(Circular) (Six-base) MAPSORT of: pFN21AE3410.seq Check: 891 from: 1 to: 5635 pFN21AE3410 5635 bp With 182 enzymes: SgfI * March 28, 2012 12:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2107 2107 Size: 5635 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2628 3445 278 Size: 53 83 186 936 1092 817 2468 Fragments arranged by size: 2468 1092 936 817 186 83 53 AccI GT'mk_AC Cuts at: 1937 2978 1937 Size: 1041 4594 Acc65I G'GTAC_C Cuts at: 2956 2956 Size: 5635 AclI AA'CG_TT Cuts at: 1563 3762 4135 1563 Size: 2199 373 3063 Fragments arranged by size: 3063 2199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3698 5082 5350 1632 Size: 2066 1384 268 1917 Fragments arranged by size: 2066 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5438 AflIII A'CryG_T Cuts at: 1203 4534 5511 1203 Size: 3331 977 1327 Fragments arranged by size: 3331 1327 977 AgeI A'CCGG_T Cuts at: 2893 4466 2893 Size: 1573 4062 AhdI GACnn_n'nnGTC Cuts at: 2607 4364 2607 Size: 1757 3878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5635 AlwNI CAG_nnn'CTG Cuts at: 2527 3378 4950 2527 Size: 851 1572 3212 Fragments arranged by size: 3212 1572 851 ApaLI G'TGCA_C Cuts at: 3692 4848 3692 Size: 1156 4479 ApoI r'AATT_y Cuts at: 1493 2944 3170 3322 5431 1493 Size: 1451 226 152 2109 1697 Fragments arranged by size: 2109 1697 1451 226 152 AseI AT'TA_AT Cuts at: 160 1032 4189 160 Size: 872 3157 1606 Fragments arranged by size: 3157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2960 1870 Size: 73 1017 4545 Fragments arranged by size: 4545 1017 73 BamHI G'GATC_C Cuts at: 2965 3332 2965 Size: 367 5268 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2956 4411 618 Size: 325 217 627 1169 1455 1842 Fragments arranged by size: 1842 1455 1169 627 325 217 BanII G_rGCy'C Cuts at: 1391 2132 2508 2954 1391 Size: 741 376 446 4072 Fragments arranged by size: 4072 741 446 376 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4664 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3826 3860 1903 Size: 34 1889 34 3678 Fragments arranged by size: 3678 1889 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3527 4737 3527 Size: 1210 4425 BclI T'GATC_A Cuts at: 1548 1548 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 4246 136 Size: 107 122 71 631 605 343 2231 1525 Fragments arranged by size: 2231 1525 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 5630 5630 Size: 5635 BlpI GC'TnA_GC Cuts at: 3052 3052 Size: 5635 Bme1580I G_kGCm'C Cuts at: 1790 2572 3696 4852 1790 Size: 782 1124 1156 2573 Fragments arranged by size: 2573 1156 1124 782 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5635 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3366 4324 442 Size: 416 53 83 399 24 1949 958 1753 Fragments arranged by size: 1949 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2830 2830 Size: 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2720 2814 4295 1239 Size: 1481 94 1481 2579 Fragments arranged by size: 2579 1481 1481 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3760 4625 4923 3760 Size: 865 298 4472 Fragments arranged by size: 4472 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2349 4298 882 Size: 617 850 1949 2219 Fragments arranged by size: 2219 1949 850 617 BsaAI yAC'GTr Cuts at: 493 1204 3426 493 Size: 711 2222 2702 Fragments arranged by size: 2702 2222 711 BsaBI GATnn'nnATC Cuts at: 2088 2357 5629 2088 Size: 269 3272 2094 Fragments arranged by size: 3272 2094 269 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2625 3442 3824 4459 Size: 53 83 186 936 1092 817 382 635 Cuts at: 4459 275 Size: 1451 Fragments arranged by size: 1451 1092 936 817 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2042 2371 2893 4068 4466 4740 4887 5298 2042 Size: 329 522 1175 398 274 147 411 2379 Fragments arranged by size: 2379 1175 522 411 398 329 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2353 2383 1178 Size: 30 165 30 950 30 4430 Fragments arranged by size: 4430 