(Circular) (Six-base) MAPSORT of: pFN21AE5924.seq Check: 3700 from: 1 to: 5638 pFN21AE5924 5638 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2981 1937 Size: 1044 4594 Acc65I G'GTAC_C Cuts at: 2959 2959 Size: 5638 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2576 3701 5085 5353 1632 Size: 944 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1125 944 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 4537 5514 1203 Size: 3334 977 1327 Fragments arranged by size: 3334 1327 977 AgeI A'CCGG_T Cuts at: 4469 4469 Size: 5638 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5638 AlwNI CAG_nnn'CTG Cuts at: 2248 2434 3381 4953 2248 Size: 186 947 1572 2933 Fragments arranged by size: 2933 1572 947 186 ApaLI G'TGCA_C Cuts at: 2657 3695 4851 2657 Size: 1038 1156 3444 Fragments arranged by size: 3444 1156 1038 ApoI r'AATT_y Cuts at: 1493 2816 2947 3173 3325 5434 1493 Size: 1323 131 226 152 2109 1697 Fragments arranged by size: 2109 1697 1323 226 152 131 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2607 2963 1870 Size: 73 664 356 4545 Fragments arranged by size: 4545 664 356 73 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2959 4414 618 Size: 325 217 627 1172 1455 1842 Fragments arranged by size: 1842 1455 1172 627 325 217 BanII G_rGCy'C Cuts at: 1391 2079 2330 2902 2957 1391 Size: 688 251 572 55 4072 Fragments arranged by size: 4072 688 572 251 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4667 BbvCI CC'TCA_GC Cuts at: 2678 2678 Size: 5638 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 4740 3530 Size: 1210 4428 BclI T'GATC_A Cuts at: 1548 2250 1548 Size: 702 4936 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4249 136 Size: 107 122 71 631 605 2577 1525 Fragments arranged by size: 2577 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5633 5633 Size: 5638 BlpI GC'TnA_GC Cuts at: 2301 2761 3055 2301 Size: 460 294 4884 Fragments arranged by size: 4884 460 294 Bme1580I G_kGCm'C Cuts at: 1790 2241 2661 3699 4855 1790 Size: 451 420 1038 1156 2573 Fragments arranged by size: 2573 1156 1038 451 420 BmgBI CAC'GTC Cuts at: 1318 2890 1318 Size: 1572 4066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3369 4327 442 Size: 416 53 83 399 24 1952 958 1753 Fragments arranged by size: 1952 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 4298 1239 Size: 854 2205 2579 Fragments arranged by size: 2579 2205 854 Bpu10I CC'TnA_GC Cuts at: 2678 2678 Size: 5638 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3763 4628 4926 3763 Size: 865 298 4475 Fragments arranged by size: 4475 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2492 4301 882 Size: 617 993 1809 2219 Fragments arranged by size: 2219 1809 993 617 BsaAI yAC'GTr Cuts at: 493 1204 3429 493 Size: 711 2225 2702 Fragments arranged by size: 2702 2225 711 BsaBI GATnn'nnATC Cuts at: 5632 5632 Size: 5638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3445 3827 4462 275 Size: 53 83 186 936 1912 382 635 1451 Fragments arranged by size: 1912 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4071 4469 4743 4890 5301 4071 Size: 398 274 147 411 4408 Fragments arranged by size: 4408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2280 2310 1178 Size: 30 165 30 877 30 4506 Fragments arranged by size: 4506 877 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5638 BseYI C'CCAG_C Cuts at: 2241 2696 4841 5337 2241 Size: 455 2145 496 2542 Fragments arranged by size: 2542 2145 496 455 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 1163 Size: 573 417 4648 Fragments arranged by size: 4648 573 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3849 4877 1540 Size: 275 186 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2346 2661 3699 3784 4855 2346 Size: 315 1038 85 1071 3129 Fragments arranged by size: 3129 1071 1038 315 85 BsmI GAATG_Cn' Cuts at: 3149 3242 3149 Size: 93 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2241 2330 2346 2661 2902 2957 Size: 399 289 162 89 16 315 241 55 Cuts at: 2957 3699 3784 4855 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 315 289 241 162 89 85 55 16 BspEI T'CCGG_A Cuts at: 5301 5301 Size: 5638 BspHI T'CATG_A Cuts at: 3527 5257 3527 Size: 1730 3908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 844 Size: 2149 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3525 1394 Size: 429 1702 3507 Fragments arranged by size: 3507 1702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2499 2661 4133 4307 65 Size: 1183 1251 162 1472 174 1396 Fragments arranged by size: 1472 1396 1251 1183 174 162 BsrFI r'CCGG_y Cuts at: 1700 1810 4282 4469 1700 Size: 110 2472 187 2869 Fragments arranged by size: 2869 2472 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 3698 4710 3698 Size: 1012 4626 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633 Size: 996 367 385 17 1452 86 12 346 Cuts at: 5633 1972 Size: 1977 Fragments arranged by size: 1977 1452 996 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2284 513 Size: 552 939 280 3867 Fragments arranged by size: 3867 939 552 280 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2125 487 Size: 683 56 899 4000 Fragments arranged by size: 4000 899 683 56 BtsI GCAGTG_nn' Cuts at: 2679 3154 3948 3968 2679 Size: 475 794 20 4349 Fragments arranged by size: 4349 794 475 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DraIII CAC_nnn'GTG Cuts at: 2337 2337 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2725 4645 809 Size: 507 1409 1920 1802 Fragments arranged by size: 1920 1802 1409 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2063 2403 2496 Size: 54 1425 50 275 18 233 340 93 Cuts at: 2496 2512 3085 3974 5603 8 Size: 16 573 889 1629 43 Fragments arranged by size: 1629 1425 889 573 340 275 233 93 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 3568 1353 Size: 959 1256 3423 Fragments arranged by size: 3423 1256 959 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2576 3701 4298 5085 5353 1239 Size: 393 461 483 1125 597 787 268 1524 Fragments arranged by size: 1524 1125 787 597 483 461 393 268 EcoO109I rG'GnC_Cy Cuts at: 2727 2727 Size: 5638 EcoRI G'AATT_C Cuts at: 2947 3325 2947 Size: 378 5260 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 4785 1678 Size: 3107 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2601 2633 4279 4311 4353 Size: 32 135 32 1029 32 1646 32 42 Cuts at: 4353 4385 1373 Size: 32 2626 Fragments arranged by size: 2626 1646 1029 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2691 2982 3228 669 Size: 988 281 753 291 246 3079 Fragments arranged by size: 3079 988 753 291 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KpnI G_GTAC'C Cuts at: 2963 2963 Size: 5638 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4752 4936 1305 Size: 3447 184 2007 Fragments arranged by size: 3447 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2405 2498 2514 10 Size: 54 1425 343 573 93 16 3134 Fragments arranged by size: 3134 1425 573 343 93 54 16 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2177 2618 3596 3955 4114 518 Size: 648 879 132 441 978 359 159 2042 Fragments arranged by size: 2042 978 879 648 441 359 159 132 MspA1I CmG'CkG Cuts at: 1606 3054 3731 4879 5124 1606 Size: 1448 677 1148 245 2120 Fragments arranged by size: 2120 1448 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4147 Fragments arranged by size: 4147 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2996 4541 1132 Size: 97 1767 1545 2229 Fragments arranged by size: 2229 1767 1545 97 PasI CC'CwG_GG Cuts at: 1348 2323 1348 Size: 975 4663 PciI A'CATG_T Cuts at: 4537 4537 Size: 5638 PflMI CCAn_nnn'nTGG Cuts at: 1108 2401 2434 1108 Size: 1293 33 4312 Fragments arranged by size: 4312 1293 33 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 5638 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2727 2727 Size: 5638 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PstI C_TGCA'G Cuts at: 830 2635 2990 830 Size: 1805 355 3478 Fragments arranged by size: 3478 1805 355 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SanDI GG'GwC_CC Cuts at: 2727 2727 Size: 5638 SapI GCTCTTCn'nnn_ Cuts at: 2312 2312 Size: 5638 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 2913 3886 5598 2913 Size: 973 1712 2953 Fragments arranged by size: 2953 1712 973 SfcI C'TryA_G Cuts at: 826 1045 2631 2986 4121 4802 4993 826 Size: 219 1586 355 1135 681 191 1471 Fragments arranged by size: 1586 1471 1135 681 355 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3742 4643 4905 820 Size: 197 926 1799 901 262 1553 Fragments arranged by size: 1799 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 3562 5 Size: 47 3510 2081 Fragments arranged by size: 3510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 2004 3077 513 Size: 552 939 1073 3074 Fragments arranged by size: 3074 1073 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3665 3682 3835 4020 4465 325 Size: 1255 2085 17 153 185 445 1498 Fragments arranged by size: 2085 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2911 3884 Size: 274 80 33 51 1443 204 730 973 Cuts at: 3884 5596 96 Size: 1712 138 Fragments arranged by size: 1712 1443 973 730 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2280 2312 5598 5630 2280 Size: 32 3286 32 2288 Fragments arranged by size: 3286 2288 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5638 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2599 2599 Size: 5638 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5638 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 2528 3767 2528 Size: 1239 4399 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI