(Linear) (Six-base) MAPSORT of: KIBB3917.seq Check: 1471 from: 1 to: 5160 KIBB3917.orf With 182 enzymes: SgfI * March 4, 2015 11:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 205 5160 Size: 205 4955 AatII G_ACGT'C Cuts at: 0 3896 5160 Size: 3896 1264 AccI GT'mk_AC Cuts at: 0 404 4907 5160 Size: 404 4503 253 Fragments arranged by size: 4503 404 253 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 86 195 1165 1730 2047 2608 3189 3613 Size: 86 109 970 565 317 561 581 424 Cuts at: 3613 3844 3862 4229 5062 5160 Size: 231 18 367 833 98 Fragments arranged by size: 970 833 581 565 561 424 367 317 231 109 98 86 18 AfeI AGC'GCT Cuts at: 0 610 1524 3604 5160 Size: 610 914 2080 1556 Fragments arranged by size: 2080 1556 914 610 AflIII A'CryG_T Cuts at: 0 537 548 1343 4829 5160 Size: 537 11 795 3486 331 Fragments arranged by size: 3486 795 537 331 11 AleI CACnn'nnGTG Cuts at: 0 199 2935 3859 5160 Size: 199 2736 924 1301 Fragments arranged by size: 2736 1301 924 199 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2818 3058 5160 Size: 2818 240 2102 Fragments arranged by size: 2818 2102 240 AlwNI CAG_nnn'CTG Cuts at: 0 218 1260 1566 3051 3093 3963 5040 5154 Size: 218 1042 306 1485 42 870 1077 114 Cuts at: 5154 5160 Size: 6 Fragments arranged by size: 1485 1077 1042 870 306 218 114 42 6 ApaI G_GGCC'C Cuts at: 0 169 393 2471 4484 5160 Size: 169 224 2078 2013 676 Fragments arranged by size: 2078 2013 676 224 169 ApaLI G'TGCA_C Cuts at: 0 691 1468 5160 Size: 691 777 3692 Fragments arranged by size: 3692 777 691 ApoI r'AATT_y Cuts at: 0 419 467 1370 3284 3931 5160 Size: 419 48 903 1914 647 1229 Fragments arranged by size: 1914 1229 903 647 419 48 AvaI C'yCGr_G Cuts at: 0 338 1928 1946 4003 4483 5160 Size: 338 1590 18 2057 480 677 Fragments arranged by size: 2057 1590 677 480 338 18 AvrII C'CTAG_G Cuts at: 0 942 5160 Size: 942 4218 BamHI G'GATC_C Cuts at: 0 4982 5062 5160 Size: 4982 80 98 Fragments arranged by size: 4982 98 80 BanI G'GyrC_C Cuts at: 0 227 909 1584 3259 4571 4648 4682 5160 Size: 227 682 675 1675 1312 77 34 478 Fragments arranged by size: 1675 1312 682 675 478 227 77 34 BanII G_rGCy'C Cuts at: 0 74 169 248 307 393 725 2269 2471 Size: 74 95 79 59 86 332 1544 202 Cuts at: 2471 3665 4475 4484 4624 5144 5160 Size: 1194 810 9 140 520 16 Fragments arranged by size: 1544 1194 810 520 332 202 140 95 86 79 74 59 16 9 BbeI G_GCGC'C Cuts at: 0 3263 5160 Size: 3263 1897 BbsI GAAGACnn'nnnn_ Cuts at: 0 2405 4334 5160 Size: 2405 1929 826 Fragments arranged by size: 2405 1929 826 BbvCI CC'TCA_GC Cuts at: 0 370 3361 4214 5077 5160 Size: 370 2991 853 863 83 Fragments arranged by size: 2991 863 853 370 83 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 30 64 439 473 1765 1799 4887 4921 Size: 30 34 375 34 1292 34 3088 34 Cuts at: 4921 5160 Size: 239 Fragments arranged by size: 3088 1292 375 239 34 34 34 34 30 BciVI GTATCCnnnnn_n' Cuts at: 0 754 5160 Size: 754 4406 BclI T'GATC_A Cuts at: 0 1842 2210 5160 Size: 1842 368 2950 Fragments arranged by size: 2950 1842 368 BfrBI ATG'CAT Cuts at: 0 2639 3973 5160 Size: 2639 1334 1187 Fragments arranged by size: 2639 1334 1187 BglI GCCn_nnn'nGGC Cuts at: 0 27 1582 2747 2954 4479 5160 Size: 27 1555 1165 207 1525 681 Fragments arranged by size: 1555 1525 1165 681 207 27 BglII A'GATC_T Cuts at: 0 2300 2732 5160 Size: 2300 432 2428 Fragments arranged by size: 2428 2300 432 BlpI GC'TnA_GC Cuts at: 0 605 5160 Size: 605 4555 Bme1580I G_kGCm'C Cuts at: 0 169 230 393 695 783 1472 1587 1698 Size: 169 61 163 302 88 689 115 111 Cuts at: 1698 2471 3800 4196 4484 5160 Size: 773 1329 396 288 676 Fragments arranged by size: 1329 773 689 676 396 302 288 169 163 115 111 88 61 BmgBI CAC'GTC Cuts at: 0 1191 5160 Size: 1191 3969 BmrI ACTGGGnnnn_n' Cuts at: 0 875 925 2144 3428 5160 Size: 875 50 1219 1284 1732 Fragments arranged by size: 1732 1284 1219 875 50 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 207 5160 Size: 207 4953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1282 5160 Size: 1282 3878 Bpu10I CC'TnA_GC Cuts at: 0 370 1519 1589 2952 3361 4214 4401 5077 Size: 370 1149 70 1363 409 853 187 676 Cuts at: 5077 5160 Size: 83 Fragments arranged by size: 1363 1149 853 676 409 370 187 83 70 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 433 903 3288 3334 5128 5160 Size: 58 375 470 2385 46 1794 32 Fragments arranged by size: 2385 1794 470 375 58 46 32 BsaI GGTCTCn'nnnn_ Cuts at: 0 941 2308 2766 3993 5087 5160 Size: 941 1367 458 1227 1094 73 Fragments arranged by size: 1367 1227 1094 941 458 73 BsaAI yAC'GTr Cuts at: 0 4832 5160 Size: 4832 328 BsaBI GATnn'nnATC Cuts at: 0 2546 2731 4603 5160 Size: 2546 185 1872 557 Fragments arranged by size: 2546 1872 557 185 BsaHI Gr'CG_yC Cuts at: 0 2788 3260 3450 3893 4226 5160 Size: 2788 472 190 443 333 934 Fragments arranged by size: 2788 934 472 443 333 190 BsaWI w'CCGG_w Cuts at: 0 4985 5160 Size: 4985 175 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1660 1690 1954 1984 3140 3170 4405 4435 Size: 1660 30 264 30 1156 30 1235 30 Cuts at: 4435 4552 4582 4606 4636 5160 Size: 117 30 24 30 524 Fragments arranged by size: 1660 1235 1156 524 264 117 30 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 262 2150 4167 4201 4231 4279 4282 4309 Size: 262 1888 2017 34 30 48 3 27 Cuts at: 4309 4312 4327 4426 4573 5160 Size: 3 15 99 147 587 Fragments arranged by size: 2017 1888 587 262 147 99 48 34 30 27 15 3 3 BseYI C'CCAG_C Cuts at: 0 1538 1572 1806 2745 4138 5058 5160 Size: 1538 34 234 939 1393 920 102 Fragments arranged by size: 1538 1393 939 920 234 102 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 223 526 1651 1821 2713 3022 3064 5160 Size: 223 303 1125 170 892 309 42 2096 Fragments arranged by size: 2096 1125 892 309 303 223 170 42 BsiEI CG_ry'CG Cuts at: 0 3419 4907 5160 Size: 3419 1488 253 Fragments arranged by size: 3419 1488 253 BsiHKAI G_wGCw'C Cuts at: 0 74 193 248 695 1472 1955 2269 3806 Size: 74 119 55 447 777 483 314 1537 Cuts at: 3806 4368 4624 5144 5160 Size: 562 256 520 16 Fragments arranged by size: 1537 777 562 520 483 447 314 256 119 74 55 16 BsmI GAATG_Cn' Cuts at: 0 606 1788 3731 5160 Size: 606 1182 1943 1429 Fragments arranged by size: 1943 1429 1182 606 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1766 5160 Size: 1766 3394 Bsp1286I G_dGCh'C Cuts at: 0 74 169 193 230 248 307 393 695 Size: 74 95 24 37 18 59 86 302 Cuts at: 695 725 783 1472 1587 1698 1955 2269 2471 Size: 30 58 689 115 111 257 314 202 Cuts at: 2471 3665 3800 3806 4196 4368 4475 4484 4624 Size: 1194 135 6 390 172 107 9 140 Cuts at: 4624 5144 5160 Size: 520 16 Fragments arranged by size: 1194 689 520 390 314 302 257 202 172 140 135 115 111 107 95 86 74 59 58 37 30 24 18 16 9 6 BspHI T'CATG_A Cuts at: 0 992 5160 Size: 992 4168 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 205 299 2344 2372 2504 2849 5160 Size: 205 94 2045 28 132 345 2311 Fragments arranged by size: 2311 2045 345 205 132 94 28 BsrBI CCG'CTC Cuts at: 0 2951 5160 Size: 2951 2209 BsrDI GCAATG_nn' Cuts at: 0 572 2625 3336 5160 Size: 572 2053 711 1824 Fragments arranged by size: 2053 1824 711 572 BsrFI r'CCGG_y Cuts at: 0 1616 2869 5160 Size: 1616 1253 2291 Fragments arranged by size: 2291 1616 1253 BsrGI T'GTAC_A Cuts at: 0 1346 5160 Size: 1346 3814 BssHII G'CGCG_C Cuts at: 0 1321 5160 Size: 1321 3839 BssSI C'ACGA_G Cuts at: 0 2736 5160 Size: 2736 2424 BstBI TT'CG_AA Cuts at: 0 1014 5160 Size: 1014 4146 BstXI CCAn_nnnn'nTGG Cuts at: 0 5058 5160 Size: 5058 102 BstYI r'GATC_y Cuts at: 0 1256 2300 2732 3180 4125 4520 4785 4982 Size: 1256 1044 432 448 945 395 265 197 Cuts at: 4982 5062 5160 Size: 80 98 Fragments arranged by size: 1256 1044 945 448 432 395 265 197 98 80 BtgI C'CryG_G Cuts at: 0 686 1376 1420 1484 1550 1577 4490 4644 Size: 686 690 44 64 66 27 2913 154 Cuts at: 4644 5160 Size: 516 Fragments arranged by size: 2913 690 686 516 154 66 64 44 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 4884 5160 Size: 4884 276 BtsI GCAGTG_nn' Cuts at: 0 618 1663 2286 2460 2800 2898 2930 3042 Size: 618 1045 623 174 340 98 32 112 Cuts at: 3042 5160 Size: 2118 Fragments arranged by size: 2118 1045 623 618 340 174 112 98 32 DraI TTT'AAA Cuts at: 0 4041 5160 Size: 4041 1119 DraIII CAC_nnn'GTG Cuts at: 0 3613 4200 4682 5160 Size: 3613 587 482 478 Fragments arranged by size: 3613 587 482 478 EaeI y'GGCC_r Cuts at: 0 705 966 1064 1553 1869 3119 3333 3416 Size: 705 261 98 489 316 1250 214 83 Cuts at: 3416 3761 5160 Size: 345 1399 Fragments arranged by size: 1399 1250 705 489 345 316 261 214 98 83 EagI C'GGCC_G Cuts at: 0 3416 5160 Size: 3416 1744 EarI CTCTTCn'nnn_ Cuts at: 0 62 280 586 1706 2356 3249 3532 3820 Size: 62 218 306 1120 650 893 283 288 Cuts at: 3820 4104 4178 4286 4385 5001 5160 Size: 284 74 108 99 616 159 Fragments arranged by size: 1120 893 650 616 306 288 284 283 218 159 108 99 74 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2259 5160 Size: 2259 2901 EcoICRI GAG'CTC Cuts at: 0 72 246 2267 4622 5142 5160 Size: 72 174 2021 2355 520 18 Fragments arranged by size: 2355 2021 520 174 72 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 86 195 1165 1282 1730 2047 2608 3189 Size: 86 109 970 117 448 317 561 581 Cuts at: 3189 3613 3844 3862 4229 5062 5160 Size: 424 231 18 367 833 98 Fragments arranged by size: 970 833 581 561 448 424 367 317 231 117 109 98 86 18 EcoO109I rG'GnC_Cy Cuts at: 0 166 389 390 524 2468 2919 3578 4416 Size: 166 223 1 134 1944 451 659 838 Cuts at: 4416 4480 4779 5160 Size: 64 299 381 Fragments arranged by size: 1944 838 659 451 381 299 223 166 134 64 1 EcoRI G'AATT_C Cuts at: 0 419 1370 5160 Size: 419 951 3790 Fragments arranged by size: 3790 951 419 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 4626 5160 Size: 4626 534 HaeII r_GCGC'y Cuts at: 0 612 1526 3263 3606 3770 4500 5160 Size: 612 914 1737 343 164 730 660 Fragments arranged by size: 1737 914 730 660 612 343 164 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 626 658 1664 1696 1881 1913 4405 4437 Size: 626 32 1006 32 185 32 2492 32 Cuts at: 4437 4606 4638 5065 5097 5160 Size: 169 32 427 32 63 Fragments arranged by size: 2492 1006 626 427 185 169 63 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 668 2066 4908 5160 Size: 668 1398 2842 252 Fragments arranged by size: 2842 1398 668 252 KasI G'GCGC_C Cuts at: 0 3259 5160 Size: 3259 1901 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2749 3359 4829 5160 Size: 2749 610 1470 331 Fragments arranged by size: 2749 1470 610 331 MscI TGG'CCA Cuts at: 0 707 968 1066 1555 1871 3121 3763 5160 Size: 707 261 98 489 316 1250 642 1397 Fragments arranged by size: 1397 1250 707 642 489 316 261 98 MslI CAynn'nnrTG Cuts at: 0 199 599 2407 2935 3158 3673 3859 5160 Size: 199 400 1808 528 223 515 186 1301 Fragments arranged by size: 1808 1301 528 515 400 223 199 186 MspA1I CmG'CkG Cuts at: 0 369 703 2040 2321 2781 3591 3948 4122 Size: 369 334 1337 281 460 810 357 174 Cuts at: 4122 5160 Size: 1038 Fragments arranged by size: 1337 1038 810 460 369 357 334 281 174 NaeI GCC'GGC Cuts at: 0 1618 5160 Size: 1618 3542 NarI GG'CG_CC Cuts at: 0 3260 5160 Size: 3260 1900 NcoI C'CATG_G Cuts at: 0 686 1376 1420 1484 4490 4644 5160 Size: 686 690 44 64 3006 154 516 Fragments arranged by size: 3006 690 686 516 154 64 44 NdeI CA'TA_TG Cuts at: 0 596 2514 5160 Size: 596 1918 2646 Fragments arranged by size: 2646 1918 596 NgoMIV G'CCGG_C Cuts at: 0 1616 5160 Size: 1616 3544 NotI GC'GGCC_GC Cuts at: 0 3416 5160 Size: 3416 1744 NsiI A_TGCA'T Cuts at: 0 2641 3975 5160 Size: 2641 1334 1185 Fragments arranged by size: 2641 1334 1185 NspI r_CATG'y Cuts at: 0 21 327 541 1347 1624 2639 2643 3973 Size: 21 306 214 806 277 1015 4 1330 Cuts at: 3973 4335 5160 Size: 362 825 Fragments arranged by size: 1330 1015 825 806 362 306 277 214 21 4 PasI CC'CwG_GG Cuts at: 0 528 726 1596 2834 3343 3726 4476 5160 Size: 528 198 870 1238 509 383 750 684 Fragments arranged by size: 1238 870 750 684 528 509 383 198 PciI A'CATG_T Cuts at: 0 537 1343 5160 Size: 537 806 3817 Fragments arranged by size: 3817 806 537 PflMI CCAn_nnn'nTGG Cuts at: 0 1878 2904 5160 Size: 1878 1026 2256 Fragments arranged by size: 2256 1878 1026 PfoI T'CCnGG_A Cuts at: 0 1259 1892 3896 4781 5160 Size: 1259 633 2004 885 379 Fragments arranged by size: 2004 1259 885 633 379 PpuMI rG'GwC_Cy Cuts at: 0 524 3578 4416 4779 5160 Size: 524 3054 838 363 381 Fragments arranged by size: 3054 838 524 381 363 PshAI GACnn'nnGTC Cuts at: 0 3434 3509 5160 Size: 3434 75 1651 Fragments arranged by size: 3434 1651 75 PspOMI G'GGCC_C Cuts at: 0 165 389 2467 4480 5160 Size: 165 224 2078 2013 680 Fragments arranged by size: 2078 2013 680 224 165 