: 2626 2392 1664 1431 1027 891 595 370 259 217 135 88 82 71 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2542 2977 3364 3472 4294 4609 Size: 988 281 604 435 387 108 822 315 Cuts at: 4609 5335 5692 5809 7606 8068 9714 9960 669 Size: 726 357 117 1797 462 1646 246 3079 Fragments arranged by size: 3079 1797 1646 988 822 726 604 462 435 387 357 315 281 246 117 108 HindIII A'AGCT_T Cuts at: 748 748 Size: 12370 HpaI GTT'AAC Cuts at: 9960 9960 Size: 12370 KasI G'GCGC_C Cuts at: 2020 3044 5907 6029 7754 2020 Size: 1024 2863 122 1725 6636 Fragments arranged by size: 6636 2863 1725 1024 122 KpnI G_GTAC'C Cuts at: 9695 9695 Size: 12370 MfeI C'AATT_G Cuts at: 9969 9969 Size: 12370 MluI A'CGCG_T Cuts at: 6414 12246 6414 Size: 5832 6538 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2466 3183 4305 4419 6006 7761 9609 9618 Size: 1161 717 1122 114 1587 1755 1848 9 Cuts at: 9618 11484 11668 1305 Size: 1866 184 2007 Fragments arranged by size: 2007 1866 1848 1755 1587 1161 1122 717 184 114 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 2463 2553 7299 9125 10 Size: 54 1425 343 631 90 4746 1826 3255 Fragments arranged by size: 4746 3255 1826 1425 631 343 90 54 MslI CAynn'nnrTG Cuts at: 518 1166 2730 3060 3437 3659 4997 5190 5735 Size: 648 1564 330 377 222 1338 193 545 Cuts at: 5735 6725 6812 7097 7217 7548 9629 10328 10687 Size: 990 87 285 120 331 2081 699 359 Cuts at: 10687 10846 518 Size: 159 2042 Fragments arranged by size: 2081 2042 1564 1338 990 699 648 545 377 359 331 330 285 222 193 159 120 87 MspA1I CmG'CkG Cuts at: 1606 2268 2388 2613 2850 2905 3516 4113 4356 Size: 662 120 225 237 55 611 597 243 Cuts at: 4356 4680 4932 5250 5265 5400 5760 5913 5994 Size: 324 252 318 15 135 360 153 81 Cuts at: 5994 6334 6757 7020 7204 7556 7635 7677 7897 Size: 340 423 263 184 352 79 42 220 Cuts at: 7897 8374 8389 8874 9052 9282 9786 10463 11611 Size: 477 15 485 178 230 504 677 1148 Cuts at: 11611 11856 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 662 611 597 504 485 477 423 360 352 340 324 318 263 252 245 243 237 230 225 220 184 178 153 135 120 81 79 55 42 15 15 NaeI GCC'GGC Cuts at: 2531 4640 4696 4739 5524 6541 6718 7197 7286 Size: 2109 56 43 785 1017 177 479 89 Cuts at: 7286 7725 8792 2531 Size: 439 1067 6109 Fragments arranged by size: 6109 2109 1067 1017 785 479 439 177 89 56 43 NarI GG'CG_CC Cuts at: 2021 3045 5908 6030 7755 2021 Size: 1024 2863 122 1725 6636 Fragments arranged by size: 6636 2863 1725 1024 122 NcoI C'CATG_G Cuts at: 513 1065 2515 3091 7008 7575 8623 513 Size: 552 1450 576 3917 567 1048 4260 Fragments arranged by size: 4260 3917 1450 1048 576 567 552 NdeI CA'TA_TG Cuts at: 387 8280 387 Size: 7893 4477 NgoMIV G'CCGG_C Cuts at: 2529 4638 4694 4737 5522 6539 6716 7195 7284 Size: 2109 56 43 785 1017 177 479 89 Cuts at: 7284 7723 8790 2529 Size: 439 1067 6109 Fragments arranged by size: 6109 2109 1067 1017 785 479 439 177 89 56 43 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12370 NotI GC'GGCC_GC Cuts at: 7227 9817 7227 Size: 2590 9780 NsiI A_TGCA'T Cuts at: 3134 6095 7983 8322 3134 Size: 2961 1888 339 7182 Fragments arranged by size: 7182 2961 1888 339 NspI r_CATG'y Cuts at: 1132 1229 2226 3132 3281 3442 4027 6093 6698 Size: 97 997 906 149 161 585 2066 605 Cuts at: 6698 7570 7904 