Restriction map for pFN21AE5334
 (Circular) (Six-base) MAPSORT of: pFN21AE5334.seq  Check: 5454  from: 1  to: 5488



pFN21AE5334  5488 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2338    2338 
   Size:       5488
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3298     278 
   Size:         53      83     186     936    1762    2468

  Fragments arranged by size: 

                2468    1762     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2831    1937 
   Size:        894    4594
 
Acc65I G'GTAC_C

Cuts at:   2809    2809 
   Size:       5488
 
AclI AA'CG_TT

Cuts at:   1563    3615    3988    1563 
   Size:       2052     373    3063

  Fragments arranged by size: 

                3063    2052     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3551    4935    5203    1632 
   Size:       1919    1384     268    1917

  Fragments arranged by size: 

                1919    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5291
 
AflIII A'CryG_T

Cuts at:   1203    4387    5364    1203 
   Size:       3184     977    1327

  Fragments arranged by size: 

                3184    1327     977
 
AgeI A'CCGG_T

Cuts at:   4319    4319 
   Size:       5488
 
AhdI GACnn_n'nnGTC

Cuts at:   2123    4217    2123 
   Size:       2094    3394
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5488
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5488
 
AlwNI CAG_nnn'CTG

Cuts at:   3231    4803    3231 
   Size:       1572    3916
 
ApaLI G'TGCA_C

Cuts at:   3545    4701    3545 
   Size:       1156    4332
 
ApoI r'AATT_y

Cuts at:   1493    2130    2436    2797    3023    3175    5284    1493 
   Size:        637     306     361     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     637     361     306     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4042     160 
   Size:        872    3010    1606

  Fragments arranged by size: 

                3010    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5488
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2813    1870 
   Size:         73     870    4545

  Fragments arranged by size: 

                4545     870      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2517    2550    2517 
   Size:         33    5455
 
BamHI G'GATC_C

Cuts at:   2818    3185    2818 
   Size:        367    5121
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2809    4264     618 
   Size:        325     217     627    1022    1455    1842

  Fragments arranged by size: 

                1842    1455    1022     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2673    2680    2807    1391 
   Size:       1282       7     127    4072

  Fragments arranged by size: 

                4072    1282     127       7
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4517
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3679    3713    1903 
   Size:         34    1742      34    3678

  Fragments arranged by size: 

                3678    1742      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2402    3380    4590    2402 
   Size:        978    1210    3300

  Fragments arranged by size: 

                3300    1210     978
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5488
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4099     136 
   Size:        107     122      71     631     605    2427    1525

  Fragments arranged by size: 

                2427    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5483    5483 
   Size:       5488
 
BlpI GC'TnA_GC

Cuts at:   2679    2905    2679 
   Size:        226    5262
 
Bme1580I G_kGCm'C

Cuts at:   1790    3549    4705    1790 
   Size:       1759    1156    2573

  Fragments arranged by size: 

                2573    1759    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5488
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3219    4177     442 
   Size:        416      53      83     399      24    1802     958    1753

  Fragments arranged by size: 

                1802    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5488
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4148    1239 
   Size:       2909    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2693    2693 
   Size:       5488
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2444    3613    4478    4776    2444 
   Size:       1169     865     298    3156

  Fragments arranged by size: 

                3156    1169     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2230    4151     882 
   Size:        617     731    1921    2219

  Fragments arranged by size: 

                2219    1921     731     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3279     493 
   Size:        711    2075    2702

  Fragments arranged by size: 

                2702    2075     711
 
BsaBI GATnn'nnATC

Cuts at:   5482    5482 
   Size:       5488
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3295    3677    4312     275 
   Size:         53      83     186     936    1762     382     635    1451

  Fragments arranged by size: 

                1762    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3921    4319    4593    4740    5151    3921 
   Size:        398     274     147     411    4258

  Fragments arranged by size: 

                4258     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2361    2391    1178 
   Size:         30     165      30     958      30    4275

  Fragments arranged by size: 

                4275     958     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5488
 
BseYI C'CCAG_C

Cuts at:   2573    4691    5187    2573 
   Size:       2118     496    2874

  Fragments arranged by size: 

                2874    2118     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4915
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2938    3699    4727    1540 
   Size:        275     186     937     761    1028    2301

  Fragments arranged by size: 

                2301    1028     937     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2673    3549    3634    4705    2673 
   Size:        876      85    1071    3456

  Fragments arranged by size: 

                3456    1071     876      85
 
BsmI GAATG_Cn'

Cuts at:   2999    3092    2999 
   Size:         93    5395
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2673    2680    2807    3549    3634    4705    1391 
   Size:        399     883       7     127     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     883     742     399     127      85       7
            
 
BspEI T'CCGG_A

Cuts at:   5151    5151 
   Size:       5488
 
BspHI T'CATG_A

Cuts at:   2632    3377    5107    2632 
   Size:        745    1730    3013

  Fragments arranged by size: 

                3013    1730     745
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2338    2843     844 
   Size:       1494     505    3489

  Fragments arranged by size: 

                3489    1494     505
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3375    1394 
   Size:        429    1552    3507

  Fragments arranged by size: 

                3507    1552     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3983    4157      65 
   Size:       1183    2735     174    1396

  Fragments arranged by size: 

                2735    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4132    4319    1700 
   Size:        110    2322     187    2869

  Fragments arranged by size: 

                2869    2322     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5488
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5488
 
BssSI C'ACGA_G

Cuts at:   3548    4560    3548 
   Size:       1012    4476
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2099     794 
   Size:       1305    4183
 
BstBI TT'CG_AA

Cuts at:   4292    4292 
   Size:       5488
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5488
 
BstYI r'GATC_y

Cuts at:   1972    2818    3185    3570    3587    5039    5125    5137    5483 
   Size:        846     367     385      17    1452      86      12     346

Cuts at:   5483    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     846     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2121    2121 
   Size:       5488
 
BtgI C'CryG_G

Cuts at:    513    1065    2406     513 
   Size:        552    1341    3595

  Fragments arranged by size: 

                3595    1341     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4749

  Fragments arranged by size: 

                4749     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2074    3004    3798    3818    2074 
   Size:        930     794      20    3744

  Fragments arranged by size: 

                3744     930     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5488
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5453
 
DraI TTT'AAA

Cuts at:   2792    2792 
   Size:       5488
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2359    2696    4495     809 
   Size:        507    1043     337    1799    1802

  Fragments arranged by size: 

                1802    1799    1043     507     337
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2935    3824    5453 
   Size:         54    1425      50     275      18    1105     889    1629

Cuts at:   5453       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1105     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2935    1537 
   Size:        275    1123    4090

  Fragments arranged by size: 

                4090    1123     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2651    3418    1353 
   Size:       1298     767    3423

  Fragments arranged by size: 

                3423    1298     767
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4075    4449    4595     387 
   Size:       1064     138    2486     374     146    1280

  Fragments arranged by size: 

                2486    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2671    2671 
   Size:       5488
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3551    4148    4935    5203    1239 
   Size:        393    1919     597     787     268    1524

  Fragments arranged by size: 

                1919    1524     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2130    2797    3175    2130 
   Size:        667     378    4443

  Fragments arranged by size: 

                4443     667     378
 
FspI TGC'GCA

Cuts at:   3994    3994 
   Size:       5488
 
HaeII r_GCGC'y

Cuts at:   1678    4635    1678 
   Size:       2957    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2109    2141    2325    2357    2361 
   Size:         32     135      32     537      32     184      32       4

Cuts at:   2361    2393    2710    2742    4129    4161    4203    4235    1373 
   Size:         32     317      32    1387      32      42      32    2626

  Fragments arranged by size: 

                2626    1387     537     317     184     135      42      32
                  32      32      32      32      32      32      32       4
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2832    3078     669 
   Size:        988     281     894     246    3079

  Fragments arranged by size: 

                3079     988     894     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5488
 
HpaI GTT'AAC

Cuts at:   3078    3078 
   Size:       5488
 
KpnI G_GTAC'C

Cuts at:   2813    2813 
   Size:       5488
 
MfeI C'AATT_G

Cuts at:   3087    3087 
   Size:       5488
 
MluI A'CGCG_T

Cuts at:   5364    5364 
   Size:       5488
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2347    4602    4786    1305 
   Size:       1042    2255     184    2007

  Fragments arranged by size: 

                2255    2007    1042     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3666

  Fragments arranged by size: 

                3666    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3446    3805    3964     518 
   Size:        648    2280     359     159    2042

  Fragments arranged by size: 

                2280    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2904    3581    4729    4974    1606 
   Size:       1298     677    1148     245    2120

  Fragments arranged by size: 

                2120    1298    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4936
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5488
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5488
 
NotI GC'GGCC_GC

Cuts at:   2935    2935 
   Size:       5488
 
NspI r_CATG'y

Cuts at:   1132    1229    2846    4391    1132 
   Size:         97    1617    1545    2229

  Fragments arranged by size: 

                2229    1617    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5488
 
PciI A'CATG_T

Cuts at:   4387    4387 
   Size:       5488
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5488
 
PfoI T'CCnGG_A

Cuts at:   2607    2607 
   Size:       5488
 
PmeI GTTT'AAAC

Cuts at:   2792    2792 
   Size:       5488
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3557    3589    5070    5102    3557 
   Size:         32    1481      32    3943

  Fragments arranged by size: 

                3943    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5488
 
PsiI TTA'TAA

Cuts at:   3058    3058 
   Size:       5488
 
PstI C_TGCA'G

Cuts at:    830    2840     830 
   Size:       2010    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5488
 
SacI G_AGCT'C

Cuts at:   2673    2673 
   Size:       5488
 
SalI G'TCGA_C

Cuts at:   1936    2830    1936 
   Size:        894    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2651    2651 
   Size:       5488
 
SbfI CC_TGCA'GG

Cuts at:   2840    2840 
   Size:       5488
 
ScaI AGT'ACT

Cuts at:   3736    5448    3736 
   Size:       1712    3776
 
SfcI C'TryA_G

Cuts at:    826    1045    2532    2836    3971    4652    4843     826 
   Size:        219    1487     304    1135     681     191    1471

  Fragments arranged by size: 

                1487    1471    1135     681     304     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5488
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5488
 
SmaI CCC'GGG

Cuts at:   1872    2815    1872 
   Size:        943    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2423    3592    4493    4755     820 
   Size:        197     926     480    1169     901     262    1553

  Fragments arranged by size: 

                1553    1169     926     901     480     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3279     493 
   Size:       2786    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5488
 
SphI G_CATG'C

Cuts at:   1132    2846    1132 
   Size:       1714    3774
 
SspI AAT'ATT

Cuts at:      5      52    3412       5 
   Size:         47    3360    2081

  Fragments arranged by size: 

                3360    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5488
 
StyI C'CwwG_G

Cuts at:    513    1065    2927     513 
   Size:        552    1862    3074

  Fragments arranged by size: 

                3074    1862     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3515    3532    3685    3870    4315     325 
   Size:       1255    1935      17     153     185     445    1498

  Fragments arranged by size: 

                1935    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3734    5446      96 
   Size:        274      80      33      51    1443    1757    1712     138

  Fragments arranged by size: 

                1757    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5448    5480    5448 
   Size:         32    5456
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5488
 
XbaI T'CTAG_A

Cuts at:   2824    2824 
   Size:       5488
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5488
 
XmaI C'CCGG_G

Cuts at:   1870    2813    1870 
   Size:        943    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2462    3617    2462 
   Size:       1155    4333
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3296     276 
   Size:         53      83     186     936    1762    2468

  Fragments arranged by size: 

                2468    1762     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI     SacI     SalI     SapI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI    BfrBI
     BplI    BsiWI    BsmBI    BstXI  BstZ17I   DraIII    EcoNI EcoO109I
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    PvuII    RsrII    SacII    SanDI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
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