Restriction map for
pFN21AB3917
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# NO CUT ENZYMES ( 67 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp718I AspEI AspI AviII BbrPI
BfrI BseAI BsiWI BspDI BspEI
Bst1107I BstAPI BstEII BstPI BstZ17I
Bsu36I ClaI DrdI Eam1105I Eco81I
EcoNI EcoO65I EcoRV FseI FspI
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI NheI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
SacII SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 3892 GACGTC GACGT^C
Asp700I 3927 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 942 CCTAGG C^CTAGG
BbeI 3259 GGCGCC GGCGC^C
BciVI 760 GGATAC GTATCC(6/5)
BlnI 942 CCTAGG C^CTAGG
BlpI 604 GCTAAGC GC^T[ACGT]AGC
BpmI 1261 CTGGAG CTGGAG(16/14)
Bpu1102I 604 GCTAAGC GC^T[ACGT]AGC
BsaAI 4830 CACGTA [CT]AC^GT[GA]
BsaWI 4985 TCCGGT [AT]^CCGG[AT]
BsmBI 1772 GAGACG CGTCTC(1/5)
Bsp1407I 1346 TGTACA T^GTACA
BspHI 992 TCATGA T^CATGA
BsrBI 2949 CCGCTC CCGCTC(-3/-3)
BsrGI 1346 TGTACA T^GTACA
BssHII 1321 GCGCGC G^CGCGC
BssSI 2736 CTCGTG CACGAG(-5/-1)
BstBI 1013 TTCGAA TT^CGAA
BstXI 5051 CCACCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 604 GCTAAGC GC^T[ACGT]AGC
CpoI 3017 CGGTCCG CG^G[AT]CCG
DraI 4039 TTTAAA TTT^AAA
EagI 3416 CGGCCG C^GGCCG
EclXI 3416 CGGCCG C^GGCCG
Eco52I 3416 CGGCCG C^GGCCG
KasI 3259 GGCGCC G^GCGCC
NaeI 1616 GCCGGC GCC^GGC
NarI 3259 GGCGCC GG^CGCC
NgoMIV 1616 GCCGGC G^CCGGC
NotI 3415 GCGGCCGC GC^GGCCGC
NspV 1013 TTCGAA TT^CGAA
RcaI 992 TCATGA T^CATGA
RsrII 3017 CGGTCCG CG^G[AT]CCG
SalI 4906 GTCGAC G^TCGAC
SfoI 3259 GGCGCC GGC^GCC
SfuI 1013 TTCGAA TT^CGAA
SspBI 1346 TGTACA T^GTACA
SspI 1195 AATATT AAT^ATT
XbaI 894 TCTAGA T^CTAGA
XmnI 3927 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 403 GTCTAC GT^[AC][GT]AC
4906 GTCGAC
Alw44I 691 GTGCAC G^TGCAC
1468 GTGCAC
ApaLI 691 GTGCAC G^TGCAC
1468 GTGCAC
BamHI 4982 GGATCC G^GATCC
5062 GGATCC
BbsI 2398 GAAGAC GAAGAC(2/6)
4327 GAAGAC
BclI 1842 TGATCA T^GATCA
2210 TGATCA
BglII 2300 AGATCT A^GATCT
2732 AGATCT
BpuAI 2398 GAAGAC GAAGAC(2/6)
4327 GAAGAC
BsiEI 3416 CGGCCG CG[GA][CT]^CG
4904 CGGTCG
BspLU11I 537 ACATGT A^CATGT
1343 ACATGT
BsrFI 1616 GCCGGC [GA]^CCGG[CT]
2869 ACCGGC
Cfr10I 1616 GCCGGC [GA]^CCGG[CT]
2869 ACCGGC
EcoRI 419 GAATTC G^AATTC
1370 GAATTC
EcoT22I 2637 ATGCAT ATGCA^T
3971 ATGCAT
FbaI 1842 TGATCA T^GATCA
2210 TGATCA
NdeI 595 CATATG CA^TATG
2513 CATATG
NsiI 2637 ATGCAT ATGCA^T
3971 ATGCAT
PflMI 1872 CCAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2898 CCACTGCATGG
PshAI 3430 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3505 GACTACAGTC
SapI 67 GAAGAGC GCTCTTC(1/4)
4109 GAAGAGC
SmaI 338 CCCGGG CCC^GGG
1946 CCCGGG
SphI 2635 GCATGC GCATG^C
2639 GCATGC
Van91I 1872 CCAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2898 CCACTGCATGG
XcmI 1411 CCAGTTACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3857 CCAGGGTGTGCTTGG
XmaCI 338 CCCGGG C^CCGGG
1946 CCCGGG
XmaI 338 CCCGGG C^CCGGG
1946 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco47III EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII