################################## # NO CUT ENZYMES ( 201 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FseI FspI HaeII HaeIII HhaI HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 63 GACGTC GACGT^C AcyI 63 GACGTC G[GA]^CG[CT]C AfaI 131 GTAC GT^AC AspHI 213 GAGCTC G[AT]GC[AT]^C BbsI 312 GAAGAC GAAGAC(2/6) BpuAI 312 GAAGAC GAAGAC(2/6) BsaHI 63 GACGTC G[GA]^CG[CT]C BsaJI 218 CCAAGG C^C[ACGT][ACGT]GG BsiEI 102 CGGTCG CG[GA][CT]^CG BsiHKAI 213 GAGCTC G[AT]GC[AT]^C BsmAI 290 GAGAC GTCTC(1/5) BspMI 348 ACCTGC ACCTGC(4/8) BstAPI 343 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 273 GGATG GGATG(2/0) DpnII 198 GATC ^GATC EarI 285 GAAGAG CTCTTC(1/4) EcoT14I 218 CCAAGG C^C[AT][AT]GG FauI 151 CCCGC CCCGC(4/6) FokI 273 GGATG GGATG(9/13) HapII 60 CCGG C^CGG HgaI 74 GCGTC GACGC(5/10) Hin1I 63 GACGTC G[GA]^CG[CT]C HpaII 60 CCGG C^CGG Ksp632I 285 GAAGAG CTCTTC(1/4) MaeII 64 ACGT A^CGT MboI 198 GATC ^GATC MseI 172 TTAA T^TAA MslI 204 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 60 CCGG C^CGG NdeII 198 GATC ^GATC NlaIII 270 CATG CATG^ RsaI 131 GTAC GT^AC SacI 213 GAGCTC GAGCT^C Sau3AI 198 GATC ^GATC StyI 218 CCAAGG C^C[AT][AT]GG TaqI 105 TCGA T^CGA Tru9I 172 TTAA T^TAA TspRI 204 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 105 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AciI 152 CCGC CCGC(-3/-1) 353 CCGC AlwNI 22 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 344 CAGCACCTG BanII 148 GAGCCC G[GA]GC[CT]^C 213 GAGCTC BmyI 148 GAGCCC G[AGT]GC[ACT]^C 213 GAGCTC BsiYI 60 CCGGACGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 121 CCAAAGAAGGG BslI 60 CCGGACGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 121 CCAAAGAAGGG Bsp1286I 148 GAGCCC G[AGT]GC[ACT]^C 213 GAGCTC Cac8I 17 GCGAGC GC[ACGT]^[ACGT]GC 150 GCCCGC DdeI 259 CTTAG C^T[ACGT]AG 308 CTAAG HphI 210 GGTGA GGTGA(8/7) 83 TCACC MnlI 255 CCTC CCTC(7/6) 139 GAGG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AluI BbvI Fnu4HI ItaI MboII MwoI TseI