(Circular) (Six-base) MAPSORT of: pFN21AE6075.seq Check: 9071 from: 1 to: 5674 pFN21AE6075 5674 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2526 3017 1937 Size: 589 491 4594 Fragments arranged by size: 4594 589 491 Acc65I G'GTAC_C Cuts at: 2995 2995 Size: 5674 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 3737 5121 5389 1632 Size: 629 1476 1384 268 1917 Fragments arranged by size: 1917 1476 1384 629 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 4573 5550 1203 Size: 3370 977 1327 Fragments arranged by size: 3370 1327 977 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2822 1184 Size: 1638 4036 AlwNI CAG_nnn'CTG Cuts at: 3417 4989 3417 Size: 1572 4102 ApaLI G'TGCA_C Cuts at: 2797 3731 4887 2797 Size: 934 1156 3584 Fragments arranged by size: 3584 1156 934 ApoI r'AATT_y Cuts at: 1493 2857 2983 3209 3361 5470 1493 Size: 1364 126 226 152 2109 1697 Fragments arranged by size: 2109 1697 1364 226 152 126 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2967 2999 1870 Size: 73 1024 32 4545 Fragments arranged by size: 4545 1024 73 32 BamHI G'GATC_C Cuts at: 3004 3371 3004 Size: 367 5307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2759 2995 4450 618 Size: 325 217 627 972 236 1455 1842 Fragments arranged by size: 1842 1455 972 627 325 236 217 BanII G_rGCy'C Cuts at: 1391 2055 2993 1391 Size: 664 938 4072 Fragments arranged by size: 4072 938 664 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 2650 928 Size: 971 199 552 3952 Fragments arranged by size: 3952 971 552 199 BbvCI CC'TCA_GC Cuts at: 2915 2915 Size: 5674 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2476 2510 3865 3899 1903 Size: 34 539 34 1355 34 3678 Fragments arranged by size: 3678 1355 539 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BfrBI ATG'CAT Cuts at: 2221 2221 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4285 136 Size: 107 122 71 631 605 2613 1525 Fragments arranged by size: 2613 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5669 5669 Size: 5674 BlpI GC'TnA_GC Cuts at: 2192 3091 2192 Size: 899 4775 Bme1580I G_kGCm'C Cuts at: 1790 2801 3735 4891 1790 Size: 1011 934 1156 2573 Fragments arranged by size: 2573 1156 1011 934 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2788 2929 3405 Size: 416 53 83 399 24 1371 141 476 Cuts at: 3405 4363 442 Size: 958 1753 Fragments arranged by size: 1753 1371 958 476 416 399 141 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4334 1239 Size: 3095 2579 Bpu10I CC'TnA_GC Cuts at: 2895 2915 2895 Size: 20 5654 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2066 3799 4664 4962 2066 Size: 1733 865 298 2778 Fragments arranged by size: 2778 1733 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2585 2776 4337 882 Size: 617 1086 191 1561 2219 Fragments arranged by size: 2219 1561 1086 617 191 BsaAI yAC'GTr Cuts at: 493 1204 3465 493 Size: 711 2261 2702 Fragments arranged by size: 2702 2261 711 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3481 3863 4498 275 Size: 53 83 186 936 1948 382 635 1451 Fragments arranged by size: 1948 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2877 4107 4505 4779 4926 5337 2877 Size: 1230 398 274 147 411 3214 Fragments arranged by size: 3214 1230 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5449 Fragments arranged by size: 5449 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2609 2612 2615 2932 1186 Size: 949 474 3 3 317 3928 Fragments arranged by size: 3928 949 474 317 3 3 BseYI C'CCAG_C Cuts at: 2163 2301 2548 4877 5373 2163 Size: 138 247 2329 496 2464 Fragments arranged by size: 2464 2329 496 247 138 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 3885 4913 1540 Size: 275 186 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2409 2801 3735 3820 4891 2409 Size: 392 934 85 1071 3192 Fragments arranged by size: 3192 1071 934 392 85 BsmI GAATG_Cn' Cuts at: 2613 2940 3185 3278 2613 Size: 327 245 93 5009 Fragments arranged by size: 5009 327 245 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2409 2801 2993 3735 3820 4891 Size: 399 265 354 392 192 742 85 1071 Cuts at: 4891 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 392 354 265 192 85 BspEI T'CCGG_A Cuts at: 2877 5337 2877 Size: 2460 3214 BspHI T'CATG_A Cuts at: 2409 3563 5293 2409 Size: 1154 1730 2790 Fragments arranged by size: 2790 1730 1154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3029 844 Size: 2185 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3561 1394 Size: 429 1738 3507 Fragments arranged by size: 3507 1738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4169 4343 65 Size: 1183 2921 174 1396 Fragments arranged by size: 2921 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4318 4505 1700 Size: 110 2508 187 2869 Fragments arranged by size: 2869 2508 187 110 BsrGI T'GTAC_A Cuts at: 96 2601 96 Size: 2505 3169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5674 BssSI C'ACGA_G Cuts at: 3734 4746 3734 Size: 1012 4662 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5674 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstYI r'GATC_y Cuts at: 1972 2212 3004 3371 3756 3773 5225 5311 5323 Size: 240 792 367 385 17 1452 86 12 Cuts at: 5323 5669 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 792 385 367 346 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2097 513 Size: 552 1032 4090 Fragments arranged by size: 4090 1032 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4935 Fragments arranged by size: 4935 683 56 BtsI GCAGTG_nn' Cuts at: 2390 3190 3984 4004 2390 Size: 800 794 20 4060 Fragments arranged by size: 4060 800 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DraIII CAC_nnn'GTG Cuts at: 2937 2937 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3121 4010 5639 Size: 54 1425 50 275 18 1291 889 1629 Cuts at: 5639 8 Size: 43 Fragments arranged by size: 1629 1425 1291 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2902 3604 1353 Size: 707 842 702 3423 Fragments arranged by size: 3423 842 707 702 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 3737 4334 5121 5389 1239 Size: 393 629 1476 597 787 268 1524 Fragments arranged by size: 1524 1476 787 629 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2790 2790 Size: 5674 EcoRI G'AATT_C Cuts at: 2857 2983 3361 2857 Size: 126 378 5170 Fragments arranged by size: 5170 378 126 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 4821 1678 Size: 3143 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2857 2889 2913 2945 4315 Size: 32 135 32 1285 32 24 32 1370 Cuts at: 4315 4347 4389 4421 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1370 1285 135 42 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 669 1657 1938 3018 3264 669 Size: 988 281 1080 246 3079 Fragments arranged by size: 3079 1080 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5674 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KpnI G_GTAC'C Cuts at: 2999 2999 Size: 5674 MfeI C'AATT_G Cuts at: 2954 3273 2954 Size: 319 5355 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4788 4972 1305 Size: 3483 184 2007 Fragments arranged by size: 3483 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3852 Fragments arranged by size: 3852 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2582 2681 3632 3991 4150 518 Size: 648 1416 99 951 359 159 2042 Fragments arranged by size: 2042 1416 951 648 359 159 99 MspA1I CmG'CkG Cuts at: 1606 2552 3090 3767 4915 5160 1606 Size: 946 538 677 1148 245 2120 Fragments arranged by size: 2120 1148 946 677 538 245 NcoI C'CATG_G Cuts at: 513 1065 2097 513 Size: 552 1032 4090 Fragments arranged by size: 4090 1032 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NsiI A_TGCA'T Cuts at: 2223 2223 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 2225 3032 4577 1132 Size: 97 996 807 1545 2229 Fragments arranged by size: 2229 1545 996 807 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5674 PciI A'CATG_T Cuts at: 4573 4573 Size: 5674 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5674 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2790 2790 Size: 5674 PshAI GACnn'nnGTC Cuts at: 1613 2788 1613 Size: 1175 4499 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PstI C_TGCA'G Cuts at: 830 3026 830 Size: 2196 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 PvuII CAG'CTG Cuts at: 2552 2552 Size: 5674 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 2060 Size: 5674 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 3922 5634 3922 Size: 1712 3962 SfcI C'TryA_G Cuts at: 826 1045 2833 3022 4157 4838 5029 826 Size: 219 1788 189 1135 681 191 1471 Fragments arranged by size: 1788 1471 1135 681 219 191 189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 3001 1872 Size: 1129 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 3778 4679 4941 820 Size: 197 926 102 1733 901 262 1553 Fragments arranged by size: 1733 1553 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 3598 5 Size: 47 3546 2081 Fragments arranged by size: 3546 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 2097 3113 513 Size: 552 1032 1016 3074 Fragments arranged by size: 3074 1032 1016 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2948 3701 3718 3871 4056 4501 325 Size: 1255 1368 753 17 153 185 445 1498 Fragments arranged by size: 1498 1368 1255 753 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2601 3920 5632 Size: 274 80 33 51 1443 624 1319 1712 Cuts at: 5632 96 Size: 138 Fragments arranged by size: 1712 1443 1319 624 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5674 XmaI C'CCGG_G Cuts at: 1870 2999 1870 Size: 1129 4545 XmnI GAAnn'nnTTC Cuts at: 2340 3803 2340 Size: 1463 4211 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI