Restriction map for
pFN21AE2413
(Linear) (Six-base) MAPSORT of: KIEE2413.seq Check: 952 from: 1 to: 5931
KIEE2413.orf
With 182 enzymes: SgfI *
July 23, 2012 18:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 375 3091 5394 5931
Size: 375 2716 2303 537
Fragments arranged by size:
2716 2303 537 375
AatII G_ACGT'C
Cuts at: 0 1350 5931
Size: 1350 4581
AccI GT'mk_AC
Cuts at: 0 3137 3356 5931
Size: 3137 219 2575
Fragments arranged by size:
3137 2575 219
Acc65I G'GTAC_C
Cuts at: 0 2526 5355 5588 5931
Size: 2526 2829 233 343
Fragments arranged by size:
2829 2526 343 233
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 1307 3836 4045 4624 4834 5931
Size: 32 1275 2529 209 579 210 1097
Fragments arranged by size:
2529 1275 1097 579 210 209 32
AflIII A'CryG_T
Cuts at: 0 1625 2084 2252 3152 3764 5931
Size: 1625 459 168 900 612 2167
Fragments arranged by size:
2167 1625 900 612 459 168
AgeI A'CCGG_T
Cuts at: 0 1423 5931
Size: 1423 4508
AhdI GACnn_n'nnGTC
Cuts at: 0 5865 5931
Size: 5865 66
AleI CACnn'nnGTG
Cuts at: 0 1828 5931
Size: 1828 4103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 234 1882 3070 5789 5931
Size: 234 1648 1188 2719 142
Fragments arranged by size:
2719 1648 1188 234 142
AlwNI CAG_nnn'CTG
Cuts at: 0 64 1520 1913 2121 2302 4647 4692 5421
Size: 64 1456 393 208 181 2345 45 729
Cuts at: 5421 5931
Size: 510
Fragments arranged by size:
2345 1456 729 510 393 208 181 64
45
ApaI G_GGCC'C
Cuts at: 0 95 550 917 1753 4366 5084 5492 5931
Size: 95 455 367 836 2613 718 408 439
Fragments arranged by size:
2613 836 718 455 439 408 367 95
ApaLI G'TGCA_C
Cuts at: 0 873 5931
Size: 873 5058
ApoI r'AATT_y
Cuts at: 0 3073 4381 4744 4783 5199 5481 5931
Size: 3073 1308 363 39 416 282 450
Fragments arranged by size:
3073 1308 450 416 363 282 39
AvaI C'yCGr_G
Cuts at: 0 88 206 660 766 5735 5931
Size: 88 118 454 106 4969 196
Fragments arranged by size:
4969 454 196 118 106 88
AvrII C'CTAG_G
Cuts at: 0 132 5931
Size: 132 5799
BamHI G'GATC_C
Cuts at: 0 811 3935 5140 5931
Size: 811 3124 1205 791
Fragments arranged by size:
3124 1205 811 791
BanI G'GyrC_C
Cuts at: 0 702 1840 2367 2526 5348 5355 5461 5588
Size: 702 1138 527 159 2822 7 106 127
Cuts at: 5588 5931
Size: 343
Fragments arranged by size:
2822 1138 702 527 343 159 127 106
7
BanII G_rGCy'C
Cuts at: 0 41 95 550 767 917 1473 1753 2688
Size: 41 54 455 217 150 556 280 935
Cuts at: 2688 3127 3850 4366 5023 5084 5172 5331 5492
Size: 439 723 516 657 61 88 159 161
Cuts at: 5492 5502 5931
Size: 10 429
Fragments arranged by size:
935 723 657 556 516 455 439 429
280 217 161 159 150 88 61 54
41 10
BbeI G_GCGC'C
Cuts at: 0 1844 5931
Size: 1844 4087
BbsI GAAGACnn'nnnn_
Cuts at: 0 845 960 1202 1608 1760 2607 3675 5931
Size: 845 115 242 406 152 847 1068 2256
Fragments arranged by size:
2256 1068 847 845 406 242 152 115
BbvCI CC'TCA_GC
Cuts at: 0 2560 2578 2647 2669 5007 5525 5931
Size: 2560 18 69 22 2338 518 406
Fragments arranged by size:
2560 2338 518 406 69 22 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 233 243 267 277 3277 3311 3523 3557
Size: 233 10 24 10 3000 34 212 34
Cuts at: 3557 4493 4527 5931
Size: 936 34 1404
Fragments arranged by size:
3000 1404 936 233 212 34 34 34
24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 4474 5931
Size: 4474 1457
BclI T'GATC_A
Cuts at: 0 4292 4316 5931
Size: 4292 24 1615
Fragments arranged by size:
4292 1615 24
BfrBI ATG'CAT
Cuts at: 0 3292 5931
Size: 3292 2639
BglI GCCn_nnn'nGGC
Cuts at: 0 55 642 1301 1665 3229 3496 3839 4852
Size: 55 587 659 364 1564 267 343 1013
Cuts at: 4852 5931
Size: 1079
Fragments arranged by size:
1564 1079 1013 659 587 364 343 267
55
BglII A'GATC_T
Cuts at: 0 3335 4667 5931
Size: 3335 1332 1264
Fragments arranged by size:
3335 1332 1264
Bme1580I G_kGCm'C
Cuts at: 0 88 95 195 213 550 877 917 1560
Size: 88 7 100 18 337 327 40 643
Cuts at: 1560 1753 3426 4366 4498 5084 5132 5351 5492
Size: 193 1673 940 132 586 48 219 141
Cuts at: 5492 5931
Size: 439
Fragments arranged by size:
1673 940 643 586 439 337 327 219
193 141 132 100 88 48 40 18
7
BmgBI CAC'GTC
Cuts at: 0 797 3153 5931
Size: 797 2356 2778
Fragments arranged by size:
2778 2356 797
BmrI ACTGGGnnnn_n'
Cuts at: 0 35 1605 1923 5078 5117 5931
Size: 35 1570 318 3155 39 814
Fragments arranged by size:
3155 1570 814 318 39 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 372 1272 3876 5931
Size: 372 900 2604 2055
Fragments arranged by size:
2604 2055 900 372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 559 2859 4648 5097 5931
Size: 400 159 2300 1789 449 834
Fragments arranged by size:
2300 1789 834 449 400 159
Bpu10I CC'TnA_GC
Cuts at: 0 143 2560 2578 2647 2669 5007 5501 5525
Size: 143 2417 18 69 22 2338 494 24
Cuts at: 5525 5931
Size: 406
Fragments arranged by size:
2417 2338 494 406 143 69 24 22
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1211 2941 3095 3478 5931
Size: 415 796 1730 154 383 2453
Fragments arranged by size:
2453 1730 796 415 383 154
BsaI GGTCTCn'nnnn_
Cuts at: 0 1432 1813 5001 5732 5931
Size: 1432 381 3188 731 199
Fragments arranged by size:
3188 1432 731 381 199
BsaAI yAC'GTr
Cuts at: 0 396 5637 5931
Size: 396 5241 294
Fragments arranged by size:
5241 396 294
BsaBI GATnn'nnATC
Cuts at: 0 3388 4657 5931
Size: 3388 1269 1274
Fragments arranged by size:
3388 1274 1269
BsaHI Gr'CG_yC
Cuts at: 0 566 1106 1347 1841 4823 5931
Size: 566 540 241 494 2982 1108
Fragments arranged by size:
2982 1108 566 540 494 241
BsaWI w'CCGG_w
Cuts at: 0 1423 2990 4592 5931
Size: 1423 1567 1602 1339
Fragments arranged by size:
1602 1567 1423 1339
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2782 2812 5931
Size: 2782 30 3119
Fragments arranged by size:
3119 2782 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 844 1474 1477 1876 1975 2092 2407 2440
Size: 844 630 3 399 99 117 315 33
Cuts at: 2440 2443 2446 2449 2452 4738 4741 4933 4936
Size: 3 3 3 3 2286 3 192 3
Cuts at: 4936 4966 5878 5905 5931
Size: 30 912 27 26
Fragments arranged by size:
2286 912 844 630 399 315 192 117
99 33 30 27 26 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 96 194 463 1410 3500 5000 5659 5931
Size: 96 98 269 947 2090 1500 659 272
Fragments arranged by size:
2090 1500 947 659 272 269 98 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 697 2498 2773 2989 3069 3076 3251
Size: 652 45 1801 275 216 80 7 175
Cuts at: 3251 5617 5931
Size: 2366 314
Fragments arranged by size:
2366 1801 652 314 275 216 175 80
45 7
BsiHKAI G_wGCw'C
Cuts at: 0 431 458 680 734 877 1007 1649 1871
Size: 431 27 222 54 143 130 642 222
Cuts at: 1871 3716 3850 5305 5331 5931
Size: 1845 134 1455 26 600
Fragments arranged by size:
1845 1455 642 600 431 222 222 143
134 130 54 27 26
BsmI GAATG_Cn'
Cuts at: 0 791 3121 5931
Size: 791 2330 2810
Fragments arranged by size:
2810 2330 791
BsmBI CGTCTCn'nnnn_
Cuts at: 0 415 3143 4452 5897 5931
Size: 415 2728 1309 1445 34
Fragments arranged by size:
2728 1445 1309 415 34
Bsp1286I G_dGCh'C
Cuts at: 0 41 88 95 195 213 431 458 550
Size: 41 47 7 100 18 218 27 92
Cuts at: 550 680 734 767 877 917 1007 1473 1560
Size: 130 54 33 110 40 90 466 87
Cuts at: 1560 1649 1753 1871 2688 3127 3426 3716 3850
Size: 89 104 118 817 439 299 290 134
Cuts at: 3850 4366 4498 5023 5084 5132 5172 5305 5331
Size: 516 132 525 61 48 40 133 26
Cuts at: 5331 5351 5492 5502 5931
Size: 20 141 10 429
Fragments arranged by size:
817 525 516 466 439 429 299 290
218 141 134 133 132 130 118 110
104 100 92 90 89 87 61 54
48 47 41 40 40 33 27 26
20 18 10 7
BspEI T'CCGG_A
Cuts at: 0 2990 4592 5931
Size: 2990 1602 1339
Fragments arranged by size:
2990 1602 1339
BspHI T'CATG_A
Cuts at: 0 689 1505 3008 3380 5931
Size: 689 816 1503 372 2551
Fragments arranged by size:
2551 1503 816 689 372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 375 1208 3091 5380 5394 5834 5931
Size: 375 833 1883 2289 14 440 97
Fragments arranged by size:
2289 1883 833 440 375 97 14
BsrBI CCG'CTC
Cuts at: 0 3177 5931
Size: 3177 2754
BsrDI GCAATG_nn'
Cuts at: 0 486 716 2264 2337 3368 3943 4010 4535
Size: 486 230 1548 73 1031 575 67 525
Cuts at: 4535 5931
Size: 1396
Fragments arranged by size:
1548 1396 1031 575 525 486 230 73
67
BsrFI r'CCGG_y
Cuts at: 0 1423 5931
Size: 1423 4508
BsrGI T'GTAC_A
Cuts at: 0 1483 5931
Size: 1483 4448
BssSI C'ACGA_G
Cuts at: 0 727 5931
Size: 727 5204
BstAPI GCAn_nnn'nTGC
Cuts at: 0 675 2302 2967 5931
Size: 675 1627 665 2964
Fragments arranged by size:
2964 1627 675 665
BstEII G'GTnAC_C
Cuts at: 0 200 1902 4731 5931
Size: 200 1702 2829 1200
Fragments arranged by size:
2829 1702 1200 200
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 1830 2271 2889 5931
Size: 318 1512 441 618 3042
Fragments arranged by size:
3042 1512 618 441 318
BstYI r'GATC_y
Cuts at: 0 811 1676 1892 2117 2340 2829 3335 3935
Size: 811 865 216 225 223 489 506 600
Cuts at: 3935 4391 4667 5140 5599 5931
Size: 456 276 473 459 332
Fragments arranged by size:
865 811 600 506 489 473 459 456
332 276 225 223 216
BstZ17I GTA'TAC
Cuts at: 0 3138 5931
Size: 3138 2793
Bsu36I CC'TnA_GG
Cuts at: 0 347 1888 2537 5931
Size: 347 1541 649 3394
Fragments arranged by size:
3394 1541 649 347
BtgI C'CryG_G
Cuts at: 0 897 983 1025 1650 2793 3306 3662 4736
Size: 897 86 42 625 1143 513 356 1074
Cuts at: 4736 5664 5931
Size: 928 267
Fragments arranged by size:
1143 1074 928 897 625 513 356 267
86 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 824 1463 1620 1950 3708 3984 4236 5931
Size: 824 639 157 330 1758 276 252 1695
Fragments arranged by size:
1758 1695 824 639 330 276 252 157
BtsI GCAGTG_nn'
Cuts at: 0 123 188 362 721 1580 2300 2658 2977
Size: 123 65 174 359 859 720 358 319
Cuts at: 2977 3268 5611 5685 5931
Size: 291 2343 74 246
Fragments arranged by size:
2343 859 720 359 358 319 291 246
174 123 74 65
ClaI AT'CG_AT
Cuts at: 0 3287 5931
Size: 3287 2644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3810 3845 4563 4598 5931
Size: 3810 35 718 35 1333
Fragments arranged by size:
3810 1333 718 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 675 4506 5931
Size: 675 3831 1425
Fragments arranged by size:
3831 1425 675
EaeI y'GGCC_r
Cuts at: 0 185 230 556 634 907 1309 1319 1475
Size: 185 45 326 78 273 402 10 156
Cuts at: 1475 2282 2663 2984 3831 5759 5931
Size: 807 381 321 847 1928 172
Fragments arranged by size:
1928 847 807 402 381 326 321 273
185 172 156 78 45 10
EarI CTCTTCn'nnn_
Cuts at: 0 220 739 1229 1283 1993 1999 2227 2320
Size: 220 519 490 54 710 6 228 93
Cuts at: 2320 2384 2441 2450 2459 2465 3067 3700 3895
Size: 64 57 9 9 6 602 633 195
Cuts at: 3895 3998 4064 4217 4845 4925 5561 5665 5931
Size: 103 66 153 628 80 636 104 266
Fragments arranged by size:
710 636 633 628 602 519 490 266
228 220 195 153 104 103 93 80
66 64 57 54 9 9 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1248 1522 3499 4806 4833 5931
Size: 1248 274 1977 1307 27 1098
Fragments arranged by size:
1977 1307 1248 1098 274 27
EcoICRI GAG'CTC
Cuts at: 0 3848 5329 5931
Size: 3848 1481 602
Fragments arranged by size:
3848 1481 602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 400 559 1307 2859 3836 4045 4624
Size: 32 368 159 748 1552 977 209 579
Cuts at: 4624 4648 4834 5097 5931
Size: 24 186 263 834
Fragments arranged by size:
1552 977 834 748 579 368 263 209
186 159 32 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 382 5931
Size: 382 5549
EcoO109I rG'GnC_Cy
Cuts at: 0 61 92 137 546 547 657 834 913
Size: 61 31 45 409 1 110 177 79
Cuts at: 913 1076 1251 1548 1750 2097 2673 2780 4363
Size: 163 175 297 202 347 576 107 1583
Cuts at: 4363 4882 4899 5488 5931
Size: 519 17 589 443
Fragments arranged by size:
1583 589 576 519 443 409 347 297
202 177 175 163 110 107 79 61
45 31 17 1
EcoRI G'AATT_C
Cuts at: 0 4381 5931
Size: 4381 1550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2064 3144 3762 5421 5931
Size: 2064 1080 618 1659 510
Fragments arranged by size:
2064 1659 1080 618 510
FspI TGC'GCA
Cuts at: 0 5617 5931
Size: 5617 314
HaeII r_GCGC'y
Cuts at: 0 470 906 1844 2549 2882 5430 5931
Size: 470 436 938 705 333 2548 501
Fragments arranged by size:
2548 938 705 501 470 436 333
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 320 352 823 855 4186 4218 4284 4316
Size: 320 32 471 32 3331 32 66 32
Cuts at: 4316 5056 5088 5851 5883 5931
Size: 740 32 763 32 48
Fragments arranged by size:
3331 763 740 471 320 66 48 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1631 3189 5931
Size: 1631 1558 2742
Fragments arranged by size:
2742 1631 1558
HindIII A'AGCT_T
Cuts at: 0 3913 5931
Size: 3913 2018
KasI G'GCGC_C
Cuts at: 0 1840 5931
Size: 1840 4091
KpnI G_GTAC'C
Cuts at: 0 2530 5359 5592 5931
Size: 2530 2829 233 339
Fragments arranged by size:
2829 2530 339 233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 839 2807 5168 5374 5931
Size: 383 456 1968 2361 206 557
Fragments arranged by size:
2361 1968 557 456 383 206
MscI TGG'CCA
Cuts at: 0 187 232 909 1477 2284 2986 3833 5761
Size: 187 45 677 568 807 702 847 1928
Cuts at: 5761 5931
Size: 170
Fragments arranged by size:
1928 847 807 702 677 568 187 170
45
MslI CAynn'nnrTG
Cuts at: 0 511 1828 1864 2269 2609 2887 3052 3394
Size: 511 1317 36 405 340 278 165 342
Cuts at: 3394 4003 4033 4039 4376 4439 5931
Size: 609 30 6 337 63 1492
Fragments arranged by size:
1492 1317 609 511 405 342 340 337
278 165 63 36 30 6
MspA1I CmG'CkG
Cuts at: 0 100 198 708 899 1173 1317 1481 2028
Size: 100 98 510 191 274 144 164 547
Cuts at: 2028 2577 2668 2795 5529 5553 5578 5931
Size: 549 91 127 2734 24 25 353
Fragments arranged by size:
2734 549 547 510 353 274 191 164
144 127 100 98 91 25 24
NarI GG'CG_CC
Cuts at: 0 1841 5931
Size: 1841 4090
NcoI C'CATG_G
Cuts at: 0 983 1025 3662 5664 5931
Size: 983 42 2637 2002 267
Fragments arranged by size:
2637 2002 983 267 42
NdeI CA'TA_TG
Cuts at: 0 2169 3294 5931
Size: 2169 1125 2637
Fragments arranged by size:
2637 2169 1125
NsiI A_TGCA'T
Cuts at: 0 3294 5931
Size: 3294 2637
NspI r_CATG'y
Cuts at: 0 2025 2965 4038 4539 5931
Size: 2025 940 1073 501 1392
Fragments arranged by size:
2025 1392 1073 940 501
PasI CC'CwG_GG
Cuts at: 0 324 1043 3426 4411 5931
Size: 324 719 2383 985 1520
Fragments arranged by size:
2383 1520 985 719 324
PflMI CCAn_nnn'nTGG
Cuts at: 0 52 378 1913 2709 4349 5931
Size: 52 326 1535 796 1640 1582
Fragments arranged by size:
1640 1582 1535 796 326 52
PfoI T'CCnGG_A
Cuts at: 0 1679 3170 4179 4766 5023 5931
Size: 1679 1491 1009 587 257 908
Fragments arranged by size:
1679 1491 1009 908 587 257
PmlI CAC'GTG
Cuts at: 0 5637 5931
Size: 5637 294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5832 5864 5931
Size: 5832 32 67
Fragments arranged by size:
5832 67 32
PpuMI rG'GwC_Cy
Cuts at: 0 137 657 834 1076 1251 1548 2673 4882
Size: 137 520 177 242 175 297 1125 2209
Cuts at: 4882 5931
Size: 1049
Fragments arranged by size:
2209 1125 1049 520 297 242 177 175
137
PshAI GACnn'nnGTC
Cuts at: 0 4880 5931
Size: 4880 1051
PspOMI G'GGCC_C
Cuts at: 0 91 546 913 1749 4362 5080 5488 5931
Size: 91 455 367 836 2613 718 408 443
Fragments arranged by size:
2613 836 718 455 443 408 367 91
PspXI vC'TCGA_Gb
Cuts at: 0 766 5931
Size: 766 5165
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3571 3603 5931
Size: 3571 32 2328
Fragments arranged by size:
3571 2328 32
PstI C_TGCA'G
Cuts at: 0 2501 4874 5391 5426 5931
Size: 2501 2373 517 35 505
Fragments arranged by size:
2501 2373 517 505 35
PvuII CAG'CTG
Cuts at: 0 100 1317 1481 2028 5931
Size: 100 1217 164 547 3903
Fragments arranged by size:
3903 1217 547 164 100
SacI G_AGCT'C
Cuts at: 0 3850 5331 5931
Size: 3850 1481 600
Fragments arranged by size:
3850 1481 600
SacII CC_GC'GG
Cuts at: 0 900 2796 5931
Size: 900 1896 3135
Fragments arranged by size:
3135 1896 900
SanDI GG'GwC_CC
Cuts at: 0 137 657 5931
Size: 137 520 5274
Fragments arranged by size:
5274 520 137
SapI GCTCTTCn'nnn_
Cuts at: 0 739 1229 2320 3067 3998 4064 5931
Size: 739 490 1091 747 931 66 1867
Fragments arranged by size:
1867 1091 931 747 739 490 66
SbfI CC_TGCA'GG
Cuts at: 0 2501 4874 5391 5931
Size: 2501 2373 517 540
Fragments arranged by size:
2501 2373 540 517
ScaI AGT'ACT
Cuts at: 0 2909 2918 5931
Size: 2909 9 3013
Fragments arranged by size:
3013 2909 9
SexAI A'CCwGG_T
Cuts at: 0 5351 5931
Size: 5351 580
SfcI C'TryA_G
Cuts at: 0 2497 4870 5387 5422 5853 5931
Size: 2497 2373 517 35 431 78
Fragments arranged by size:
2497 2373 517 431 78 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1301 5931
Size: 1301 4630
SfoI GGC'GCC
Cuts at: 0 1842 5931
Size: 1842 4089
SmaI CCC'GGG
Cuts at: 0 90 5931
Size: 90 5841
SmlI C'TyrA_G
Cuts at: 0 430 766 1226 2956 3110 3493 5735 5931
Size: 430 336 460 1730 154 383 2242 196
Fragments arranged by size:
2242 1730 460 430 383 336 196 154
SnaBI TAC'GTA
Cuts at: 0 396 5931
Size: 396 5535
SphI G_CATG'C
Cuts at: 0 2025 4038 5931
Size: 2025 2013 1893
Fragments arranged by size:
2025 2013 1893
StuI AGG'CCT
Cuts at: 0 131 2363 3976 5931
Size: 131 2232 1613 1955
Fragments arranged by size:
2232 1955 1613 131
StyI C'CwwG_G
Cuts at: 0 132 777 983 1025 1127 1272 1443 1826
Size: 132 645 206 42 102 145 171 383
Cuts at: 1826 1853 2306 2513 2948 3662 3837 4418 5210
Size: 27 453 207 435 714 175 581 792
Cuts at: 5210 5664 5931
Size: 454 267
Fragments arranged by size:
792 714 645 581 454 453 435 383
267 207 206 175 171 145 132 102
42 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1012 1035 1687 5164 5931
Size: 1012 23 652 3477 767
Fragments arranged by size:
3477 1012 767 652 23
TatI w'GTAC_w
Cuts at: 0 1049 1483 2907 2916 5931
Size: 1049 434 1424 9 3015
Fragments arranged by size:
3015 1424 1049 434 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2183 2215 2782 2814 5931
Size: 2183 32 567 32 3117
Fragments arranged by size:
3117 2183 567 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3211 5931
Size: 3211 2720
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 1851 2520 2628 2745 5826 5931
Size: 375 1476 669 108 117 3081 105
Fragments arranged by size:
3081 1476 669 375 117 108 105
XhoI C'TCGA_G
Cuts at: 0 766 5735 5931
Size: 766 4969 196
Fragments arranged by size:
4969 766 196
XmaI C'CCGG_G
Cuts at: 0 88 5931
Size: 88 5843
XmnI GAAnn'nnTTC
Cuts at: 0 277 3391 5931
Size: 277 3114 2540
Fragments arranged by size:
3114 2540 277
ZraI GAC'GTC
Cuts at: 0 1348 5931
Size: 1348 4583
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NdeI NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuII SacI SacII SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI SnaBI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AloI AscI AseI AsiSI BaeI
BlpI BmtI BsiEI BsiWI BssHII BstBI DraI DrdI
EagI EcoRV FseI FspAI HpaI MfeI MluI NaeI
NgoMIV NheI NotI NruI PacI PciI PmeI PsiI
PvuI RsrII SalI SgfI SgrAI SpeI SrfI SspI
SwaI XbaI