Restriction map for
pFN21AE2356
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AflII AhdI Aor51HI AscI
AseI AsnI AspEI AvrII BciVI
BfrI BlnI BsiWI BspLU11I BssHII
Bst1107I BstBI BstZ17I CpoI DraI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoT22I FseI HindIII
HpaI Ksp632I KspI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SfiI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 4252 GACGTC GACGT^C
Acc65I 5098 GGTACC G^GTACC
AccI 934 GTCTAC GT^[AC][GT]AC
AccIII 7792 TCCGGA T^CCGGA
AcsI 3709 AAATTC [GA]^AATT[CT]
AgeI 7558 ACCGGT A^CCGGT
ApoI 3709 AAATTC [GA]^AATT[CT]
Asp718I 5098 GGTACC G^GTACC
AviII 6050 TGCGCA TGC^GCA
BclI 4994 TGATCA T^GATCA
BglII 4730 AGATCT A^GATCT
BlpI 228 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 228 GCTCAGC GC^T[ACGT]AGC
BsaBI 5505 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 7792 TCCGGA T^CCGGA
Bsp1407I 8072 TGTACA T^GTACA
BspDI 7432 ATCGAT AT^CGAT
BspEI 7792 TCCGGA T^CCGGA
BspHI 4608 TCATGA T^CATGA
BsrGI 8072 TGTACA T^GTACA
CelII 228 GCTCAGC GC^T[ACGT]AGC
ClaI 7432 ATCGAT AT^CGAT
EcoRV 3115 GATATC GAT^ATC
FbaI 4994 TGATCA T^GATCA
FspI 6050 TGCGCA TGC^GCA
KpnI 5098 GGTACC GGTAC^C
MamI 5505 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 7792 TCCGGA T^CCGGA
PinAI 7558 ACCGGT A^CCGGT
RcaI 4608 TCATGA T^CATGA
SbfI 4428 CCTGCAGG CCTGCA^GG
ScaI 1607 AGTACT AGT^ACT
Sse8387I 4428 CCTGCAGG CCTGCA^GG
SspBI 8072 TGTACA T^GTACA
Tsp509I 3710 AATT ^AATT
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 296 ACGTGT A^C[GA][CT]GT
5891 ACGTGT
AocI 709 CCTGAGG CC^T[ACGT]AGG
7666 CCTGAGG
Asp700I 117 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7959 GAACTCCTTC
AspI 2845 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7842 GACCAAGTC
BamHI 503 GGATCC G^GATCC
6694 GGATCC
BbsI 2325 GAAGAC GAAGAC(2/6)
5421 GAAGAC
BfaI 4153 CTAG C^TAG
7061 CTAG
BpuAI 2325 GAAGAC GAAGAC(2/6)
5421 GAAGAC
BsaWI 7558 ACCGGT [AT]^CCGG[AT]
7792 TCCGGA
BsiEI 3740 CGGTCG CG[GA][CT]^CG
6860 CGGTCG
Bsu36I 709 CCTGAGG CC^T[ACGT]AGG
7666 CCTGAGG
Eco81I 709 CCTGAGG CC^T[ACGT]AGG
7666 CCTGAGG
EcoNI 81 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3795 CCTGCGGGAGG
MaeI 4153 CTAG C^TAG
7061 CTAG
MseI 2207 TTAA T^TAA
2921 TTAA
PflFI 2845 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7842 GACCAAGTC
PshAI 2128 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4402 GACTGCAGTC
Tru9I 2207 TTAA T^TAA
2921 TTAA
Tth111I 2845 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7842 GACCAAGTC
XmnI 117 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7959 GAACTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BcnI BglI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PaeR7I PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI