(Circular) (Six-base) MAPSORT of: pFN21AE5284.seq Check: 9347 from: 1 to: 5335 pFN21AE5284 5335 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2518 2518 Size: 5335 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3145 278 Size: 53 83 186 936 1609 2468 Fragments arranged by size: 2468 1609 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2678 1937 Size: 741 4594 Acc65I G'GTAC_C Cuts at: 2656 2656 Size: 5335 AclI AA'CG_TT Cuts at: 1563 3462 3835 1563 Size: 1899 373 3063 Fragments arranged by size: 3063 1899 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 3398 4782 5050 1632 Size: 539 1227 1384 268 1917 Fragments arranged by size: 1917 1384 1227 539 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5138 AflIII A'CryG_T Cuts at: 1203 4234 5211 1203 Size: 3031 977 1327 Fragments arranged by size: 3031 1327 977 AgeI A'CCGG_T Cuts at: 4166 4166 Size: 5335 AhdI GACnn_n'nnGTC Cuts at: 4064 4064 Size: 5335 AleI CACnn'nnGTG Cuts at: 1166 2310 1166 Size: 1144 4191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5335 AlwNI CAG_nnn'CTG Cuts at: 2251 2476 3078 4650 2251 Size: 225 602 1572 2936 Fragments arranged by size: 2936 1572 602 225 ApaI G_GGCC'C Cuts at: 2488 2518 2488 Size: 30 5305 ApaLI G'TGCA_C Cuts at: 3392 4548 3392 Size: 1156 4179 ApoI r'AATT_y Cuts at: 1493 2644 2870 3022 5131 1493 Size: 1151 226 152 2109 1697 Fragments arranged by size: 2109 1697 1151 226 152 AseI AT'TA_AT Cuts at: 160 1032 3889 160 Size: 872 2857 1606 Fragments arranged by size: 2857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 AvaI C'yCGr_G Cuts at: 1870 1943 2134 2487 2660 1870 Size: 73 191 353 173 4545 Fragments arranged by size: 4545 353 191 173 73 BamHI G'GATC_C Cuts at: 2665 3032 2665 Size: 367 4968 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2656 4111 618 Size: 325 217 627 869 1455 1842 Fragments arranged by size: 1842 1455 869 627 325 217 BanII G_rGCy'C Cuts at: 1391 2488 2518 2654 1391 Size: 1097 30 136 4072 Fragments arranged by size: 4072 1097 136 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2159 928 Size: 971 260 4104 Fragments arranged by size: 4104 971 260 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2125 2159 2569 2603 3526 3560 1903 Size: 34 188 34 410 34 923 34 3678 Fragments arranged by size: 3678 923 410 188 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3227 4437 3227 Size: 1210 4125 BclI T'GATC_A Cuts at: 1548 1548 Size: 5335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3946 136 Size: 107 122 71 631 605 2274 1525 Fragments arranged by size: 2274 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2124 2383 2397 5330 2124 Size: 259 14 2933 2129 Fragments arranged by size: 2933 2129 259 14 BlpI GC'TnA_GC Cuts at: 2752 2752 Size: 5335 Bme1580I G_kGCm'C Cuts at: 1790 2488 2518 3396 4552 1790 Size: 698 30 878 1156 2573 Fragments arranged by size: 2573 1156 878 698 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3066 4024 442 Size: 416 53 83 399 24 1649 958 1753 Fragments arranged by size: 1753 1649 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2273 3995 1239 Size: 968 66 1722 2579 Fragments arranged by size: 2579 1722 968 66 Bpu10I CC'TnA_GC Cuts at: 2493 2493 Size: 5335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2507 3460 4325 4623 2507 Size: 953 865 298 3219 Fragments arranged by size: 3219 953 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3998 882 Size: 617 2499 2219 Fragments arranged by size: 2499 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3126 493 Size: 711 1922 2702 Fragments arranged by size: 2702 1922 711 BsaBI GATnn'nnATC Cuts at: 5329 5329 Size: 5335 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2065 3142 3524 4159 Size: 53 83 186 936 532 1077 382 635 Cuts at: 4159 275 Size: 1451 Fragments arranged by size: 1451 1077 936 635 532 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3768 4166 4440 4587 4998 3768 Size: 398 274 147 411 4105 Fragments arranged by size: 4105 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5110 Fragments arranged by size: 5110 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2062 2216 1186 Size: 876 154 4305 Fragments arranged by size: 4305 876 154 BseYI C'CCAG_C Cuts at: 2266 2479 4538 5034 2266 Size: 213 2059 496 2567 Fragments arranged by size: 2567 2059 496 213 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2031 1163 Size: 573 295 4467 Fragments arranged by size: 4467 573 295 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2785 3546 4574 1540 Size: 275 186 784 761 1028 2301 Fragments arranged by size: 2301 1028 784 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2237 3396 3481 4552 2237 Size: 1159 85 1071 3020 Fragments arranged by size: 3020 1159 1071 85 BsmI GAATG_Cn' Cuts at: 2846 2939 2846 Size: 93 5242 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2488 2518 2654 3396 3481 4552 Size: 399 447 251 30 136 742 85 1071 Cuts at: 4552 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 447 399 251 136 85 30 BspEI T'CCGG_A Cuts at: 4998 4998 Size: 5335 BspHI T'CATG_A Cuts at: 3224 4954 3224 Size: 1730 3605 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 2518 2690 844 Size: 1673 1 172 3489 Fragments arranged by size: 3489 1673 172 1 BsrBI CCG'CTC Cuts at: 1394 1823 2070 2206 3222 1394 Size: 429 247 136 1016 3507 Fragments arranged by size: 3507 1016 429 247 136 BsrDI GCAATG_nn' Cuts at: 65 1248 3830 4004 65 Size: 1183 2582 174 1396 Fragments arranged by size: 2582 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3979 4166 1700 Size: 110 2169 187 2869 Fragments arranged by size: 2869 2169 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5335 BssHII G'CGCG_C Cuts at: 1925 2224 1925 Size: 299 5036 BssSI C'ACGA_G Cuts at: 3395 4407 3395 Size: 1012 4323 BstAPI GCAn_nnn'nTGC Cuts at: 794 2241 2476 794 Size: 1447 235 3653 Fragments arranged by size: 3653 1447 235 BstBI TT'CG_AA Cuts at: 4139 4139 Size: 5335 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5335 BstYI r'GATC_y Cuts at: 1972 2124 2383 2397 2448 2665 3032 3417 3434 Size: 152 259 14 51 217 367 385 17 Cuts at: 3434 4886 4972 4984 5330 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 259 217 152 86 51 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 513 Size: 552 939 90 3754 Fragments arranged by size: 3754 939 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2037 487 Size: 683 56 811 3785 Fragments arranged by size: 3785 811 683 56 BtsI GCAGTG_nn' Cuts at: 2851 3645 3665 2851 Size: 794 20 4521 Fragments arranged by size: 4521 794 20 ClaI AT'CG_AT Cuts at: 1554 2589 1554 Size: 1035 4300 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5300 DraI TTT'AAA Cuts at: 2639 2639 Size: 5335 DraIII CAC_nnn'GTG Cuts at: 2600 2600 Size: 5335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4342 809 Size: 507 3026 1802 Fragments arranged by size: 3026 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2782 3671 Size: 54 1425 50 275 18 429 523 889 Cuts at: 3671 5300 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 523 429 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2782 1537 Size: 275 970 4090 Fragments arranged by size: 4090 970 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 3265 1353 Size: 972 940 3423 Fragments arranged by size: 3423 972 940 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2119 2213 3922 4296 4442 Size: 1064 138 435 95 94 1709 374 146 Cuts at: 4442 387 Size: 1280 Fragments arranged by size: 1709 1280 1064 435 374 146 138 95 94 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2207 2273 3398 3995 4782 5050 Size: 393 539 36 66 1125 597 787 268 Cuts at: 5050 1239 Size: 1524 Fragments arranged by size: 1524 1125 787 597 539 393 268 66 36 EcoNI CCTnn'n_nnAGG Cuts at: 2525 2525 Size: 5335 EcoO109I rG'GnC_Cy Cuts at: 2484 2484 Size: 5335 EcoRI G'AATT_C Cuts at: 2644 3022 2644 Size: 378 4957 FspI TGC'GCA Cuts at: 3841 3841 Size: 5335 HaeII r_GCGC'y Cuts at: 1678 2477 4482 1678 Size: 799 2005 2531 Fragments arranged by size: 2531 2005 799 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2368 2400 2433 2465 3976 Size: 32 135 32 796 32 33 32 1511 Cuts at: 3976 4008 4050 4082 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1511 796 135 42 33 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2679 2925 669 Size: 988 281 741 246 3079 Fragments arranged by size: 3079 988 741 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5335 HpaI GTT'AAC Cuts at: 2925 2925 Size: 5335 KpnI G_GTAC'C Cuts at: 2660 2660 Size: 5335 MfeI C'AATT_G Cuts at: 2934 2934 Size: 5335 MluI A'CGCG_T Cuts at: 5211 5211 Size: 5335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2100 4449 4633 1305 Size: 795 2349 184 2007 Fragments arranged by size: 2349 2007 795 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3513 Fragments arranged by size: 3513 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2310 3293 3652 3811 518 Size: 648 1144 983 359 159 2042 Fragments arranged by size: 2042 1144 983 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2149 2241 2251 2751 3428 4576 4821 1606 Size: 543 92 10 500 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 543 500 245 92 10 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3844 Fragments arranged by size: 3844 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5335 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5335 NotI GC'GGCC_GC Cuts at: 2782 2782 Size: 5335 NspI r_CATG'y Cuts at: 1132 1229 2693 4238 1132 Size: 97 1464 1545 2229 Fragments arranged by size: 2229 1545 1464 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5335 PciI A'CATG_T Cuts at: 4234 4234 Size: 5335 PflMI CCAn_nnn'nTGG Cuts at: 1108 2251 1108 Size: 1143 4192 PmeI GTTT'AAAC Cuts at: 2639 2639 Size: 5335 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3404 3436 4917 4949 3404 Size: 32 1481 32 3790 Fragments arranged by size: 3790 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5335 PsiI TTA'TAA Cuts at: 2905 2905 Size: 5335 PspOMI G'GGCC_C Cuts at: 2484 2514 2484 Size: 30 5305 PstI C_TGCA'G Cuts at: 830 2169 2286 2458 2687 830 Size: 1339 117 172 229 3478 Fragments arranged by size: 3478 1339 229 172 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5335 PvuII CAG'CTG Cuts at: 2149 2149 Size: 5335 SalI G'TCGA_C Cuts at: 1936 2677 1936 Size: 741 4594 SbfI CC_TGCA'GG Cuts at: 2687 2687 Size: 5335 ScaI AGT'ACT Cuts at: 3583 5295 3583 Size: 1712 3623 SfcI C'TryA_G Cuts at: 826 1045 2165 2282 2454 2683 3818 4499 4690 Size: 219 1120 117 172 229 1135 681 191 Cuts at: 4690 826 Size: 1471 Fragments arranged by size: 1471 1135 1120 681 229 219 191 172 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5335 SmaI CCC'GGG Cuts at: 1872 2489 2662 1872 Size: 617 173 4545 Fragments arranged by size: 4545 617 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 2134 2522 3439 4340 4602 820 Size: 197 926 191 388 917 901 262 1553 Fragments arranged by size: 1553 926 917 901 388 262 197 191 SnaBI TAC'GTA Cuts at: 493 3126 493 Size: 2633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5335 SphI G_CATG'C Cuts at: 1132 2693 1132 Size: 1561 3774 SrfI GCCC'GGGC Cuts at: 2489 2489 Size: 5335 SspI AAT'ATT Cuts at: 5 52 3259 5 Size: 47 3207 2081 Fragments arranged by size: 3207 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5335 StyI C'CwwG_G Cuts at: 513 1065 2004 2774 513 Size: 552 939 770 3074 Fragments arranged by size: 3074 939 770 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3362 3379 3532 3717 4162 325 Size: 1255 1782 17 153 185 445 1498 Fragments arranged by size: 1782 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3581 5293 96 Size: 274 80 33 51 1443 1604 1712 138 Fragments arranged by size: 1712 1604 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5295 5327 5295 Size: 32 5303 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5335 XbaI T'CTAG_A Cuts at: 2671 2671 Size: 5335 XhoI C'TCGA_G Cuts at: 1943 2134 1943 Size: 191 5144 XmaI C'CCGG_G Cuts at: 1870 2487 2660 1870 Size: 617 173 4545 Fragments arranged by size: 4545 617 173 XmnI GAAnn'nnTTC Cuts at: 3464 3464 Size: 5335 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3143 276 Size: 53 83 186 936 1609 2468 Fragments arranged by size: 2468 1609 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI