(Circular) (Six-base) MAPSORT of: pFN21AE2413.seq Check: 4552 from: 1 to: 10636 pFN21AE2413 10636 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2380 5096 7399 2380 Size: 2716 2303 5617 Fragments arranged by size: 5617 2716 2303 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3355 8446 278 Size: 53 83 186 936 1819 5091 2468 Fragments arranged by size: 5091 2468 1819 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5142 5361 7979 1937 Size: 3205 219 2618 4594 Fragments arranged by size: 4594 3205 2618 219 Acc65I G'GTAC_C Cuts at: 4531 7360 7593 7957 4531 Size: 2829 233 364 7210 Fragments arranged by size: 7210 2829 364 233 AclI AA'CG_TT Cuts at: 1563 8763 9136 1563 Size: 7200 373 3063 Fragments arranged by size: 7200 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2037 3312 5841 6050 6629 6839 8699 10083 Size: 405 1275 2529 209 579 210 1860 1384 Cuts at: 10083 10351 1632 Size: 268 1917 Fragments arranged by size: 2529 1917 1860 1384 1275 579 405 268 210 209 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10439 AflIII A'CryG_T Cuts at: 1203 3630 4089 4257 5157 5769 9535 10512 1203 Size: 2427 459 168 900 612 3766 977 1327 Fragments arranged by size: 3766 2427 1327 977 900 612 459 168 AgeI A'CCGG_T Cuts at: 3428 9467 3428 Size: 6039 4597 AhdI GACnn_n'nnGTC Cuts at: 7870 9365 7870 Size: 1495 9141 AleI CACnn'nnGTG Cuts at: 1166 3833 1166 Size: 2667 7969 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2239 3887 5075 7794 1184 Size: 1055 1648 1188 2719 4026 Fragments arranged by size: 4026 2719 1648 1188 1055 AlwNI CAG_nnn'CTG Cuts at: 2069 3525 3918 4126 4307 6652 6697 7426 8379 Size: 1456 393 208 181 2345 45 729 953 Cuts at: 8379 9951 2069 Size: 1572 2754 Fragments arranged by size: 2754 2345 1572 1456 953 729 393 208 181 45 ApaI G_GGCC'C Cuts at: 2100 2555 2922 3758 6371 7089 7497 2100 Size: 455 367 836 2613 718 408 5239 Fragments arranged by size: 5239 2613 836 718 455 408 367 ApaLI G'TGCA_C Cuts at: 2878 8693 9849 2878 Size: 5815 1156 3665 Fragments arranged by size: 5815 3665 1156 ApoI r'AATT_y Cuts at: 1493 5078 6386 6749 6788 7204 7486 7945 8171 Size: 3585 1308 363 39 416 282 459 226 Cuts at: 8171 8323 10432 1493 Size: 152 2109 1697 Fragments arranged by size: 3585 2109 1697 1308 459 416 363 282 226 152 39 AseI AT'TA_AT Cuts at: 160 1032 9190 160 Size: 872 8158 1606 Fragments arranged by size: 8158 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10636 AvaI C'yCGr_G Cuts at: 1870 1943 2093 2211 2665 2771 7740 7961 1870 Size: 73 150 118 454 106 4969 221 4545 Fragments arranged by size: 4969 4545 454 221 150 118 106 73 AvrII C'CTAG_G Cuts at: 2137 2137 Size: 10636 BamHI G'GATC_C Cuts at: 2816 5940 7145 7966 8333 2816 Size: 3124 1205 821 367 5119 Fragments arranged by size: 5119 3124 1205 821 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2707 3845 4372 4531 7353 Size: 325 217 627 920 1138 527 159 2822 Cuts at: 7353 7360 7466 7593 7957 9412 618 Size: 7 106 127 364 1455 1842 Fragments arranged by size: 2822 1842 1455 1138 920 627 527 364 325 217 159 127 106 7 BanII G_rGCy'C Cuts at: 1391 2046 2100 2555 2772 2922 3478 3758 4693 Size: 655 54 455 217 150 556 280 935 Cuts at: 4693 5132 5855 6371 7028 7089 7177 7336 7497 Size: 439 723 516 657 61 88 159 161 Cuts at: 7497 7507 7955 1391 Size: 10 448 4072 Fragments arranged by size: 4072 935 723 657 655 556 516 455 448 439 280 217 161 159 150 88 61 54 10 BbeI G_GCGC'C Cuts at: 3849 3849 Size: 10636 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2850 2965 3207 3613 3765 4612 5680 Size: 971 951 115 242 406 152 847 1068 Cuts at: 5680 928 Size: 5884 Fragments arranged by size: 5884 1068 971 951 847 406 242 152 115 BbvCI CC'TCA_GC Cuts at: 4565 4583 4652 4674 7012 7530 4565 Size: 18 69 22 2338 518 7671 Fragments arranged by size: 7671 2338 518 69 22 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2238 2248 2272 2282 5282 5316 5528 Size: 34 301 10 24 10 3000 34 212 Cuts at: 5528 5562 6498 6532 8827 8861 1903 Size: 34 936 34 2295 34 3678 Fragments arranged by size: 3678 3000 2295 936 301 212 34 34 34 34 34 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 6479 8528 9738 6479 Size: 2049 1210 7377 Fragments arranged by size: 7377 2049 1210 BclI T'GATC_A Cuts at: 1548 6297 6321 1548 Size: 4749 24 5863 Fragments arranged by size: 5863 4749 24 BfrBI ATG'CAT Cuts at: 5297 5297 Size: 10636 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 2647 3306 Size: 107 122 71 631 605 388 587 659 Cuts at: 3306 3670 5234 5501 5844 6857 9247 136 Size: 364 1564 267 343 1013 2390 1525 Fragments arranged by size: 2390 1564 1525 1013 659 631 605 587 388 364 343 267 122 107 71 BglII A'GATC_T Cuts at: 5340 6672 10631 5340 Size: 1332 3959 5345 Fragments arranged by size: 5345 3959 1332 BlpI GC'TnA_GC Cuts at: 8053 8053 Size: 10636 Bme1580I G_kGCm'C Cuts at: 1790 2093 2100 2200 2218 2555 2882 2922 3565 Size: 303 7 100 18 337 327 40 643 Cuts at: 3565 3758 5431 6371 6503 7089 7137 7356 7497 Size: 193 1673 940 132 586 48 219 141 Cuts at: 7497 8697 9853 1790 Size: 1200 1156 2573 Fragments arranged by size: 2573 1673 1200 1156 940 643 586 337 327 303 219 193 141 132 100 48 40 18 7 BmgBI CAC'GTC Cuts at: 1318 2802 5158 1318 Size: 1484 2356 6796 Fragments arranged by size: 6796 2356 1484 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2040 3610 3928 Size: 416 53 83 399 24 623 1570 318 Cuts at: 3928 7083 7122 8367 9325 442 Size: 3155 39 1245 958 1753 Fragments arranged by size: 3155 1753 1570 1245 958 623 416 399 318 83 53 39 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10636 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2377 3277 5881 2377 Size: 900 2604 7132 Fragments arranged by size: 7132 2604 900 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2564 4864 6653 7102 9296 1239 Size: 1166 159 2300 1789 449 2194 2579 Fragments arranged by size: 2579 2300 2194 1789 1166 449 159 Bpu10I CC'TnA_GC Cuts at: 2148 4565 4583 4652 4674 7012 7506 7530 2148 Size: 2417 18 69 22 2338 494 24 5254 Fragments arranged by size: 5254 2417 2338 494 69 24 22 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2420 3216 4946 5100 5483 8761 9626 9924 2420 Size: 796 1730 154 383 3278 865 298 3132 Fragments arranged by size: 3278 3132 1730 865 796 383 298 154 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3437 3818 7006 7737 9299 882 Size: 617 1938 381 3188 731 1562 2219 Fragments arranged by size: 3188 2219 1938 1562 731 617 381 BsaAI yAC'GTr Cuts at: 493 1204 2401 7642 8427 493 Size: 711 1197 5241 785 2702 Fragments arranged by size: 5241 2702 1197 785 711 BsaBI GATnn'nnATC Cuts at: 5393 6662 10630 5393 Size: 1269 3968 5399 Fragments arranged by