################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 37 GTAC GT^AC AluI 43 AGCT AG^CT AlwNI 340 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 8 GGTCC G^G[AT]CC BalI 201 TGGCCA TGG^CCA BbsI 216 GAAGAC GAAGAC(2/6) BbvI 292 GCTGC GCAGC(8/12) BcnI 5 CCCGG CC^[GC]GG BfaI 313 CTAG C^TAG BpuAI 216 GAAGAC GAAGAC(2/6) BsaJI 488 CCTGGG C^C[ACGT][ACGT]GG BsiYI 183 CCCAGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 183 CCCAGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 391 GAGAC GTCTC(1/5) BspHI 512 TCATGA T^CATGA BsrI 207 CCAGT ACTGG(1/-1) BstF5I 417 CATCC GGATG(2/0) BstYI 517 AGATCC [GA]^GATC[CT] Cac8I 92 GCCTGC GC[ACGT]^[ACGT]GC DraII 106 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 201 TGGCCA [CT]^GGCC[GA] EcoO109I 106 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 172 GAATTC G^AATTC Fnu4HI 292 GCTGC GC^[ACGT]GC FokI 417 CATCC GGATG(9/13) HapII 6 CCGG C^CGG HpaII 6 CCGG C^CGG ItaI 292 GCTGC GC^[ACGT]GC MaeI 313 CTAG C^TAG MflI 517 AGATCC [GA]^GATC[CT] MluNI 201 TGGCCA TGG^CCA MscI 201 TGGCCA TGG^CCA MseI 553 TTAA T^TAA MspI 6 CCGG C^CGG MwoI 59 GCTCTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 5 CCCGG CC^[GC]GG NspI 361 GCATGT [GA]CATG^[CT] RcaI 512 TCATGA T^CATGA RsaI 37 GTAC GT^AC SmlI 221 CTCAAG C^T[CT][GA]AG Tru9I 553 TTAA T^TAA TseI 292 GCTGC G^C[AT]GC TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 53 CCATCTGCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 517 AGATCC [GA]^GATC[CT] XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AcsI 172 GAATTC [GA]^AATT[CT] 319 AAATTT AlwI 249 GATCC GGATC(4/5) 518 GATCC ApoI 172 GAATTC [GA]^AATT[CT] 319 AAATTT BseRI 187 GAGGAG GAGGAG(10/8) 190 GAGGAG DdeI 40 CTCAG C^T[ACGT]AG 80 CTCAG EarI 268 CTCTTC CTCTTC(1/4) 388 GAAGAG Ksp632I 268 CTCTTC CTCTTC(1/4) 388 GAAGAG Tsp509I 173 AATT ^AATT 320 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### BssKI BstNI Cfr13I DpnII EcoRII HaeIII HinfI MboI MboII MnlI MvaI NdeII NlaIII PleI Sau3AI Sau96I ScrFI TfiI