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 94 126 5160 Size: 94 32 5034 Fragments arranged by size: 5034 94 32 PstI C_TGCA'G Cuts at: 0 38 174 2369 2684 3098 3590 5160 Size: 38 136 2195 315 414 492 1570 Fragments arranged by size: 2195 1570 492 414 315 136 38 PvuII CAG'CTG Cuts at: 0 369 703 3591 3948 5160 Size: 369 334 2888 357 1212 Fragments arranged by size: 2888 1212 369 357 334 RsrII CG'GwC_CG Cuts at: 0 3018 5160 Size: 3018 2142 SacI G_AGCT'C Cuts at: 0 74 248 2269 4624 5144 5160 Size: 74 174 2021 2355 520 16 Fragments arranged by size: 2355 2021 520 174 74 16 SalI G'TCGA_C Cuts at: 0 4906 5160 Size: 4906 254 SanDI GG'GwC_CC Cuts at: 0 524 5160 Size: 524 4636 SapI GCTCTTCn'nnn_ Cuts at: 0 62 4104 5160 Size: 62 4042 1056 Fragments arranged by size: 4042 1056 62 SfcI C'TryA_G Cuts at: 0 34 170 1076 2365 2373 2680 3094 3507 Size: 34 136 906 1289 8 307 414 413 Cuts at: 3507 3586 5005 5160 Size: 79 1419 155 Fragments arranged by size: 1419 1289 906 414 413 307 155 136 79 34 8 SfoI GGC'GCC Cuts at: 0 3261 5160 Size: 3261 1899 SmaI CCC'GGG Cuts at: 0 340 1948 5160 Size: 340 1608 3212 Fragments arranged by size: 3212 1608 340 SmlI C'TyrA_G Cuts at: 0 73 412 918 3267 3313 5143 5160 Size: 73 339 506 2349 46 1830 17 Fragments arranged by size: 2349 1830 506 339 73 46 17 SphI G_CATG'C Cuts at: 0 2639 2643 5160 Size: 2639 4 2517 Fragments arranged by size: 2639 2517 4 SspI AAT'ATT Cuts at: 0 1197 5160 Size: 1197 3963 StuI AGG'CCT Cuts at: 0 1277 1750 1822 5132 5160 Size: 1277 473 72 3310 28 Fragments arranged by size: 3310 1277 473 72 28 StyI C'CwwG_G Cuts at: 0 686 942 1376 1420 1484 1862 2387 3736 Size: 686 256 434 44 64 378 525 1349 Cuts at: 3736 4490 4644 4667 5160 Size: 754 154 23 493 Fragments arranged by size: 1349 754 686 525 493 434 378 256 154 64 44 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 786 1086 1127 1487 2136 2473 3685 3798 Size: 786 300 41 360 649 337 1212 113 Cuts at: 3798 4474 5160 Size: 676 686 Fragments arranged by size: 1212 786 686 676 649 360 337 300 113 41 TatI w'GTAC_w Cuts at: 0 1126 1346 5160 Size: 1126 220 3814 Fragments arranged by size: 3814 1126 220 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1292 1324 1660 1692 3140 3172 4552 4584 Size: 1292 32 336 32 1448 32 1380 32 Cuts at: 4584 5160 Size: 576 Fragments arranged by size: 1448 1380 1292 576 336 32 32 32 32 XbaI T'CTAG_A Cuts at: 0 894 5160 Size: 894 4266 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1418 3864 5160 Size: 1418 2446 1296 Fragments arranged by size: 2446 1418 1296 XmaI C'CCGG_G Cuts at: 0 338 1946 5160 Size: 338 1608 3214 Fragments arranged by size: 3214 1608 338 XmnI GAAnn'nnTTC Cuts at: 0 3931 5160 Size: 3931 1229 ZraI GAC'GTC Cuts at: 0 3894 5160 Size: 3894 1266 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PsrI PstI PvuII RsrII SacI SalI SanDI SapI SfcI SfoI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AgeI AhdI AloI AscI AseI AsiSI BaeI BmtI BsiWI BspEI BstAPI BstEII BstZ17I Bsu36I ClaI CspCI DrdI EcoNI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NheI NruI PacI PmeI PmlI PpiI PsiI PspXI PvuI SacII SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I XhoI