950 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2119 2358 2876 1186 Size: 933 239 518 3945 Fragments arranged by size: 3945 933 518 239 BseYI C'CCAG_C Cuts at: 2027 2262 2444 4838 5334 2027 Size: 235 182 2394 496 2328 Fragments arranged by size: 2394 2328 496 235 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5062 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2894 3085 3846 4874 1540 Size: 275 186 893 191 761 1028 2301 Fragments arranged by size: 2301 1028 893 761 275 191 186 BsiHKAI G_wGCw'C Cuts at: 2132 2250 3696 3781 4852 2132 Size: 118 1446 85 1071 2915 Fragments arranged by size: 2915 1446 1071 118 85 BsmI GAATG_Cn' Cuts at: 3146 3239 3146 Size: 93 5542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2132 2250 2508 2572 2954 3696 3781 Size: 399 342 118 258 64 382 742 85 Cuts at: 3781 4852 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 342 258 118 85 64 BspEI T'CCGG_A Cuts at: 2042 5298 2042 Size: 3256 2379 BspHI T'CATG_A Cuts at: 3524 5254 3524 Size: 1730 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2107 2990 844 Size: 1263 883 3489 Fragments arranged by size: 3489 1263 883 BsrBI CCG'CTC Cuts at: 1394 1823 2566 3522 1394 Size: 429 743 956 3507 Fragments arranged by size: 3507 956 743 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4130 4304 65 Size: 1183 2882 174 1396 Fragments arranged by size: 2882 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 2893 4279 4466 1700 Size: 110 223 860 1386 187 2869 Fragments arranged by size: 2869 1386 860 223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5635 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5635 BssSI C'ACGA_G Cuts at: 3695 4707 3695 Size: 1012 4623 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5635 BstBI TT'CG_AA Cuts at: 4439 4439 Size: 5635 BstEII G'GTnAC_C Cuts at: 1184 2374 1184 Size: 1190 4445 BstYI r'GATC_y Cuts at: 1972 2910 2965 3332 3717 3734 5186 5272 5284 Size: 938 55 367 385 17 1452 86 12 Cuts at: 5284 5630 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 938 385 367 346 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2175 513 Size: 552 939 171 3973 Fragments arranged by size: 3973 939 552 171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2571 487 Size: 683 56 1345 3551 Fragments arranged by size: 3551 1345 683 56 BtsI GCAGTG_nn' Cuts at: 2361 2406 3151 3945 3965 2361 Size: 45 745 794 20 4031 Fragments arranged by size: 4031 794 745 45 20 ClaI AT'CG_AT Cuts at: 1554 2874 1554 Size: 1320 4315 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5600 DraI TTT'AAA Cuts at: 2939 2939 Size: 5635 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2522 4642 809 Size: 507 1206 2120 1802 Fragments arranged by size: 2120 1802 1206 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2019 2586 Size: 54 1425 50 275 18 177 12 567 Cuts at: 2586 3082 3971 5600 8 Size: 496 889 1629 43 Fragments arranged by size: 1629 1425 889 567 496 275 177 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3082 1537 Size: 275 1270 4090 Fragments arranged by size: 4090 1270 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 2712 2775 3565 1353 Size: 1335 24 63 790 3423 Fragments arranged by size: 3423 1335 790 63 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4222 4596 4742 387 Size: 1064 138 2633 374 146 1280 Fragments arranged by size: 2633 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2130 2130 Size: 5635 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2720 2814 3698 4295 5082 5350 1239 Size: 393 1088 94 884 597 787 268 1524 Fragments arranged by size: 1524 1088 884 787 597 393 268 94 EcoO109I rG'GnC_Cy Cuts at: 2259 2401 2524 2259 Size: 142 123 5370 Fragments arranged by size: 5370 142 123 EcoRI G'AATT_C Cuts at: 2944 3322 2944 Size: 378 5257 EcoRV GAT'ATC Cuts at: 2281 2281 Size: 5635 FspI TGC'GCA Cuts at: 4141 4141 Size: 5635 HaeII r_GCGC'y Cuts at: 1678 4782 1678 Size: 3104 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 2495 2527 2593 Size: 32 135 32 584 32 307 32 66 Cuts at: 2593 2625 4276 4308 4350 4382 1373 Size: 32 1651 32 42 32 2626 Fragments arranged by size: 2626 1651 584 307 135 66 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2979 3225 669 Size: 988 281 1041 246 3079 Fragments arranged by size: 3079 1041 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5635 HpaI GTT'AAC Cuts at: 3225 3225 Size: 5635 KpnI G_GTAC'C Cuts at: 2960 2960 Size: 5635 MfeI C'AATT_G Cuts at: 3234 3234 Size: 5635 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2516 4749 4933 1305 Size: 1211 2233 184 2007 Fragments arranged by size: 2233 2007 1211 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2588 10 Size: 54 1425 343 756 3057 Fragments arranged by size: 3057 1425 756 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3593 3952 4111 518 Size: 648 2427 359 159 2042 Fragments arranged by size: 2427 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 3051 3728 4876 5121 1606 Size: 421 1024 677 1148 245 2120 Fragments arranged by size: 2120 1148 1024 677 421 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4144 Fragments arranged by size: 4144 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5635 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 3082 3082 Size: 5635 NspI r_CATG'y Cuts at: 1132 1229 2993 4538 1132 Size: 97 1764 1545 2229 Fragments arranged by size: 2229 1764 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5635 PciI A'CATG_T Cuts at: 4534 4534 Size: 5635 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5635 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2259 2524 2259 Size: 265 5370 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5635 PsiI TTA'TAA Cuts at: 3205 3205 Size: 5635 PstI C_TGCA'G Cuts at: 830 2118 2202 2413 2826 2987 830 Size: 1288 84 211 413 161 3478 Fragments arranged by size: 3478 1288 413 211 161 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5635 PvuII CAG'CTG Cuts at: 2027 2027 Size: 5635 SacI G_AGCT'C Cuts at: 2132 2132 Size: 5635 SalI G'TCGA_C Cuts at: 1936 2977 1936 Size: 1041 4594 SapI GCTCTTCn'nnn_ Cuts at: 2688 2775 2688 Size: 87 5548 SbfI CC_TGCA'GG Cuts at: 2118 2987 2118 Size: 869 4766 ScaI AGT'ACT Cuts at: 3883 5595 3883 Size: 1712 3923 SexAI A'CCwGG_T Cuts at: 2520 2520 Size: 5635 SfcI C'TryA_G Cuts at: 826 1045 2114 2198 2409 2822 2983 4118 4799 Size: 219 1069 84 211 413 161 1135 681 Cuts at: 4799 4990 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1069 681 413 219 211 191 161 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2962 1872 Size: 1090 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3739 4640 4902 820 Size: 197 926 1796 901 262 1553 Fragments arranged by size: 1796 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2993 1132 Size: 1861 3774 SspI AAT'ATT Cuts at: 5 52 3559 5 Size: 47 3507 2081 Fragments arranged by size: 3507 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5635 StyI C'CwwG_G Cuts at: 513 1065 2004 2397 3074 513 Size: 552 939 393 677 3074 Fragments arranged by size: 3074 939 677 552 393 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3662 3679 3832 4017 4462 325 Size: 1255 2082 17 153 185 445 1498 Fragments arranged by size: 2082 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3881 5593 96 Size: 274 80 33 51 1443 1904 1712 138 Fragments arranged by size: 1904 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2353 2385 5595 5627 2353 Size: 32 3210 32 2361 Fragments arranged by size: 3210 2361 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5635 XbaI T'CTAG_A Cuts at: 2971 2971 Size: 5635 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5635 XmaI C'CCGG_G Cuts at: 1870 2960 1870 Size: 1090 4545 XmnI GAAnn'nnTTC Cuts at: 3764 3764 Size: 5635 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2626 3443 276 Size: 53 83 186 936 1092 817 2468 Fragments arranged by size: 2468 1092 936 817 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI