################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MaeIII MamI MfeI MflI MluI MroI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 811 AAATTT [GA]^AATT[CT] Alw44I 652 GTGCAC G^TGCAC AlwI 471 GATCC GGATC(4/5) ApaLI 652 GTGCAC G^TGCAC ApoI 811 AAATTT [GA]^AATT[CT] Asp700I 519 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 602 CCCGAG C^[CT]CG[GA]G BbvCI 672 GCTGAGG CCTCAGC(-5/-2) BclI 245 TGATCA T^GATCA BpmI 67 CTGGAG CTGGAG(16/14) BsaI 481 GGTCTC GGTCTC(1/5) BsgI 163 CTGCAC GTGCAG(16/14) BsmAI 482 GTCTC GTCTC(1/5) BsoBI 602 CCCGAG C^[CT]CG[GA]G CfoI 696 GCGC GCG^C DraII 721 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 327 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 714 GACTTGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 279 CCGTGG C^C[GA][CT]GG EarI 301 CTCTTC CTCTTC(1/4) EcoO109I 721 GGGTCCC [GA]G^G[ACGT]CC[CT] FbaI 245 TGATCA T^GATCA HhaI 696 GCGC GCG^C HinP1I 696 GCGC G^CGC HincII 684 GTCAAC GT[CT]^[GA]AC HindII 684 GTCAAC GT[CT]^[GA]AC Ksp632I 301 CTCTTC CTCTTC(1/4) MaeII 884 ACGT A^CGT PpuMI 721 GGGTCCC [GA]G^G[AT]CC[CT] PstI 626 CTGCAG CTGCA^G SapI 300 GCTCTTC GCTCTTC(1/4) ScaI 906 AGTACT AGT^ACT SfaNI 537 GATGC GCATC(5/9) SfcI 626 CTGCAG C^T[GA][CT]AG TaqI 513 TCGA T^CGA TspRI 668 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 513 TCGA T^CGA XcmI 587 CCAAGATGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 519 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 177 GTAC GT^AC 907 GTAC AluI 239 AGCT AG^CT 299 AGCT AlwNI 238 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 424 CAGACCCTG AspHI 337 GTGCTC G[AT]GC[AT]^C 652 GTGCAC BlpI 295 GCTCAGC GC^T[ACGT]AGC 755 GCTCAGC Bpu1102I 295 GCTCAGC GC^T[ACGT]AGC 755 GCTCAGC BsiHKAI 337 GTGCTC G[AT]GC[AT]^C 652 GTGCAC BsrDI 657 CATTGC GCAATG(2/0) 487 GCAATG BsrI 66 ACTGG ACTGG(1/-1) 17 CCAGT BstF5I 75 CATCC GGATG(2/0) 783 CATCC CelII 295 GCTCAGC GC^T[ACGT]AGC 755 GCTCAGC DpnII 246 GATC ^GATC 471 GATC FauI 257 GCGGG CCCGC(4/6) 451 GCGGG FokI 75 CATCC GGATG(9/13) 783 CATCC MboI 246 GATC ^GATC 471 GATC MwoI 63 GCAACTGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 619 GCTGTTCCTGC NdeII 246 GATC ^GATC 471 GATC PflMI 390 CCACTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 423 CCAGACCCTGG PleI 925 GACTC GAGTC(4/5) 605 GAGTC RsaI 177 GTAC GT^AC 907 GTAC Sau3AI 246 GATC ^GATC 471 GATC TfiI 47 GATTC G^A[AT]TC 519 GAATC Van91I 390 CCACTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 423 CCAGACCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AvaII BalI BanII BbvI BfaI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cfr13I DdeI EaeI EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeI MboII MluNI MnlI MscI MslI MvaI NlaIII NlaIV Sau96I ScrFI TseI Tsp509I