Restriction map for pFN21AE9504
 (Linear) (Six-base) MAPSORT of: KIEE9504.seq  Check: 7260  from: 1  to: 1164



KIEE9504.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:40  ..

AccI GT'mk_AC

Cuts at:      0     374     638    1164 
   Size:        374     264     526

  Fragments arranged by size: 

                 526     374     264
 
AfeI AGC'GCT

Cuts at:      0      81    1164 
   Size:         81    1083
 
AflIII A'CryG_T

Cuts at:      0     386     881    1164 
   Size:        386     495     283

  Fragments arranged by size: 

                 495     386     283
 
AlwNI CAG_nnn'CTG

Cuts at:      0     840    1164 
   Size:        840     324
 
ApaI G_GGCC'C

Cuts at:      0     116    1164 
   Size:        116    1048
 
ApaLI G'TGCA_C

Cuts at:      0     445    1164 
   Size:        445     719
 
AvaI C'yCGr_G

Cuts at:      0      25     115    1164 
   Size:         25      90    1049

  Fragments arranged by size: 

                1049      90      25
 
BanII G_rGCy'C

Cuts at:      0     116     284    1028    1164 
   Size:        116     168     744     136

  Fragments arranged by size: 

                 744     168     136     116
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     241     393     621    1164 
   Size:        241     152     228     543

  Fragments arranged by size: 

                 543     241     228     152
 
BbvCI CC'TCA_GC

Cuts at:      0     895    1016    1164 
   Size:        895     121     148

  Fragments arranged by size: 

                 895     148     121
 
BglI GCCn_nnn'nGGC

Cuts at:      0     316     622    1164 
   Size:        316     306     542

  Fragments arranged by size: 

                 542     316     306
 
BlpI GC'TnA_GC

Cuts at:      0     292     367     418    1164 
   Size:        292      75      51     746

  Fragments arranged by size: 

                 746     292      75      51
 
Bme1580I G_kGCm'C

Cuts at:      0     116     302     449    1084    1164 
   Size:        116     186     147     635      80

  Fragments arranged by size: 

                 635     186     147     116      80
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1028    1164 
   Size:       1028     136
 
Bpu10I CC'TnA_GC

Cuts at:      0     895    1016    1164 
   Size:        895     121     148

  Fragments arranged by size: 

                 895     148     121
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1036    1164 
   Size:       1036     128
 
BsaAI yAC'GTr

Cuts at:      0     435     828     882    1164 
   Size:        435     393      54     282

  Fragments arranged by size: 

                 435     393     282      54
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112    1033    1036    1164 
   Size:        112     921       3     128

  Fragments arranged by size: 

                 921     128     112       3
 
BseYI C'CCAG_C

Cuts at:      0      42     478     651    1164 
   Size:         42     436     173     513

  Fragments arranged by size: 

                 513     436     173      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     853    1164 
   Size:        853     311
 
BsiEI CG_ry'CG

Cuts at:      0     320    1128    1164 
   Size:        320     808      36

  Fragments arranged by size: 

                 808     320      36
 
BsiHKAI G_wGCw'C

Cuts at:      0     284     368     419     449     809    1164 
   Size:        284      84      51      30     360     355

  Fragments arranged by size: 

                 360     355     284      84      51      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     284     302     368     419     449     809    1028 
   Size:        116     168      18      66      51      30     360     219

Cuts at:   1028    1084    1164 
   Size:         56      80

  Fragments arranged by size: 

                 360     219     168     116      80      66      56      51
                  30      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     263     605     701    1164 
   Size:        263     342      96     463

  Fragments arranged by size: 

                 463     342     263      96
 
BsrFI r'CCGG_y

Cuts at:      0     470     564    1164 
   Size:        470      94     600

  Fragments arranged by size: 

                 600     470      94
 
BssHII G'CGCG_C

Cuts at:      0     456     714     729    1164 
   Size:        456     258      15     435

  Fragments arranged by size: 

                 456     435     258      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      70     453    1164 
   Size:         70     383     711

  Fragments arranged by size: 

                 711     383      70
 
BtgI C'CryG_G

Cuts at:      0     440     584     719    1164 
   Size:        440     144     135     445

  Fragments arranged by size: 

                 445     440     144     135
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     525    1164 
   Size:        525     639
 
BtsI GCAGTG_nn'

Cuts at:      0     607    1043    1164 
   Size:        607     436     121

  Fragments arranged by size: 

                 607     436     121
 
EaeI y'GGCC_r

Cuts at:      0     266     317     437     485     576     604     674    1125 
   Size:        266      51     120      48      91      28      70     451

Cuts at:   1125    1164 
   Size:         39

  Fragments arranged by size: 

                 451     266     120      91      70      51      48      39
                  28
 
EagI C'GGCC_G

Cuts at:      0     317    1125    1164 
   Size:        317     808      39

  Fragments arranged by size: 

                 808     317      39
 
EarI CTCTTCn'nnn_

Cuts at:      0     289    1164 
   Size:        289     875
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     103     109     943    1164 
   Size:        103       6     834     221

  Fragments arranged by size: 

                 834     221     103       6
 
EcoICRI GAG'CTC

Cuts at:      0     282    1164 
   Size:        282     882
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1028    1164 
   Size:       1028     136
 
EcoO109I rG'GnC_Cy

Cuts at:      0     112    1164 
   Size:        112    1052
 
HaeII r_GCGC'y

Cuts at:      0       8      83     756    1164 
   Size:          8      75     673     408

  Fragments arranged by size: 

                 673     408      75       8
 
HincII GTy'rAC

Cuts at:      0     375     876    1164 
   Size:        375     501     288

  Fragments arranged by size: 

                 501     375     288
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     968    1164 
   Size:        968     196
 
MscI TGG'CCA

Cuts at:      0     487     606     676    1164 
   Size:        487     119      70     488

  Fragments arranged by size: 

                 488     487     119      70
 
MslI CAynn'nnrTG

Cuts at:      0     208    1164 
   Size:        208     956
 
MspA1I CmG'CkG

Cuts at:      0     837     911     969    1164 
   Size:        837      74      58     195

  Fragments arranged by size: 

                 837     195      74      58
 
NspI r_CATG'y

Cuts at:      0     390    1080    1164 
   Size:        390     690      84

  Fragments arranged by size: 

                 690     390      84
 
PciI A'CATG_T

Cuts at:      0     386    1164 
   Size:        386     778
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     585    1164 
   Size:        585     579
 
PfoI T'CCnGG_A

Cuts at:      0     995    1164 
   Size:        995     169
 
PmlI CAC'GTG

Cuts at:      0     882    1164 
   Size:        882     282
 
PspOMI G'GGCC_C

Cuts at:      0     112    1164 
   Size:        112    1052
 
PstI C_TGCA'G

Cuts at:      0      66     602    1164 
   Size:         66     536     562

  Fragments arranged by size: 

                 562     536      66
 
PvuII CAG'CTG

Cuts at:      0     837     911    1164 
   Size:        837      74     253

  Fragments arranged by size: 

                 837     253      74
 
SacI G_AGCT'C

Cuts at:      0     284    1164 
   Size:        284     880
 
SalI G'TCGA_C

Cuts at:      0     373    1164 
   Size:        373     791
 
SapI GCTCTTCn'nnn_

Cuts at:      0     289    1164 
   Size:        289     875
 
SfcI C'TryA_G

Cuts at:      0      62     598    1164 
   Size:         62     536     566

  Fragments arranged by size: 

                 566     536      62
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     316    1164 
   Size:        316     848
 
SmaI CCC'GGG

Cuts at:      0      27     117    1164 
   Size:         27      90    1047

  Fragments arranged by size: 

                1047      90      27
 
SmlI C'TyrA_G

Cuts at:      0    1051    1164 
   Size:       1051     113
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     614    1164 
   Size:        614     550
 
XmaI C'CCGG_G

Cuts at:      0      25     115    1164 
   Size:         25      90    1049

  Fragments arranged by size: 

                1049      90      25
 

 Enzymes that do cut:

     AccI     AfeI   AflIII    AlwNI     ApaI    ApaLI     AvaI    BanII
     BbsI    BbvCI     BglI     BlpI Bme1580I     BpmI   Bpu10I    BpuEI
    BsaAI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI
    BsrFI   BssHII   BstAPI     BtgI    BtgZI     BtsI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    HaeII   HincII     MmeI
     MscI     MslI   MspA1I     NspI     PciI    PflMI     PfoI     PmlI
   PspOMI     PstI    PvuII     SacI     SalI     SapI     SfcI     SfiI
     SmaI     SmlI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmrI     BmtI     BplI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII    SanDI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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