################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BalI BbrPI BbsI BbvCI BcgI BfrI BglII BlnI BpuAI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV EcoT22I FseI HgaI HincII HindII HindIII HinfI HpaI KpnI KspI MamI MfeI MluI MluNI MscI MunI MvnI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 419 GTCTAC GT^[AC][GT]AC AccIII 674 TCCGGA T^CCGGA AfaI 174 GTAC GT^AC AocI 781 CCTAAGG CC^T[ACGT]AGG AspHI 287 GTGCTC G[AT]GC[AT]^C AvaII 228 GGTCC G^G[AT]CC AviII 185 TGCGCA TGC^GCA BamHI 677 GGATCC G^GATCC BanII 642 GGGCTC G[GA]GC[CT]^C BciVI 656 GTATCC GTATCC(6/5) BclI 731 TGATCA T^GATCA BcnI 411 CCGGG CC^[GC]GG BfaI 427 CTAG C^TAG BglI 623 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 402 GCTGAGC GC^T[ACGT]AGC Bpu1102I 402 GCTGAGC GC^T[ACGT]AGC BsaWI 674 TCCGGA [AT]^CCGG[AT] BseAI 674 TCCGGA T^CCGGA BsiHKAI 287 GTGCTC G[AT]GC[AT]^C BspEI 674 TCCGGA T^CCGGA BsrFI 260 GCCGGC [GA]^CCGG[CT] BstEII 837 GGTCACC G^GT[ACGT]ACC BstPI 837 GGTCACC G^GT[ACGT]ACC Bsu36I 781 CCTAAGG CC^T[ACGT]AGG CelII 402 GCTGAGC GC^T[ACGT]AGC Cfr10I 260 GCCGGC [GA]^CCGG[CT] Eco81I 781 CCTAAGG CC^T[ACGT]AGG EcoO65I 837 GGTCACC G^GT[ACGT]ACC FbaI 731 TGATCA T^GATCA FspI 185 TGCGCA TGC^GCA MaeI 427 CTAG C^TAG MaeII 217 ACGT A^CGT MroI 674 TCCGGA T^CCGGA MseI 578 TTAA T^TAA MspA1I 553 CAGCTG C[AC]G^C[GT]G NaeI 260 GCCGGC GCC^GGC NciI 411 CCGGG CC^[GC]GG NgoMIV 260 GCCGGC G^CCGGC NheI 426 GCTAGC G^CTAGC PvuII 553 CAGCTG CAG^CTG RsaI 174 GTAC GT^AC SapI 75 GCTCTTC GCTCTTC(1/4) SfaNI 151 GATGC GCATC(5/9) StuI 704 AGGCCT AGG^CCT Tru9I 578 TTAA T^TAA Tsp509I 121 AATT ^AATT ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 263 GGCGCC G[GA]^CG[CT]C 319 GGCGCC BbeI 263 GGCGCC GGCGC^C 319 GGCGCC BmyI 287 GTGCTC G[AGT]GC[ACT]^C 642 GGGCTC BsaHI 263 GGCGCC G[GA]^CG[CT]C 319 GGCGCC BsaI 141 GGTCTC GGTCTC(1/5) 738 GGTCTC BseRI 610 CTCCTC GAGGAG(10/8) 720 CTCCTC Bsp1286I 287 GTGCTC G[AGT]GC[ACT]^C 642 GGGCTC BspMI 101 ACCTGC ACCTGC(4/8) 715 ACCTGC BsrI 256 ACTGG ACTGG(1/-1) 162 CCAGT BstYI 536 AGATCC [GA]^GATC[CT] 677 GGATCC DraIII 324 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG 409 CACCGGGTG FauI 519 CCCGC CCCGC(4/6) 691 GCGGG HaeII 263 GGCGCC [GA]GCGC^[CT] 319 GGCGCC Hin1I 263 GGCGCC G[GA]^CG[CT]C 319 GGCGCC KasI 263 GGCGCC G^GCGCC 319 GGCGCC MflI 536 AGATCC [GA]^GATC[CT] 677 GGATCC MslI 12 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 129 CATTGTGGTG NarI 263 GGCGCC GG^CGCC 319 GGCGCC NcoI 17 CCATGG C^CATGG 347 CCATGG PstI 550 CTGCAG CTGCA^G 586 CTGCAG SfoI 263 GGCGCC GGC^GCC 319 GGCGCC XcmI 246 CCACCTCCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 803 CCACTGACCCTCTGG XhoII 536 AGATCC [GA]^GATC[CT] 677 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AlwI BanI BbvI BpmI BsaJI BsiYI BslI BsmAI BsmFI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfcI StyI TseI Tsp45I TspRI