(Circular) (Six-base) MAPSORT of: pFN21AB3917.seq Check: 6122 from: 1 to: 9865 pFN21AB3917 9865 bp With 182 enzymes: SgfI * March 4, 2015 12:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2210 2210 Size: 9865 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5901 7675 278 Size: 53 83 186 936 4365 1774 2468 Fragments arranged by size: 4365 2468 1774 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2409 6912 7208 1937 Size: 472 4503 296 4594 Fragments arranged by size: 4594 4503 472 296 Acc65I G'GTAC_C Cuts at: 7186 7186 Size: 9865 AclI AA'CG_TT Cuts at: 1563 7992 8365 1563 Size: 6429 373 3063 Fragments arranged by size: 6429 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2091 2200 3170 3735 4052 4613 5194 5618 Size: 459 109 970 565 317 561 581 424 Cuts at: 5618 5849 5867 6234 7067 7928 9312 9580 1632 Size: 231 18 367 833 861 1384 268 1917 Fragments arranged by size: 1917 1384 970 861 833 581 565 561 459 424 367 317 268 231 109 18 AfeI AGC'GCT Cuts at: 2615 3529 5609 2615 Size: 914 2080 6871 Fragments arranged by size: 6871 2080 914 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9668 AflIII A'CryG_T Cuts at: 1203 2542 2553 3348 6834 8764 9741 1203 Size: 1339 11 795 3486 1930 977 1327 Fragments arranged by size: 3486 1930 1339 1327 977 795 11 AgeI A'CCGG_T Cuts at: 8696 8696 Size: 9865 AhdI GACnn_n'nnGTC Cuts at: 8594 8594 Size: 9865 AleI CACnn'nnGTG Cuts at: 1166 2204 4940 5864 1166 Size: 1038 2736 924 5167 Fragments arranged by size: 5167 2736 1038 924 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4823 5063 1184 Size: 3639 240 5986 Fragments arranged by size: 5986 3639 240 AlwNI CAG_nnn'CTG Cuts at: 2223 3265 3571 5056 5098 5968 7045 7159 7608 Size: 1042 306 1485 42 870 1077 114 449 Cuts at: 7608 9180 2223 Size: 1572 2908 Fragments arranged by size: 2908 1572 1485 1077 1042 870 449 306 114 42 ApaI G_GGCC'C Cuts at: 2174 2398 4476 6489 2174 Size: 224 2078 2013 5550 Fragments arranged by size: 5550 2078 2013 224 ApaLI G'TGCA_C Cuts at: 2696 3473 7922 9078 2696 Size: 777 4449 1156 3483 Fragments arranged by size: 4449 3483 1156 777 ApoI r'AATT_y Cuts at: 1493 2424 2472 3375 5289 5936 7174 7400 7552 Size: 931 48 903 1914 647 1238 226 152 Cuts at: 7552 9661 1493 Size: 2109 1697 Fragments arranged by size: 2109 1914 1697 1238 931 903 647 226 152 48 AseI AT'TA_AT Cuts at: 160 1032 8419 160 Size: 872 7387 1606 Fragments arranged by size: 7387 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9865 AvaI C'yCGr_G Cuts at: 1870 1943 2343 3933 3951 6008 6488 7190 1870 Size: 73 400 1590 18 2057 480 702 4545 Fragments arranged by size: 4545 2057 1590 702 480 400 73 18 AvrII C'CTAG_G Cuts at: 2947 2947 Size: 9865 BamHI G'GATC_C Cuts at: 6987 7067 7195 7562 6987 Size: 80 128 367 9290 Fragments arranged by size: 9290 367 128 80 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2232 2914 3589 5264 6576 Size: 325 217 627 445 682 675 1675 1312 Cuts at: 6576 6653 6687 7186 8641 618 Size: 77 34 499 1455 1842 Fragments arranged by size: 1842 1675 1455 1312 682 675 627 499 445 325 217 77 34 BanII G_rGCy'C Cuts at: 1391 2079 2174 2253 2312 2398 2730 4274 4476 Size: 688 95 79 59 86 332 1544 202 Cuts at: 4476 5670 6480 6489 6629 7149 7184 1391 Size: 1194 810 9 140 520 35 4072 Fragments arranged by size: 4072 1544 1194 810 688 520 332 202 140 95 86 79 59 35 9 BbeI G_GCGC'C Cuts at: 5268 5268 Size: 9865 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4410 6339 928 Size: 971 2511 1929 4454 Fragments arranged by size: 4454 2511 1929 971 BbvCI CC'TCA_GC Cuts at: 2375 5366 6219 7082 2375 Size: 2991 853 863 5158 Fragments arranged by size: 5158 2991 863 853 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2035 2069 2444 2478 3770 3804 6892 Size: 34 98 34 375 34 1292 34 3088 Cuts at: 6892 6926 8056 8090 1903 Size: 34 1130 34 3678 Fragments arranged by size: 3678 3088 1292 1130 375 98 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2759 7757 8967 2759 Size: 4998 1210 3657 Fragments arranged by size: 4998 3657 1210 BclI T'GATC_A Cuts at: 1548 3847 4215 1548 Size: 2299 368 7198 Fragments arranged by size: 7198 2299 368 BfrBI ATG'CAT Cuts at: 4644 5978 4644 Size: 1334 8531 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2032 3587 4752 Size: 107 122 71 631 605 360 1555 1165 Cuts at: 4752 4959 6484 8476 136 Size: 207 1525 1992 1525 Fragments arranged by size: 1992 1555 1525 1525 1165 631 605 360 207 122 107 71 BglII A'GATC_T Cuts at: 4305 4737 9860 4305 Size: 432 5123 4310 Fragments arranged by size: 5123 4310 432 BlpI GC'TnA_GC Cuts at: 2610 7282 2610 Size: 4672 5193 Bme1580I G_kGCm'C Cuts at: 1790 2174 2235 2398 2700 2788 3477 3592 3703 Size: 384 61 163 302 88 689 115 111 Cuts at: 3703 4476 5805 6201 6489 7926 9082 1790 Size: 773 1329 396 288 1437 1156 2573 Fragments arranged by size: 2573 1437 1329 1156 773 689 396 384 302 288 163 115 111 88 61 BmgBI CAC'GTC Cuts at: 1318 3196 1318 Size: 1878 7987 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2880 2930 4149 Size: 416 53 83 399 24 1463 50 1219 Cuts at: 4149 5433 7596 8554 442 Size: 1284 2163 958 1753 Fragments arranged by size: 2163 1753 1463 1284 1219 958 416 399 83 53 50 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9865 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2212 2212 Size: 9865 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3287 8525 1239 Size: 2048 5238 2579 Fragments arranged by size: 5238 2579 2048 Bpu10I CC'TnA_GC Cuts at: 2375 3524 3594 4957 5366 6219 6406 7082 2375 Size: 1149 70 1363 409 853 187 676 5158 Fragments arranged by size: 5158 1363 1149 853 676 409 187 70 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2063 2438 2908 5293 5339 7133 7990 8855 9153 Size: 375 470 2385 46 1794 857 865 298 Cuts at: 9153 2063 Size: 2775 Fragments arranged by size: 2775 2385 1794 865 857 470 375 298 46 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2946 4313 4771 5998 7092 8528 882 Size: 617 1447 1367 458 1227 1094 1436 2219 Fragments arranged by size: 2219 1447 1436 1367 1227 1094 617 458 BsaAI yAC'GTr Cuts at: 493 1204 6837 7656 493 Size: 711 5633 819 2702 Fragments arranged by size: 5633 2702 819 711 BsaBI GATnn'nnATC Cuts at: 4551 4736 6608 9859 4551 Size: 185 1872 3251 4557 Fragments arranged by size: 4557 3251 1872 185 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4793 5265 5455 5898 Size: 53 83 186 936 3260 472 190 443 Cuts at: 5898 6231 7672 8054 8689 275 Size: 333 1441 382 635 1451 Fragments arranged by size: 3260 1451 1441 936 635 472 443 382 333 190 186 83 53 BsaWI w'CCGG_w Cuts at: 6990 8298 8696 8970 9117 9528 6990 Size: 1308 398 274 147 411 7327 Fragments arranged by size: 7327 1308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3665 3695 3959 3989 5145 Size: 30 165 30 2262 30 264 30 1156 Cuts at: 5145 5175 6410 6440 6557 6587 6611 6641 1178 Size: 30 1235 30 117 30 24 30 4402 Fragments arranged by size: 4402 2262 1235 1156 264 165 117 30 30 30 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 4155 6172 6206 6236 6284 6287 6314 Size: 1081 1888 2017 34 30 48 3 27 Cuts at: 6314 6317 6332 6431 6578 1186 Size: 3 15 99 147 4473 Fragments arranged by size: 4473 2017 1888 1081 147 99 48 34 30 27 15 3 3 BseYI C'CCAG_C Cuts at: 3543 3577 3811 4750 6143 7063 9068 9564 3543 Size: 34 234 939 1393 920 2005 496 3844 Fragments arranged by size: 3844 2005 1393 939 920 496 234 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 2531 3656 3826 4718 5027 5069 Size: 573 492 303 1125 170 892 309 42 Cuts at: 5069 1163 Size: 5959 Fragments arranged by size: 5959 1125 892 573 492 309 303 170 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5424 6912 7315 8076 9104 1540 Size: 275 186 3423 1488 403 761 1028 2301 Fragments arranged by size: 3423 2301 1488 1028 761 403 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2198 2253 2700 3477 3960 4274 5811 6373 Size: 119 55 447 777 483 314 1537 562 Cuts at: 6373 6629 7149 7926 8011 9082 2079 Size: 256 520 777 85 1071 2862 Fragments arranged by size: 2862 1537 1071 777 777 562 520 483 447 314 256 119 85 55 BsmI GAATG_Cn' Cuts at: 2611 3793 5736 7376 7469 2611 Size: 1182 1943 1640 93 5007 Fragments arranged by size: 5007 1943 1640 1182 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3771 3771 Size: 9865 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2174 2198 2235 2253 2312 2398 Size: 399 289 95 24 37 18 59 86 Cuts at: 2398 2700 2730 2788 3477 3592 3703 3960 4274 Size: 302 30 58 689 115 111 257 314 Cuts at: 4274 4476 5670 5805 5811 6201 6373 6480 6489 Size: 202 1194 135 6 390 172 107 9 Cuts at: 6489 6629 7149 7184 7926 8011 9082 1391 Size: 140 520 35 742 85 1071 2174 Fragments arranged by size: 2174 1194 1071 742 689 520 399 390 314 302 289 257 202 172 140 135 115 111 107 95 86 85 59 58 37 35 30 24 18 9 6 BspEI T'CCGG_A Cuts at: 9528 9528 Size: 9865 BspHI T'CATG_A Cuts at: 2997 7754 9484 2997 Size: 4757 1730 3378 Fragments arranged by size: 4757 3378 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2210 2304 4349 4377 4509 4854 7220 844 Size: 1366 94 2045 28 132 345 2366 3489 Fragments arranged by size: 3489 2366 2045 1366 345 132 94 28 BsrBI CCG'CTC Cuts at: 1394 1823 4956 7752 1394 Size: 429 3133 2796 3507 Fragments arranged by size: 3507 3133 2796 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2577 4630 5341 8360 8534 65 Size: 1183 1329 2053 711 3019 174 1396 Fragments arranged by size: 3019 2053 1396 1329 1183 711 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3621 4874 8509 8696 1700 Size: 110 1811 1253 3635 187 2869 Fragments arranged by size: 3635 2869 1811 1253 187 110 BsrGI T'GTAC_A Cuts at: 96 3351 96 Size: 3255 6610 BssHII G'CGCG_C Cuts at: 1925 3326 1925 Size: 1401 8464 BssSI C'ACGA_G Cuts at: 4741 7925 8937 4741 Size: 3184 1012 5669 Fragments arranged by size: 5669 3184 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9865 BstBI TT'CG_AA Cuts at: 3019 8669 3019 Size: 5650 4215 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9865 BstXI CCAn_nnnn'nTGG Cuts at: 7063 7063 Size: 9865 BstYI r'GATC_y Cuts at: 1972 3261 4305 4737 5185 6130 6525 6790 6987 Size: 1289 1044 432 448 945 395 265 197 Cuts at: 6987 7067 7195 7562 7947 7964 9416 9502 9514 Size: 80 128 367 385 17 1452 86 12 Cuts at: 9514 9860 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1289 1044 945 448 432 395 385 367 346 265 197 128 86 80 17 12 BtgI C'CryG_G Cuts at: 513 1065 2691 3381 3425 3489 3555 3582 6495 Size: 552 1626 690 44 64 66 27 2913 Cuts at: 6495 6649 513 Size: 154 3729 Fragments arranged by size: 3729 2913 1626 690 552 154 66 64 44 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6889 487 Size: 683 56 5663 3463 Fragments arranged by size: 5663 3463 683 56 BtsI GCAGTG_nn' Cuts at: 2623 3668 4291 4465 4805 4903 4935 5047 7381 Size: 1045 623 174 340 98 32 112 2334 Cuts at: 7381 8175 8195 2623 Size: 794 20 4293 Fragments arranged by size: 4293 2334 1045 794 623 340 174 112 98 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9865 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9830 DraI TTT'AAA Cuts at: 6046 7169 6046 Size: 1123 8742 DraIII CAC_nnn'GTG Cuts at: 5618 6205 6687 5618 Size: 587 482 8796 Fragments arranged by size: 8796 587 482 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8872 809 Size: 507 7556 1802 Fragments arranged by size: 7556 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2710 2971 3069 Size: 54 1425 50 275 18 880 261 98 Cuts at: 3069 3558 3874 5124 5338 5421 5766 7312 8201 Size: 489 316 1250 214 83 345 1546 889 Cuts at: 8201 9830 8 Size: 1629 43 Fragments arranged by size: 1629 1546 1425 1250 889 880 489 345 316 275 261 214 98 83 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5421 7312 1537 Size: 275 3609 1891 4090 Fragments arranged by size: 4090 3609 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 2285 2591 3711 4361 5254 5537 5825 Size: 714 218 306 1120 650 893 283 288 Cuts at: 5825 6109 6183 6291 6390 7006 7795 1353 Size: 284 74 108 99 616 789 3423 Fragments arranged by size: 3423 1120 893 789 714 650 616 306 288 284 283 218 108 99 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 8452 8826 8972 387 Size: 1064 138 2675 4188 374 146 1280 Fragments arranged by size: 4188 2675 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2077 2251 4272 6627 7147 2077 Size: 174 2021 2355 520 4795 Fragments arranged by size: 4795 2355 2021 520 174 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2091 2200 3170 3287 3735 4052 4613 Size: 393 459 109 970 117 448 317 561 Cuts at: 4613 5194 5618 5849 5867 6234 7067 7928 8525 Size: 581 424 231 18 367 833 861 597 Cuts at: 8525 9312 9580 1239 Size: 787 268 1524 Fragments arranged by size: 1524 970 861 833 787 597 581 561 459 448 424 393 367 317 268 231 117 109 18 EcoO109I rG'GnC_Cy Cuts at: 2171 2394 2395 2529 4473 4924 5583 6421 6485 Size: 223 1 134 1944 451 659 838 64 Cuts at: 6485 6784 2171 Size: 299 5252 Fragments arranged by size: 5252 1944 838 659 451 299 223 134 64 1 EcoRI G'AATT_C Cuts at: 2424 3375 7174 7552 2424 Size: 951 3799 378 4737 Fragments arranged by size: 4737 3799 951 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6631 6631 Size: 9865 FspI TGC'GCA Cuts at: 8371 8371 Size: 9865 HaeII r_GCGC'y Cuts at: 1678 2617 3531 5268 5611 5775 6505 9012 1678 Size: 939 914 1737 343 164 730 2507 2531 Fragments arranged by size: 2531 2507 1737 939 914 730 343 164 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2631 2663 3669 3701 3886 Size: 32 135 32 1059 32 1006 32 185 Cuts at: 3886 3918 6410 6442 6611 6643 7070 7102 8506 Size: 32 2492 32 169 32 427 32 1404 Cuts at: 8506 8538 8580 8612 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2492 1404 1059 1006 427 185 169 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2673 4071 6913 7209 7455 669 Size: 988 281 735 1398 2842 296 246 3079 Fragments arranged by size: 3079 2842 1398 988 735 296 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9865 HpaI GTT'AAC Cuts at: 7455 7455 Size: 9865 KasI G'GCGC_C Cuts at: 5264 5264 Size: 9865 KpnI G_GTAC'C Cuts at: 7190 7190 Size: 9865 MfeI C'AATT_G Cuts at: 7464 7464 Size: 9865 MluI A'CGCG_T Cuts at: 9741 9741 Size: 9865 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4754 5364 6834 8979 9163 1305 Size: 3449 610 1470 2145 184 2007 Fragments arranged by size: 3449 2145 2007 1470 610 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2712 2973 3071 3560 3876 Size: 54 1425 343 880 261 98 489 316 Cuts at: 3876 5126 5768 10 Size: 1250 642 4107 Fragments arranged by size: 4107 1425 1250 880 642 489 343 316 261 98 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 2604 4412 4940 5163 5678 5864 Size: 648 1038 400 1808 528 223 515 186 Cuts at: 5864 7823 8182 8341 518 Size: 1959 359 159 2042 Fragments arranged by size: 2042 1959 1808 1038 648 528 515 400 359 223 186 159 MspA1I CmG'CkG Cuts at: 1606 2374 2708 4045 4326 4786 5596 5953 6127 Size: 768 334 1337 281 460 810 357 174 Cuts at: 6127 7281 7958 9106 9351 1606 Size: 1154 677 1148 245 2120 Fragments arranged by size: 2120 1337 1154 1148 810 768 677 460 357 334 281 245 174 NaeI GCC'GGC Cuts at: 3623 3623 Size: 9865 NarI GG'CG_CC Cuts at: 5265 5265 Size: 9865 NcoI C'CATG_G Cuts at: 513 1065 2691 3381 3425 3489 6495 6649 513 Size: 552 1626 690 44 64 3006 154 3729 Fragments arranged by size: 3729 3006 1626 690 552 154 64 44 NdeI CA'TA_TG Cuts at: 387 2601 4519 387 Size: 2214 1918 5733 Fragments arranged by size: 5733 2214 1918 NgoMIV G'CCGG_C Cuts at: 3621 3621 Size: 9865 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9865 NotI GC'GGCC_GC Cuts at: 5421 7312 5421 Size: 1891 7974 NsiI A_TGCA'T Cuts at: 4646 5980 4646 Size: 1334 8531 NspI r_CATG'y Cuts at: 1132 1229 2026 2332 2546 3352 3629 4644 4648 Size: 97 797 306 214 806 277 1015 4 Cuts at: 4648 5978 6340 7223 8768 1132 Size: 1330 362 883 1545 2229 Fragments arranged by size: 2229 1545 1330 1015 883 806 797 362 306 277 214 97 4 PasI CC'CwG_GG Cuts at: 1348 2533 2731 3601 4839 5348 5731 6481 1348 Size: 1185 198 870 1238 509 383 750 4732 Fragments arranged by size: 4732 1238 1185 870 750 509 383 198 PciI A'CATG_T Cuts at: 2542 3348 8764 2542 Size: 806 5416 3643 Fragments arranged by size: 5416 3643 806 PflMI CCAn_nnn'nTGG Cuts at: 1108 3883 4909 1108 Size: 2775 1026 6064 Fragments arranged by size: 6064 2775 1026 PfoI T'CCnGG_A Cuts at: 3264 3897 5901 6786 3264 Size: 633 2004 885 6343 Fragments arranged by size: 6343 2004 885 633 PmeI GTTT'AAAC Cuts at: 7169 7169 Size: 9865 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7934 7966 9447 9479 7934 Size: 32 1481 32 8320 Fragments arranged by size: 8320 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2529 5583 6421 6784 2529 Size: 3054 838 363 5610 Fragments arranged by size: 5610 3054 838 363 PshAI GACnn'nnGTC Cuts at: 1613 5439 5514 1613 Size: 3826 75 5964 Fragments arranged by size: 5964 3826 75 PsiI TTA'TAA Cuts at: 7435 7435 Size: 9865 PspOMI G'GGCC_C Cuts at: 2170 2394 4472 6485 2170 Size: 224 2078 2013 5550 Fragments arranged by size: 5550 2078 2013 224 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2099 2131 2099 Size: 32 9833 PstI C_TGCA'G Cuts at: 830 2043 2179 4374 4689 5103 5595 7217 830 Size: 1213 136 2195 315 414 492 1622 3478 Fragments arranged by size: 3478 2195 1622 1213 492 414 315 136 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9865 PvuII CAG'CTG Cuts at: 2374 2708 5596 5953 2374 Size: 334 2888 357 6286 Fragments arranged by size: 6286 2888 357 334 RsrII CG'GwC_CG Cuts at: 5023 5023 Size: 9865 SacI G_AGCT'C Cuts at: 2079 2253 4274 6629 7149 2079 Size: 174 2021 2355 520 4795 Fragments arranged by size: 4795 2355 2021 520 174 SalI G'TCGA_C Cuts at: 1936 6911 7207 1936 Size: 4975 296 4594 Fragments arranged by size: 4975 4594 296 SanDI GG'GwC_CC Cuts at: 2529 2529 Size: 9865 SapI GCTCTTCn'nnn_ Cuts at: 2067 6109 2067 Size: 4042 5823 SbfI CC_TGCA'GG Cuts at: 7217 7217 Size: 9865 ScaI AGT'ACT Cuts at: 8113 9825 8113 Size: 1712 8153 SfcI C'TryA_G Cuts at: 826 1045 2039 2175 3081 4370 4378 4685 5099 Size: 219 994 136 906 1289 8 307 414 Cuts at: 5099 5512 5591 7010 7213 8348 9029 9220 826 Size: 413 79 1419 203 1135 681 191 1471 Fragments arranged by size: 1471 1419 1289 1135 994 906 681 414 413 307 219 203 191 136 79 8 SfoI GGC'GCC Cuts at: 5266 5266 Size: 9865 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9865 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9865 SmaI CCC'GGG Cuts at: 1872 2345 3953 7192 1872 Size: 473 1608 3239 4545 Fragments arranged by size: 4545 3239 1608 473 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 2417 2923 5272 5318 7148 Size: 197 926 135 339 506 2349 46 1830 Cuts at: 7148 7969 8870 9132 820 Size: 821 901 262 1553 Fragments arranged by size: 2349 1830 1553 926 901 821 506 339 262 197 135 46 SnaBI TAC'GTA Cuts at: 493 7656 493 Size: 7163 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9865 SphI G_CATG'C Cuts at: 1132 4644 4648 7223 1132 Size: 3512 4 2575 3774 Fragments arranged by size: 3774 3512 2575 4 SspI AAT'ATT Cuts at: 5 52 3202 7789 5 Size: 47 3150 4587 2081 Fragments arranged by size: 4587 3150 2081 47 StuI AGG'CCT Cuts at: 1517 3282 3755 3827 7137 1517 Size: 1765 473 72 3310 4245 Fragments arranged by size: 4245 3310 1765 473 72 StyI C'CwwG_G Cuts at: 513 1065 2691 2947 3381 3425 3489 3867 4392 Size: 552 1626 256 434 44 64 378 525 Cuts at: 4392 5741 6495 6649 6672 7304 513 Size: 1349 754 154 23 632 3074 Fragments arranged by size: 3074 1626 1349 754 632 552 525 434 378 256 154 64 44 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2791 3091 3132 3492 4141 4478 5690 Size: 1255 1211 300 41 360 649 337 1212 Cuts at: 5690 5803 6479 7892 7909 8062 8247 8692 325 Size: 113 676 1413 17 153 185 445 1498 Fragments arranged by size: 1498 1413 1255 1212 1211 676 649 445 360 337 300 185 153 113 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3131 3351 8111 Size: 274 80 33 51 1443 1154 220 4760 Cuts at: 8111 9823 96 Size: 1712 138 Fragments arranged by size: 4760 1712 1443 1154 274 220 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3297 3329 3665 3697 5145 5177 6557 6589 9825 Size: 32 336 32 1448 32 1380 32 3236 Cuts at: 9825 9857 3297 Size: 32 3305 Fragments arranged by size: 3305 3236 1448 1380 336 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9865 XbaI T'CTAG_A Cuts at: 2899 7201 2899 Size: 4302 5563 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3423 5869 3423 Size: 2446 7419 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9865 XmaI C'CCGG_G Cuts at: 1870 2343 3951 7190 1870 Size: 473 1608 3239 4545 Fragments arranged by size: 4545 3239 1608 473 XmnI GAAnn'nnTTC Cuts at: 5936 7994 5936 Size: 2058 7807 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5899 7673 276 Size: 53 83 186 936 4365 1774 2468 Fragments arranged by size: 4365 2468 1774 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI PacI PmlI PspXI SacII SexAI SfiI SrfI SwaI