################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Cac8I CfoI Cfr10I ClaI CpoI DraI DraII DraIII EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 294 CGCG CG^CG AfaI 139 GTAC GT^AC AhdI 113 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 756 GGATC GGATC(4/5) AocI 115 CCTAAGG CC^T[ACGT]AGG Asp700I 453 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 113 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 664 GAGCTC G[AT]GC[AT]^C AvaII 120 GGTCC G^G[AT]CC BciVI 386 GTATCC GTATCC(6/5) BcnI 669 CCGGG CC^[GC]GG BfaI 442 CTAG C^TAG BlpI 673 GCTCAGC GC^T[ACGT]AGC Bpu1102I 673 GCTCAGC GC^T[ACGT]AGC BsaI 219 GGTCTC GGTCTC(1/5) BsiHKAI 664 GAGCTC G[AT]GC[AT]^C BsmFI 398 GTCCC GGGAC(10/14) BspHI 627 TCATGA T^CATGA BspMI 342 GCAGGT ACCTGC(4/8) BstAPI 88 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 294 CGCG CG^CG Bsu36I 115 CCTAAGG CC^T[ACGT]AGG CelII 673 GCTCAGC GC^T[ACGT]AGC DpnII 757 GATC ^GATC DsaI 401 CCACGG C^C[GA][CT]GG Eam1105I 113 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 651 GAAGAG CTCTTC(1/4) Eco81I 115 CCTAAGG CC^T[ACGT]AGG EcoRI 125 GAATTC G^AATTC HapII 669 CCGG C^CGG HgaI 692 GCGTC GACGC(5/10) HpaII 669 CCGG C^CGG Ksp632I 651 GAAGAG CTCTTC(1/4) MaeI 442 CTAG C^TAG MaeII 134 ACGT A^CGT MboI 757 GATC ^GATC MboII 651 GAAGA GAAGA(8/7) MspI 669 CCGG C^CGG MvnI 294 CGCG CG^CG NciI 669 CCGGG CC^[GC]GG NdeII 757 GATC ^GATC RcaI 627 TCATGA T^CATGA RsaI 139 GTAC GT^AC SacI 664 GAGCTC GAGCT^C SapI 651 GAAGAGC GCTCTTC(1/4) Sau3AI 757 GATC ^GATC SfcI 527 CTGTAG C^T[GA][CT]AG SmlI 418 CTTGAG C^T[CT][GA]AG TaqI 305 TCGA T^CGA TfiI 624 GATTC G^A[AT]TC TthHB8I 305 TCGA T^CGA XmnI 453 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 293 CCGC CCGC(-3/-1) 591 CCGC AcsI 125 GAATTC [GA]^AATT[CT] 431 AAATTC ApoI 125 GAATTC [GA]^AATT[CT] 431 AAATTC BanII 664 GAGCTC G[GA]GC[CT]^C 671 GGGCTC BmyI 664 GAGCTC G[AGT]GC[ACT]^C 671 GGGCTC BsaJI 215 CCCAGG C^C[ACGT][ACGT]GG 401 CCACGG BsmAI 220 GTCTC GTCTC(1/5) 546 GTCTC Bsp1286I 664 GAGCTC G[AGT]GC[ACT]^C 671 GGGCTC BstF5I 600 CATCC GGATG(2/0) 657 CATCC BstNI 216 CCAGG CC^[AT]GG 603 CCAGG DrdI 348 GACTCCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 685 GACCTTAGCGTC EcoRII 216 CCAGG ^CC[AT]GG 603 CCAGG FokI 600 CATCC GGATG(9/13) 657 CATCC MseI 101 TTAA T^TAA 770 TTAA MvaI 216 CCAGG CC^[AT]GG 603 CCAGG PleI 348 GACTC GAGTC(4/5) 585 GAGTC Tru9I 101 TTAA T^TAA 770 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI BbvI BsiYI BslI BssKI Cfr13I DdeI Fnu4HI HaeIII HinfI HphI ItaI MaeIII MnlI MwoI NlaIII Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI