Restriction map for
pFN21AE5295
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BciVI BclI BcnI
BfrI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NarI
NciI NcoI NdeI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 48 CGCG CG^CG
AocI 193 CCTGAGG CC^T[ACGT]AGG
Asp700I 150 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 377 GTGCTC G[AT]GC[AT]^C
AvaI 392 CTCGGG C^[CT]CG[GA]G
BamHI 509 GGATCC G^GATCC
BanII 387 GGGCTC G[GA]GC[CT]^C
BcgI 38 GCAAGTATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 177 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 186 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 186 GCTAAGC GC^T[ACGT]AGC
BsiHKAI 377 GTGCTC G[AT]GC[AT]^C
BsoBI 392 CTCGGG C^[CT]CG[GA]G
BspMI 486 GCAGGT ACCTGC(4/8)
BsrFI 10 GCCGGC [GA]^CCGG[CT]
BstF5I 218 CATCC GGATG(2/0)
BstUI 48 CGCG CG^CG
BstYI 509 GGATCC [GA]^GATC[CT]
Bsu36I 193 CCTGAGG CC^T[ACGT]AGG
CelII 186 GCTAAGC GC^T[ACGT]AGC
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
DpnII 510 GATC ^GATC
Eco81I 193 CCTGAGG CC^T[ACGT]AGG
EcoNI 323 CCTTGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 218 CATCC GGATG(9/13)
HaeII 573 GGCGCT [GA]GCGC^[CT]
HgaI 49 GCGTC GACGC(5/10)
HincII 51 GTCAAC GT[CT]^[GA]AC
HindII 51 GTCAAC GT[CT]^[GA]AC
MaeII 264 ACGT A^CGT
MboI 510 GATC ^GATC
MflI 509 GGATCC [GA]^GATC[CT]
MvnI 48 CGCG CG^CG
NaeI 10 GCCGGC GCC^GGC
NdeII 510 GATC ^GATC
NgoMIV 10 GCCGGC G^CCGGC
NheI 558 GCTAGC G^CTAGC
PstI 499 CTGCAG CTGCA^G
SapI 493 GAAGAGC GCTCTTC(1/4)
Sau3AI 510 GATC ^GATC
SfaNI 412 GCATC GCATC(5/9)
SfcI 499 CTGCAG C^T[GA][CT]AG
SmlI 324 CTTGAG C^T[CT][GA]AG
Tsp45I 211 GTCAC ^GT[GC]AC
XhoII 509 GGATCC [GA]^GATC[CT]
XmnI 150 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 510 GATCC GGATC(4/5)
509 GGATC
AvaII 71 GGACC G^G[AT]CC
123 GGTCC
BsmAI 31 GAGAC GTCTC(1/5)
358 GAGAC
BsrI 127 CCAGT ACTGG(1/-1)
374 CCAGT
CfoI 78 GCGC GCG^C
574 GCGC
Cfr13I 71 GGACC G^G[ACGT]CC
123 GGTCC
DraII 70 GGGACCT [GA]G^G[ACGT]CC[CT]
122 AGGTCCC
EcoO109I 70 GGGACCT [GA]G^G[ACGT]CC[CT]
122 AGGTCCC
HapII 11 CCGG C^CGG
483 CCGG
HhaI 78 GCGC GCG^C
574 GCGC
HinP1I 78 GCGC G^CGC
574 GCGC
HinfI 333 GACTC G^A[ACGT]TC
427 GACTC
HpaII 11 CCGG C^CGG
483 CCGG
MaeIII 211 GTCAC ^GT[ACGT]AC
266 GTAAC
MspI 11 CCGG C^CGG
483 CCGG
PleI 333 GACTC GAGTC(4/5)
427 GACTC
PpuMI 70 GGGACCT [GA]G^G[AT]CC[CT]
122 AGGTCCC
PvuII 407 CAGCTG CAG^CTG
431 CAGCTG
Sau96I 71 GGACC G^G[ACGT]CC
123 GGTCC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BbvI BfaI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I DdeI
EarI EcoRII Fnu4HI HaeIII HphI
ItaI Ksp632I MaeI MboII MnlI
MspA1I MvaI MwoI NlaIV ScrFI
StuI TseI TspRI