size: 5399 3968 1269 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2571 3111 3352 3846 Size: 53 83 186 936 1038 540 241 494 Cuts at: 3846 6828 8443 8825 9460 275 Size: 2982 1615 382 635 1451 Fragments arranged by size: 2982 1615 1451 1038 936 635 540 494 382 241 186 83 53 BsaWI w'CCGG_w Cuts at: 3428 4995 6597 9069 9467 9741 9888 10299 3428 Size: 1567 1602 2472 398 274 147 411 3765 Fragments arranged by size: 3765 2472 1602 1567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4787 4817 1178 Size: 30 165 30 3384 30 6997 Fragments arranged by size: 6997 3384 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2849 3479 3482 3881 3980 4097 4412 4445 Size: 1663 630 3 399 99 117 315 33 Cuts at: 4445 4448 4451 4454 4457 6743 6746 6938 6941 Size: 3 3 3 3 2286 3 192 3 Cuts at: 6941 6971 7883 7910 1186 Size: 30 912 27 3912 Fragments arranged by size: 3912 2286 1663 912 630 399 315 192 117 99 33 30 27 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2101 2199 2468 3415 5505 7005 7664 9839 10335 Size: 98 269 947 2090 1500 659 2175 496 Cuts at: 10335 2101 Size: 2402 Fragments arranged by size: 2402 2175 2090 1500 947 659 496 269 98 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2657 2702 4503 4778 4994 5074 5081 Size: 573 921 45 1801 275 216 80 7 Cuts at: 5081 5256 7622 1163 Size: 175 2366 4177 Fragments arranged by size: 4177 2366 1801 921 573 275 216 175 80 45 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8086 8847 9875 1540 Size: 275 186 6085 761 1028 2301 Fragments arranged by size: 6085 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2436 2463 2685 2739 2882 3012 3654 3876 5721 Size: 27 222 54 143 130 642 222 1845 Cuts at: 5721 5855 7310 7336 8697 8782 9853 2436 Size: 134 1455 26 1361 85 1071 3219 Fragments arranged by size: 3219 1845 1455 1361 1071 642 222 222 143 134 130 85 54 27 26 BsmI GAATG_Cn' Cuts at: 2796 5126 8147 8240 2796 Size: 2330 3021 93 5192 Fragments arranged by size: 5192 3021 2330 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2420 5148 6457 7902 2420 Size: 2728 1309 1445 5154 Fragments arranged by size: 5154 2728 1445 1309 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2093 2100 2200 2218 2436 2463 Size: 399 256 47 7 100 18 218 27 Cuts at: 2463 2555 2685 2739 2772 2882 2922 3012 3478 Size: 92 130 54 33 110 40 90 466 Cuts at: 3478 3565 3654 3758 3876 4693 5132 5431 5721 Size: 87 89 104 118 817 439 299 290 Cuts at: 5721 5855 6371 6503 7028 7089 7137 7177 7310 Size: 134 516 132 525 61 48 40 133 Cuts at: 7310 7336 7356 7497 7507 7955 8697 8782 9853 Size: 26 20 141 10 448 742 85 1071 Cuts at: 9853 1391 Size: 2174 Fragments arranged by size: 2174 1071 817 742 525 516 466 448 439 399 299 290 256 218 141 134 133 132 130 118 110 104 100 92 90 89 87 85 61 54 48 47 40 40 33 27 26 20 18 10 7 BspEI T'CCGG_A Cuts at: 4995 6597 10299 4995 Size: 1602 3702 5332 Fragments arranged by size: 5332 3702 1602 BspHI T'CATG_A Cuts at: 2694 3510 5013 5385 8525 10255 2694 Size: 816 1503 372 3140 1730 3075 Fragments arranged by size: 3140 3075 1730 1503 816 372 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2380 3213 5096 7385 7399 7839 7991 844 Size: 1536 833 1883 2289 14 440 152 3489 Fragments arranged by size: 3489 2289 1883 1536 833 440 152 14 BsrBI CCG'CTC Cuts at: 1394 1823 5182 8523 1394 Size: 429 3359 3341 3507 Fragments arranged by size: 3507 3359 3341 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2491 2721 4269 4342 5373 5948 6015 Size: 1183 1243 230 1548 73 1031 575 67 Cuts at: 6015 6540 9131 9305 65 Size: 525 2591 174 1396 Fragments arranged by size: 2591 1548 1396 1243 1183 1031 575 525 230 174 73 67 BsrFI r'CCGG_y Cuts at: 1700 1810 3428 9280 9467 1700 Size: 110 1618 5852 187 2869 Fragments arranged by size: 5852 2869 1618 187 110 BsrGI T'GTAC_A Cuts at: 96 3488 96 Size: 3392 7244 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10636 BssSI C'ACGA_G Cuts at: 2732 8696 9708 2732 Size: 5964 1012 3660 Fragments arranged by size: 5964 3660 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2680 4307 4972 794 Size: 1886 1627 665 6458 Fragments arranged by size: 6458 1886 1627 665 BstBI TT'CG_AA Cuts at: 9440 9440 Size: 10636 BstEII G'GTnAC_C Cuts at: 1184 2205 3907 6736 1184 Size: 1021 1702 2829 5084 Fragments arranged by size: 5084 2829 1702 1021 BstXI CCAn_nnnn'nTGG Cuts at: 2323 3835 4276 4894 2323 Size: 1512 441 618 8065 Fragments arranged by size: 8065 1512 618 441 BstYI r'GATC_y Cuts at: 1972 2816 3681 3897 4122 4345 4834 5340 5940 Size: 844 865 216 225 223 489 506 600 Cuts at: 5940 6396 6672 7145 7604 7966 8333 8718 8735 Size: 456 276 473 459 362 367 385 17 Cuts at: 8735 10187 10273 10285 10631 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 865 844 600 506 489 473 459 456 385 367 362 346 276 225 223 216 86 17 12 BstZ17I GTA'TAC Cuts at: 5143 5143 Size: 10636 Bsu36I CC'TnA_GG Cuts at: 2352 3893 4542 2352 Size: 1541 649 8446 Fragments arranged by size: 8446 1541 649 BtgI C'CryG_G Cuts at: 513 1065 2902 2988 3030 3655 4798 5311 5667 Size: 552 1837 86 42 625 1143 513 356 Cuts at: 5667 6741 7669 513 Size: 1074 928 3480 Fragments arranged by size: 3480 1837 1143 1074 928 625 552 513 356 86 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2829 3468 3625 3955 5713 5989 Size: 683 56 1603 639 157 330 1758 276 Cuts at: 5989 6241 487 Size: 252 4882 Fragments arranged by size: 4882 1758 1603 683 639 330 276 252 157 56 BtsI GCAGTG_nn' Cuts at: 2128 2193 2367 2726 3585 4305 4663 4982 5273 Size: 65 174 359 859 720 358 319 291 Cuts at: 5273 7616 7690 8152 8946 8966 2128 Size: 2343 74 462 794 20 3798 Fragments arranged by size: 3798 2343 859 794 720 462 359 358 319 291 174 74 65 20 ClaI AT'CG_AT Cuts at: 1554 5292 1554 Size: 3738 6898 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5815 5850 6568 6603 529 Size: 35 5251 35 718 35 4562 Fragments arranged by size: 5251 4562 718 35 35 35 DraI TTT'AAA Cuts at: 7940 7940 Size: 10636 DraIII CAC_nnn'GTG Cuts at: 2680 6511 2680 Size: 3831 6805 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9643 809 Size: 507 8327 1802 Fragments arranged by size: 8327 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2235 2561 Size: 54 1425 50 275 18 360 45 326 Cuts at: 2561 2639 2912 3314 3324 3480 4287 4668 4989 Size: 78 273 402 10 156 807 381 321 Cuts at: 4989 5836 7764 8083 8972 10601 8 Size: 847 1928 319 889 1629 43 Fragments arranged by size: 1928 1629 1425 889 847 807 402 381 360 326 321 319 275 273 156 78 54 50 45 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 8083 1537 Size: 275 6271 4090 Fragments arranged by size: 6271 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2225 2744 3234 3288 3998 4004 4232 4325 Size: 872 519 490 54 710 6 228 93 Cuts at: 4325 4389 4446 4455 4464 4470 5072 5705 5900 Size: 64 57 9 9 6 602 633 195 Cuts at: 5900 6003 6069 6222 6850 6930 7566 7670 8566 Size: 103 66 153 628 80 636 104 896 Cuts at: 8566 1353 Size: 3423 Fragments arranged by size: 3423 896 872 710 636 633 628 602 519 490 228 195 153 104 103 93 80 66 64 57 54 9 9 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3253 3527 5504 6811 6838 9223 Size: 1064 138 1664 274 1977 1307 27 2385 Cuts at: 9223 9597 9743 387 Size: 374 146 1280 Fragments arranged by size: 2385 1977 1664 1307 1280 1064 374 274 146 138 27 EcoICRI GAG'CTC Cuts at: 5853 7334 5853 Size: 1481 9155 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2405 2564 3312 4864 5841 6050 Size: 393 405 368 159 748 1552 977 209 Cuts at: 6050 6629 6653 6839 7102 8699 9296 10083 10351 Size: 579 24 186 263 1597 597 787 268 Cuts at: 10351 1239 Size: 1524 Fragments arranged by size: 1597 1552 1524 977 787 748 597 579 405 393 368 268 263 209 186 159 24 EcoNI CCTnn'n_nnAGG Cuts at: 2387 2387 Size: 10636 EcoO109I rG'GnC_Cy Cuts at: 2066 2097 2142 2551 2552 2662 2839 2918 3081 Size: 31 45 409 1 110 177 79 163 Cuts at: 3081 3256 3553 3755 4102 4678 4785 6368 6887 Size: 175 297 202 347 576 107 1583 519 Cuts at: 6887 6904 7493 2066 Size: 17 589 5209 Fragments arranged by size: 5209 1583 589 576 519 409 347 297 202 177 175 163 110 107 79 45 31 17 1 EcoRI G'AATT_C Cuts at: 6386 7945 8323 6386 Size: 1559 378 8699 Fragments arranged by size: 8699 1559 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4069 5149 5767 7426 4069 Size: 1080 618 1659 7279 Fragments arranged by size: 7279 1659 1080 618 FspI TGC'GCA Cuts at: 7622 9142 7622 Size: 1520 9116 HaeII r_GCGC'y Cuts at: 1678 2475 2911 3849 4554 4887 7435 9783 1678 Size: 797 436 938 705 333 2548 2348 2531 Fragments arranged by size: 2548 2531 2348 938 797 705 436 333 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2325 2357 2828 2860 6191 Size: 32 135 32 753 32 471 32 3331 Cuts at: 6191 6223 6289 6321 7061 7093 7856 7888 9277 Size: 32 66 32 740 32 763 32 1389 Cuts at: 9277 9309 9351 9383 1373 Size: 32 42 32 2626 Fragments arranged by size: 3331 2626 1389 763 753 740 471 135 66 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3636 5194 7980 8226 669 Size: 988 281 1698 1558 2786 246 3079 Fragments arranged by size: 3079 2786 1698 1558 988 281 246 HindIII A'AGCT_T Cuts at: 748 5918 748 Size: 5170 5466 HpaI GTT'AAC Cuts at: 8226 8226 Size: 10636 KasI G'GCGC_C Cuts at: 3845 3845 Size: 10636 KpnI G_GTAC'C Cuts at: 4535 7364 7597 7961 4535 Size: 2829 233 364 7210 Fragments arranged by size: 7210 2829 364 233 MfeI C'AATT_G Cuts at: 8235 8235 Size: 10636 MluI A'CGCG_T Cuts at: 10512 10512 Size: 10636 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 2388 2844 4812 7173 7379 9750 9934 Size: 684 399 456 1968 2361 206 2371 184 Cuts at: 9934 1305 Size: 2007 Fragments arranged by size: 2371 2361 2007 1968 684 456 399 206 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2192 2237 2914 3482 4289 Size: 54 1425 343 360 45 677 568 807 Cuts at: 4289 4991 5838 7766 10 Size: 702 847 1928 2880 Fragments arranged by size: 2880 1928 1425 847 807 702 677 568 360 343 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2516 3833 3869 4274 4614 4892 5057 Size: 648 1350 1317 36 405 340 278 165 Cuts at: 5057 5399 6008 6038 6044 6381 6444 8594 8953 Size: 342 609 30 6 337 63 2150 359 Cuts at: 8953 9112 518 Size: 159 2042 Fragments arranged by size: 2150 2042 1350 1317 648 609 405 359 342 340 337 278 165 159 63 36 30 6 MspA1I CmG'CkG Cuts at: 1606 2105 2203 2713 2904 3178 3322 3486 4033 Size: 499 98 510 191 274 144 164 547 Cuts at: 4033 4582 4673 4800 7534 7558 7583 8052 8729 Size: 549 91 127 2734 24 25 469 677 Cuts at: 8729 9877 10122 1606 Size: 1148 245 2120 Fragments arranged by size: 2734 2120 1148 677 549 547 510 499 469 274 245 191 164 144 127 98 91 25 24 NarI GG'CG_CC Cuts at: 3846 3846 Size: 10636 NcoI C'CATG_G Cuts at: 513 1065 2988 3030 5667 7669 513 Size: 552 1923 42 2637 2002 3480 Fragments arranged by size: 3480 2637 2002 1923 552 42 NdeI CA'TA_TG Cuts at: 387 4174 5299 387 Size: 3787 1125 5724 Fragments arranged by size: 5724 3787 1125 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10636 NotI GC'GGCC_GC Cuts at: 8083 8083 Size: 10636 NsiI A_TGCA'T Cuts at: 5299 5299 Size: 10636 NspI r_CATG'y Cuts at: 1132 1229 4030 4970 6043 6544 7994 9539 1132 Size: 97 2801 940 1073 501 1450 1545 2229 Fragments arranged by size: 2801 2229 1545 1450 1073 940 501 97 PasI CC'CwG_GG Cuts at: 1348 2329 3048 5431 6416 1348 Size: 981 719 2383 985 5568 Fragments arranged by size: 5568 2383 985 981 719 PciI A'CATG_T Cuts at: 9535 9535 Size: 10636 PflMI CCAn_nnn'nTGG Cuts at: 1108 2057 2383 3918 4714 6354 1108 Size: 949 326 1535 796 1640 5390 Fragments arranged by size: 5390 1640 1535 949 796 326 PfoI T'CCnGG_A Cuts at: 3684 5175 6184 6771 7028 3684 Size: 1491 1009 587 257 7292 Fragments arranged by size: 7292 1491 1009 587 257 PmeI GTTT'AAAC Cuts at: 7940 7940 Size: 10636 PmlI CAC'GTG Cuts at: 7642 7642 Size: 10636 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7837 7869 8705 8737 10218 10250 7837 Size: 32 836 32 1481 32 8223 Fragments arranged by size: 8223 1481 836 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2142 2662 2839 3081 3256 3553 4678 6887 2142 Size: 520 177 242 175 297 1125 2209 5891 Fragments arranged by size: 5891 2209 1125 520 297 242 177 175 PshAI GACnn'nnGTC Cuts at: 1613 6885 1613 Size: 5272 5364 PsiI TTA'TAA Cuts at: 8206 8206 Size: 10636 PspOMI G'GGCC_C Cuts at: 2096 2551 2918 3754 6367 7085 7493 2096 Size: 455 367 836 2613 718 408 5239 Fragments arranged by size: 5239 2613 836 718 455 408 367 PspXI vC'TCGA_Gb Cuts at: 2771 2771 Size: 10636 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5576 5608 5576 Size: 32 10604 PstI C_TGCA'G Cuts at: 830 4506 6879 7396 7431 7988 830 Size: 3676 2373 517 35 557 3478 Fragments arranged by size: 3676 3478 2373 557 517 35 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10636 PvuII CAG'CTG Cuts at: 2105 3322 3486 4033 2105 Size: 1217 164 547 8708 Fragments arranged by size: 8708 1217 547 164 SacI G_AGCT'C Cuts at: 5855 7336 5855 Size: 1481 9155 SacII CC_GC'GG Cuts at: 2905 4801 2905 Size: 1896 8740 SalI G'TCGA_C Cuts at: 1936 7978 1936 Size: 6042 4594 SanDI GG'GwC_CC Cuts at: 2142 2662 2142 Size: 520 10116 SapI GCTCTTCn'nnn_ Cuts at: 2744 3234 4325 5072 6003 6069 2744 Size: 490 1091 747 931 66 7311 Fragments arranged by size: 7311 1091 931 747 490 66 SbfI CC_TGCA'GG Cuts at: 4506 6879 7396 7988 4506 Size: 2373 517 592 7154 Fragments arranged by size: 7154 2373 592 517 ScaI AGT'ACT Cuts at: 4914 4923 8884 10596 4914 Size: 9 3961 1712 4954 Fragments arranged by size: 4954 3961 1712 9 SexAI A'CCwGG_T Cuts at: 7356 7356 Size: 10636 SfcI C'TryA_G Cuts at: 826 1045 4502 6875 7392 7427 7858 7984 9119 Size: 219 3457 2373 517 35 431 126 1135 Cuts at: 9119 9800 9991 826 Size: 681 191 1471 Fragments arranged by size: 3457 2373 1471 1135 681 517 431 219 191 126 35 SfiI GGCCn_nnn'nGGCC Cuts at: 3306 3306 Size: 10636 SfoI GGC'GCC Cuts at: 3847 3847 Size: 10636 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10636 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10636 SmaI CCC'GGG Cuts at: 1872 2095 7963 1872 Size: 223 5868 4545 Fragments arranged by size: 5868 4545 223 SmlI C'TyrA_G Cuts at: 820 1017 1943 2435 2771 3231 4961 5115 5498 Size: 197 926 492 336 460 1730 154 383 Cuts at: 5498 7740 8740 9641 9903 820 Size: 2242 1000 901 262 1553 Fragments arranged by size: 2242 1730 1553 1000 926 901 492 460 383 336 262 197 154 SnaBI TAC'GTA Cuts at: 493 2401 8427 493 Size: 1908 6026 2702 Fragments arranged by size: 6026 2702 1908 SpeI A'CTAG_T Cuts at: 152 152 Size: 10636 SphI G_CATG'C Cuts at: 1132 4030 6043 7994 1132 Size: 2898 2013 1951 3774 Fragments arranged by size: 3774 2898 2013 1951 SspI AAT'ATT Cuts at: 5 52 8560 5 Size: 47 8508 2081 Fragments arranged by size: 8508 2081 47 StuI AGG'CCT Cuts at: 1517 2136 4368 5981 1517 Size: 619 2232 1613 6172 Fragments arranged by size: 6172 2232 1613 619 StyI C'CwwG_G Cuts at: 513 1065 2137 2782 2988 3030 3132 3277 3448 Size: 552 1072 645 206 42 102 145 171 Cuts at: 3448 3831 3858 4311 4518 4953 5667 5842 6423 Size: 383 27 453 207 435 714 175 581 Cuts at: 6423 7215 7669 8075 513 Size: 792 454 406 3074 Fragments arranged by size: 3074 1072 792 714 645 581 552 454 453 435 406 383 207 206 175 171 145 102 42 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3017 3040 3692 7169 8663 8680 8833 Size: 1255 1437 23 652 3477 1494 17 153 Cuts at: 8833 9018 9463 325 Size: 185 445 1498 Fragments arranged by size: 3477 1498 1494 1437 1255 652 445 185 153 23 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3054 3488 4912 Size: 274 80 33 51 1443 1077 434 1424 Cuts at: 4912 4921 8882 10594 96 Size: 9 3961 1712 138 Fragments arranged by size: 3961 1712 1443 1424 1077 434 274 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4188 4220 4787 4819 10596 10628 4188 Size: 32 567 32 5777 32 4196 Fragments arranged by size: 5777 4196 567 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5216 1316 Size: 3900 6736 XbaI T'CTAG_A Cuts at: 7972 7972 Size: 10636 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2380 3856 4525 4633 4750 7831 2380 Size: 1476 669 108 117 3081 5185 Fragments arranged by size: 5185 3081 1476 669 117 108 XhoI C'TCGA_G Cuts at: 1943 2771 7740 1943 Size: 828 4969 4839 Fragments arranged by size: 4969 4839 828 XmaI C'CCGG_G Cuts at: 1870 2093 7961 1870 Size: 223 5868 4545 Fragments arranged by size: 5868 4545 223 XmnI GAAnn'nnTTC Cuts at: 2282 5396 8765 2282 Size: 3114 3369 4153 Fragments arranged by size: 4153 3369 3114 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3353 8444 276 Size: 53 83 186 936 1819 5091 2468 Fragments arranged by size: 5091 2468 1819 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BsiWI EcoRV FseI FspAI NaeI NgoMIV NruI PacI RsrII SrfI SwaI