################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I TseI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AcsI 37 GAATTT [GA]^AATT[CT] AfaI 423 GTAC GT^AC ApaI 412 GGGCCC GGGCC^C ApoI 37 GAATTT [GA]^AATT[CT] BamHI 573 GGATCC G^GATCC BanII 412 GGGCCC G[GA]GC[CT]^C BbvCI 498 GCTGAGG CCTCAGC(-5/-2) BmyI 412 GGGCCC G[AGT]GC[ACT]^C BsmAI 10 GTCTC GTCTC(1/5) BsmI 334 GAATGC GAATGC(1/-1) Bsp1286I 412 GGGCCC G[AGT]GC[ACT]^C BspHI 767 TCATGA T^CATGA BstAPI 784 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 63 CATCC GGATG(2/0) BstXI 659 CCAGAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 573 GGATCC [GA]^GATC[CT] DraII 411 GGGGCCC [GA]G^G[ACGT]CC[CT] EarI 853 CTCTTC CTCTTC(1/4) EcoNI 810 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 411 GGGGCCC [GA]G^G[ACGT]CC[CT] FokI 63 CATCC GGATG(9/13) Ksp632I 853 CTCTTC CTCTTC(1/4) MflI 573 GGATCC [GA]^GATC[CT] NdeI 359 CATATG CA^TATG NspI 721 ACATGC [GA]CATG^[CT] RcaI 767 TCATGA T^CATGA RsaI 423 GTAC GT^AC SfcI 740 CTGTAG C^T[GA][CT]AG XhoII 573 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 230 CCGC CCGC(-3/-1) 366 CCGC AlwI 574 GATCC GGATC(4/5) 573 GGATC BbsI 112 GTCTTC GAAGAC(2/6) 820 GTCTTC BfaI 322 CTAG C^TAG 736 CTAG BpmI 298 CTCCAG CTGGAG(16/14) 250 CTGGAG BpuAI 112 GTCTTC GAAGAC(2/6) 820 GTCTTC BseRI 726 CTCCTC GAGGAG(10/8) 808 CTCCTC BstEII 489 GGTGACC G^GT[ACGT]ACC 843 GGTCACC BstPI 489 GGTGACC G^GT[ACGT]ACC 843 GGTCACC EcoO65I 489 GGTGACC G^GT[ACGT]ACC 843 GGTCACC EcoT14I 48 CCTTGG C^C[AT][AT]GG 815 CCAAGG MaeI 322 CTAG C^TAG 736 CTAG MspA1I 329 CAGCTG C[AC]G^C[GT]G 496 CAGCTG PvuII 329 CAGCTG CAG^CTG 496 CAGCTG SfaNI 237 GATGC GCATC(5/9) 894 GCATC StyI 48 CCTTGG C^C[AT][AT]GG 815 CCAAGG XcmI 416 CCAGACGGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 659 CCAGAATTGTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AluI BsaJI BsiYI BslI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII HaeIII HinfI HphI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI Tsp45I Tsp509I TspRI