################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AflIII Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 619 GACGTC GACGT^C AccIII 37 TCCGGA T^CCGGA AcyI 619 GACGTC G[GA]^CG[CT]C AfaI 492 GTAC GT^AC AgeI 888 ACCGGT A^CCGGT AhdI 597 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 517 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 597 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 581 TGGCCA TGG^CCA BcnI 95 CCCGG CC^[GC]GG BglI 4 GCCGACGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 619 GACGTC G[GA]^CG[CT]C BsaI 338 GGTCTC GGTCTC(1/5) BseAI 37 TCCGGA T^CCGGA BsiEI 886 CGACCG CG[GA][CT]^CG BsmFI 921 GTCCC GGGAC(10/14) BspDI 868 ATCGAT AT^CGAT BspEI 37 TCCGGA T^CCGGA BspMI 111 GCAGGT ACCTGC(4/8) BsrBI 559 GAGCGG CCGCTC(-3/-3) BstEII 369 GGTGACC G^GT[ACGT]ACC BstPI 369 GGTGACC G^GT[ACGT]ACC BstYI 905 AGATCC [GA]^GATC[CT] Cac8I 561 GCGGGC GC[ACGT]^[ACGT]GC CfoI 549 GCGC GCG^C ClaI 868 ATCGAT AT^CGAT DrdI 511 GACTACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 170 CCGTGG C^C[GA][CT]GG Eam1105I 597 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 369 GGTGACC G^GT[ACGT]ACC EcoRV 274 GATATC GAT^ATC EcoT14I 392 CCAAGG C^C[AT][AT]GG HgaI 7 GACGC GACGC(5/10) HhaI 549 GCGC GCG^C Hin1I 619 GACGTC G[GA]^CG[CT]C HinP1I 549 GCGC G^CGC MflI 905 AGATCC [GA]^GATC[CT] MluNI 581 TGGCCA TGG^CCA MroI 37 TCCGGA T^CCGGA MscI 581 TGGCCA TGG^CCA MspA1I 20 CAGCTG C[AC]G^C[GT]G NciI 95 CCCGG CC^[GC]GG PinAI 888 ACCGGT A^CCGGT PvuII 20 CAGCTG CAG^CTG RsaI 492 GTAC GT^AC SacI 123 GAGCTC GAGCT^C SbfI 108 CCTGCAGG CCTGCA^GG SexAI 515 ACCAGGT A^CC[AT]GGT Sse8387I 108 CCTGCAGG CCTGCA^GG StyI 392 CCAAGG C^C[AT][AT]GG TaqI 869 TCGA T^CGA Tsp509I 213 AATT ^AATT TthHB8I 869 TCGA T^CGA XhoII 905 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 9 CGCG CG^CG 550 CGCG AlwI 463 GATCC GGATC(4/5) 906 GATCC AspHI 123 GAGCTC G[AT]GC[AT]^C 241 GAGCAC AvaII 254 GGACC G^G[AT]CC 519 GGTCC BanII 123 GAGCTC G[GA]GC[CT]^C 499 GAGCCC BpmI 694 CTGGAG CTGGAG(16/14) 788 CTGGAG BsaBI 79 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 348 GATGGTCATC BsiHKAI 123 GAGCTC G[AT]GC[AT]^C 241 GAGCAC BsrFI 28 GCCGGT [GA]^CCGG[CT] 888 ACCGGT BsrI 693 ACTGG ACTGG(1/-1) 382 CCAGT BstNI 251 CCAGG CC^[AT]GG 516 CCAGG BstUI 9 CGCG CG^CG 550 CGCG Cfr10I 28 GCCGGT [GA]^CCGG[CT] 888 ACCGGT EcoRII 251 CCAGG ^CC[AT]GG 516 CCAGG MaeII 490 ACGT A^CGT 620 ACGT MamI 79 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 348 GATGGTCATC MvaI 251 CCAGG CC^[AT]GG 516 CCAGG MvnI 9 CGCG CG^CG 550 CGCG NlaIII 579 CATG CATG^ 777 CATG PleI 597 GACTC GAGTC(4/5) 603 GAGTC PpuMI 253 AGGACCC [GA]G^G[AT]CC[CT] 518 AGGTCCT SapI 676 GCTCTTC GCTCTTC(1/4) 763 GCTCTTC TfiI 721 GATTC G^A[AT]TC 784 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsaWI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MwoI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI