(Circular) (Six-base) MAPSORT of: pFN21AE5295.seq Check: 938 from: 1 to: 5317 pFN21AE5295 5317 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2482 2482 Size: 5317 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3127 278 Size: 53 83 186 936 1591 2468 Fragments arranged by size: 2468 1591 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2660 1937 Size: 723 4594 Acc65I G'GTAC_C Cuts at: 2638 2638 Size: 5317 AclI AA'CG_TT Cuts at: 1563 3444 3817 1563 Size: 1881 373 3063 Fragments arranged by size: 3063 1881 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2253 2297 3380 4764 5032 1632 Size: 621 44 1083 1384 268 1917 Fragments arranged by size: 1917 1384 1083 621 268 44 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5120 AflIII A'CryG_T Cuts at: 1203 4216 5193 1203 Size: 3013 977 1327 Fragments arranged by size: 3013 1327 977 AgeI A'CCGG_T Cuts at: 4148 4148 Size: 5317 AhdI GACnn_n'nnGTC Cuts at: 4046 4046 Size: 5317 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5317 AlwNI CAG_nnn'CTG Cuts at: 3060 4632 3060 Size: 1572 3745 ApaLI G'TGCA_C Cuts at: 3374 4530 3374 Size: 1156 4161 ApoI r'AATT_y Cuts at: 1493 2626 2852 3004 5113 1493 Size: 1133 226 152 2109 1697 Fragments arranged by size: 2109 1697 1133 226 152 AseI AT'TA_AT Cuts at: 160 1032 3871 160 Size: 872 2839 1606 Fragments arranged by size: 2839 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5317 AvaI C'yCGr_G Cuts at: 1870 1943 2397 2642 1870 Size: 73 454 245 4545 Fragments arranged by size: 4545 454 245 73 BamHI G'GATC_C Cuts at: 2514 2647 3014 2514 Size: 133 367 4817 Fragments arranged by size: 4817 367 133 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2638 4093 618 Size: 325 217 627 851 1455 1842 Fragments arranged by size: 1842 1455 851 627 325 217 BanII G_rGCy'C Cuts at: 1391 2396 2636 1391 Size: 1005 240 4072 Fragments arranged by size: 4072 1005 240 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4346 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2032 2066 3508 3542 1903 Size: 34 95 34 1442 34 3678 Fragments arranged by size: 3678 1442 95 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3209 4419 3209 Size: 1210 4107 BclI T'GATC_A Cuts at: 1548 1548 Size: 5317 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2188 3928 136 Size: 107 122 71 631 605 516 1740 1525 Fragments arranged by size: 1740 1525 631 605 516 122 107 71 BglII A'GATC_T Cuts at: 5312 5312 Size: 5317 BlpI GC'TnA_GC Cuts at: 2192 2734 2192 Size: 542 4775 Bme1580I G_kGCm'C Cuts at: 1790 2118 3378 4534 1790 Size: 328 1260 1156 2573 Fragments arranged by size: 2573 1260 1156 328 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5317 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2126 2373 3048 Size: 416 53 83 399 24 709 247 675 Cuts at: 3048 4006 442 Size: 958 1753 Fragments arranged by size: 1753 958 709 675 416 399 247 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2567 1056 Size: 1511 3806 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2355 2355 Size: 5317 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3977 1239 Size: 2738 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2350 3442 4307 4605 2350 Size: 1092 865 298 3062 Fragments arranged by size: 3062 1092 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3980 882 Size: 617 2481 2219 Fragments arranged by size: 2481 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3108 493 Size: 711 1904 2702 Fragments arranged by size: 2702 1904 711 BsaBI GATnn'nnATC Cuts at: 5311 5311 Size: 5317 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3124 3506 4141 275 Size: 53 83 186 936 1591 382 635 1451 Fragments arranged by size: 1591 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3750 4148 4422 4569 4980 3750 Size: 398 274 147 411 4087 Fragments arranged by size: 4087 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5092 Fragments arranged by size: 5092 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2587 2603 1186 Size: 952 449 16 3900 Fragments arranged by size: 3900 952 449 16 BseYI C'CCAG_C Cuts at: 2522 2540 4520 5016 2522 Size: 18 1980 496 2823 Fragments arranged by size: 2823 1980 496 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4744 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2767 3528 4556 1540 Size: 275 186 766 761 1028 2301 Fragments arranged by size: 2301 1028 766 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2386 3378 3463 4534 2386 Size: 992 85 1071 3169 Fragments arranged by size: 3169 1071 992 85 BsmI GAATG_Cn' Cuts at: 2828 2921 2828 Size: 93 5224 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2386 2396 2636 3378 3463 4534 Size: 399 328 268 10 240 742 85 1071 Cuts at: 4534 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 328 268 240 85 10 BspEI T'CCGG_A Cuts at: 4980 4980 Size: 5317 BspHI T'CATG_A Cuts at: 3206 4936 3206 Size: 1730 3587 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2482 2672 844 Size: 1638 190 3489 Fragments arranged by size: 3489 1638 190 BsrBI CCG'CTC Cuts at: 1394 1823 3204 1394 Size: 429 1381 3507 Fragments arranged by size: 3507 1381 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3812 3986 65 Size: 1183 2564 174 1396 Fragments arranged by size: 2564 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 3961 4148 1700 Size: 110 205 1946 187 2869 Fragments arranged by size: 2869 1946 205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5317 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5317 BssSI C'ACGA_G Cuts at: 3377 4389 3377 Size: 1012 4305 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5317 BstBI TT'CG_AA Cuts at: 4121 4121 Size: 5317 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5317 BstYI r'GATC_y Cuts at: 1972 2514 2647 3014 3399 3416 4868 4954 4966 Size: 542 133 367 385 17 1452 86 12 Cuts at: 4966 5312 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 542 385 367 346 133 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2199 2199 Size: 5317 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3826 Fragments arranged by size: 3826 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4578 Fragments arranged by size: 4578 683 56 BtsI GCAGTG_nn' Cuts at: 2205 2833 3627 3647 2205 Size: 628 794 20 3875 Fragments arranged by size: 3875 794 628 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5317 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5282 DraI TTT'AAA Cuts at: 2621 2621 Size: 5317 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4324 809 Size: 507 3008 1802 Fragments arranged by size: 3008 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2764 3653 5282 Size: 54 1425 50 275 18 934 889 1629 Cuts at: 5282 8 Size: 43 Fragments arranged by size: 1629 1425 934 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2764 1537 Size: 275 952 4090 Fragments arranged by size: 4090 952 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2285 2355 2493 2580 3247 1353 Size: 932 70 138 87 667 3423 Fragments arranged by size: 3423 932 667 138 87 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3904 4278 4424 387 Size: 1064 138 2315 374 146 1280 Fragments arranged by size: 2315 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2253 2297 3380 3977 4764 5032 1239 Size: 393 621 44 1083 597 787 268 1524 Fragments arranged by size: 1524 1083 787 621 597 393 268 44 EcoNI CCTnn'n_nnAGG Cuts at: 2332 2332 Size: 5317 EcoO109I rG'GnC_Cy Cuts at: 2076 2128 2076 Size: 52 5265 EcoRI G'AATT_C Cuts at: 2626 3004 2626 Size: 378 4939 FspI TGC'GCA Cuts at: 3823 3823 Size: 5317 HaeII r_GCGC'y Cuts at: 1678 2582 4464 1678 Size: 904 1882 2531 Fragments arranged by size: 2531 1882 904 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2336 2368 3958 3990 4032 Size: 32 135 32 764 32 1590 32 42 Cuts at: 4032 4064 1373 Size: 32 2626 Fragments arranged by size: 2626 1590 764 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2058 2661 2907 669 Size: 988 281 120 603 246 3079 Fragments arranged by size: 3079 988 603 281 246 120 HindIII A'AGCT_T Cuts at: 748 748 Size: 5317 HpaI GTT'AAC Cuts at: 2907 2907 Size: 5317 KpnI G_GTAC'C Cuts at: 2642 2642 Size: 5317 MfeI C'AATT_G Cuts at: 2916 2916 Size: 5317 MluI A'CGCG_T Cuts at: 5193 5193 Size: 5317 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4431 4615 1305 Size: 3126 184 2007 Fragments arranged by size: 3126 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3495 Fragments arranged by size: 3495 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3275 3634 3793 518 Size: 648 2109 359 159 2042 Fragments arranged by size: 2109 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2414 2438 2512 2733 3410 4558 4803 Size: 486 322 24 74 221 677 1148 245 Cuts at: 4803 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 486 322 245 221 74 24 NaeI GCC'GGC Cuts at: 2017 2017 Size: 5317 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3826 Fragments arranged by size: 3826 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5317 NgoMIV G'CCGG_C Cuts at: 2015 2015 Size: 5317 NheI G'CTAG_C Cuts at: 1052 2563 1052 Size: 1511 3806 NotI GC'GGCC_GC Cuts at: 2764 2764 Size: 5317 NspI r_CATG'y Cuts at: 1132 1229 2675 4220 1132 Size: 97 1446 1545 2229 Fragments arranged by size: 2229 1545 1446 97 PasI CC'CwG_GG Cuts at: 1348 2186 1348 Size: 838 4479 PciI A'CATG_T Cuts at: 4216 4216 Size: 5317 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5317 PmeI GTTT'AAAC Cuts at: 2621 2621 Size: 5317 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2235 2267 3386 3418 4899 4931 2235 Size: 32 1119 32 1481 32 2621 Fragments arranged by size: 2621 1481 1119 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2076 2128 2076 Size: 52 5265 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5317 PsiI TTA'TAA Cuts at: 2887 2887 Size: 5317 PstI C_TGCA'G Cuts at: 830 2508 2669 830 Size: 1678 161 3478 Fragments arranged by size: 3478 1678 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5317 PvuII CAG'CTG Cuts at: 2414 2438 2414 Size: 24 5293 SalI G'TCGA_C Cuts at: 1936 2659 1936 Size: 723 4594 SapI GCTCTTCn'nnn_ Cuts at: 2493 2493 Size: 5317 SbfI CC_TGCA'GG Cuts at: 2669 2669 Size: 5317 ScaI AGT'ACT Cuts at: 3565 5277 3565 Size: 1712 3605 SfcI C'TryA_G Cuts at: 826 1045 2504 2665 3800 4481 4672 826 Size: 219 1459 161 1135 681 191 1471 Fragments arranged by size: 1471 1459 1135 681 219 191 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5317 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5317 SmaI CCC'GGG Cuts at: 1872 2644 1872 Size: 772 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2329 3421 4322 4584 820 Size: 197 926 386 1092 901 262 1553 Fragments arranged by size: 1553 1092 926 901 386 262 197 SnaBI TAC'GTA Cuts at: 493 3108 493 Size: 2615 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5317 SphI G_CATG'C Cuts at: 1132 2675 1132 Size: 1543 3774 SspI AAT'ATT Cuts at: 5 52 3241 5 Size: 47 3189 2081 Fragments arranged by size: 3189 2081 47 StuI AGG'CCT Cuts at: 1517 2230 2250 2327 2613 1517 Size: 713 20 77 286 4221 Fragments arranged by size: 4221 713 286 77 20 StyI C'CwwG_G Cuts at: 513 1065 2004 2756 513 Size: 552 939 752 3074 Fragments arranged by size: 3074 939 752 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3344 3361 3514 3699 4144 325 Size: 1255 1764 17 153 185 445 1498 Fragments arranged by size: 1764 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3563 5275 96 Size: 274 80 33 51 1443 1586 1712 138 Fragments arranged by size: 1712 1586 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5277 5309 5277 Size: 32 5285 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5317 XbaI T'CTAG_A Cuts at: 2653 2653 Size: 5317 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5317 XmaI C'CCGG_G Cuts at: 1870 2642 1870 Size: 772 4545 XmnI GAAnn'nnTTC Cuts at: 2159 3446 2159 Size: 1287 4030 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3125 276 Size: 53 83 186 936 1591 2468 Fragments arranged by size: 2468 1591 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI