################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 59 GACGCC G[GA]^CG[CT]C AlwI 443 GGATC GGATC(4/5) AspHI 228 GAGCAC G[AT]GC[AT]^C AvaII 501 GGACC G^G[AT]CC BbsI 147 GAAGAC GAAGAC(2/6) BfaI 26 CTAG C^TAG BpuAI 147 GAAGAC GAAGAC(2/6) BsaHI 59 GACGCC G[GA]^CG[CT]C BsgI 41 CTGCAC GTGCAG(16/14) BsiHKAI 228 GAGCAC G[AT]GC[AT]^C BsmAI 566 GAGAC GTCTC(1/5) BsmFI 495 GTCCC GGGAC(10/14) BspDI 583 ATCGAT AT^CGAT BsrI 569 ACTGG ACTGG(1/-1) BssHII 219 GCGCGC G^CGCGC BstNI 498 CCTGG CC^[AT]GG ClaI 583 ATCGAT AT^CGAT DraII 478 GGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 590 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 89 CCACGG C^C[GA][CT]GG EaeI 254 TGGCCG [CT]^GGCC[GA] EarI 325 GAAGAG CTCTTC(1/4) EcoNI 516 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 478 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRII 498 CCTGG ^CC[AT]GG HaeII 468 GGCGCT [GA]GCGC^[CT] HgaI 59 GACGC GACGC(5/10) Hin1I 59 GACGCC G[GA]^CG[CT]C Ksp632I 325 GAAGAG CTCTTC(1/4) MaeI 26 CTAG C^TAG MaeIII 352 GTCAC ^GT[ACGT]AC MseI 416 TTAA T^TAA MslI 301 CACGCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 498 CCTGG CC^[AT]GG NlaIII 459 CATG CATG^ PaeR7I 129 CTCGAG C^TCGAG PflMI 240 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 142 CAGCTG CAG^CTG SmaI 482 CCCGGG CCC^GGG Tru9I 416 TTAA T^TAA Tsp45I 352 GTCAC ^GT[GC]AC Tsp509I 137 AATT ^AATT TspRI 590 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 240 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 129 CTCGAG C^TCGAG XmaCI 482 CCCGGG C^CCGGG XmaI 482 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwNI 241 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG 466 CAGGCGCTG ApaI 479 GGGCCC GGGCC^C 509 GGGCCC AvaI 129 CTCGAG C^[CT]CG[GA]G 482 CCCGGG BanII 479 GGGCCC G[GA]GC[CT]^C 509 GGGCCC BcgI 131 CGAGAAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 575 GCAAGAACATCG BpmI 181 CTGGAG CTGGAG(16/14) 247 CTGGAG BseRI 66 CTCCTC GAGGAG(10/8) 196 GAGGAG BsoBI 129 CTCGAG C^[CT]CG[GA]G 482 CCCGGG BspMI 503 ACCTGC ACCTGC(4/8) 522 GCAGGT BsrBI 63 CCGCTC CCGCTC(-3/-3) 199 GAGCGG BstAPI 230 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 465 GCAGGCGCTGC BstF5I 6 GGATG GGATG(2/0) 321 GGATG FauI 233 CCCGC CCCGC(4/6) 507 GCGGG FokI 6 GGATG GGATG(9/13) 321 GGATG HphI 525 GGTGA GGTGA(8/7) 353 TCACC SmlI 129 CTCGAG C^T[CT][GA]AG 517 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AccII AciI AluI BbvI BcnI BglII BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MflI MnlI MspA1I MspI MvnI MwoI NciI NdeII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI TthHB8I XhoII