7981 8035 8320 8593 9634 9728 Size: 872 334 77 54 285 273 1041 94 Cuts at: 9728 11273 1132 Size: 1545 2229 Fragments arranged by size: 2229 2066 1545 1041 997 906 872 605 585 334 285 273 161 149 97 94 77 54 PasI CC'CwG_GG Cuts at: 1348 2275 4232 5149 7594 1348 Size: 927 1957 917 2445 6124 Fragments arranged by size: 6124 2445 1957 927 917 PciI A'CATG_T Cuts at: 11269 11269 Size: 12370 PflMI CCAn_nnn'nTGG Cuts at: 1108 2233 3241 4584 5268 6286 1108 Size: 1125 1008 1343 684 1018 7192 Fragments arranged by size: 7192 1343 1125 1018 1008 684 PfoI T'CCnGG_A Cuts at: 2788 5702 6285 7872 2788 Size: 2914 583 1587 7286 Fragments arranged by size: 7286 2914 1587 583 PmeI GTTT'AAAC Cuts at: 9674 9674 Size: 12370 PmlI CAC'GTG Cuts at: 2230 4419 4479 4908 6036 2230 Size: 2189 60 429 1128 8564 Fragments arranged by size: 8564 2189 1128 429 60 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10439 10471 11952 11984 10439 Size: 32 1481 32 10825 Fragments arranged by size: 10825 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2179 2934 3908 5434 6207 7453 8041 8527 2179 Size: 755 974 1526 773 1246 588 486 6022 Fragments arranged by size: 6022 1526 1246 974 773 755 588 486 PshAI GACnn'nnGTC Cuts at: 1613 2784 1613 Size: 1171 11199 PsiI TTA'TAA Cuts at: 9940 9940 Size: 12370 PspOMI G'GGCC_C Cuts at: 2164 2288 3220 3340 6130 6142 8880 9343 2164 Size: 124 932 120 2790 12 2738 463 5191 Fragments arranged by size: 5191 2790 2738 932 463 124 120 12 PstI C_TGCA'G Cuts at: 830 2267 2672 3002 3233 3330 4520 6395 6402 Size: 1437 405 330 231 97 1190 1875 7 Cuts at: 6402 6489 7676 8949 9033 9198 9231 9722 830 Size: 87 1187 1273 84 165 33 491 3478 Fragments arranged by size: 3478 1875 1437 1273 1190 1187 491 405 330 231 165 97 87 84 33 7 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12370 PvuII CAG'CTG Cuts at: 2268 2905 5760 6757 7204 8374 2268 Size: 637 2855 997 447 1170 6264 Fragments arranged by size: 6264 2855 1170 997 637 447 SacI G_AGCT'C Cuts at: 4976 6792 9272 4976 Size: 1816 2480 8074 Fragments arranged by size: 8074 2480 1816 SacII CC_GC'GG Cuts at: 5995 7021 7636 5995 Size: 1026 615 10729 Fragments arranged by size: 10729 1026 615 SalI G'TCGA_C Cuts at: 1936 4607 9712 1936 Size: 2671 5105 4594 Fragments arranged by size: 5105 4594 2671 SbfI CC_TGCA'GG Cuts at: 9722 9722 Size: 12370 ScaI AGT'ACT Cuts at: 2876 6500 6608 10618 12330 2876 Size: 3624 108 4010 1712 2916 Fragments arranged by size: 4010 3624 2916 1712 108 SexAI A'CCwGG_T Cuts at: 5436 5742 8355 9138 5436 Size: 306 2613 783 8668 Fragments arranged by size: 8668 2613 783 306 SfcI C'TryA_G Cuts at: 826 1045 2263 2668 2998 3229 3326 4516 4699 Size: 219 1218 405 330 231 97 1190 183 Cuts at: 4699 5131 5755 6292 6391 6398 6485 7672 8945 Size: 432 624 537 99 7 87 1187 1273 Cuts at: 8945 9029 9194 9227 9509 9718 10853 11534 11725 Size: 84 165 33 282 209 1135 681 191 Cuts at: 11725 826 Size: 1471 Fragments arranged by size: 1471 1273 1218 1190 1187 1135 681 624 537 432 405 330 282 231 219 209 191 183 165 99 97 87 84 33 7 SfiI GGCCn_nnn'nGGCC Cuts at: 9291 9291 Size: 12370 SfoI GGC'GCC Cuts at: 2022 3046 5909 6031 7756 2022 Size: 1024 2863 122 1725 6636 Fragments arranged by size: 6636 2863 1725 1024 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12370 SgrAI Cr'CCGG_yG Cuts at: 1700 7195 7284 1700 Size: 5495 89 6786 Fragments arranged by size: 6786 5495 89 SmaI CCC'GGG Cuts at: 1872 2563 8213 9697 1872 Size: 691 5650 1484 4545 Fragments arranged by size: 5650 4545 1484 691 SmlI C'TyrA_G Cuts at: 820 1017 1943 7385 8450 8531 10474 11375 11637 Size: 197 926 5442 1065 81 1943 901 262 Cuts at: 11637 820 Size: 1553 Fragments arranged by size: 5442 1943 1553 1065 926 901 262 197 81 SnaBI TAC'GTA Cuts at: 493 10161 493 Size: 9668 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 12370 SphI G_CATG'C Cuts at: 1132 3132 3281 3442 7904 7981 8035 8320 8593 Size: 2000 149 161 4462 77 54 285 273 Cuts at: 8593 9634 9728 1132 Size: 1041 94 3774 Fragments arranged by size: 4462 3774 2000 1041 285 273 161 149 94 77 54 SrfI GCCC'GGGC Cuts at: 2563 2563 Size: 12370 SspI AAT'ATT Cuts at: 5 52 10294 5 Size: 47 10242 2081 Fragments arranged by size: 10242 2081 47 StuI AGG'CCT Cuts at: 1517 2138 2483 6953 7067 8499 9662 1517 Size: 621 345 4470 114 1432 1163 4225 Fragments arranged by size: 4470 4225 1432 1163 621 345 114 StyI C'CwwG_G Cuts at: 513 1065 2515 3091 7008 7575 8235 8523 8623 Size: 552 1450 576 3917 567 660 288 100 Cuts at: 8623 9259 9809 513 Size: 636 550 3074 Fragments arranged by size: 3917 3074 1450 660 636 576 567 552 550 288 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2521 2645 2738 2963 3248 4224 4679 Size: 1255 941 124 93 225 285 976 455 Cuts at: 4679 4736 5255 5470 5774 5861 8738 8768 9143 Size: 57 519 215 304 87 2877 30 375 Cuts at: 9143 10397 10414 10567 10752 11197 325 Size: 1254 17 153 185 445 1498 Fragments arranged by size: 2877 1498 1255 1254 976 941 519 455 445 375 304 285 225 215 185 153 124 93 87 57 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 2874 6498 Size: 274 80 33 51 1443 399 498 3624 Cuts at: 6498 6606 8349 10616 12328 96 Size: 108 1743 2267 1712 138 Fragments arranged by size: 3624 2267 1743 1712 1443 498 399 274 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2366 2398 2496 2528 3413 3445 3722 3754 4237 Size: 32 98 32 885 32 277 32 483 Cuts at: 4237 4269 4634 4666 4927 4959 6569 6601 6653 Size: 32 365 32 261 32 1610 32 52 Cuts at: 6653 6685 9458 9490 12330 12362 2366 Size: 32 2773 32 2840 32 2374 Fragments arranged by size: 2840 2773 2374 1610 885 483 365 277 261 98 52 32 32 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3752 1316 Size: 2436 9934 XbaI T'CTAG_A Cuts at: 9706 9706 Size: 12370 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4483 5156 7033 7843 8242 8437 8764 8857 9118 Size: 673 1877 810 399 195 327 93 261 Cuts at: 9118 9286 4483 Size: 168 7567 Fragments arranged by size: 7567 1877 810 673 399 327 261 195 168 93 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 12370 XmaI C'CCGG_G Cuts at: 1870 2561 8211 9695 1870 Size: 691 5650 1484 4545 Fragments arranged by size: 5650 4545 1484 691 XmnI GAAnn'nnTTC Cuts at: 6500 10499 6500 Size: 3999 8371 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2719 3361 7015 7624 Size: 53 83 186 936 1185 642 3654 609 Cuts at: 7624 8065 10178 276 Size: 441 2113 2468 Fragments arranged by size: 3654 2468 2113 1185 936 642 609 441 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BsiWI BstZ17I EcoRV FseI FspAI NruI PacI PspXI PsrI RsrII SanDI SapI SwaI